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序号 专利名 申请号 申请日 公开(公告)号 公开(公告)日 发明人
81 IMPROVEMENT OF PLANT FLOWER TYPE TARGETING MADS BOX GENE PCT/JP2001/009511 2001-10-30 WO02036776A1 2002-05-10
A genomic DNA containing the promoter domain of pMADS3 is isolated, ligated to a reporter gene and then transferred into petunia plant via an agrobacterium. As a result, the stamen of this plant surprisingly turns into a petal-like structure, thereby presenting double flowers.
82 A METHOD FOR INHIBITING THE EXPRESSION OF TARGET GENES IN PLANTS PCT/EP2000/001524 2000-02-09 WO00047754A1 2000-08-17
This invention concerns a method for inhibiting the expression of target genes in plants wherein a chimeric DNA construct comprising at least one repressor sequence in transcriptional fusion with at least one plant-specific sequence that codes for a protein or fragment thereof that bind to DNA or that activates transcription either by binding to DNA itself or by interacting with a DNA-binding protein.
83 TARGETED SCREENING FOR NOVEL DISEASE RESISTANCE IN PLANTS PCT/NL2015/050388 2015-05-29 WO2015183096A1 2015-12-03 BAKKER, Jaap; HELDER, Johannes

The present invention relates to a screening method for the identification of non-genetically modified plants with a specific resistance response to a pathogen, the method involving the transgenic expression of a putative R gene construct, the identification of polymorphic markers in the putative R gene by comparing its sequence with known R gene sequences, and screening of non-genetically modified plant populations for the presence of these polymorphic markers.

84 OPTIMISING OBJECTIVES OF A PLANT AND A CARBON DIOXIDE CAPTURE FACILITY PCT/AU2011/000689 2011-06-02 WO2011150462A1 2011-12-08 HOADLEY, Andrew Forbes Alexander; HARKIN, Trent Liam

The present invention relates to a method and system for optimising objectives of a plant and a carbon dioxide capture facility. The method comprises a multi-objective algorithm and includes the use of process simulation packages and linear programming programs to optimise objectives. The objectives may include an optimal configuration for capturing carbon dioxide from emissions of a power plant via the carbon dioxide capture facility.

85 IDENTIFICATION AND USE OF TARGET GENES FOR CONTROL OF PLANT PARASITIC NEMATODES PCT/US2007061932 2007-02-09 WO2007095469A2 2007-08-23 BOUKHAROV ANDREY A; DU ZIJIN; GUO LIANG; HRESKO MICHELLE C; KOVALIC DAVID K; LI ZHALOLONG; LU MAOLONG; MCCARTER JAMES P; MILLER NANCY M; VAUDIN MARK; WILLIAMS DERYCK J; WU WEI
The invention relates to identifying and evaluating target coding sequences for control of plant parasitic nematodes by inhibiting one or more biological functions, and their use. The invention provides methods and compositions for identification of such sequences and for the control of a plant-parasitic nematode population. By feeding one or more recombinant double stranded RNA molecules provided by the invention to the nematode, a reduction in disease may be obtained through suppression of nematode gene expression. The invention is also directed to methods for making transgenic plants that express the double stranded RNA molecules, and the plant cells and plants obtained thereby.
86 PROCESSES AND VECTORS FOR PRODUCING TRANSGENIC PLANTS PCT/EP0113662 2001-11-23 WO0246438A3 2003-02-27 GLEBA YURI; KLIMYUK VICTOR; BENNING GREGOR; ELIBY SERIK
This invention describes a process for gene expression in plants utilizing translational vectors. Said translational vectors cause a gene of interest to be stably integrated into a transcriptionally active host genomic DNA such that the transcription the gene of interest is controlled by a promoter of the host organism. Said translational vectors are preferably based on internal ribosome entry site (IRES) elements that are of plant origin, plant viral origin, synthetic origin or are isolated from other organisms.
87 PROCESSES AND VECTORS FOR PRODUCING TRANSGENIC PLANTS PCT/EP2002/006464 2002-06-12 WO2002101060A1 2002-12-19 KLIMYUK, Victor; BENNING, Gregor; ELIBY, Serik; GLEBA, Yuri

A process for producing transgenic plants or plant cells capable of expressing a coding sequence of interest under transcriptional and translational control of host nuclear transcriptional and translational elements is described by introducing into the nuclear genome of host plants or plant cells a vector comprising said coding sequence of interest which is devoid of (a) an upstream element of initiation of transcription functional in the host plants or plant cells and operably linked to said coding sequence of interest and required for its transcription; (b) an upstream element of initiation of translation functional in the host plants or plant cells and operably linked to said coding sequence of interest; and subsequently selecting plant cells or plants expressing said coding sequence of interest.

