| 序号 | 专利名 | 申请号 | 申请日 | 公开（公告）号 | 公开（公告）日 | 发明人 |
|---|---|---|---|---|---|---|
| 1 | 一种对氧及甘油具有耐受性的甘油脱水酶基因及其应用 | CN201610576214.2 | 2016-07-20 | CN105950642A | 2016-09-21 | 陈振; 刘德华 |
| 本发明提供了一种对氧及甘油具有耐受性的甘油脱水酶基因及其应用，本发明的甘油脱水酶基因dhaB1B2来自百脉根根瘤菌，仅包括两个亚基，大的α亚基和小的β亚基，其核苷酸序列分别如SEQ ID NO.1‑2所示。本发明的甘油脱水酶基因dhaB1B2具有很高的催化活性，且甘油及氧对其酶活的抑制作用远远低于目前已知的其他B12‑依赖型甘油脱水酶。另外，本发明的甘油脱水酶基因可以显著提高1,3‑丙二醇和3‑羟基丙酸的产量，具有广阔的应用前景。 | ||||||
| 2 | 在蓝藻中生产1,3-丙二醇 | CN201380064756.6 | 2013-10-18 | CN104919039A | 2015-09-16 | J·W·钦; M·A·安德森; J·崔; M·施皮克 |
| 蓝藻宿主细胞被修饰以产生有用的化学品，例如1,3-丙二醇和甘油。 | ||||||
| 3 | 一种利用重组大肠杆菌合成聚3-羟基丙酸的方法 | CN201410693426.X | 2014-11-26 | CN104480055A | 2015-04-01 | 李申 |
| 一种利用重组大肠杆菌合成聚3-羟基丙酸的方法。本发明保护了一种重组大肠杆菌菌株，以及所述重组大肠杆菌菌株的构建方法，和使用该重组大肠杆菌菌株进行生物法合成聚3-羟基丙酸的方法。本发明的有益效果是：以甘油为底物生物合成P3HP的代谢途径中，NAD+作为辅酶在催化3-羟基丙醛生产3HP的同时会产生大量的NADH。NADH的升高所造成的重组菌体内氧化还原平衡失调是限制P3HP产量进一步提高，本发明所述重组大肠杆菌菌株代谢甘油生成1,3-PDO的过程会消耗NADH，因此在P3HP的合成途径中引入1,3-PDO的合成，可以平衡菌体内还原力。有效的降低质粒的丢失率，保持质粒稳定性。 | ||||||
| 4 | Recombinant microorganisms and uses therefor | US13914234 | 2013-06-10 | US09890384B2 | 2018-02-13 | Alexander Paul Mueller; Michael Koepke; Shilpa Nagaraju |
| Carboxydotrophic acetogenic microorganisms do not produce MEK and/or 2-butanol. They lack the biosynthesis pathways to make these products. In addition, they produce the intermediate (R,R)-2,3-butanediol whereas the production of MEK and 2-butanol requires production of the intermediate (R,S)-2,3-butanediol. Nonetheless, the production of MEK and/or 2-butanol can be accomplished using recombinant microorganisms adapted to express or overexpress key enzymes in the MEK and/or 2-butanol biosynthesis pathways. Such microorganisms, such as the carboxydotrophic acetogen Clostridium autoethanogenum, can ferment substrates comprising CO. The overall scheme involves the production of 2-butanol from (R,S)-2,3-butanediol and the conversion of (R)-acetoin to (S)-2,3-butanediol. These steps are involved in the production of both MEK and 2-butanol. Such fermentation methods offer a means of using carbon monoxide from industrial processes which would otherwise be released into the atmosphere and pollute the environment. | ||||||
| 5 | 3-HYDROXYPROPIONIC ACID-PRODUCING RECOMBINANT MICROORGANISM AND METHOD OF PRODUCING 3-HYDROXYPROPIONIC ACID USING THE SAME | US14411845 | 2013-07-01 | US20150240269A1 | 2015-08-27 | Chanmoo Lee; Youngsoo Kim; In Suk Choi |
| Disclosed is a method of producing 3-hydroxypropionic acid comprising the step of culturing recombinant microorganism comprising a nucleic acid sequence encoding Ilyobacter polytropus-derived glycerol dehydratase, and a nucleic acid sequence encoding dehydrogenase, which converts 3-hydroxypropionaldehyde into 3-hydroxypropionic acid, and a recombinant microorganism used therefor. According to the present invention, the production of 3-hydroxypropionic acid remarkably increases and the production amount of 1,3-propanediol (1,3-PDO), which is generated as by-products when producing 3-hydroxypropionic acid, remarkably decreases, compared to known recombinant microorganisms comprising gene expressing Klebsiella pneumonia-derived Vitamin B12-dependent glycerol dehydratase. | ||||||
