子分类：
| 序号 | 专利名 | 申请号 | 申请日 | 公开（公告）号 | 公开（公告）日 | 发明人 |
|---|---|---|---|---|---|---|
| 41 | ELECTROMANIPULATION OF PROTEINS USING NANOSECOND PULSED ELECTRIC FIELDS | US15507820 | 2015-09-02 | US20170304002A1 | 2017-10-26 | Stephen J. BEEBE; Karl H. SCHOENBACH |
| The present disclosure describes methods for intracellular electromanipulation of proteins using nanosecond pulsed electric fields (nsPEFs). The nsPEFs have effects on proteins in addition to permeabilizing cellular membranes. The nsPEFs induce a Ca2+-dependent dissipation of the mitochondria membrane potential (ΔΨm), which is enhanced when high frequency components are present in fast rise-fall waveforms. Ca2+ is shown to have little or no effect on propidium iodide uptake as a measure of plasma membrane poration and consequently intracellular membranes. Since Ca2+-regulated events are mediated by proteins, actions of nsPEFs on proteins that regulate and/or affect the mitochondria membrane potential are possible. Given that nsPEF-induced dissipation of ΔΨm was more effective when high frequency components were present in fast rise time waveforms, the effects on proteins are due to these high frequency components. These results present direct evidence that nsPEFs affect proteins and their functions by affecting their structure. | ||||||
| 42 | MESENCHYMAL-LIKE STEM CELLS DERIVED FROM HUMAN EMBRYONIC STEM CELLS, METHODS AND USES THEREOF | US15635022 | 2017-06-27 | US20170290864A1 | 2017-10-12 | Xiaofang WANG; Ren-He XU |
| The disclosure provided herein relates generally to mesenchymal-like stem cells “hES-T-MiSC” or “T-MSC” and the method of producing the stem cells. The method comprises culturing embryonic stem cells under conditions that the embryonic stem cells develop through an intermediate differentiation of trophoblasts, and culturing the differentiated trophoblasts to hES-T-MSC or T-MSC, T-MSC derived cells and cell lineages “T-MSC-DL” are also described. Disclosed also herein are solutions and pharmaceutical compositions comprising the T-MSC and/or T-MSC-DL, methods of making the T-MSC and T-MSC-DL, and methods of using the T-MSC and T-MSC-DL for treatment and prevention of diseases, specifically, T-MSC and T-MSC-DL are used as immunosuppressive agents to treat multiple sclerosis and autoimmune diseases. | ||||||
| 43 | Magnetic extracellular matrix | US14404469 | 2013-06-11 | US09764030B2 | 2017-09-19 | Glauco R. Souza |
| Methods of making and using a magnetic ECM are disclosed. The ECM comprises positively and negatively charged nanoparticles, wherein one of said nanoparticles contains a magnetically responsive element. When the magnetic ECM is seeded with cells, the cells will be magnetized and can be levitated for 3-D cell culture. | ||||||
| 44 | MAGNETIC DIRECTED ALIGNMENT OF STEM CELL SCAFFOLDS FOR REGENERATION | US15315247 | 2015-06-03 | US20170106088A1 | 2017-04-20 | Dong LIU; Qilin CAO |
| The present disclosure relates to liposomal delivery vehicles comprising magnetic particles and their use in modifying stem cells for delivery to target sites such as neuronal tissues. | ||||||
| 45 | Electrokinetic confinement of neurite growth for dynamically configurable neural networks | US14154823 | 2014-01-14 | US09605254B2 | 2017-03-28 | Joel Voldman; Thibault Honegger; David Peyrade |
| Systems and methods for altering neurite growth are generally described. In some embodiments, a system may include a neuron comprising a neurite and electrodes able to generate a physical guidance cue. The physical guidance cue may be used to alter the growth of the neurite and may be temporally and spatially dynamic, such that neurite growth may be altered in a spatial and/or temporal manner. Dynamic control of neurite growth may be used to form directional neural connections, intersections, and/or overlaps. | ||||||
| 46 | System and Method For A Piezoelectric Scaffold For Nerve Growth and Repair | US15134639 | 2016-04-21 | US20170051251A1 | 2017-02-23 | Treena Arinzeh; George Collins; Yee-Shuan Lee |
| Provided is an electroactive structure for growing isolated differentiable cells comprising a three dimensional matrix of fibers formed of a biocompatible synthetic piezoelectric polymeric material, wherein the matrix of fibers is seeded with the isolated differentiable cells and forms a supporting scaffold for growing the isolated differentiable cells, and wherein the matrix of fibers stimulates differentiation of the isolated differentiable cells into a mature cell phenotype on the structure. | ||||||
