| 序号 | 专利名 | 申请号 | 申请日 | 公开（公告）号 | 公开（公告）日 | 发明人 |
|---|---|---|---|---|---|---|
| 1 | 类固醇生物化学氧化的方法和用于此方法中的遗传工程方法产生的细胞 | CN89104208.3 | 1989-05-06 | CN1038667A | 1990-01-10 | 荷曼·斯利杰克惠斯; 格拉杜斯·考尼利斯·玛利亚·塞尔坦; 埃利克·巴斯提安·思马尔 |
| 本发明提供了含有新的表达基因盒的遗传工程方法得到的宿主细胞，它能完成类固醇的生物化学氧化作用，尤其是用导入了编码了与胆甾醇转化为氢化可的松的生物途径有关的蛋白质的DNA的细胞来完成氧化作用。合适的宿主细胞有杆菌属(Bacillus)、酵母菌属(Saccharomgces)或Kluyveromyces的菌种。这些新的宿主细胞适合于胆甾醇，导甾烯醇酮、孕酮、17α-孕酮和11-脱氧皮质醇的微生物氧化作用，它们是所述生物途径的中间产物。这些新的表达基因盒在最终产生一个一步完成胆甾醇向氢化可的松转化的多基因系统中也是有用的。 | ||||||
| 2 | 类固醇多步氧化方法和所用的遗传工程细胞 | CN89108378.2 | 1989-09-23 | CN1042567A | 1990-05-30 | 哈门·斯利克会斯; 格拉杜斯·考那利斯·玛丽·塞尔顿; 埃里克·巴斯廷·斯迈尔 |
| 本发明提供了一种基团工程构建的宿主细胞，该细胞能够同时氧化类固醇，优选同时导入17α-羟基和C21-羟基基团。特别是氧化是用细胞进行的，在该细胞中插入有编码至少两种涉及胆固醇向氢化可的松生化转化的蛋白质。合适的宿主细胞包括杆菌属、酵母属或Kluyreromyces菌珠。该新的宿主细胞适于胆固醇、孕甾烯醇酮、孕甾酮和17α-羟基-孕甾酮的生化氧化，它们都是所说的生化转化的中间产物。该宿主细胞还可用于最终制备多基因系统，该系统能一步将胆固醇转化为氢化可的松。 | ||||||
| 3 | Process for oxidation of steroids and genetically engineered cells used therein | US09098877 | 1998-06-17 | US06632633B1 | 2003-10-14 | Herman Slijkhuis; Eric Bastiaan Smaal; Gerardus Cornelis Maria Selten |
| An expression cassette, operable in a recombinant host, comprising a heterologous DNA coding sequence encoding a protein, which is functional, alone or in cooperation with one or more additional proteins of catalyzing an oxidation step in the biological pathway, for conversion of cholesterol into hydrocortisone, which step is selected from the group consisting of: the conversion of cholesterol to pregnenolone; the conversion of pregnenolone to progesterone; the conversion of progesterone to 17&agr;-hydroxyprogesterone; the conversion of 17&agr;-hydroxyprogesterone to cortexolone; the conversion of cortexolone to hydrocortisone, and the corresponding control sequences effective in that host. | ||||||
| 4 | Expression cassette operable in a recombinant host | US418085 | 1995-04-06 | US5869283A | 1999-02-09 | Herman Slijkhuis; Eric Bastiaan Smaal; Gerardus Cornelis Maria Selten |
| An expression cassette, operable in a recombinant host, comprising a heterologous DNA coding sequence encoding a protein, which is functional, alone or in cooperation with one or more additional proteins, of catalyzing an oxidation step in the biological pathway for conversion of cholesterol into hydrocortisone, which step is selected from the group consisting of:the conversion of cholesterol to pregnenolone;the conversion of pregnenolone to progesterone;the conversion of progesterone to 17.alpha.-hydroxy-progesterone;the conversion of 17.alpha.-hydroxyprogesterone to cortexolone;the conversion of cortexolone to hydrocortisone, and thecorresponding control sequences effective in said host. | ||||||
| 5 | METHOD FOR PRODUCING ISOBUTENE FROM 3-METHYLCROTONYL-COA | US15511676 | 2015-09-16 | US20170260548A1 | 2017-09-14 | Philippe Marlière; Mathieu Allard |
| Described is a method for the production of isobutene from 3-methylcrotonyl-CoA comprising the steps of:(a) enzymatically converting 3-methylcrotonyl-CoA into 3-methylbutyric acid; and (b) further enzymatically converting the thus produced 3-methylbutyric acid into isobutene. The conversion of 3-methylcrotonyl-CoA into 3-methylbutyric acid can be achieved by first enzymatically converting 3-methylcrotonyl-CoA into 3-methylbutyryl-CoA and further enzymatically converting the thus produced 3-methylbutyryl-CoA into 3-methylbutyric acid. Alternatively, the conversion of 3-methylcrotonyl-CoA into 3-methylbutyric acid can be achieved by first enzymatically converting 3-methylcrotonyl-CoA into 3-methylcrotonic acid and then further enzymatically converting the thus produced 3-methylcrotonic acid into 3-methylbutyric acid. | ||||||
