| 序号 | 专利名 | 申请号 | 申请日 | 公开（公告）号 | 公开（公告）日 | 发明人 |
|---|---|---|---|---|---|---|
| 1 | 用于生物合成化合物的方法、试剂和细胞 | CN201580043260.X | 2015-06-16 | CN106795536A | 2017-05-31 | A.L.博特斯; A.V.E.康拉迪; R.哈杜什 |
| 本文献描述了使用脂肪酸O‑甲基转移酶，醇O‑乙酰转移酶，和单加氧酶的一种或多种产生5‑羟基戊酸甲酯和戊酸戊酯的生化途径，以及表达此类外源酶的一种或多种的重组宿主。可将5‑羟基戊酸甲酯和戊酸戊酯酶促转化为戊二酸，5‑氨基戊酸，5‑羟基戊酸，尸胺，或1,5‑戊二醇。 | ||||||
| 2 | CYP101酶重组载体及构建方法、CYP101酶高效表达纯化方法 | CN201610938377.0 | 2016-10-25 | CN106497956A | 2017-03-15 | 黄娟; 徐沁; 洪亮 |
| 本发明涉及CYP101酶重组载体及构建方法、CYP101酶高效表达纯化方法，属于生物技术领域。本发明中构建了PET28a-CYP101的高效表达载体，并将其转化于BL21 plysS菌株中，诱导表达后，利用简单两步纯化方法：即亲和层析和离子交换层析，得到纯度大于95％的高纯酶。与现有技术相比，本发明对CYP101生产简单高效，成本低廉，纯化方便，每升培养基可获得23mg纯度95％以上的目的蛋白。本发明可用于CYP101酶的表达纯化，极大地提高了实验室生产的效率，同时极大的降低了实验成本。对于大规模生产纯化CYP101酶具有很好的应用前景。 | ||||||
| 3 | 用于生物合成化合物的方法、试剂和细胞 | CN201580043843.2 | 2015-06-16 | CN106795537A | 2017-05-31 | A.L.博特斯; A.V.E.康拉迪; R.哈杜什 |
| 本文件描述了使用脂肪酸O‑甲基转移酶，醇O‑乙酰转移酶，和单加氧酶的一种或多种产生7‑羟基庚酸甲酯和庚酸庚酯的生化途径，以及表达此类外源酶的一种或多种的重组宿主。可将7‑羟基庚酸甲酯和庚酸庚酯酶促转化为庚二酸，7‑氨基庚酸，7‑羟基庚酸，七亚甲基二胺，或1,7‑庚二醇。 | ||||||
| 4 | METHODS OF PRODUCING 6-CARBON CHEMICALS VIA CoA-DEPENDENT CARBON CHAIN ELONGATION ASSOCIATED WITH CARBON STORAGE | US14666085 | 2015-03-23 | US20150267211A1 | 2015-09-24 | Adriana Leonora Botes; Alex Van Eck Conradie |
| This document describes biochemical pathways for producing adipic acid, caprolactam, 6-aminohexanoic acid, 6-hydroxyhexanoic acid, hexamethylenediamine or 1,6-hexanediol by forming two terminal functional groups, comprised of carboxyl, amine or hydroxyl groups, in a C6; backbone substrate. These pathways, metabolic engineering and cultivation strategies described herein rely on CoA-dependent elongation enzymes or analogues enzymes associated with the carbon storage pathways from polyhydroxyalkanoate accumulating bacteria. | ||||||
| 5 | Methods, reagents and cells for biosynthesizing compounds | US14741259 | 2015-06-16 | US09777302B2 | 2017-10-03 | Alex Van Eck Conradie; Adriana Leonora Botes; Ramdane Haddouche |
| This document describes biochemical pathways for producing 5-hydroxypentanoate methyl ester and pentanoic acid pentyl ester using one or more of a fatty acid O-methyltransferase, an alcohol O-acetyltransferase, and a monooxygenase, as well as recombinant hosts expressing one or more of such exogenous enzymes. 5-hydroxypentanoate methyl esters and pentanoic acid pentyl esters can be enzymatically converted to glutaric acid, 5-aminopentanoate, 5-hydroxypentanoate, cadaverine, or 1,5-pentanediol. | ||||||
| 6 | Methods, reagents and cells for biosynthesizing compounds | US14741194 | 2015-06-16 | US09738914B2 | 2017-08-22 | Alex Van Eck Conradie; Adriana Leonora Botes; Ramdane Haddouche |
| This document describes biochemical pathways for producing 7-hydroxyheptanoate methyl ester and heptanoic acid heptyl ester using one or more of a fatty acid O-methyltransferase, an alcohol O-acetyltransferase, and a monooxygenase, as well as recombinant hosts expressing one or more of such exogenous enzymes. 7-hydroxyheptanoate methyl esters and heptanoic acid heptyl esters can be enzymatically converted to pimelic acid, 7-aminoheptanoate, 7-hydroxyheptanoate, heptamethylenediamine, or 1,7-heptanediol. | ||||||
| 7 | METHODS, REAGENTS AND CELLS FOR BIOSYNTHESIZING COMPOUNDS | US14741194 | 2015-06-16 | US20150361465A1 | 2015-12-17 | Adriana Leonora Botes; Alex Van Eck Conradie; Ramdane Haddouche |
| This document describes biochemical pathways for producing 7-hydroxyheptanoate methyl ester and heptanoic acid heptyl ester using one or more of a fatty acid O-methyltransferase, an alcohol O-acetyltransferase, and a monooxygenase, as well as recombinant hosts expressing one or more of such exogenous enzymes. 7-hydroxyheptanoate methyl esters and heptanoic acid heptyl esters can be enzymatically converted to pimelic acid, 7-aminoheptanoate, 7-hydroxyheptanoate, heptamethylenediamine, or 1,7-heptanediol. | ||||||
