序号 专利名 申请号 申请日 公开(公告)号 公开(公告)日 发明人
1 L-Lysine .alpha.-oxidase US15179 1979-02-26 US4234691A 1980-11-18 Hitoshi Kusakabe; Kenjiro Kodama; Yuichiro Midorikawa; Akira Kuninaka; Haruo Misono; Kenji Soda
An L-lysine .alpha.-oxidase, that is, a novel L-amino acid oxidase having very high substrate-specificity to L-lysine is produced by culturing a specific microorganism belonging to Trichoderma in a medium.
2 Assay of L-lysine US15177 1979-02-26 US4224407A 1980-09-23 Hitoshi Kusakabe; Akira Kuninaka; Kenji Soda
L-lysine contained in a sample can be efficiently determined by using the L-lysine .alpha.-oxidase. The preferred enzyme is an L-lysine .alpha.-oxidase, that is, a novel L-amino acid oxidase having very high substrate-specificity to L-lysine is produced by culturing a specific microorganism belonging to Trichoderma in a medium.
3 Estimation method of l-lysine JP11586778 1978-09-22 JPS5543409A 1980-03-27 KUSAKABE HITOSHI; KUNINAKA AKIRA; SOUDA KENJI
PURPOSE: To estimate L-lysine remarkably with high sensitivity and simplicity by employing the enzyme reaction of L-lysine α-oxidase. CONSTITUTION: L-lysine α-oxidase which the molds pertaining to trichodermer group produce dissolves L-rising under the presence of oxygen as represented by formula, αamino group of L-lysine is subjected to oxidation deamination to produce NH 3, H 2O 2, α-keto -ε- aminocaproic acid or Δ'-pyperizine-2-carboxylic acid. Accordingly, from the consuming amount of the products thereof or the consumed amount of O 2 in the reaction solution, L-lysine is estimated. COPYRIGHT: (C)1980,JPO&Japio
4 JPS5632919B2 - JP11586778 1978-09-22 JPS5632919B2 1981-07-30
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