| 序号 | 专利名 | 申请号 | 申请日 | 公开（公告）号 | 公开（公告）日 | 发明人 |
|---|---|---|---|---|---|---|
| 161 | THREE-DIMESIONAL, PREVASCULARIZED, ENGINEERED TISSUE CONSTRUCTS, METHODS OF MAKING AND METHODS OF USING THE TISSUE CONSTRUCTS | EP13758621 | 2013-03-06 | EP2822612A4 | 2015-08-12 | BERRY JOEL L; WICK TIMOTHY M; MURPHY-ULLRICH JOANNE; PENMAN ANDREW D; CAIN ANDREW W |
| 162 | METHOD OF LOADING AND DISTRIBUTING CELLS IN A BIOREACTOR OF A CELL EXPANSION SYSTEM | EP13756956.2 | 2013-08-20 | EP2885393A1 | 2015-06-24 | NANKERVIS, Brian, J. |
| One or more embodiments are described directed to a method and system for loading and distributing cells in a bioreactor of a cell expansion system. Accordingly, embodiments include methods and systems that may provide for adding a plurality of cells to a fluid within a bioreactor of the cell expansion system. A first percentage of the plurality of cells is allowed to settle in the bioreactor and a second percentage of the plurality of cells is allowed to settle outside of the bioreactor. The first percentage of cells is then expanded in the bioreactor. The second percentage of cells is wasted. | ||||||
| 163 | Nucleic acid amplification apparatus and method | EP07001600.1 | 2007-01-25 | EP1813682B1 | 2014-04-02 | Akai, Yasumasa, c/o Sysmex Corporation; Nakabayashi, Kadzuki, c/o Sysmex Corporation; Tanoshima, Eiji, c/o Sysmex Corporation |
| 164 | LARGE VOLUME EX VIVO ELECTROPORATION METHOD | EP04720783.2 | 2004-03-15 | EP1620537A2 | 2006-02-01 | Walters, Richard E.; King, Alan D. |
| An object of the invention is to provide an electroporation method for treating vesicles with exogenous material for insertion of the exogenous material into the vesicles which includes the steps of: a. retaining a suspension of the vesicles and the exogenous material in a treatment volume in a chamber which includes electrodes, wherein the chamber has a geometric factor (cm-1) defined by the quotient of the electrode gap squared (cm2) divided by the chamber volume (cm3), wherein the geometric factor is less than or equal to 0.1 cm-1, wherein the suspension of the vesicles and the exogenous material is in a medium which is adjusted such that the medium has conductivity in a range spanning 0.01 to 1.0 milliSiemens, wherein the suspension is enclosed in the chamber during treatment, and h. treating the suspension enclosed in the chamber with one or more pulsed electric fields. With the method, the treatment volume of the suspension is scalable, and the time of treatment of the vesicles in the chamber is substantially uniform. | ||||||
| 165 | CELL CULTURE VESSEL, AND CULTURE MEDIUM EXCHANGE SYSTEM | EP17738412.0 | 2017-01-11 | EP3404091A1 | 2018-11-21 | KIMURA, Hiroyuki; MINAMI, Tatsuya; MAKARA, Yasunori |
A cell culture container (100) of the present invention includes, in sequence from above in the gravity direction: a culture-medium holding container (1); a cell holding container (2) that holds cells and a culture medium (A) supplied from the culture-medium holding container (1) through a supply port (4) provided in the top surface thereof; a waste-liquid holding container (3) that holds the culture medium (A) discharged from the cell holding container (2); and a discharge mechanism (5) for discharging the culture medium (A) that has exceeded a predetermined level from the cell holding container (2) to the waste-liquid holding container (3). |
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| 166 | DEVICE AND METHOD FOR VITRIFICATION CRYOPRESERVATION OF CELLS OR TISSUES | EP15815607.5 | 2015-06-01 | EP3162883B1 | 2018-10-31 | MATSUZAWA Atsushi; SUSAKI Katsumitsu |
| The present invention aims to provide a device for vitrification cryopreservation enabling easy and reliable vitrification cryopreservation of a cell or tissue. The device for vitrification cryopreservation of a cell or tissue of the present invention includes a support and a vitrification solution absorber including at least an adhesive layer and a vitrification solution absorbing layer in the given order on the support. The device includes a portion with no adhesive layer disposed between the support and a portion of the vitrification solution absorbing layer configured to hold a cell or tissue. | ||||||
| 167 | PLATELET PRODUCTION METHOD USING ROTARY AGITATION CULTURING METHOD | EP16846363.6 | 2016-09-08 | EP3351627A1 | 2018-07-25 | SHIGEMORI, Tomohiro; OKAMOTO, Haruki |