| 6 | 再生可能資源から化合物を生成するための高収量経路 | JP2016515437 | 2014-09-17 | JP2016533162A | 2016-10-27 | チョカワラ、ハーシャル |
| 本開示は、1−ブタノール、酪酸、コハク酸、1,4−ブタンジオール、1−ペンタノール、ペンタン酸、グルタル酸、1,5−ペンタンジオール、1−ヘキサノール、ヘキサン酸、アジピン酸、1,6−ヘキサンジオール、6−ヒドロキシヘキサン酸、ε−カプロラクトン、6−アミノ−ヘキサン酸、ε−カプロラクタム、ヘキサメチレンジアミン、7〜25炭素長の直鎖脂肪酸および直鎖脂肪アルコール、セバシン酸またはドデカン二酸等の化合物を調製するための方法、組成物、および、天然に存在しない微生物生物であって、前記方法は、a)アルドール付加を介して、CNアルデヒドおよびピルベートをCN+3β−ヒドロキシケトン中間体に変換する工程;およびb)酵素的工程または酵素的工程と化学的工程との組み合わせを介して、該CN+3β−ヒドロキシケトン中間体を該化合物に変換する工程を含む、方法、組成物、および、天然に存在しない微生物生物に関する。【選択図】図1 | ||||||
| 7 | ３−ヒドロキシプロピオン酸の製造方法、遺伝子組換え微生物、並びに前記方法を利用したアクリル酸、吸水性樹脂、アクリル酸エステル、およびアクリル酸エステル樹脂の製造方法 | JP2014504942 | 2013-03-12 | JPWO2013137277A1 | 2015-08-03 | 洋 堀川; 正治 向山; 大祐 立岩 |
| 本発明は、効率的に3−ヒドロキシプロピオン酸(3HP)を製造する方法を提供する。本発明は、耐酸性を有する微生物に、外来のマロニルCoAレダクターゼをコードする遺伝子、もしくは外来のマロン酸セミアルデヒドデヒドロゲナーゼをコードする遺伝子および外来の3−ヒドロキシプロピオン酸デヒドロゲナーゼをコードする遺伝子を導入した第1の遺伝子組換え微生物;または耐酸性を有する微生物に、外来のグリセリンデヒドラターゼをコードする遺伝子およびグリセリンデヒドラターゼ再活性化因子をコードする遺伝子、または外来のジオールデヒドラターゼをコードする遺伝子およびジオールデヒドラターゼ再活性化因子をコードする遺伝子と、外来のアルデヒドデヒドロゲナーゼをコードする遺伝子と、を導入した第2の遺伝子組換え微生物を用いることを有する、3−ヒドロキシプロピオン酸の製造方法に関する。 | ||||||
| 8 | METHODS AND MATERIALS FOR THE BIOSYNTHESIS OF BETA HYDROXY ACIDS AND/OR DERIVATIVES THEREOF AND/OR COMPOUNDS RELATED THERETO | US16264765 | 2019-02-01 | US20190233853A1 | 2019-08-01 | Cristina Serrano Amatriain; Alexander Brett Foster; Stephen Thomas Cartman |
| Methods and materials for the production of beta hydroxy acids, such as 3-hydroxypropanoic acid (3-HP) and/or derivatives thereof and/or compounds related thereto, are provided. Also provided are products produced in accordance with these methods and materials. | ||||||
| 9 | 3-hydroxypropionaldehyde detection and extraction | US15827173 | 2017-11-30 | US10053411B2 | 2018-08-21 | Tarek Dishisha; Rajni Hatti Kaul; Roya R. R. Sardari; Bastian Grund; Oliver Thum |
| The present invention relates to a method of extracting 3-hydroxypropionaldehyde (3-HPA) and/or derivatives thereof from an aqueous solution comprising 3-HPA, the method comprising: (a) contacting the aqueous solution with chitosan and/or chitosan comprising polymers; (b) separating the 3-HPA bound chitosan and/or chitosan comprising polymers; and (c) washing the 3-HPA bound chitosan and/or chitosan comprising polymers at least once with a washing medium; wherein 3-HPA and/or derivatives thereof is in the washing medium. | ||||||
| 10 | Method for the production of 1,3-propanediol by recombinant organisms comprising genes for coenzyme B12 synthesis | US11479194 | 2006-06-29 | US20060246562A1 | 2006-11-02 | Nigel Dunn-Coleman; Anthony Gatenby; Fernando Valle |