| 8 | METHODS, REAGENTS AND CELLS FOR BIOSYNTHESIZING COMPOUNDS | EP15731806.4 | 2015-06-16 | EP3155110A1 | 2017-04-19 | BOTES, Adriana Leonora; CONRADIE, Alex Van Eck; HADDOUCHE, Ramdane |
| This document describes biochemical pathways for producing 7-hydroxyheptanoate methyl ester and heptanoic acid heptyl ester using one or more of a fatty acid O-methyltransferase, an alcohol O-acetyltransferase, and a monooxygenase, as well as recombinant hosts expressing one or more of such exogenous enzymes. 7-hydroxyheptanoate methyl esters and heptanoic acid heptyl esters can be enzymatically converted to pimelic acid, 7-aminoheptanoate, 7-hydroxyheptanoate, heptamethylenediamine, or 1,7-heptanediol. | ||||||
| 9 | METHODS, REAGENTS AND CELLS FOR BIOSYNTHESIZING COMPOUNDS | EP15731478.2 | 2015-06-16 | EP3155108A1 | 2017-04-19 | BOTES, Adriana Leonora; CONRADIE, Alex Van Eck; HADDOUCHE, Ramdane |
| This document describes biochemical pathways for producing 7-hydroxyheptanoate methyl ester and heptanoic acid heptyl ester using one or more of a fatty acid O-methyltransferase, an alcohol O-acetyltransferase, and a monooxygenase, as well as recombinant hosts expressing one or more of such exogenous enzymes. 7-hydroxyheptanoate methyl esters and heptanoic acid heptyl esters can be enzymatically converted to pimelic acid, 7-aminoheptanoate, 7-hydroxyheptanoate, heptamethylenediamine, or 1,7-heptanediol. | ||||||
| 10 | METHODS, REAGENTS AND CELLS FOR BIOSYNTHESIZING COMPOUNDS | US15642269 | 2017-07-05 | US20180002729A1 | 2018-01-04 | Alex Van Eck Conradie; Adriana Leonora Botes; Ramdane Haddouche |
| This document describes biochemical pathways for producing 7-hydroxyheptanoate methyl ester and heptanoic acid heptyl ester using one or more of a fatty acid O-methyltransferase, an alcohol O-acetyltransferase, and a monooxygenase, as well as recombinant hosts expressing one or more of such exogenous enzymes. 7-hydroxyheptanoate methyl esters and heptanoic acid heptyl esters can be enzymatically converted to pimelic acid, 7-aminoheptanoate, 7-hydroxyheptanoate, heptamethylenediamine, or 1,7-heptanediol. | ||||||
| 11 | Methods, reagents and cells for biosynthesizing compounds | US14741228 | 2015-06-16 | US09816117B2 | 2017-11-14 | Alex Van Eck Conradie; Adriana Leonora Botes; Ramdane Haddouche |
| This document describes biochemical pathways for producing 6-hydroxyhexanoate methyl ester and hexanoic acid hexyl ester using one or more of a fatty acid O-methyltransferase, an alcohol O-acetyltransferase and a monooxygenase, as well as recombinant hosts expressing one or more of such enzymes. 6-hydroxyhexanoate methyl esters and hexanoic acid hexyl ester can be enzymatically converted to adipic acid, adipate semialdehyde, 6-aminohexanoate, 6-hydroxyhexanoate, hexamethylenediamine, and 1,6-hexanediol. | ||||||
| 12 | BACTERIAL CYTOCHROME P450 PROTEIN VARIANT AND METHOD OF REDUCING CONCENTRATION OF FLUORINATED METHANE IN SAMPLE USING THE SAME | US15372327 | 2016-12-07 | US20170159030A1 | 2017-06-08 | Yukyung Jung; Dongsik Yang; Jinhwan Park; Taeyong Kim; Changduk Kang; Anirban Bhaduri; Tadi Venkata Siva Kumar |
| Provided is a recombinant microorganism including an exogenous gene encoding a bacterial cytochrome P450 protein or a variant thereof, a composition including the recombinant P450 protein or the variant thereof, which is used for removing CHnF4-n (n is an integer of 0 to 3) in a sample, and a method of reducing a concentration of CHnF4-n in the sample. | ||||||
| 13 | METHODS, REAGENTS AND CELLS FOR BIOSYNTHESIZING COMPOUNDS | US14741259 | 2015-06-16 | US20150361467A1 | 2015-12-17 | Adriana Leonora Botes; Alex Van Eck Conradie; Ramdane Haddouche |
| This document describes biochemical pathways for producing 5-hydroxypentanoate methyl ester and pentanoic acid pentyl ester using one or more of a fatty acid O-methyltransferase, an alcohol O-acetyltransferase, and a monooxygenase, as well as recombinant hosts expressing one or more of such exogenous enzymes. 5-hydroxypentanoate methyl esters and pentanoic acid pentyl esters can be enzymatically converted to glutaric acid, 5-aminopentanoate, 5-hydroxypentanoate, cadaverine, or 1,5-pentanediol. | ||||||