88 TISSUE-SPECIFIC EXPRESSION OF TARGET GENES IN PLANTS PCT/CA2001/000066 2001-01-29 WO01055433A2 2001-08-02
Methods of producing plants having tissue-specific expression of one or more target genes are taught, as are methods of producing plants having tissue-specific and environmental or developmentally-regulated expression of one or more target genes. A genetic construct is taught which contains a nitrogen assimilation and/or metabolism gene and a promoter for the gene which is inducible under conditions where it would be beneficial to take up, store or use nitrogen. The promoter can be, for example, induced by the presence of nitrate or other form of nitrogen and will be induced by application of a nitrogenous fertilizer. The stress inducible promoter btg-26 is also taught. Promoter btg-26 is isolated from the Brassica turgor gene - 26 and exhibits osmotic-stress induced and tissue-specific expression of an operatively linked gene. The present invention also provides for methods to produce plants with increased biomass and seed yield.
89 목표유전자의 식물세포 내로의 도입을 증가시키는 방법 KR1020050071372 2005-08-04 KR1020070016577A 2007-02-08 김율호; 이장용; 손수인; 이병규; 이명순; 홍미영; 김주곤; 조준형
본 발명은 유전자를 식물체에 형질전환시키는 방법 및 이를 이용하여 제조된 식물체에 관한 것으로서, 보다 상세하게는 아그로박테리움을 식물체별 형질전환이 가능한 목적 조직에 접종한 후 아그로박테리움 내의 재조합 플라스미드에 포함된 목표유전자를 식물세포 내에 도입하는 단계를 포함하는 공동배양 과정에서의 배지조성과 조건 등을 새로이 개량함으로써 형질전환 효율을 높이는 방법에 관한 것이다. 또한, 본 발명의 개량된 형질전환 방법을 이용하여 형질전환이 어려운 벼 유전자형의 품종을 대상으로 형질전환 식물체를 생산할 수 있다. 벼, 아그로박테리움, 공동배양과정, 배지조성 및 조건, 형질전환 식물체
90 목적 단백질을 발현하는 식물세포, 이의 제조방법 및 이를 이용한 목적 단백질의 생산방법 KR1020140024160 2014-02-28 KR1020140108174A 2014-09-05 진영우; 이은경; 장미옥; 박보라; 이수란; 양보림; 김일숙; 오일석
The present invention relates to plant cells expressing target proteins, a producing method thereof and a method of producing target proteins using the same. Recombinant vectors containing gene coding target proteins are introduced in the plant cells, and the plant cells include cambial meristematic cells (CMC) or callus. Here, CMC as plant-derived cell line, separated from cambium tissue of a plant, containing naturally undifferentiated cells separated from a plant is characterized to have degradation-systematic continuity which does not go through a dedifferentiation process to callus. A target protein expressing system according to the present invention is useful because bio medicine and medical supplies such as plant-derived protein products or the like can be commercialized by relieving the existing problems of cultivating plant cells, and through mass-producing target proteins including biopharmaceutical proteins due to innovative transgenic rate.
91 標的DNAに変異が導入された植物細胞、及びその製造方法 PCT/JP2015/054610 2015-02-19 WO2015125862A1 2015-08-27 横井 彩子; 土岐 精一

 標的DNAと相同なDNAを含むDNA構築物であって、該相同DNAにマーカー遺伝子を含むpiggyBacトランスポゾンが挿入されており、かつ所望の変異が導入されているDNA構築物を、植物細胞に導入する工程と、 前記変異及び前記piggyBacトランスポゾンが相同組換えによって標的DNAに導入された植物細胞を、前記マーカー遺伝子の発現を指標として選択する工程と、 前記工程にて選択された細胞に、piggyBacトランスポゼースを恒常的に発現させ、前記piggyBacトランスポゾンを前記標的DNAより除去する工程とを含む、標的DNAに変異が導入された植物細胞の製造方法。

