子分类：
| 序号 | 专利名 | 申请号 | 申请日 | 公开（公告）号 | 公开（公告）日 | 发明人 |
|---|---|---|---|---|---|---|
| 1 | 一种用于体外同源重组的蛋白酶组合物、试剂盒及方法 | CN201610519079.8 | 2016-07-04 | CN106119222A | 2016-11-16 | 张瑞 |
| 本发明公开了一种可用于体外同源重组的混合蛋白酶体系，包括基于该体系的体外同源重组无缝克隆试剂盒及其使用方法。本发明中混合蛋白酶体系可在37‑55℃下对目标片段(单片段及多片段)进行重组反应，与其它相同功能的酶或酶体系相比，本发明的混合蛋白酶体系在37‑55℃下具有较高的活性，从而提高了重组反应的特异性，可有效地避免突变的产生，且反应完成后无需进行冰浴即可直接进行转化实验，极大地提高了反应的特异性、准确性并且简化了实验操作。 | ||||||
| 2 | REF nuclease for site-specific REF-mediated DNA cleavage | US14336828 | 2014-07-21 | US09611470B2 | 2017-04-04 | Michael Matthew Cox; Angela Jo Gruber; Tayla Maria Olsen |
| Purified Ref polypeptides with increased nuclease site-specific targeting activity, recombinant nucleic acids and cells for expression of such Ref polypeptides, and methods for using the Ref polypeptides in combination with RecA protein and variants thereof to effect targeted nuclease cleavage of a DNA duplex are disclosed. | ||||||
| 3 | エラーを最少限に抑える核酸分子の合成のための材料及び方法 | JP2017245919 | 2017-12-22 | JP2018068311A | 2018-05-10 | ギブソン ダニエル; カイアッツァ ニッキー; リチャードソン トビー |
| 【課題】核酸分子のエラー訂正に有用な材料及び方法の提供。 【解決手段】(a)所望の配列の核酸分子と所望の配列に対して1つ以上のヌクレオチドミスマッチを有する核酸分子と含む第1の複数の二本鎖核酸分子を得る工程と、(b)一方向性のミスマッチエンドヌクレアーゼ活性を有し、特定の及び核酸配列又は特定のアミノ酸配列に対して夫々90%以上の同一性を有する1つ以上の分子と前記核酸分子を反応させて、第1の複数の二本鎖核酸分子を断片化する工程と、(c)(b)の断片化二本鎖核酸分子と、(b)の一方向性ミスマッチエンドヌクレアーゼ活性と同じ方向性のエキソヌクレアーゼ活性を有する1つ以上の分子とを反応させて、ヌクレオチドミスマッチを取り除いて、断片化したエラーがない二本鎖核酸分子を提供する工程と、工程(a)の前に所望の配列の二本鎖核酸分子を増幅する工程を更に、を含む、核酸分子のエラー訂正の方法。 【選択図】なし |
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| 4 | エラーを最少限に抑える核酸分子の合成のための材料及び方法 | JP2014555797 | 2013-02-01 | JP2015509005A | 2015-03-26 | ダニエル ギブソン; ニッキー カイアッツァ; トビー リチャードソン |
| 核酸分子のエラー訂正に有用な材料及び方法を提供する。ヌクレオチドミスマッチを有する第1の複数の二本鎖核酸分子を、分子の末端又は末端の近傍にてミスマッチを有する二本鎖核酸分子を残す一方向性のミスマッチエンドヌクレアーゼ活性を有する分子への暴露によって断片化する。次いでその核酸分子を一方向性のエキソヌクレアーゼ活性を有する分子に暴露してミスマッチであるヌクレオチドを取り除く。次いで欠けているヌクレオチドを、たとえば、DNAポリメラーゼ活性を有する分子の作用によって埋めることができる。結果はヌクレオチドミスマッチの頻度が低い二本鎖核酸分子である。提供されるのはまた、ミスマッチエンドヌクレアーゼをコードする新規の核酸配列、それによってコードされるポリペプチド、同様に核酸構築物、遺伝子導入細胞、及びそれらの種々の組成物である。 | ||||||
| 5 | MATERIALS AND METHODS FOR THE SYNTHESIS OF ERROR-MINIMIZED NUCLEIC ACID MOLECULES | US15667515 | 2017-08-02 | US20180051280A1 | 2018-02-22 | Daniel G. Gibson; Nicky Caiazza; Toby H. Richardson |
| The present invention provides materials and methods useful for error correction of nucleic acid molecules. In one embodiment of the invention, a first plurality of double-stranded nucleic acid molecules having a nucleotide mismatch are fragmented by exposure to a molecule having unidirectional mismatch endonuclease activity. The nucleic acid molecules are cut at the mismatch site or near the mismatch site, leaving a double-stranded nucleic acid molecule having a mismatch at the end or near end of the molecule. The nucleic acid molecule is then exposed to a molecule having unidirectional exonuclease activity to remove the mismatched nucleotide. The missing nucleotides can then be filled in by the action of, e.g., a molecule having DNA polymerase activity. The result is double-stranded nucleic acid molecules with a decreased frequency of nucleotide mismatches. Also provided are novel nucleic acid sequences encoding mismatch endonucleases, polypeptides encoded thereby, as well as nucleic acid constructs, transgenic cells, and various compositions thereof. | ||||||
| 6 | Ref nuclease for site specific Ref-mediated DNA cleavage | US15455255 | 2017-03-10 | US09752133B2 | 2017-09-05 | Michael M. Cox; Angela J. Gruber; Tayla M. Olsen |
| Purified Ref polypeptides with increased nuclease site-specific targeting activity, recombinant nucleic acids and cells for expression of such Ref polypeptides, and methods for using the Ref polypeptides in combination with RecA protein and variants thereof to effect targeted nuclease cleavage of a DNA duplex are disclosed. | ||||||