| 6 | Yeast strains autonomously producing steroids | US10470673 | 2002-01-29 | US07670829B2 | 2010-03-02 | Roberto Spagnoli; Tilman Achstetter; Gilles Cauet; Eric Degryse; Bruno Dumas; Denis Pompon; Jacques Winter |
| The present invention relates to genetically modified yeast strains autonomously producing, from a simple carbon source, steroids. The invention also relates to a method for producing steroids from such yeast strains. | ||||||
| 7 | Process for oxidation of steroids and genetically engineered cells used therein | US10462128 | 2003-06-16 | US20040067579A1 | 2004-04-08 | Herman Slijkhuis; Eric Bastiaan Smaal; Gerardus Cornelis Maria Selten |
| An expression cassette, operable in a recombinant host, comprising a heterologous DNA coding sequence encoding a protein, which is functional, alone or in cooperation with one or more additional proteins, of catalyzing an oxidation step in the biological pathway for conversion of cholesterol into hydrocortisone, which step is selected from the group consisting of: the conversion of cholesterol to pregnenolone; the conversion of pregnenolone to progesterone; the conversion of progesterone to 17null-hydroxy-progesterone; the conversion of 17null-hydroxyprogesterone to cortexolone; the conversion of cortexolone to hydrocortisone, and the corresponding control sequences effective in said host. | ||||||
| 8 | Process for the biochemical oxidation of steroids and genetically engineered cells to be used therefor | EP89201173.5 | 1989-05-08 | EP0340878B1 | 2001-05-16 | Slijkhuis, Harmen; Selten, Gerardus Cornelis Maria; Smaal, Erik Bastiaan |
| 9 | Yeast strain which autonomously produce the steroid | JP2002561663 | 2002-01-29 | JP2004528827A | 2004-09-24 | エリク・デグリーズ; ジール・カウエ; ジャック・ウィンテ; ティルマン・アハシュテッター; デニ・ポンポン; ブルーノ・デュマ; ロベルト・スパニョーリ |
| 本発明は、単純な炭素源からステロイドを自律的に産生する遺伝的に改変された酵母株に関する。 本発明はまたこの酵母株からステロイドを生成する方法に関する。 | ||||||
| 10 | JPH03501445A - | JP51062189 | 1989-09-25 | JPH03501445A | 1991-04-04 | |
| 11 | Biochemical oxidation methods and genetically engineered cells used for the steroid | JP50570789 | 1989-05-08 | JP2963711B2 | 1999-10-18 | SUREIKUHAISU HERUMAN; SERUTEN HERARUDEYUSU KORUNERISU MARIA; SUMAARU ERITSUKU BASUCHIAAN |
| 12 | JPH04500303A - | JP50570789 | 1989-05-08 | JPH04500303A | 1992-01-23 | |
| 13 | Yeast strain which autonomously produce the steroid | JP2002561663 | 2002-01-29 | JP4620932B2 | 2011-01-26 | エリク・デグリーズ; ジール・カウエ; ジャック・ウィンテ; ティルマン・アハシュテッター; デニ・ポンポン; ブルーノ・デュマ; ロベルト・スパニョーリ |
| 14 | Biochemical oxidation of steroid | JP7855699 | 1999-03-23 | JPH11308991A | 1999-11-09 | SLIJKHUIS HERMAN; SELTEN GERARDUS CORNELIS MARIA; SMAAL ERIC BASTIAAN |
| PROBLEM TO BE SOLVED: To economically obtain a steroid useful as a medicine or the like by incubating a sterol compound in the presence of protein(s) produced by a specific process under such conditions as to enable a steroid to be oxidized and the resulting oxidation product to be accumulated in the culture fluid. SOLUTION: This steroid is obtained by incubating a sterol compound in the presence of protein(s) under such conditions as to enable a steroid to be oxidized and the resulting oxidation product to be accumulated in the culture fluid, followed by recovering the oxidation product. The above reaction is an in vitro selective biochemical oxidation, and the above-mentioned protein(s) is produced by the following procedure: a bovine adrenocortical cDNA library is screened with a probe consisting of a partial sequence of bovine cytochrome P 450 steroid 21-hydroxylase (P 450C21) gene, the P 450C21 gene thus obtained is integrated into a vector and expressed in host cells. By using the above protein(s) in the aforementioned process, hydrocortisone or the like useful as a medicine can be economically obtained by several stage oxidation of a sterol compound. COPYRIGHT: (C)1999,JPO | ||||||
