| 序号 | 专利名 | 申请号 | 申请日 | 公开（公告）号 | 公开（公告）日 | 发明人 |
|---|---|---|---|---|---|---|
| 1 | Surface expression vectors having pgsbca the gene coding poly-gamma-glutamate synthetase, and a method for expression of target protein at the surface of microorganism using the vector | US10484605 | 2004-02-12 | US20040253704A1 | 2004-12-16 | Moon-Hee Sung; Seung-Pyo Hong; Jong-Su Lee; Chang-Min Jung; Chul-Joong Kim; Kenji Soda; Makoto Ashiuchi |
| The present invention relates to a surface expression vector having pgsBCA, a gene coding poly-gamma-glutamate synthetase and a method for expression of target protein at the surface of microorganism using the vector. The vector, in which foreign genes are inserted, transforms microorganisms and makes foreign proteins expressed stably on the surface of microorganisms. | ||||||
| 2 | PgsA | US09026017 | 1998-02-20 | US06448037B1 | 2002-09-10 | David John Holmes; Chantal Myriam Petit; Christopher Michael Traini; Richard Lloyd Warren |
| The invention provides pgsA polypeptides and polynucleotides encoding pgsA polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing pgsA polypeptides to screen for antibacterial compounds. | ||||||
| 3 | NUCLEOTIDE SEQUENCES WHICH CODE FOR THE PGSA2 GENE | EP01940367.4 | 2001-04-26 | EP1278865A1 | 2003-01-29 | Nampoothiri, Madhavan; Möckel, Bettina; Pfefferle, Walter; Eggeling, Lothar; Sahm, Hermann |
| The invention relates to a genetically modified coryneform bacterium, the pgsA2 gene of which is enhanced, and an isolated polynucleotide which codes for CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase from coryneform bacteria, and also a process for the fermentative preparation of L-amino acids with enhancement of the pgsA2 gene in the bacteria and the use of the polynucleotide as a primer or hydridization probe. | ||||||
| 4 | SURFACE EXPRESSION VECTORS HAVING pgsBCA, THE GENE CODING POLY-GAMMA-GLUTAMATE SYNTHETASE, AND A METHOD FOR EXPRESSION OF TARGET PROTEIN AT THE SURFACE OF MICROORGANISM USING THE VECTOR | EP02755966 | 2002-08-09 | EP1421195A4 | 2005-04-27 | SUNG MOON-HEE; HONG SEUNG-PYO; LEE JONG-SU; JUNG CHANG-MIN; KIM CHUL-JOONG; SODA KENJI; ASHIUCHI MAKOTO |
| 5 | NOVEL 27411, 23413, 22438, 23553, 25278, 26212, NARC SC1, NARC 10A, NARC 1, NARC 12, NARC 13, NARC17, NARC 25, NARC 3, NARC 4, NARC 7, NARC 8, NARC 11, NARC 14A, NARC 15, NARC 16, NARC 19, NARC 20, NARC 26, NARC 27, NARC 28, NARC 30, NARC 5, NARC 6, NARC 9, NARC 10C, NARC 8B, NARC 9, NARC2A, NARC 16B, NARC 1C, NARC 1A, NARC 25, 86604 AND 32222 MOLECULES AND USES THEREFOR | US12817236 | 2010-06-17 | US20100291099A1 | 2010-11-18 | Maria A. Glucksmann; Mark J. Williamson; Fong-Ying Tsai; Laura A. Rudolph-Owen; Rosanna Kapeller-Libermann; Rachel E. Meyers; Lillian Wei-Ming Chiang; John Joseph Hunter |
| The invention provides isolated nucleic acids molecules and proteins, designated 27411, 23413, 22438, 23553, 25278, 26212, NARC SC1, NARC 20A, NARC 1, NARC 12, NARC 13, NARC17, NARC 25, NARC 3, NARC 4, NARC 7, NARC 8, NARC 11, NARC 14A, NARC 15, NARC 16, NARC 19, NARC 20, NARC 26, NARC 27, NARC 28, NARC 30, NARC 5, NARC 6, NARC 9, NARC 10C, NARC 8B, NARC 9, NARC2A, NARC 16B, NARC 1C, NARC 1A, NARC 25, 86604 and 32222 nucleic acid molecules and proteins. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing said nucleic acid molecules, host cells into which the expression vectors have been introduced, nonhuman transgenic animals in which a said genes have been introduced or disrupted, fusion proteins, antigenic peptides and antibodies to said proteins. Diagnostic and therapeutic methods utilizing compositions of the invention are also provided. | ||||||
