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序号 专利名 申请号 申请日 公开(公告)号 公开(公告)日 发明人
161 Nouvelles cibles pour herbicides et plantes transgéniques résistantes à ces herbicides EP07115389.4 2001-12-05 EP1862553A3 2008-04-09 Matringe, Michel; Rippert, Pascal

La présente invention a pour objet de nouvelles enzymes possédant une activité arogénate déhydrogénase, en particulier des enzymes arogénate déhydrogénase de plantes, ainsi que les gènes codant ces enzymes. Les enzymes arogénate déhydrogénase selon l'invention catalysent la dernière étape de la voie métabolique de biosynthèse de la tyrosine, et constituent, à ce titre, des cibles potentielles d'herbicides. La présente invention concerne donc également un procédé d'identification de composés herbicides ayant pour cible ces enzymes, lesdits composés herbicides empêchant la biosynthèse de tyrosine en se fixant sur lesdites enzymes. L'invention a également pour objet des plantes transgénique tolérantes à des composés herbicides ayant pour cible une enzyme impliquée dans la voie de biosynthèse de la tyrosine, en particulier une enzyme impliquée dans la transformation du préphénate en L-tyrosine, en particulier une enzyme arogénate déhydrogénase. Ces plantes deviennent tolérantes par expression dans leurs tissus d'une enzyme préphénate dehydrogénase, cette enzyme étant insensible aux dits composés herbicides et permettant à la plante de synthétiser la tyrosine malgré un traitement par lesdits composés herbicides.

162 Nouvelles cibles pour herbicides et plantes transgéniques résistantes à ces herbicides EP07115389.4 2001-12-05 EP1862553A2 2007-12-05 Matringe, Michel; Rippert, Pascal

La présente invention a pour objet de nouvelles enzymes possédant une activité arogénate déhydrogénase, en particulier des enzymes arogénate déhydrogénase de plantes, ainsi que les gènes codant ces enzymes. Les enzymes arogénate déhydrogénase selon l'invention catalysent la dernière étape de la voie métabolique de biosynthèse de la tyrosine, et constituent, à ce titre, des cibles potentielles d'herbicides. La présente invention concerne donc également un procédé d'identification de composés herbicides ayant pour cible ces enzymes, lesdits composés herbicides empêchant la biosynthèse de tyrosine en se fixant sur lesdites enzymes. L'invention a également pour objet des plantes transgénique tolérantes à des composés herbicides ayant pour cible une enzyme impliquée dans la voie de biosynthèse de la tyrosine, en particulier une enzyme impliquée dans la transformation du préphénate en L-tyrosine, en particulier une enzyme arogénate déhydrogénase. Ces plantes deviennent tolérantes par expression dans leurs tissus d'une enzyme préphénate dehydrogénase, cette enzyme étant insensible aux dits composés herbicides et permettant à la plante de synthétiser la tyrosine malgré un traitement par lesdits composés herbicides.

163 Insect-resistant transgenic plants and methods for improving delta-endotoxin activity against target insects EP06118584.9 1998-12-17 EP1749834B1 2012-04-25 English, Leigh; Brussock, Susan M.; Malvar, Thomas M.; Bryson, James W.; Kulesza, Caroline A.; Walters, Frederick S.; Slatin, Stephen L.; von Tersch, Michael A.; Romano, Charles
164 PLANT CELL COMPRISING MUTATION INTRODUCED IN TARGET DNA, AND METHOD FOR PRODUCING THE PLANT CELL US15119425 2015-02-19 US20170058285A1 2017-03-02 Ayako YOKOI; Seiichi TOKI
A method for producing a plant cell comprising a mutation introduced in a target DNA comprises: a step of introducing into plant cells a DNA construct comprising a DNA homologous to a target DNA, wherein a desired mutation is introduced and a piggyBac transposon containing a marker gene is inserted in the homologous DNA; a step of selecting a plant cell, in which the mutation and the piggyBac transposon are introduced in the target DNA via homologous recombination, based on an expression of the marker gene; and a step of removing the piggyBac transposon from the target DNA by constitutively expressing a piggyBac transposase in the cell selected in the above step.
165 PLANT CELL COMPRISING MUTATION INTRODUCED IN TARGET DNA, AND METHOD FOR PRODUCING THE PLANT CELL US15121458 2015-02-24 US20160362699A1 2016-12-15 Masaki IWAKAMI; Seiichi TOKI
It has been found that a marker gene, which is inserted in the genomic DNA via homologous recombination, and to both ends of which nuclease recognition sites are added, can be removed from a plant cell by using a corresponding nuclease, and further that the nuclease recognition sites can also be removed without leaving any trace by matching sequences of at least 30 nucleotides adjacent to the recognition sites. Moreover, in a method for introducing a mutation into a target DNA on the genome of a plant cell via homologous recombination, it is made possible to: stably select a plant cell, in which the mutation is introduced, based on an expression of a marker gene; further, to remove an unnecessary sequence such as the marker gene from the selected cell; and to introduce only a required mutation into the target DNA.
166 標的化したDNA配列の核酸塩基を特異的に変換する、単子葉植物のゲノム配列の変換方法、及びそれに用いる分子複合体 PCT/JP2016/085075 2016-11-25 WO2017090761A1 2017-06-01 西田 敬二; 島谷 善平; 近藤 昭彦

