序号 专利名 申请号 申请日 公开(公告)号 公开(公告)日 发明人
1 一种获得蛋白质解物的方法 CN97194838.0 1997-05-20 CN1099839C 2003-01-29 P·M·尼尔森
发明涉及一种获得用作调味剂蛋白质解物的方法。更特别的,本发明提供了从蛋白质底物获得富含游离谷酸和/或肽结合的谷氨酸残基的水解物的一种方法,该方法包括将该底物进行脱酰胺处理且将该底物经过一种特异性作用蛋白水解酶的作用的步骤。
2 一种获得蛋白质解物的方法 CN01119457.X 1997-05-20 CN1326687A 2001-12-19 P·M·尼尔森
发明涉及一种获得用作调味剂蛋白质解物的方法。更特别的,本发明提供了从蛋白质底物获得富含游离谷酸和/或肽结合的谷氨酸残基的水解物的一种方法,该方法包括将该底物进行脱酰胺处理且将该底物经过一种特异性作用蛋白水解酶的作用的步骤。
3 一种获得蛋白质解物的方法 CN97194838.0 1997-05-20 CN1219106A 1999-06-09 P·M·尼尔森
发明涉及一种获得用作调味剂蛋白质解物的方法。更特别的,本发明提供了从蛋白质底物获得富含游离谷酸和/或肽结合的谷氨酸残基的水解物的一种方法,该方法包括将该底物进行脱酰胺处理且将该底物经过一种特异性作用蛋白水解酶的作用的步骤。
4 Carboxypeptidases and nucleic acids encoding same US11073247 2005-03-04 US20050175736A1 2005-08-11 Alexander Blinkovsky; Randy Berka; Michael Rey; Elizabeth Golightly; Alan Klotz; Thomas Mathisen; Claus Dambmann
The present invention relates to polypeptides having carboxypeptidase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing the polypeptides. The present invention further relates to methods of obtaining protein hydrolysates useful as flavor improving agents.
5 Method of obtaining protein hydrolysates US188985 1998-11-09 US6036983A 2000-03-14 Per Munk Nielsen
The present invention relates to a method of obtaining protein hydrolysates useful as flavoring agents. More specifically, the invention provides a method of obtaining from a proteinaceous sustrate a hydrolysate enriched in free glutamic acid and/or peptide bound glutamic acid residues, which method comprises the steps of subjecting the substrate to a deamidation process, and subjecting the substrate to the action of a specific acting proteolytic enzyme.
6 Carboxypeptidases and nucleic acids encoding same US11073247 2005-03-04 US07091002B2 2006-08-15 Alexander Blinkovsky; Randy Berka; Michael Rey; Elizabeth Golightly; Alan Klotz; Thomas Erik Mathisen; Claus Dambmann
The present invention relates to polypeptides having carboxypeptidase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing the polypeptides. The present invention further relates to methods of obtaining protein hydrolysates useful as flavor improving agents.
7 Carboxypeptidases and nucleic acids encoding the same US08943714 1997-10-03 US06187578B1 2001-02-13 Alexander Blinkovsky; Randy Berka; Michael Rey; Elizabeth Golightly; Alan Klotz; Thomas Erik Mathisen; Claus Dambmann; Kimberly M. Brown
The present invention relates to polypeptides having carboxypeptidase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing the polypeptides. The present invention further relates to methods of obtaining protein hydrolysates useful as flavor improving agents.
8 How to obtain a protein hydrolyzate JP54141497 1997-05-20 JP2000515003A 2000-11-14 ムンク ニエルセン,ペール
(57)【要約】 本発明はフレーバリング剤として有用なタンパク質加分解物を得る方法に関する。 より詳細には、本発明はタンパク性基質から遊離グルタミン酸及び/またはグルタミン酸残基含有ペプチドに富んだ加水分解物を得る方法を提供し、その方法は前記基質を脱アミド化過程にかける工程及び前記基質を特異的に作用するタンパク質分解性酵素の作用にかける工程を含むものである。
9 Leader arrangement for promotng secretion of gene product JP5928784 1984-03-27 JPS6054686A 1985-03-29 INSONII ATOKINSON; NAIJIERU PIITAA MINTON; ROJIYAA FURANKURIN SHIEAUTSUDO
A recombinant DNA transfer vector contains a leader sequence polynucleotide which codes for a signal polypeptide of formula I, Preferably the transfer vector is a plasmid. In one preferred embodiment the leader sequence polynucleotide is downstream of and in reading phase with a bacterial or yeast promotor and a ribosome binding site, and upstream of and in reading phase with a structural gene. The structural gene may be, for example, the carboxypeptidase G2(CPG2) gene from the chromosomal DNA of Pseudomonas species strain RS - 16. Examples of plasmids containing the leader sequence polynucleotide and the CPG2 gene are pNM1, pNM111, pNM14, pNM21, pNM22, pNM31, pNM32 and pLEC3.
10 A METHOD OF OBTAINING PROTEIN HYDROLYSATES EP97921649.6 1997-05-20 EP0969736A1 2000-01-12 NIELSEN, Per, Munk
The present invention relates to a method of obtaining protein hydrolysates useful as flavoring agents. More specifically, the invention provides a method of obtaining from a proteinaceous substrate a hydrolysate enriched in free glutamic acid and/or peptide bound glutamic acid residues, which method comprises the steps of subjecting the substrate to a deamidation process, and subjecting the substrate to the action of a specific acting proteolytic enzyme.
11 A leader sequence to promote the secretion of gene products EP84301468.9 1984-03-06 EP0121352B1 1988-01-07 Atkinson, Anthony; Minton, Nigel Peter; Sherwood, Roger Franklin
12 A METHOD OF OBTAINING PROTEIN HYDROLYSATES EP97921649.6 1997-05-20 EP0969736B1 2006-06-28 NIELSEN, Per, Munk
The present invention relates to a method of obtaining protein hydrolysates useful as flavoring agents. More specifically, the invention provides a method of obtaining from a proteinaceous substrate a hydrolysate enriched in free glutamic acid and/or peptide bound glutamic acid residues, which method comprises the steps of subjecting the substrate to a deamidation process, and subjecting the substrate to the action of a specific acting proteolytic enzyme.
13 A leader sequence to promote the secretion of gene products EP84301468.9 1984-03-06 EP0121352A1 1984-10-10 Atkinson, Anthony; Minton, Nigel Peter; Sherwood, Roger Franklin

A recombinant DNA transfer vector contains a leader sequence polynucleotide which codes for a signal polypeptide of formula 1,

Preferably the transfer vector is a plasmid. In one preferred embodiment the leader sequence polynucleotide is downstream of and in reading phase with a bacterial or yeast promotorand a ribosome binding site, and upstream of and in reading phase with a structural gene. The structural gene may be, for example, the carboxypeptidase G2(CPG2) gene from the chromosomal DNA of Pseudomonas species strain RS - 16. Examples of plasmids containing the leader sequence polynucleotide and the CPG2 gene are pNM1, pNM111, pNM14, pNM21, pNM22, pNM31, pNM32 and pLEC3.

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