92 METHODS OF QUANTIFYING TARGET ORGANISMS AND CREATING RENIFORM RESISTANT COTTON PLANTS PCT/US2010/052157 2010-10-11 WO2011044550A1 2011-04-14 BHATTI, Muhammad; CANTRELL, Roy, G.; HENDRIX, Bill, L.; KOHLFELD, Patsy, L.; WU, Kunsheng; XIAO, Jinhau

The present invention is in the field of plant breeding and disease resistance. More specifically, the invention includes methods for assaying a location to determine the amount of pest infestation, or assaying a plant for its ability to resist infection, and using this information to make agronomic treatment and/or breeding decisions. The invention also provides methods for breeding cotton plants containing one or more quantitative trait loci that are associated with resistance to reniform nematode infection. The invention further includes germplasm and the use of germplasm containing quantitative trait loci (QTL) conferring reniform resistance as a source of reniform resistant alleles for introgression into elite germplasm in a breeding program, thus producing novel elite germplasm comprising one or more reniform resistance loci.

93 SHORT FRAGMENT HOMOLOGOUS RECOMBINATION TO EFFECT TARGETED GENETIC ALTERATIONS IN PLANTS PCT/US2003/001734 2003-01-21 WO2003062425A1 2003-07-31 PROKOPISHYN, Nicole, Lesley

The invention concerns the application of short fragment homologous recombination (SFHR) to plant cells. In particular, the invention concerns the type of SFHR in which the short fragment is a single stranded nucleic acid preparation of one strand that is substantially free of the complementary strand.

94 TARGET GENES FOR CONTROL OF PLANT PARASITIC NEMATODES AND USE OF SAME PCT/IB2011/002130 2011-09-13 WO2012035407A2 2012-03-22 FOSU-NYARKO, John; JONES, Michael, George, Kepler

The invention relates to identifying and evaluating target coding and non-coding sequences for control of plant parasitic nematodes by inhibiting one or more biological functions, and their use. The invention provides methods and compositions for identification of such sequences and for the control of a plant-parasitic nematode population. By feeding one or more recombinant double stranded RNA molecules provided by the invention to the nematode, a reduction in disease may be obtained through suppression of nematode gene expression. The invention is also directed to methods for making transgenic plants that express the double stranded RNA molecules, and the plant cells and plants obtained thereby.

95 СПОСОБ СУПЕРПРОДУКЦИИ В РАСТЕНИИ ЦЕЛЕВОГО БЕЛКА PCT/RU2006/000627 2006-11-24 WO2008063093A1 2008-05-29 ДОРОХОВ Юрий Леонидович; КОМАРОВА Татьяна Валерьевна; АТАБЕКОВ Иосиф Григорьевич

Изобретение относится к биотехнологии, генной инженерии и медицине и обеспечивает способ продукции (суперпродукции) в клетке растения чужеродных или эндогенных целевых белков (ЦБ) Суперпродукция ЦБ достигается в результате экспрессии в клетке растения векторов, направляющих синтез РНК особого типа, обозначаемых как декодирующие PHK» (нкРНК), то есть РНК, не способные кодировать белок. Нами показано, что нкРНК резко стимулируют продукцию ЦБ, причём стимулирующей активностью обладают нкРНК как природного, так и искусственного происхождения. Способ универсален и обеспечивает стимуляцию накопления в клетках растений любых целевых белков, включая вакцинные и терапевтические ЦБ.