本発明は、単子葉植物細胞の有する二本鎖DNAの標的化された部位を改変する方法であって、選択された二本鎖DNA中の標的ヌクレオチド配列と特異的に結合する核酸配列認識モジュールと、核酸塩基変換酵素とが結合した複合体を、該二本鎖DNAと接触させ、該標的化された部位において該二本鎖DNAの少なくとも一方の鎖を切断することなく、該標的化された部位の1以上のヌクレオチドを他の1以上のヌクレオチドに変換する又は欠失させる、あるいは該標的化された部位に1以上のヌクレオチドを挿入する工程を含み、該二本鎖DNAと該複合体との接触が、該単子葉植物細胞への該複合体をコードする核酸の導入により行われる、方法を提供する。さらに、当該方法に使用するための、単子葉植物細胞の有する二本鎖DNA中の標的ヌクレオチド配列と特異的に結合する核酸配列認識モジュールと、核酸塩基変換酵素とが結合した複合体も提供する。

167 NUCLEIC ACID AGENTS FOR OVEREXPRESSING OR DOWNREGULATING RNA INTERFERENCE TARGETS AND USES OF SAME IN IMPROVING NITROGEN USE EFFICIENCY, ABIOTIC STRESS TOLERANCE, BIOMASS, VIGOR OR YIELD OF A PLANT PCT/IB2012/054149 2012-08-14 WO2013024440A1 2013-02-21 MAOR, Rudy; NESHER, Iris; NOIVIRT, Orly

A method of improving nitrogen use efficiency, abiotic stress tolerance, biomass, vigor or yield of a plant is provided by expressing within the plant an exogenous polynucleotide encoding a polypeptide having an amino acid sequence at least 80 % homologous to SEQ ID NOs: 687-981, 992-1248, 1281-1310, 1389-1391, and 2806-3081. Also provided is a method of improving nitrogen use efficiency, abiotic stress tolerance, biomass, vigor or yield of a plan by expressing within the plant an exogenous polynucleotide which downregulates an activity or expression of a polypeptide having an amino acid sequence at least 80 % homologous to SEQ ID NOs: 311-514, 2007-2436, 1311-1320, 982-991, 1249-1280, 1321-1388. Transgenic plants and constructs are provided as well.

