| 序号 | 专利名 | 申请号 | 申请日 | 公开（公告）号 | 公开（公告）日 | 发明人 |
|---|---|---|---|---|---|---|
| 1 | 用于产生3-羧基黏康酸内酯的基因被破坏的菌株、重组质粒、转化体和方法 | CN200880015737.3 | 2008-05-09 | CN101679963A | 2010-03-24 | 下俊久; 间濑浩平; 片山义博; 政井英司; 福田雅夫; 重原淳孝; 大原诚资; 中村雅哉; 大塚祐一郎 |
| 本发明意欲通过发酵在工业规模上从对苯二甲酸产生3-羧基-顺，顺黏康酸。原儿茶酸4，5-环切割酶的基因被破坏的菌株，在该菌株中，存在于微生物细胞的染色体DNA上的基因已被破坏，所述基因编码氨基酸序列，所述氨基酸序列是(a)由SEQ ID NO：1或3表示的氨基酸序列，或(b)通过一个或多个氨基酸的缺失、置换、添加和/或插入从EQ ID NO：1或3表示的氨基酸序列衍生的氨基酸序列，并且具有原儿茶酸4，5-环切割活性；包含Tph基因和原儿茶酸3，4-双加氧酶基因的重组质粒；通过将上述重组质粒导入上述被破坏的菌株而构建的转化体；和产生3-羧基-顺，顺黏康酸和/或3-羧基黏康酸内酯的方法，其特征在于包括在对苯二甲酸存在的情况下培养上述转化体。 | ||||||
| 2 | Gene-destructing strain, recombinant plasmid, transformant, and method for producing 3-carboxymuconolactone | JP2007126990 | 2007-05-11 | JP2008278823A | 2008-11-20 | SHIMO TOSHIHISA; MASE KOHEI; KATAYAMA YOSHIHIRO; MASAI EIJI; FUKUDA MASAO; SHIGEHARA JUNKO; OHARA SEISHI; NAKAMURA MASAYA; OTSUKA YUICHIRO |
| <P>PROBLEM TO BE SOLVED: To fermentatively produce 3-carboxy-cis,cis-muconic acid from terephthalic acid in an industrial scale. <P>SOLUTION: Disclosed are a protocatechuic acid 4,5-ring cleaving enzyme gene-destructing strain, wherein a gene encoding an amino acid sequence which is (a) a specific amino acid sequence or (b) an amino acid sequence in which one or more amino acids are deleted, substituted, added and/or inserted in the specific amino acid sequence and in which a gene for encoding protocatechuic acid 4,5-ring cleaving activity is destructed on the chromosomal DNA of a bacterial cell; a recombinant plasmid including a Tph gene and a protocatechuic acid 3,4-dioxygenase gene; and a method for producing 3-carboxy-cis,cis-muconic acid and/or 3-carboxymuconolactone for culturing the transformant in the presence of terephthalic acid. <P>COPYRIGHT: (C)2009,JPO&INPIT | ||||||
| 3 | GENE-DISRUPTED STRAIN, RECOMBINANT PLASMID, TRANSFORMANT AND METHOD OF PRODUCING 3-CARBOXYMUCONOLACTONE | EP08752842.8 | 2008-05-09 | EP2147973A1 | 2010-01-27 | SHIMO, Toshihisa; MASE, Kohei; KATAYAMA, Yoshihiro; MASAI, Eiji; FUKUDA, Masao; SHIGEHARA, Kiyotaka; OHARA, Seiji; NAKAMURA, Masaya; OTSUKA, Yuichiro |
Industrial-scale fermentative production of 3-carboxy-cis,cis-muconic acid from terephthalic acid. |
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| 4 | GENE-DISRUPTED STRAIN, RECOMBINANT PLASMID, TRANSFORMANT AND METHOD OF PRODUCING 3-CARBOXYMUCONOLACTONE | EP08752842 | 2008-05-09 | EP2147973A4 | 2010-06-09 | SHIMO TOSHIHISA; MASE KOHEI; KATAYAMA YOSHIHIRO; MASAI EIJI; FUKUDA MASAO; SHIGEHARA KIYOTAKA; OHARA SEIJI; NAKAMURA MASAYA; OTSUKA YUICHIRO |
| 5 | Method for Producing Objective Substance | US16392756 | 2019-04-24 | US20190249205A1 | 2019-08-15 | Benjamin Mijts; Christine Roche; Peter Kelly; Sayaka Asari; Keita Fukui; Miku Toyazaki |
| A method for producing an objective substance such as vanillin and vanillic acid is provided. An objective substance is produced from a carbon source or a precursor of the objective substance by using a microorganism having an objective substance-producing ability, which microorganism has been modified so that the activity of an L-cysteine biosynthesis enzyme is increased. | ||||||
| 6 | CONVERSION OF BIOMASS TO USEFUL INTERMEDIATES | US15467761 | 2017-03-23 | US20170275655A1 | 2017-09-28 | Gregg Tyler BECKHAM; Christopher W. JOHNSON; Derek R. VARDON |
| An aspect of the present disclosure is a microbial cell that includes a genetic modification resulting in the expression of a deficient form of an endogenous dioxygenase, and a gene encoding an exogenous dioxygenase and a promoter sequence, where the endogenous dioxygenase includes PcaH and PcaG, the exogenous dioxygenase includes LigA and LigB, the microbial cell is capable of growth utilizing at least one of a cellulose decomposition molecule or a lignin decomposition molecule, and the microbial cell is capable of producing 2-hydroxy-2H-pyran-4,6-dicarboxylic acid. | ||||||
| 7 | GENE-DISRUPTED STRAIN, RECOMBINANT PLASMIDS, TRANSFORMANTS AND PROCESS FOR PRODUCTION OF 3-CARBOXYMUCONOLACTONE | US12599787 | 2008-05-09 | US20100209978A1 | 2010-08-19 | Toshihisa Shimo; Kohei Mase; Yoshihiro Katayama; Eiji Masai; Masao Fukuda; Kiyotaka Shigehara; Seiji Ohara; Masaya Nakamura; Yuichiro Otsuka |
| Industrial-scale fermentative production of 3-carboxy-cis,cis-muconic acid from terephthalic acid. Also, a protocatechuate 4,5-ring-cleaving enzyme gene-disrupted strain in which the gene coding for (a) the amino acid sequence set forth in SEQ ID NO: 1 or 3, or (b) the amino acid sequence set forth in SEQ ID NO: 1 or 3 which has a deletion, substitution, addition and/or insertion of one or more amino acids and exhibits protocatechuate 4,5-ring cleavage activity, present in the chromosomal DNA of microbial cells, has been disrupted; recombinant plasmids comprising the Tph gene and protocatechuate 3,4-dioxygenase gene; transformants obtained by introducing the recombinant plasmids into the disrupted strain; and a process for production of 3-carboxy-cis,cis-muconic acid and/or 3-carboxymuconolactone characterized by culturing the transformants in the presence of terephthalic acid. | ||||||
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