| 6 | Surface expression vectors having pgsBCA the gene coding poly-gamma-glutamate synthetase, and a method for expression of target protein at the surface of microorganism using the vector | US10484605 | 2002-08-09 | US07553636B2 | 2009-06-30 | Moon-Hee Sung; Seung-Pyo Hong; Jong-Su Lee; Chang-Min Jung; Chul-Joong Kim; Kenji Soda; Makoto Ashiuchi |
| The present invention relates to a surface expression vector having pgsBCA, a gene coding poly-gamma-glutamate synthetase and a method for expression of target protein at the surface of microorganism using the vector. The vector, in which foreign genes are inserted, transforms microorganisms and makes foreign proteins expressed stably on the surface of microorganisms. | ||||||
| 7 | Nucleotide sequences which code for the pgsA2 gene | US09855835 | 2001-05-16 | US20020155555A1 | 2002-10-24 | Madhavan Nampoothiri K.; Bettina Mockel; Walter Pfefferle; Lothar Eggeling; Hermann Sahm |
| The invention relates to a genetically modified coryneform bacterium, the pgsA2 gene of which is amplified, and an isolated polynucleotide which codes for CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase from coryneform bacteria, and also a method for the fermentative preparation of L-amino acids with amplification of the pgsA2 gene in the bacteria and the use of the polynucleotide as a primer or hydridization probe. | ||||||
| 8 | Phosphatidylglycerophosphate synthase from Staphylococcus aureus | US09631547 | 2000-08-03 | US06458572B1 | 2002-10-01 | David John Holmes; Chanal Myriam Petit; Christopher Michael Traini; Richard Lloyd Warren |
| The invention provides Phosphatidylglycerophosphate Synthase polypeptides from Staphylococcus aureus (“pgsA polypeptides”). Also provided are methods for utilizing pgsA polypeptides to screen for antibacterial compounds. | ||||||
| 9 | pgsBCA, vector for surface expression with a gene encoding a polypeptide -γ- glutamic acid synthetase, and microbial surface expression method of a target protein using the same | JP2003519489 | 2002-08-09 | JP4458844B2 | 2010-04-28 | キム・チュルジョン; ジュン・チャンミン; スン・モンヘ; ポ・ハリョン; ホン・ソンピョ; リー・ジョンス; 健次 左右田; 誠 芦内 |
| 10 | pgsBCA, vector for surface expression with a gene encoding a polypeptide -γ- glutamic acid synthetase, and microbial surface expression method of a target protein using the same | JP2003519489 | 2002-08-09 | JP2005500054A | 2005-01-06 | キム・チュルジョン; ジュン・チャンミン; スン・モンヘ; ホン・ソンピョ; リー・ジョンス; 健次 左右田; 誠 芦内 |
| 本発明は、pgsBCA、ポリ-γ-グルタミン酸シンテターゼをコードする遺伝子を有する表面発現ベクターおよび当該ベクターを用い微生物の表面に標的タンパク質を発現させる方法に関する。 外来遺伝子が挿入されているベクターにより、微生物は形質転換し、外来タンパク質は微生物の表面で安定して発現する。 | ||||||
| 11 | New pgsA | JP2000532503 | 1999-02-12 | JP2002504321A | 2002-02-12 | クリストファー・エム・トレイニ; シャンタル・エム・プティ; デイビッド・ジェイ・ホームズ; リチャード・エル・ウォーレン |
| (57)【要約】 本発明はｐｇｓＡポリペプチドおよびｐｇｓＡポリペプチドをコードするポリヌクレオチドならびに組換え技法によるかかるポリペプチドの製法を提供する。 さらに、ｐｇｓＡポリペプチドを用いて抗菌化合物をスクリーニングする方法を提供する。 | ||||||
| 12 | SURFACE EXPRESSION VECTORS HAVING pgsBCA, THE GENE CODING POLY-GAMMA-GLUTAMATE SYNTHETASE, AND A METHOD FOR EXPRESSION OF TARGET PROTEIN AT THE SURFACE OF MICROORGANISM USING THE VECTOR | EP02755966.5 | 2002-08-09 | EP1421195B1 | 2006-10-18 | SUNG, Moon-Hee; HONG, Seung-Pyo; LEE, Jong-Su; JUNG, Chang-Min; KIM, Chul-Joong; SODA, Kenji; ASHIUCHI, Makoto |