168 COMPOSITIONS AND METHODS FOR ACHIEVING A BIOLOGICAL EFFECT IN TARGET VEGETATION US15176610 2016-06-08 US20160353747A1 2016-12-08 Jeffrey T. Bargiel; Keith D. Wing
Compositions and methods are used to degrade; decay; kill; stunt; decompose; slow, stop, prevent growth or regrowth of; compost; wound; or break down vegetation or lignocellulosic material.
169 Methods of quantifying target organisms and creating reniform resistant cotton plants US12901756 2010-10-11 US08686219B2 2014-04-01 Muhammad Bhatti; Roy G. Cantrell; Bill L. Hendrix; Patsy L. Kohlfeld; Kunsheng Wu; Jinhua Xiao
The present invention is in the field of plant breeding and disease resistance. More specifically, the invention includes methods for assaying a location to determine the amount of pest infestation, or assaying a plant for its ability to resist infection, and using this information to make agronomic treatment and/or breeding decisions. The invention also provides methods for breeding cotton plants containing one or more quantitative trait loci that are associated with resistance to reniform nematode infection. The invention further includes germplasm and the use of germplasm containing quantitative trait loci (QTL) conferring reniform resistance as a source of reniform resistant alleles for introgression into elite germplasm in a breeding program, thus producing novel elite germplasm comprising one or more reniform resistance loci.
170 TARGET GENES FOR CONTROL OF PLANT PARASITIC NEMATODES AND USE OF SAME US13823082 2011-09-13 US20130269057A1 2013-10-10 John Fosu-Nyarko; Michael George Kepler Jones
The invention relates to identifying and evaluating target coding and non-coding sequences for control of plant parasitic nematodes by inhibiting one or more biological functions, and their use. The invention provides methods and compositions for identification of such sequences and for the control of a plant-parasitic nematode population. By feeding one or more recombinant double stranded RNA molecules provided by the invention to the nematode, a reduction in disease may be obtained through suppression of nematode gene expression. The invention is also directed to methods for making transgenic plants that express the double stranded RNA molecules, and the plant cells and plants obtained thereby.
171 TARGET GENES FOR CONTROL OF PLANT PARASITIC NEMATODES AND USE OF SAME US14411888 2013-06-28 US20150176024A1 2015-06-25 John Fosu-Nyarko; Michael George Kepler Jones
The invention relates to identifying and evaluating target coding and non-coding sequences for control of plant parasitic nematodes by inhibiting one or more biological functions, and their use. The invention provides methods and compositions for identification of such sequences and for the control of a plant-parasitic nematode population. By feeding one or more recombinant double-stranded RNA molecules provided by the invention to the nematode, a reduction in disease may be obtained through suppression of nematode gene expression. The invention is also directed to methods for making transgenic plants that express the double-stranded RNA molecules, and the plant cells and plants obtained thereby.
172 METHODS OF QUANTIFYING TARGET ORGANISMS AND CREATING RENIFORM RESISTANT COTTON PLANTS US12901756 2010-10-11 US20110088118A1 2011-04-14 Muhammad Bhatti; Roy G. Cantrell; Bill L. Hendrix; Patsy L. Kohlfeld; Kunsheng Wu; Jinhua Xiao
The present invention is in the field of plant breeding and disease resistance. More specifically, the invention includes methods for assaying a location to determine the amount of pest infestation, or assaying a plant for its ability to resist infection, and using this information to make agronomic treatment and/or breeding decisions. The invention also provides methods for breeding cotton plants containing one or more quantitative trait loci that are associated with resistance to reniform nematode infection. The invention further includes germplasm and the use of germplasm containing quantitative trait loci (QTL) conferring reniform resistance as a source of reniform resistant alleles for introgression into elite germplasm in a breeding program, thus producing novel elite germplasm comprising one or more reniform resistance loci.
173 METHOD FOR PRODUCING TARGET PROTEINS USING AMINO ACIDS AND PYRUVIC ACIDS IN CULTURE OF PLANT CELLS US12160049 2007-09-19 US20090181428A1 2009-07-16 Sang-Lin Kim; Hyun-Kwang Tan; Sang-Min Lim; Wuk-Sang Ryu; Hahn-Sun Jung; Song-Jae Lee; Cheon-Ik Park; Seung-Hoon Kang; Dong-Il Kim
Provided is a method for producing a target protein via cultivation of transgenic plant cells comprising a promoter capable of expressing the protein under sugar-free conditions or in response to the depletion of sugar and a gene encoding the target protein, without exchange of a cell growth medium with a sugar-depleted medium. The method comprises 1) culturing transgenic plant cells in a sugar-rich medium to grow plant cells; and 2) culturing the transgenic plant cells with addition of an amino acid mixture to the culture of Step 1 without exchange of a cell growth medium with a sugar-depleted medium, thereby expressing a target protein. The method of the present invention enables commercial-scale production of recombinant proteins via establishment of optimized culture conditions by addition of an amino acid mixture to induce protein expression without exchange of a cell growth medium with a sugar-free medium, and addition of pyruvic acid during the induction period of protein expression to thereby enhance the production yield of target proteins.
174 Use of spray adjuvant to enhance the movement of pesticides through plant canopies to the target US10972624 2004-10-25 US20060088563A1 2006-04-27 Donald Penner