| Recombinant organisms are provided comprising genes encoding cob(II)alamin reductase, cob(I)alamin adenosyltransferase, glycerol dehydratase and 1,3-propanediol oxidoreductase activities useful for the production of 1,3-propanediol from a variety of carbon substrates. | ||||||
| 11 | Method for the production of 1,3-propanediol by recombinant organisms comprising genes for coenzyme B12 synthesis | US09310001 | 1999-05-11 | US07074608B1 | 2006-07-11 | Nigel S. Dunn-Coleman; Anthony A. Gatenby; Fernando Valle |
| Recombinant organisms are provided comprising genes encoding aquacobalamin reductase, cob(II)alamin reductase, cob(I)alamin adenosyltransferase, glycerol dehydratase and 1,3-propanediol oxidoreductase activities useful for the production of 1,3-propanediol from a variety of carbon substrates. More specifically the following nucleotide sequences are provided: btuR, encoding the E. coli cob(I)alamin adenosyltransferase enzyme; cobA, encoding the Salmonella typhimurium cob(I)alamin adenosyltransferase enzyme; cobO, encoding the Pseudomonas denitrificans cob(I)alamin adenosyltransferase enzyme; dhaB1, encoding the α subunit of the Klebsiella glycerol dehydratase enzyme; dhaB2, encoding the β subunit of the Klebsiella glycerol dehydratase enzyme; dhaB3, encoding the γ subunit of the Klebsiella glycerol dehydratase enzyme; dhaT, encoding Klebsiella oxidoreductase enzyme; the yciK gene isolated from E. coli; the glucose isomerase promoter sequence from Streptomyces; and the N-terminal amino acid sequence for cob(II)alamin reductase from Pseudomonas denitrificans. | ||||||
| 12 | 組換え微生物およびその使用 | JP2015516270 | 2013-06-09 | JP6381525B2 | 2018-08-29 | ミューラー,アレクサンダー; コプケ,マイケル; ナガラジュ,シルパ |
| 13 | 組換え微生物およびその使用 | JP2015516270 | 2013-06-09 | JP2015518736A | 2015-07-06 | ミューラー，アレクサンダー; コプケ，マイケル; リュウ，ファンミン |
| カルボキシド栄養性(carboxydotrophic)酢酸産生性微生物は、MEKおよび/または2−ブタノールを産生しない。それらは、これらの産物を作製する生合成経路を欠く。加えて、それらは、中間体(R,R)−2,3−ブタンジオールを産生するが、MEKおよび2−ブタノールの産生には、中間体(R,S)−2,3−ブタンジオールの産生が必要とされる。それにもかかわらず、MEKおよび/または2−ブタノールの産生は、MEKおよび/または2−ブタノール生合成経路において重要な酵素を発現または過剰発現するように適合された組換え微生物を用いて達成することができる。そのような微生物、例えば、カルボキシド栄養性酢酸産生菌クロストリジウム・オートエタノゲナム(Clostridium autoethanogenum)は、COを含む基質を発酵させることができる。全体のスキームは、(R,S)−2,3−ブタンジオールからの2−ブタノールの産生、および(R)−アセトインの(S)−2,3−ブタンジオールへの変換を伴う。これらのステップは、MEKと2−ブタノールの両方の産生に関与する。そのような発酵方法は、さもなければ大気中に放出され、環境を汚染するであろう工業プロセスからの一酸化炭素を使用する手段を提供する。【選択図】図1 | ||||||
| 14 | RECOMBINANT MICROORGANISMS AND USES THEREFOR | EP13800454.4 | 2013-06-09 | EP2859089B1 | 2017-03-22 | MUELLER, Alexander; KOEPKE, Michael; NAGARAJU, Shilpa |