| 15 | Yeast strains autonomously producing steroids | US12683642 | 2010-01-07 | US07977065B2 | 2011-07-12 | Roberto Spagnoli; Tilman Achstetter; Gilles Cauet; Eric Degryse; Bruno Dumas; Denis Pompon; Jacques Winter |
| The present invention relates to genetically modified yeast strains autonomously producing, from a simple carbon source, steroids. The invention also relates to a method for producing steroids from such yeast strains. | ||||||
| 16 | YEAST STRAINS AUTONOMOUSLY PRODUCING STEROIDS | US12683642 | 2010-01-07 | US20100112634A1 | 2010-05-06 | Roberto SPAGNOLI; Tilman ACHSTETTER; Gilles CAUET; Eric DEGRYSE; Bruno DUMAS; Denis POMPON; Jacques WINTER |
| The present invention relates to genetically modified yeast strains autonomously producing, from a simple carbon source, steroids. The invention also relates to a method for producing steroids from such yeast strains. | ||||||
| 17 | Yeast strains autonomously producing steroids | US10470673 | 2004-02-05 | US20040137556A1 | 2004-07-15 | Roberto Spagnoli; Tilman Achstetter; Gilles Cauet; Eric Degryse; Bruno Dumas; Denis Pompon; Jacques Winter |
| The present invention relates to genetically modified yeast strains autonomously producing, from a simple carbon source, steroids. The invention also relates to a method for producing steroids from such yeast strains. | ||||||
| 18 | Process for oxidation of steroids and genetically engineered cells used therein | US10084814 | 2002-02-26 | US20030108982A1 | 2003-06-12 | Herman Slijkhuis; Eric Bastiaan Smaal; Gerardus Cornelis Maria Selten |
| An expression cassette, operable in a recombinant host, comprising a heterologous DNA coding sequence encoding a protein, which is functional, alone or in cooperation with one or more additional proteins, of catalyzing an oxidation step in the biological pathway for conversion of cholesterol into hydrocortisone, which step is selected from the group consisting a of: the conversion of cholesterol to pregnenolone; the conversion of pregnenolone to progesterone; the conversion of progesterone to 17null-hydroxy-progesterone; the conversion of 17null-hydroxyprogesterone to cortexolone; the conversion of cortexolone to hydrocortisone, and the corresponding control sequences effective in said host. | ||||||
| 19 | Process for oxidation of steroids and genetically engineered cells used therein | US09099011 | 1998-06-17 | US06171836B2 | 2001-01-09 | Herman Slijkhuis; Eric Bastiaan Smaal; Gerardus Cornelis Maria Selten |
| An expression cassette, operable in a recombinant host, comprising a heterologous DNA coding sequence encoding a protein, which is functional, alone or in cooperation with one or more additional proteins, of catalyzing an oxidation step in the biological pathway for conversion of cholesterol into hydrocortisone, which step is selected from the group consisting of: the conversion of cholesterol to pregnenolone; the conversion of pregnenolone to progesterone; the conversion of progesterone to 17&agr;-hydroxyprogesterone; the conversion of 17&agr;-hydroxyprogesterone to cortexolone; the conversion of cortexolone to hydrocortisone, and the corresponding control sequences effective in said host. | ||||||
| 20 | 자율적으로 스테로이드를 생산하는 효모 균주 | KR1020037010173 | 2002-01-29 | KR1020030082580A | 2003-10-22 | 스파크놀리로베르토; 아슈테터틸만; 코에트길레; 데그리세에릭; 두마스부르노; 폼퐁당스; 윈테쟈끄 |
| 본 발명은 단순한 탄소원으로부터 스테로이드를 자율적으로 생산하는 유전학적으로 변형된 효모 균주에 관한 것이다. 본 발명은 또한 당해 효모 균주로부터 스테로이드를 생산하는 방법에 관한 것이다. | ||||||
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