Pesticide compositions and methods of using the compositions for killing, inhibiting or repelling pests are disclosed. The pesticide compositions are admixtures of at least one pesticide and a repellent adjuvant. The retention of the pesticide compositions on the foliage of plants is reduced, so that the composition facilitates the penetration of the foliage onto the underlying target site. Preferably the composition is applied as a spray to provide spherically shaped particles which bounce off the foliage. The pesticide composition can be used to penetrate natural foliage such as tree canopies and underbrush, or to penetrate through cultivated foliage such as turfgrass and crops.
175 Method and apparatus for target plant foliage sensing and mapping and related materials application control US66137 1987-06-23 US4823268A 1989-04-18 Durham K. Giles; Michael J. Delwiche; Roy B. Dodd
An electronic orchard tree measuring system based on ultrasonic range transducers may be used to determine the amount and vertical distribution of sensed load centroids in vertical sectors of orchard trees. The ultrasonic transducers may be operated independently of any processor or memory which receives their distance data outputs. Displacement sensing of the apparatus relative a row of target trees may instead be used to select ultrasonic distance data at predetermined incremental distances of travel relative the trees being sensed. Thus, the most recent value of sensed data is always available as input to the processor or memory, without requiring any timing circuitry between such sensing and control circuits. The detection data may be stored in memory for subsequent processing to create a map of foliar volumes of the sensed target trees. Alternatively, the spatial characteristics of the sensed amount and vertical distribution of load centroids for a given vertical segment of target trees may be further compared spatially with determined application patterns of controllable spray nozzles to configure subsequent activation of such nozzles for optimal material application to the given vertical segment of the sensed target trees.
176 VIRUS LIKE PARTICLES COMPRISING TARGET PROTEINS FUSED TO PLANT VIRAL COAT PROTEINS US13496568 2010-09-16 US20120219579A1 2012-08-30 Vidadi Yusibov; Christine E. Farrance; Konstantin A. Musiychuk; Vadim Mett; Valentina Mett
Virus like particles comprising a fusion protein and substantially free of nucleic acid, wherein the fusion protein comprises a plant viral coat protein and a target protein, are provided. Immunogenic compositions comprising the virus like particles can be administered to subjects to induce protective immune responses in the subjects. Methods of producing the virus like particles are also provided.
177 Process for purifying target compounds from plant sources using ceramic filtration US11301469 2005-12-12 US20060288449A1 2006-12-21 Stephen Garger; Barry Bratcher; Fakhrieh Vojdani
The present invention describes a method for isolating a target compound from a plant, the method comprising obtaining a plant extract, passing such plant extract through a ceramic filter to obtain a permeate, and purifying the target compound from such permeate. This method, among other things, allows ultrafiltration of crude plant extracts, such as green juice homogenates.
178 Accumulation of fructans in plants by targeted expression of bacterial levansucrase US640732 1996-05-06 US5908975A 1999-06-01 Perry Gerard Caimi; Howard Paul Hershey; Phillip S. Kerr
This invention concerns methods for synthesis and accumulation of fructose polymers in seed, tubers or leaves of transgenic plants by selective expression of a bacterial fructosyltransferase gene. Selective expression includes coordination of timing, tissue specific expression and especially subcellular location. Successful transformants utilize sucrose to synthesize and accumulate fructan in the vacuole of the cell, in established crops, without loss of co-products or concern for yield loss due to degradation during maturation, harvest or storage of the plant. Enhanced fructan production will benefit the fructose sweetener industry and add value to grain used for feed.
179 RECOMBINANT PLANT CELL, PREPARATION METHOD THEREFOR, AND METHOD FOR PRODUCING TARGET PROTEIN USING SAME US14771483 2014-02-28 US20160010099A1 2016-01-14 Young Woo JIN; Eun Kyong LEE; Mi Ok JANG; Bora PARK; Soo Ran LEE; Bo Rim YANG; Il Suk KIM; Il Seok OH
Plant cells express a target protein, a production method thereof, and a method of producing a target protein using the same are disclosed. The plant cells have introduced therein a recombinant vector containing gene(s) encoding a target protein, and the plant cells include plant cambial meristematic cells (CMC) or callus. The cambial meristematic cells (CMCs) are a cell line containing innately undifferentiated cells isolated from a plant, wherein the cell line is a cell line isolated from the cambial tissue of the plant and has meristematic continuity without going through dedifferentiation into callus. A system of expressing a target protein using the recombinant plant cells according to the present invention can overcome the problems of conventional plant cell culture. In addition, it shows significantly high transformation efficiency, and thus can produce large amounts of target proteins, including biopharmaceutical proteins.
180 USE OF DOUBLE STRANDED RNA TO INCREASE THE EFFICIENCY OF TARGETED GENE ALTERATION IN PLANT PROTOPLASTS US13141196 2009-12-22 US20110312094A1 2011-12-22 Paul Bundock
Method for targeted gene alteration in protoplasts of plant cells comprising the steps of transiently transfecting the protoplasts with a dsRNA that preferably targets plant MMR mRNA; and a mutagenic nucleobase. The transfection may be simultaneously or subsequently and the gene can be any gene functional in the mismatch repair system.
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