| Carboxydotrophic acetogenic microorganisms do not produce MEK and/or 2-butanol. They lack the biosynthesis pathways to make these products. In addition, they produce the intermediate (R,R)-2,3-butanediol whereas the production of MEK and 2-butanol requires production of the intermediate (R,S)-2,3-butanediol. Nonetheless, the production of MEK and/or 2-butanol can be accomplished using recombinant microorganisms adapted to express or overexpress key enzymes in the MEK and/or 2-butanol biosynthesis pathways. Such microorganisms, such as the carboxydotrophic acetogen Clostridium autoethanogenum, can ferment substrates comprising CO. The overall scheme involves the production of 2-butanol from (R,S)-2,3-butanediol and the conversion of (R)-acetoin to (S)-2,3-butanediol. These steps are involved in the production of both MEK and 2-butanol. Such fermentation methods offer a means of using carbon monoxide from industrial processes which would otherwise be released into the atmosphere and pollute the environment. | ||||||
| 15 | A HIGH YIELD ROUTE FOR THE PRODUCTION OF COMPOUNDS FROM RENEWABLE SOURCES | EP14846017 | 2014-09-17 | EP3047030A4 | 2017-02-22 | CHOKHAWALA HARSHAL |
| Provided herein are methods, compositions, and non-naturally occurring microbial organism for preparing compounds such as 1-butanol, butyric acid, succinic acid, 1,4-butanediol, 1-pentanol, pentanoic acid, glutaric acid, 1,5-pentanediol, 1-hexanol, hexanoic acid, adipic acid, 1,6-hexanediol, 6-hydroxy hexanoic acid, ε-Caprolactone, 6-amino-hexanoic acid, ε-Caprolactam, hexamethylenediamine, linear fatty acids and linear fatty alcohols that are between 7-25 carbons long, linear alkanes and linear α-alkenes that are between 6-24 carbons long, sebacic acid and dodecanedioic acid comprising: a) converting a CN aldehyde and pyruvate to a CN+3 β-hydroxyketone intermediate through an aldol addition; and b) converting the CN+3β-hydroxyketone intermediate to the compounds through enzymatic steps, or a combination of enzymatic and chemical steps. | ||||||
| 16 | RECOMBINANT MICROORGANISMS AND USES THEREFOR | EP13800454.4 | 2013-06-09 | EP2859089A1 | 2015-04-15 | KOEPKE, Michael; LIEW, FungMin |
| Carboxydotrophic acetogenic microorganisms do not produce MEK and/or 2-butanol. They lack the biosynthesis pathways to make these products. In addition, they produce the intermediate (R,R)-2,3-butanediol whereas the production of MEK and 2-butanol requires production of the intermediate (R,S)-2,3-butanediol. Nonetheless, the production of MEK and/or 2-butanol can be accomplished using recombinant microorganisms adapted to express or overexpress key enzymes in the MEK and/or 2-butanol biosynthesis pathways. Such microorganisms, such as the carboxydotrophic acetogen Clostridium autoethanogenum, can ferment substrates comprising CO. The overall scheme involves the production of 2-butanol from (R,S)-2,3-butanediol and the conversion of (R)-acetoin to (S)-2,3-butanediol. These steps are involved in the production of both MEK and 2-butanol. Such fermentation methods offer a means of using carbon monoxide from industrial processes which would otherwise be released into the atmosphere and pollute the environment. | ||||||