| 14 | METHODS, REAGENTS AND CELLS FOR BIOSYNTHESIZING COMPOUNDS | US14741228 | 2015-06-16 | US20150361466A1 | 2015-12-17 | Adriana Leonora Botes; Alex Van Eck Conradie; Ramdane Haddouche |
| This document describes biochemical pathways for producing 6-hydroxyhexanoate methyl ester and hexanoic acid hexyl ester using one or more of a fatty acid O-methyltransferase, an alcohol O-acetyltransferase and a monooxygenase, as well as recombinant hosts expressing one or more of such enzymes. 6-hydroxyhexanoate methyl esters and hexanoic acid hexyl ester can be enzymatically converted to adipic acid, adipate semialdehyde, 6-aminohexanoate, 6-hydroxyhexanoate, hexamethylenediamine, and 1,6-hexanediol. | ||||||
| 15 | Methods of producing 6-carbon chemicals via CoA-dependent carbon chain elongation associated with carbon storage | US14106124 | 2013-12-13 | US09102960B2 | 2015-08-11 | Adriana Leonora Botes; Alex Van Eck Conradie |
| This document describes biochemical pathways for producing adipic acid, caprolactam, 6-aminohexanoic acid, 6-hydroxyhexanoic acid, hexamethylenediamine or 1,6-hexanediol by forming two terminal functional groups, comprised of carboxyl, amine or hydroxyl groups, in a C6 aliphatic backbone substrate. These pathways, metabolic engineering and cultivation strategies described herein rely on CoA-dependent elongation enzymes or analogs enzymes associated with the carbon storage pathways from polyhydroxyalkanoate accumulating bacteria. | ||||||
| 16 | METHODS OF PRODUCING 6-CARBON CHEMICALS VIA CoA-DEPENDENT CARBON CHAIN ELONGATION ASSOCIATED WITH CARBON STORAGE | US14106124 | 2013-12-13 | US20140186902A1 | 2014-07-03 | Adriana Leonora Botes; Alex Van Eck Conradie |
| This document describes biochemical pathways for producing adipic acid, caprolactam, 6-aminohexanoic acid, 6-hydroxyhexanoic acid, hexamethylenediamine or 1,6-hexanediol by forming two terminal functional groups, comprised of carboxyl, amine or hydroxyl groups, in a C6 aliphatic backbone substrate. These pathways, metabolic engineering and cultivation strategies described herein rely on CoA-dependent elongation enzymes or analogues enzymes associated with the carbon storage pathways from polyhydroxyalkanoate accumulating bacteria. | ||||||
| 17 | BACTERIAL CYTOCHROME P450 PROTEIN VARIANT AND METHOD OF REDUCING CONCENTRATION OF FLUORINATED METHANE IN SAMPLE USING THE SAME | EP16202662.9 | 2016-12-07 | EP3178922A1 | 2017-06-14 | JUNG, Yukyung; YANG, Dongsik; PARK, Jinhwan; KIM, Taeyong; KANG, Changduk; BHADURI, Anirban; SIVA KUMAR, Tadi Venkata |
Provided is a recombinant microorganism including an exogenous gene encoding a bacterial cytochrome P450 protein or a variant thereof, a composition including the recombinant P450 protein or the variant thereof, which is used for removing CHnF4-n (n is an integer of 0 to 3) in a sample, and a method of reducing a concentration of CHnF4-n in the sample.
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| 18 | METHODS, REAGENTS AND CELLS FOR BIOSYNTHESIZING COMPOUNDS | EP15731805.6 | 2015-06-16 | EP3155109A1 | 2017-04-19 | BOTES, Adriana Leonora; CONRADIE, Alex Van Eck; HADDOUCHE, Ramdane |
| This document describes biochemical pathways for producing 7-hydroxyheptanoate methyl ester and heptanoic acid heptyl ester using one or more of a fatty acid O-methyltransferase, an alcohol O-acetyltransferase, and a monooxygenase, as well as recombinant hosts expressing one or more of such exogenous enzymes. 7-hydroxyheptanoate methyl esters and heptanoic acid heptyl esters can be enzymatically converted to pimelic acid, 7-aminoheptanoate, 7-hydroxyheptanoate, heptamethylenediamine, or 1,7-heptanediol. | ||||||
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