| 47 | SYSTEM AND METHOD FOR MAGNETIC SELF-ASSEMBLY | US15121635 | 2015-02-25 | US20160362655A1 | 2016-12-15 | Utkan Demirci; Savas Tasoglu |
| In one aspect, the present disclosure provides a method for self-assembly of magnetic building blocks, including distributing a plurality of building blocks in a liquid medium, each of the plurality of building blocks having a plurality of stable radicals, establishing a magnetic field interacting with at least a portion of the plurality of building blocks, guiding with the magnetic field the portion of the plurality of building blocks from a first location in the liquid medium to a second location in the liquid medium, assembling into a first construct the portion of the plurality of building blocks proximate the second location, and treating the first construct with at least one antioxidant to neutralize at least in part the plurality of stable radicals. | ||||||
| 48 | Ferromagnetic cell and tissue culture microcarriers | US14459668 | 2014-08-14 | US09476025B2 | 2016-10-25 | Jeanne L. Becker |
| A porous, collagen coated, ferromagnetic cell culture microcarrier, which is suitable for in vitro cell and tissue culture and which facilitates 3D multicellular construct generation. Also provided is a method for creating batches of microcarriers which have inserted within them magnetite (Fe3O4) in the presence of collagen, thus creating a microcarrier which becomes magnetic in nature when placed in a the presence of a magnetic field and which facilitates cellular adherence (via the collagen coating) for 3D construct development. | ||||||
| 49 | Method and Apparatus for Reprogramming Living Cells | US15019053 | 2016-02-09 | US20160230167A1 | 2016-08-11 | Karsten KOENIG; Aisada UCHUGONOVA |
| A method and an apparatus for reprogramming living cells without using viruses. In that method a cocktail comprising at least two transcription factors and a microRNA is transfected into the interior of at least one cell in order to convert this cell into iPS cells or into another type of cell, by storing the cells to be converted in an aqueous environment of the cocktail without viral carriers and focusing a femtosecond laser in a laser scanning microscope with a numerical aperture between 0.9 and 1.5 on a cell membrane of the cell to be reprogrammed and controlling the position of the focus. The exposure period and laser power for the optical treatment of the cell such that the focus depending on the pulse repetition frequency with an output between 7 mW and 100 mW generates a transient small-pore hole with a size up to 500 nm. | ||||||
| 50 | CARDIAC TISSUE CONSTRUCTS AND METHODS OF FABRICATION THEREOF | US14650227 | 2013-12-06 | US20150313704A1 | 2015-11-05 | Nimalan Thavandiran; Milica Radisic; Peter Zandstra |
| Methods and devices are provided for the formation of cardiac tissue constructs. In some embodiments, methods are provided for forming cardiac tissue constructs that including cardiomyocytes, non-myocytes, and extracellular matrix, and which exhibit properties associated with healthy cardiac tissue. In some embodiments, microfabrication platforms are provided to support the transmission of dynamic electromechanical forces, such that the cardiac microtissue constructs may be formed mimicking the basic microenvironment found in the heart. The microfabrication platform may include retaining features for stabilizing the position of the microtissue construct during its formation, and the microfabrication platform may include a ramped support configured to produce tissue constructs having a ring geometry. In some embodiments, the microfabrication platform may be configured to for the application of point electrical stimulation, and/or to amplify the transduction of force into a visible displacement. | ||||||
| 51 | Electroactive Scaffold | US14685204 | 2015-04-13 | US20150218510A1 | 2015-08-06 | Lisa A. Scott Carnell; Emilie J. Siochi; Nancy M. Holloway; Kam W. Leong; Karina Kulangara |