| 17 | PRODUCTION OF 3-HYDROXYPROPIONIC ACID IN RECOMBINANT ORGANISMS | EP00959652.9 | 2000-08-30 | EP1124979B1 | 2006-08-30 | SUTHERS, Patrick, F.; CAMERON, Douglas, C. |
| The production of 3-hydroxypropionic acid (3-HP) from glycerol in a bacterial host is described. 3-HP is a useful feedstock for the production of polymeric materials. The genetic engineering of a bacterial host with two enzymes is sufficient to enable production of 3-HP. One enzyme is a glycerol dehydratase and the other is an aldehyde dehydrogenase. | ||||||
| 18 | Process for preparing a meat product | EP85309255.9 | 1985-12-19 | EP0228486A1 | 1987-07-15 | Labata, Antonio Porta |
A process is disclosed for preparing a meat product involving a series of operations in which ham is deboned without tearing the tissue or aponeurosis surrounding the muscles, treated with a salting mixture incorporating enzymes, packaged in nets and placed in shaping containers and cooled for 15 to 30 days and finally subjected to a temperature of 20 to 40°C at a relative humidity of 55 to 78% for 40 to 60 days. |
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| 19 | 3-HYDROXYPROPIONALDEHYDE DETECTION AND EXTRACTION | US15827173 | 2017-11-30 | US20180170851A1 | 2018-06-21 | Tarek DISHISHA; Rajni Hatti KAUL; Roya R. R. SARDARI; Bastian GRUND; Oliver THUM |
| The present invention relates to a method of extracting 3-hydroxypropionaldehyde (3-HPA) and/or derivatives thereof from an aqueous solution comprising 3-HPA, the method comprising:(a) contacting the aqueous solution with chitosan and/or chitosan comprising polymers; (b) separating the 3-HPA bound chitosan and/or chitosan comprising polymers; and (c) washing the 3-HPA bound chitosan and/or chitosan comprising polymers at least once with a washing medium; wherein 3-HPA and/or derivatives thereof is in the washing medium. | ||||||
| 20 | 3-hydroxypropionic acid-producing recombinant microorganism and method of producing 3-hydroxypropionic acid using the same | US14411845 | 2013-07-01 | US09587255B2 | 2017-03-07 | Chanmoo Lee; Youngsoo Kim; In Suk Choi |
| Disclosed is a method of producing 3-hydroxypropionic acid comprising the step of culturing recombinant microorganism comprising a nucleic acid sequence encoding Ilyobacter polytropus-derived glycerol dehydratase, and a nucleic acid sequence encoding dehydrogenase, which converts 3-hydroxypropionaldehyde into 3-hydroxypropionic acid, and a recombinant microorganism used therefor. According to the present invention, the production of 3-hydroxypropionic acid remarkably increases and the production amount of 1,3-propanediol (1,3-PDO), which is generated as by-products when producing 3-hydroxypropionic acid, remarkably decreases, compared to known recombinant microorganisms comprising gene expressing Klebsiella pneumonia-derived Vitamin B12-dependent glycerol dehydratase. | ||||||
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