The present invention provides a method for producing platelets, and the method comprises a step of culturing megakaryocytes in a culture solution in a culture vessel, wherein the culture solution is stirred with a stirrer in the culture step. |
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| 168 | FIBER STRUCTURE FOR USE AS CELL SCAFFOLD MATERIAL | EP15855508 | 2015-10-30 | EP3214165A4 | 2018-07-04 | KADOWAKI KOJI; KUGA CHISA; FUJITA MASAKI; TANAHASHI KAZUHIRO; YAMADA SATOSHI; KANEKO TAKAYUKI; TSUCHIKURA HIROSHI |
| The present invention aims to provide a fiber structure which can be used as a cell scaffold material showing improvement in both the cellular adhesiveness and the cell growth capacity, which improvement was achieved by controlling the fiber orientation in the multifilament and the average fiber diameter, which are physical properties. The present invention provides a fiber structure which can be used as a cell scaffold material, which fiber structure comprises a multifilament formed by bundling monofilaments having an average fiber diameter of 1 to 15 µm, wherein each of the monofilaments satisfies the condition of the following Formula 1: (Y/X)×100‰¥50 ... Formula 1 [wherein in Formula 1, X represents the number of monofilaments for which the average crossing angle is investigated, and Y represents the number of monofilaments having an average crossing angle of not more than 25° in X]. | ||||||
| 169 | CELL CULTURE MEDIUM AND CULTURE METHOD USING SAME | EP15846038 | 2015-08-19 | EP3202889A4 | 2018-05-30 | KATO TOMOHISA; KANEMURA YONEHIRO; SHOFUDA TOMOKO; FUKUSUMI HAYATO |
| It is an object of the present invention to provide a cell culture medium capable of enhancing cell growth efficiency without using feeder cells, in particular wherein the cell culture medium does not comprise serum. According to the present invention, a cell culture medium comprising fibrin 5 and Zeta polypeptide is provided. | ||||||
| 170 | SYSTEMS, DEVICES, KITS AND METHODS FOR SEEDING CELLS OR SETS OF MOLECULES IN AN ARRAY ON A SUBSTRATE | EP15869470 | 2015-12-15 | EP3234234A4 | 2018-05-23 | SHEINMAN YEHOSHUA; SHARIVKIN REVITAL; BELENKOVICH MERAV |
| The present disclosure provides systems, devices and methods for seeding cells or sets of molecules on a substrate by utilizing a seeding mesh, to obtain an essentially homogenous patterned seeding of the cells or sets of molecules on the mesh. | ||||||
| 171 | METHOD FOR PRODUCING THREE-DIMENSIONAL CELL AGGREGATES | EP15846123 | 2015-09-29 | EP3202894A4 | 2018-05-23 | YOKOYAMA UTAKO; ISHIKAWA YOSHIHIRO; KANEKO MAKOTO; SAKUMA SHINYA; ARAI FUMIHITO |
| Means which enables preparation of a thick cell aggregate by a simple process without an operation of detaching and stacking of cells is disclosed. The method for preparing a three-dimensional cell aggregate by the present invention comprises: a cell encasing step of placing a cell suspension in a cell container; and a pressure application step of applying pressure to cells in the container. The cell encasing step and the pressure application step may be carried out a plurality of times. By the present invention, a thick, robust cell aggregate can be obtained by a simple operation of applying pressure to a cell suspension or a medium containing cells. Since the method does not require an operation of stacking a plurality of cell sheets, the cells are hardly damaged, and the conditions of the cells can be favorably maintained, so that the cells can be advantageously used as a tissue piece for transplantation. | ||||||
| 172 | DEVICE AND METHOD FOR INDUCING PLURIPOTENT CELLS USING ENERGY | EP15866086 | 2015-12-04 | EP3241896A4 | 2018-05-16 | KIM SOONHAG |
| The present invention relates to a device and a method for inducing pluripotent cells using energy and, more specifically, has an effect of inducing new type pluripotent cells having pluripotent characteristics by applying energy such as ultrasonic waves, lasers or heat treatment to differentiated cells. | ||||||
| 173 | STEM CELL CULTURING METHOD | EP16872965.5 | 2016-12-06 | EP3315603A1 | 2018-05-02 | GLADKOV, Alexei |