96 PROCESS FOR PURIFYING TARGET COMPOUNDS FROM PLANT SOURCES USING CERAMIC FILTRATION PCT/US2005044325 2005-12-10 WO2006063085A2 2006-06-15 GARGER STEPHEN J; BRATCHER BARRY; VOJDANI FAKHRIEH
The present invention describes a method for isolating a target compound from a plant, the method comprising obtaining a plant extract, passing such plant extract through a ceramic filter to obtain a permeate, and purifying the target compound from such permeate. This method, among other things, allows ultrafiltration of crude plant extracts, such as green juice homogenates.
97 엽록체에서 목적 단백질을 대량 생산하는 형질전환 식물체의 제조 방법 및 그에 따른 식물체 KR1020120102377 2012-09-14 KR1020140035735A 2014-03-24 정영희; 마상훈
The present invention provides: a recombinant vector for targeting a target protein comprising any one base sequence among the base sequences coding four kinds of transit peptides derived from PsbP protein, a kind of oxygen evolving protein (OEC) of tobacco, to a chloroplast of a plant; plant cells transformed with the recombinant vector; a method for producing the target protein in large quantities in the chloroplast of the plant by transferring and accumulating the target protein to the chloroplast of the plant, the method comprises a step for transforming the plant cells with the recombinant vector; a method for manufacturing a transgenic plant producing the target protein in large quantities in the chloroplast, the method comprises a step for transforming the plant cells with the recombinant vector; the transgenic plant producing the target protein produced by the same method in large quantities in the chloroplast; a method for producing the target protein in large quantities in the chloroplast by transferring and accumulating the target protein from a plant tissue to the chloroplast, the method comprises a step for transiently expressing the target protein by introducing the recombinant vector into the plant tissue; and a composition for targeting the chloroplast of the plant producing the target protein comprising any one base sequence among the base sequences coding four kinds of the transit peptides.
98 Method for producing transgenic plant producing target protein massively in chloroplast and the plant thereof KR20150000514 2015-01-05 KR20150011019A 2015-01-29 JOUNG YOUNG HEE; MA SANG HOON
본 발명은 담배의 산소 발생 단백질(oxygen evolving protein, OEC)의 일종인 PsbP 단백질 유래의 4종의 트랜지트 펩타이드(transit peptide) 코딩 염기서열 중 어느 하나를 포함하는 목적 단백질을 식물의 엽록체로 타겟팅하기 위한 재조합 벡터, 상기 재조합 벡터로 형질전환된 식물세포, 상기 재조합 벡터로 식물세포를 형질전환시키는 단계를 포함하는 목적 단백질을 식물의 엽록체로 이동 및 축적시켜 목적 단백질을 식물의 엽록체에서 대량 생산하는 방법, 상기 재조합 벡터로 식물세포를 형질전환하는 단계를 포함하는 목적 단백질을 엽록체에서 대량 생산하는 형질전환 식물체의 제조 방법, 상기 방법에 의해 제조된 목적 단백질을 엽록체에서 대량 생산하는 형질전환 식물체, 재조합 벡터를 식물 조직에 도입하여 목적 단백질을 일시적으로 발현(transient expression)하는 단계를 포함하는 식물 조직으로부터 목적 단백질을 엽록체로 이동 및 축적시켜 목적 단백질을 엽록체에서 대량 생산하는 방법 및 상기 4종의 트랜지트 펩타이드 코딩 염기서열 중 어느 하나를 포함하는 목적 단백질의 식물의 엽록체 타겟팅용 조성물을 제공한다.
99 植物における改善された標的に向けたDNA挿入 JP2011108596 2011-05-13 JP5913833B2 2016-04-27 ダルウィン,キャスリーン; ファンデルストラエテン,シャンタル; ルイター,レーネ
100 Transgenic plant highly accumulating target protein JP2010168163 2010-07-27 JP2012024052A 2012-02-09 WASAI MASASHI; KASAHARA SAORI
PROBLEM TO BE SOLVED: To provide a transgenic plant in which the yield nature of a desired function component is improved.SOLUTION: There are disclosed: a transgenic plant which has introduced a polynucleotide which encodes a target protein, and a polynucleotide which encodes a nitrogen transporter; a method of cultivating the transgenic plant; a production method of a seed of the transgenic plant which includes growing the transgenic plant and collecting the seed; an expression vector which includes a polynucleotide which encodes a target protein and a polynucleotide which encodes a nitrogen transporter; and a set of an expression vector which includes a polynucleotide which encodes the target protein and an expression vector which includes a polynucleotide which encodes a nitrogen transporter.
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