| The present invention relates to a surface expression vector having pgsBCA, a gene coding poly-gamma-glutamate synthetase and a method for expression of target protein at the surface of microorganism using the vector. The vector, in which foreign genes are inserted, transforms microorganisms and makes foreign proteins expressed stably on the surface of microorganisms. | ||||||
| 13 | SURFACE EXPRESSION VECTORS HAVING pgsBCA, THE GENE CODING POLY-GAMMA-GLUTAMATE SYNTHETASE, AND A METHOD FOR EXPRESSION OF TARGET PROTEIN AT THE SURFACE OF MICROORGANISM USING THE VECTOR | EP02755966.5 | 2002-08-09 | EP1421195A1 | 2004-05-26 | SUNG, Moon-Hee; HONG, Seung-Pyo; LEE, Jong-Su; JUNG, Chang-Min; KIM, Chul-Joong; SODA, Kenji; ASHIUCHI, Makoto |
| The present invention relates to a surface expression vector having pgsBCA, a gene coding poly-gamma-glutamate synthetase and a method for expression of target protein at the surface of microorganism using the vector. The vector, in which foreign genes are inserted, transforms microorganisms and makes foreign proteins expressed stably on the surface of microorganisms. | ||||||
| 14 | NOVEL pgsA | EP99906980.0 | 1999-02-12 | EP1054953A1 | 2000-11-29 | PETIT, Chantal, M.; WARREN, Richard, L.; TRAINI, Christopher, M.; HOLMES, David, J. |
| The invention provides pgsA polypeptides and polynucleotides encoding pgsA polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing pgsA polypeptides to screen for antibacterial compounds. | ||||||
| 15 | 바실러스 속 균주 유래의 폴리감마글루탐산 합성유전자를이용한 표면 발현용 벡터 및 이를 이용한 단백질의 미생물표면 발현 방법 | KR1020037016665 | 2002-08-09 | KR100469800B1 | 2005-02-02 | 성문희; 홍승표; 이종수; 정창민; 김철중; 부하령; 소다겐지; 아시우치마코토 |
| The present invention relates to a surface expression vector having pgsBCA, a gene coding poly-gamma-glutamate synthetase and a method for expression of target protein at the surface of microorganism using the vector. The vector, in which foreign genes are inserted, transforms microorganisms and makes foreign proteins expressed stably on the surface of microorganisms. | ||||||
| 16 | 바실러스 속 균주 유래의 폴리감마글루탐산 합성유전자를이용한 표면 발현용 벡터 및 이를 이용한 단백질의 미생물표면 발현 방법 | KR1020037016665 | 2002-08-09 | KR1020040032824A | 2004-04-17 | 성문희; 홍승표; 이종수; 정창민; 김철중; 부하령; 소다겐지; 아시우치마코토 |
| PURPOSE: A surface expression vector including pgsBCA, a gene encoding poly-gamma-glutamate synthetase, and a method for expressing a target protein on the surface of a microorganism using the same are provided. The surface expression vector can be effectively used for the stable and easily detachable surface expression of various exogenous proteins onto the cell surface regardless of the cell cycle. CONSTITUTION: Surface expression vectors for producing a protein on a microbial surface contains one or more than two genes selected among pgsB, pgsC and pgsA, encoding a poly-γ-glutamate synthetase complex, wherein the gene is derived from a Bacillus sp. strain producing poly-γ-glutamate synthetase; and the nucleotide sequences of pgsB, pgsC and pgsA genes are homologous to those of SEQ ID NO: 1, SEQ ID NO: 2, and SEQ ID NO: 3, respectively, with an 80% homology. A method for expressing a target protein on the surface of a microorganism comprises the steps of: (a) constructing a recombinant expression vector by inserting a gene encoding the target protein into the surface expression vector; (b) transforming a Gram-negative host cell with the recombinant vector; and cultivating the transformed host cell and expressing the target protein on the surface of the host cell. | ||||||
QQ群二维码
意见反馈
意见反馈