[Problem] To provide a stem cell culturing method capable of preventing the proliferation of various stem cells and of culturing only specific types of stem cells. [Solution] A stem cell culturing method in which a second bone marrow aspirate 23 that is an intermediate layer is extracted from a first bone marrow aspirate 19 that is separated into layers; the second bone marrow aspirate 23 is cultured along with a culture medium and first stem cells are fixed on a bottom surface of a first culturing vessel; when a total surface area of the first stem cells reaches a first target ratio with respect to the bottom surface area of the first culturing vessel, the first stem cells are extracted from the first culturing vessel; top layer second stem cells are extracted from the first stem cells that are separated into layers and the second stem cells are cultured along with a culture medium, and the second stem cells are fixed on a bottom surface of a second culturing vessel; and, when a total surface area of the second stem cells reaches a second target ratio with respect to the bottom surface area of the second culturing vessel, the second stem cells are extracted from the second culturing vessel. |
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| 174 | CELL CULTURE DEVICE | EP16786123.6 | 2016-04-15 | EP3290508A1 | 2018-03-07 | KOSEKI, Osamu; SUENAGA, Ryo; KASHIWABARA, Ken |
Provided is a cell culture apparatus that can maintain communication between the inside and outside of the thermostatic chamber through a tube in a state where a door provided with a packing element being closed, and can automatically block communication of the inside and outside of the thermostatic chamber when the tube is removed. On an abutting surface (2a) around the opening part of the thermostatic chamber (3) and the cold storage chamber (4) that abuts against packing elements (3b, 4b) when front doors (3a, 4a) are closed, a communication groove (5) that intercommunicates the inside and outside of the thermostatic chamber (3) by the communication groove (51) when the transfer tube (U40) is removed is provided. The shut-off mechanism (5) is formed of: a guide hole (52) that opens on the abutting surface (2a) across the communication groove (51) and is deeper than the communication groove (51); a shutter member (53) which is inserted into the guide hole (52) and moves between the blocking position blocking the communication groove (51) and the opening position opening the communication groove (51); and a spring member (54) energizing the shutter member (53) to the blocking position. |
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| 175 | ROTARY DEVICE FOR BIOLOGICAL PRINTING, AND METHOD OF USE THEREOF | EP15886868.7 | 2015-03-31 | EP3278765A1 | 2018-02-07 | KANG, Yujian James; ZHOU, Huixing |
The present invention relates to a rotary rod for 3D bio-printing, in which the rotary rod is arranged horizontally and is driven to rotate, the rotary rod has a hollow structure and provided with at least one hole in a surface thereof, such that during a 3D bio-printing process, a nutrition solution passes through the hollow structure and a portion of the nutrition solution exudes via at least one hole. The present invention further provides a 3D bio-printing platform for supplying nutrition, comprising the rotary rod and a nutrition supply system, and a method of printing a tubular tissue using the bio-printing platform. The present invention, which reduces the possibility of resulting in tissue collapse from the effect of gravity, provides a new method of 3D bio-printing a tubular tissue and supplying nutrition in a printing process, with a wide application prospect. |
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| 176 | ENDODERMAL CELL PRODUCTION METHOD, LIVER CELL PRODUCTION METHOD, PANCREATIC CELL PRODUCTION METHOD, ENDODERMAL CELL INDUCTION PROMOTER, LIVER CELL INDUCTION PROMOTING KIT, PANCREATIC CELL INDUCTION PROMOTING KIT, AND MICRO FLUID DEVICE | EP15873109.1 | 2015-12-22 | EP3239293A1 | 2017-11-01 | KAMEI Kenichiro; HONDA Momoko |
The present invention provides an endodermal cell production method that can induce differentiation of pluripotent cells into endodermal cells even when the pluripotent cells are dispersed and can achieve improved endodermal cell production efficiency. The endodermal cell production method according to the present invention is a method for producing endodermal cells by inducing differentiation of pluripotent cells into the endodermal cells, including the step of inducing differentiation of the pluripotent cells into the endodermal cells in the presence of an endodermal cell inducing factor. In the induction step, the cell density of the pluripotent cells at the start of the induction preferably is from 0.5 × 104 to 2 × 104 cells/cm2. |