| 7 | REF NUCLEASE FOR SITE SPECIFIC REF-MEDIATED DNA CLEAVAGE | US15455255 | 2017-03-10 | US20170183640A1 | 2017-06-29 | Michael M. Cox; Angela J. Gruber; Tayla M. Olsen |
| Purified Ref polypeptides with increased nuclease site-specific targeting activity, recombinant nucleic acids and cells for expression of such Ref polypeptides, and methods for using the Ref polypeptides in combination with RecA protein and variants thereof to effect targeted nuclease cleavage of a DNA duplex are disclosed. | ||||||
| 8 | REF Nuclease for Site-Specific REF-Mediated DNA Cleavage | US14336828 | 2014-07-21 | US20150023943A1 | 2015-01-22 | Michael Matthew Cox; Angela Jo Gruber; Tayla Maria Olsen |
| Purified Ref polypeptides with increased nuclease site-specific targeting activity, recombinant nucleic acids and cells for expression of such Ref polypeptides, and methods for using the Ref polypeptides in combination with RecA protein and variants thereof to effect targeted nuclease cleavage of a DNA duplex are disclosed. | ||||||
| 9 | MATERIALS AND METHODS FOR THE SYNTHESIS OF ERROR-MINIMIZED NUCLEIC ACID MOLECULES | EP13743710 | 2013-02-01 | EP2809795A4 | 2016-03-16 | GIBSON DANIEL; CAIAZZA NICKY; RICHARDSON TOBY |
| The present invention provides materials and methods useful for error correction of nucleic acid molecules. In one embodiment of the invention, a first plurality of double-stranded nucleic acid molecules having a nucleotide mismatch are fragmented by exposure to a molecule having unidirectional mismatch endonuclease activity. The nucleic acid molecules are cut at the mismatch site or near the mismatch site, leaving a double-stranded nucleic acid molecule having a mismatch at the end or near end of the molecule. The nucleic acid molecule is then exposed to a molecule having unidirectional exonuclease activity to remove the mismatched nucleotide. The missing nucleotides can then be filled in by the action of, e.g., a molecule having DNA polymerase activity. The result is double-stranded nucleic acid molecules with a decreased frequency of nucleotide mismatches. Also provided are novel nucleic acid sequences encoding mismatch endonucleases, polypeptides encoded thereby, as well as nucleic acid constructs, transgenic cells, and various compositions thereof. | ||||||
| 10 | MATERIALS AND METHODS FOR THE SYNTHESIS OF ERROR-MINIMIZED NUCLEIC ACID MOLECULES | EP13743710.9 | 2013-02-01 | EP2809795A1 | 2014-12-10 | GIBSON, Daniel; CAIAZZA, Nicky; RICHARDSON, Toby |
| The present invention provides materials and methods useful for error correction of nucleic acid molecules. In one embodiment of the invention, a first plurality of double-stranded nucleic acid molecules having a nucleotide mismatch are fragmented by exposure to a molecule having unidirectional mismatch endonuclease activity. The nucleic acid molecules are cut at the mismatch site or near the mismatch site, leaving a double-stranded nucleic acid molecule having a mismatch at the end or near end of the molecule. The nucleic acid molecule is then exposed to a molecule having unidirectional exonuclease activity to remove the mismatched nucleotide. The missing nucleotides can then be filled in by the action of, e.g., a molecule having DNA polymerase activity. The result is double-stranded nucleic acid molecules with a decreased frequency of nucleotide mismatches. Also provided are novel nucleic acid sequences encoding mismatch endonucleases, polypeptides encoded thereby, as well as nucleic acid constructs, transgenic cells, and various compositions thereof. | ||||||
| 11 | 뉴클레아제가 처리된 세균 유래 세포밖 소포체를 이용한 세균 동정 방법 | KR1020150030615 | 2015-03-04 | KR1020160107632A | 2016-09-19 | 고용송; 윤예진 |
| 본발명은뉴클레아제를처리에의해외부의핵산이제거된세포밖소포체의핵산을이용하여세균을동정하는방법에관한것이다. 본발명의방법에의하면, 세균유래세포밖소포체에뉴클레아제를처리하여세포밖소포체외부의핵산을제거함으로써세포밖소포체를이용한세균의동정방법의사용가능성을높일수 있으며, 상기세균동정방법의정확성을향상시킬수 있다. | ||||||
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