| A method of manufacturing and/or using a scaffold assembly for stem cell culture and tissue engineering applications is disclosed. The scaffold at least partially mimics a native biological environment by providing biochemical, topographical, mechanical and electrical cues by using an electroactive material. The assembly includes at least one layer of substantially aligned, electrospun polymer fiber having an operative connection for individual voltage application. A method of cell tissue engineering and/or stem cell differentiation that uses the assembly seeded with a sample of cells suspended in cell culture media, incubates and applies voltage to one or more layers, and thus produces cells and/or a tissue construct. In another aspect, the invention provides a method of manufacturing the assembly including the steps of providing a first pre-electroded substrate surface; electrospinning a first substantially aligned polymer fiber layer onto the first surface; providing a second pre-electroded substrate surface; electrospinning a second substantially aligned polymer fiber layer onto the second surface; and, retaining together the layered surfaces with a clamp and/or an adhesive compound. | ||||||
| 52 | Aligned and electrospun piezoelectric polymer fiber assembly and scaffold | US12969076 | 2010-12-15 | US09005604B2 | 2015-04-14 | Lisa A. Scott-Carnell; Emilie J. Siochi; Nancy M. Holloway; Kam W. Leong; Karina Kulangara |
| A scaffold assembly and related methods of manufacturing and/or using the scaffold for stem cell culture and tissue engineering applications are disclosed which at least partially mimic a native biological environment by providing biochemical, topographical, mechanical and electrical cues by using an electroactive material. The assembly includes at least one layer of substantially aligned, electrospun polymer fiber having an operative connection for individual voltage application. A method of cell tissue engineering and/or stem cell differentiation uses the assembly seeded with a sample of cells suspended in cell culture media, incubates and applies voltage to one or more layers, and thus produces cells and/or a tissue construct. In another aspect, the invention provides a method of manufacturing the assembly including the steps of providing a first pre-electroded substrate surface; electrospinning a first substantially aligned polymer fiber layer onto the first surface; providing a second pre-electroded substrate surface; electrospinning a second substantially aligned polymer fiber layer onto the second surface; and, retaining together the layered surfaces with a clamp and/or an adhesive compound. | ||||||
| 53 | Alternating electric current directs, enhances, and accelerates mesenchymal stem cell differentiation into either osteoblasts or chondrocytes but not adipocytes | US13630882 | 2012-09-28 | US08945894B2 | 2015-02-03 | Courtney M. Creecy; Rena Bizios |
| A method for directing, enhancing, and accelerating mesenchymal stem cell functions using alternating electric current. Mesenchymal stem cells are preferentially directed to either osteoblast or chondrocyte lineages, but not to the adipocyte lineage. when exposed to alternating electric current. | ||||||
| 54 | METHODS FOR AGGREGATION AND DIFFERENTIATION OF MAGNETIZED STEM CELLS | US14241672 | 2012-09-03 | US20140349330A1 | 2014-11-27 | Delphine Fayol; Nathalie Luciani; Catherine Le Visage; Florence Gazeau; Claire Wilhelm-Hannetel |
| The invention relates to a process which enables optimal aggregation of cells, typically of stem cells, promoting the organisation thereof and advantageously the differentiation thereof, in particular in the context of the formation of a tissue substitute. This process comprises exposing pretreated cells to a magnetic field and makes it possible to obtain large cell aggregates, even prepared in the absence of support matrix and/or of growth factor. The invention also relates to the cell aggregates that can be obtained using such a process and also to the uses thereof as tissue initiators with a view to obtaining a tissue structure of interest in vitro, ex vivo or in vivo. Moreover, it relates to the resulting tissue structures and to the uses thereof in research or in therapy as tissue substitutes. The present application also provides a method which advantageously makes it possible to monitor the development of the tissue of interest in vivo. | ||||||
| 55 | METHOD FOR IN-VITRO TREATMENT OF DIFFERENTIATED OR UNDIFFERENTIATED CELLS BY APPLICATION ELECTROMAGNETIC FIELDS | US14238499 | 2012-08-10 | US20140212943A1 | 2014-07-31 | Salvatore Rinaldi; Vania Fontani |