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| 177 | CELL SHEET MANUFACTURING DEVICE AND MANUFACTURING METHOD THEREFOR | EP15859223.8 | 2015-11-09 | EP3219785A1 | 2017-09-20 | KIM, Daehyeong; CHOI, Seunghong; HYEON, Taeghwan; KIM, Seokjoo; CHO, Hyerim; CHO, Kyoungwon |
The present invention relates to a cell sheet manufacturing device and a manufacturing method therefor. More specifically, the present invention relates to a cell sheet manufacturing device comprising a support layer made of silicon rubber, a patterned electrode formed adjacent to the support layer and a graphene layer formed adjacent to the electrode, and a manufacturing method therefor. |
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| 178 | CANCER CELL DETECTION METHOD USING CELLS OF BIOLOGICAL ORIGIN | EP15844697.1 | 2015-09-24 | EP3199638A1 | 2017-08-02 | YAMADA, Katsuya; SASAKI, Ayako; ONO, Kouki; TONE, Kiyoshi |
The purpose of the present invention is to provide a novel cancer cell detection method that uses living body-derived cells and that can be used even in cytodiagnosis. In particular, the purpose of the present invention is to provide a novel cancer cell detection method that makes it possible to perform imaging of cells in a living state and a dual detection method for cancer cells in which the aforementioned method is combined with a pre-existing dyeing method for cytodiagnosis. Provided is a cancer cell detection method that uses living body-derived cells and that includes: incubating living cells included in a sample taken from a person together with a fluorescently-labeled L-glucose derivative and detecting the fluorescently-labeled L-glucose derivative that is taken up into the cells; and detecting fluorescence emitted by the L-glucose derivative that is present within the cells while the cells are attached to a thin glass or plastic plate. Also provided is a dual detection method for cancer cells in which the cancer cell detection method that uses living body-derived cells is combined with a dyeing method using cells that are fixed using an alcohol or the like. |
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| 179 | METHOD AND DEVICE FOR CONTROLLING PATTERN AND STRUCTURE FORMATION BY AN ELECTRIC FIELD | EP12753614.2 | 2012-07-31 | EP2740143A2 | 2014-06-11 | BRCKA, Jozef; FAGUET, Jacques; LEE, Eric, M.; YUE, Hongyu |
| A processing method and apparatus uses at least one electric field applicator (34) biased to produce a spatial-temporal electric field to affect a processing medium (26), suspended nano-objects (28) or the substrate (30) in processing, interacting with the dipole properties of the medium (26) or particles to construct structure on the substrate (30). The apparatus may include a magnetic field, an acoustic field, an optical force, or other generation device. The processing may affect selective localized layers on the substrate (30) or may control orientation of particles in the layers, control movement of dielectrophoretic particles or media, or cause suspended particles of different properties to follow different paths in the processing medium (26). Depositing or modifying a layer on the substrate (30) may be carried out. Further, the processing medium (26) and electrical bias may be selected to prepare at least one layer on the substrate (30) for bonding the substrate (30) to a second substrate, or to deposit carbon nanotubes (CNTs) with a controlled orientation on the substrate. | ||||||
| 180 | SYSTEM AND METHOD FOR TISSUE CONSTRUCTION USING AN ELECTRIC FIELD APPLICATOR | EP12753276.0 | 2012-07-31 | EP2739719A2 | 2014-06-11 | BRCKA, Jozef |
| A method and apparatus are provided for constructing tissue from cells or other objects by application of temporally and spatially controlled electric fields. Electric field applicators expose a substrate (32) to the electric field controlled to affect the processing medium (28) to achieve a processing effect on the construction of tissue on the substrate (32). Electrical bias is selected to interact with dipole properties of the medium (28) to control the movement of suspended dielectrophoretic cells or other particles in the medium (28) or at the substrate (32). The motion of suspended particles may be affected to cause suspended particles of different properties to follow different paths in the processing medium (28), which may be used to cause the suspended particles to be sorted. The processing medium (28) and electrical bias may be selected to affect the structure, or orientation, of one or more layers on the substrate (32). | ||||||