| A process is described for the treatment of stem cells or differentiated cells by application of radiofrequency electro-magnetic fields, by means of an electromagnetic field generator (10), comprising a power supply (11), at least one antenna (12) adapted to radiate a scattered electromagnetic field (13) of a power less than 100 mW, a modulator (14) associated with said generator (10) and adapted to modulate the emission thereof, and at least one convector electrode (15), adapted to be direct the radiofrequency currents induced by said electromagnetic field (13), and applied in proximity of said stem or differentiated cells. | ||||||
| 56 | Method of cell culture | US13990642 | 2010-12-06 | US08759100B2 | 2014-06-24 | Naoki Yokoyama; Tomonori Akai |
| This invention provides a means for modifying surface properties of a cell culture substrate under specific conditions, to thereby regulate regions to which cells are allowed to adhere or are not allowed to adhere, depending on cell type. This invention relates to a method of cell culture comprising steps of: applying a positive potential to a conductive region of a substrate comprising a base material having a conductive region and a non-cell-adhesive membrane coupled thereto with the aid of silane, so as to separate the non-cell-adhesive membrane from the substrate; and culturing cells in a region from which the non-cell-adhesive membrane has been separated. | ||||||
| 57 | FERROMAGNETIC CELL AND TISSUE CULTURE MICROCARRIERS | US14090655 | 2013-11-26 | US20140087467A1 | 2014-03-27 | JEANNE L. BECKER |
| A porous, collagen coated, ferromagnetic cell culture microcarrier, which is suitable for in vitro cell and tissue culture and which facilitates 3D multicellular construct generation. Also provided is a method for creating batches of microcarriers which have inserted within them magnetite (Fe3O4) in the presence of collagen, thus creating a microcarrier which becomes magnetic in nature when placed in a the presence of a magnetic field and which facilitates cellular adherence (via the collagen coating) for 3D construct development. | ||||||
| 58 | MATERIALS, MONITORING, AND CONTROLLING TISSUE GROWTH USING MAGNETIC NANOPARTICLES | US13386899 | 2011-02-04 | US20120202239A1 | 2012-08-09 | Ezekiel Kruglick |
| Systems and method for releasing a biological factor in a tissue or organ are disclosed. The system includes one or more nanoparticles distributed in the tissue or organ, the nanoparticles including the biological factor; and a magnetic field generator configured to generate a magnetic field at a first frequency and to apply to the tissue or organ the magnetic field at the first frequency thereby causing at least some of the biological factor to be released from each of the nanoparticles into the tissue or organ. | ||||||
| 59 | MATERIALS FOR MAGNETIZING CELLS AND MAGNETIC MANIPULATION | US13393651 | 2010-09-27 | US20120171744A1 | 2012-07-05 | Glauco R. Souza |
| A material comprising positively and negatively charged nanoparticles, wherein one of said nanoparticles contained a magnetically responsive element, are combined with a support molecule, which is a long natural or synthetic molecule or polymer to make a magnetic nanoparticle assembly. When the magnetic nanoparticle assembly is combined with cells, it will magnetize those cells. The magnetized cells can then be washed to remove the magnetic nanoparticle assembly and the magnetized cells manipulated in a magnetic field. | ||||||
| 60 | Method and apparatus for changing one type of cell into another type of cell | US13288801 | 2011-11-03 | US20120129224A1 | 2012-05-24 | Chauncey B. Sayre |
| A method and apparatus converts host cells of a first type into cells of a second type when the host cells are placed in intimate contact with donor cells of the second type. Under predetermined conditions there is transport of a sufficient number of mRNA molecules from the donor cells into the host cells to reprogram the host cells into the second type. The host and donor cells may be subjected to while in intimate contact to a transporting force that enables the mRNA molecules of the donor cells to penetrate an outer membrane wall of host cells without damaging the membrane wall. The transporting force may include an electric field, a magnetic field, or a combined electric field and magnetic field. | ||||||
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