| 序号 | 专利名 | 申请号 | 申请日 | 公开（公告）号 | 公开（公告）日 | 发明人 |
|---|---|---|---|---|---|---|
| 61 | 构建水稻白叶枯病菌Ⅲ型分泌系统转运目标蛋白到植物体内的表达载体及其应用 | CN201610899180.0 | 2016-10-14 | CN106636080B | 2019-03-26 | 刘俊; 余永旗 |
| 本发明公开了一种利用水稻白叶枯病菌Ⅲ型分泌系统转运目标蛋白到植物体内的表达载体及其应用。本发明保护一种特异DNA分子，依次包括如下区段：区段甲、区段乙和区段丙；所述区段甲为HrcC启动子；所述区段乙为TAL信号肽；所述区段丙为转录终止序列。含有以上所述特异DNA分子的重组载体也属于本发明的保护范围。本发明还保护以上任一所述重组载体的应用，为表达目的蛋白并将其转运至植物细胞。本发明可用于鉴定待测蛋白是否为效应蛋白。本发明提供了一种简单易行且可批量操作的方法，该方法可将分泌蛋白强制分泌到植物体内，从而筛选出有重大生物学意义的效应蛋白。该发明对于植物病原菌致病机制的研究起到了一定的促进作用。 | ||||||
| 62 | 在植物中控制关键乙烯激素信号传导途径蛋白的目标周转率以调节乙烯敏感性 | CN201080010816.2 | 2010-03-02 | CN102361976A | 2012-02-22 | J·L·罗西尚 |
| 一种在植物细胞中可控地表达乙烯应答的基因表达系统，其包括激活盒，该激活盒包括识别效应元件的DNA结合域；蜕皮激素受体配体结合域；和激活域；以及靶标盒，该靶标盒包括诱导性启动子，其包含操作性相连的效应元件和对激活域起反应的最小启动子。该诱导性启动子控制编码所选调控蛋白的核酸序列的表达，从而改变植物中某种信号蛋白的乙烯敏感性。在植物细胞中，在诱导组合物存在时激活盒和靶标盒的组件之间的相互作用能提高所选调控蛋白的表达，进而降低信号蛋白在植物中的表达和蓄积，从而降低植物细胞的乙烯敏感性。可通过施加诱导组合物的时机、浓度和持续时间来控制该调控蛋白表达的增加(特别是在存在乙烯时)。提供在诱导组合物存在时能控制乙烯敏感性的转基因植物的细胞、组织、器官和完整植株。可控制这种转基因植物和组织的乙烯敏感性和/或乙烯生产以操纵熟化、开花、枯萎及植物的其他乙烯敏感性功能。 | ||||||
| 63 | 抗昆虫的转基因植物以及用于改善δ－内毒素抵抗目标昆虫活性的方法 | CN200710141972.2 | 1998-12-17 | CN101172999A | 2008-05-07 | L·恩格利斯; S·M·布鲁索克; T·M·马尔瓦; J·W·布赖森; C·A·库勒斯扎; F·S·瓦尔特斯; S·L·斯拉廷; M·A·冯特尔施; C·罗马诺 |
| 公开了一种增加苏云金杆菌δ－内毒素抗鞘翅目昆虫的活力的方法，同时还公开了突变编码该多肽的核酸以及在表达该基因的转基因植物中提高昆虫抗性的方法。 | ||||||
| 64 | TARGETED GENOME MODIFICATIONS IN PLANTS | PCT/EP2008/056770 | 2008-06-02 | WO2008145757A1 | 2008-12-04 | ANGENON, Geert; VERWEIRE, Dimitri; VAN EX, Frédéric |
The invention provides materials and methods for performing targeted genome modification in plants and plant cells. More particularly, the invention allows to present a targeting cassette preferentially in specific cell types and/or in cells at particular developmental or cell division stages, which allows for more efficient gene targeting. |
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| 65 | TARGETED GENOME ENGINEERING IN PLANTS | PCT/EP2013/058264 | 2013-04-22 | WO2013160230A1 | 2013-10-31 | D'HALLUIN, Katelijn |
Improved methods and means are provided to modify in a targeted manner the genome of a plant cell or plant at a predefined site via bacterial transformation. |
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| 66 | Objective plant raising sod and objective plant raising method of construction | JP2004238997 | 2004-08-19 | JP2006055045A | 2006-03-02 | YANAGIDA TOMOTAKA; KO YOSHU |
| PROBLEM TO BE SOLVED: To provide objective plant raising sod intended for solving such problems that in the case of greening a land using plants excluding lawn, the land is in a bare land condition during until the completion of a greened land and settling the bare land condition of an object land to be greened just after greening, and making a greened land by objective plants through changing lawn to objective plants, spending a few months to a few years, and to provide an objective plant raising method of construction. SOLUTION: The objective plant raising sod is formed by sowing or planting the seeds or part of the plant body of an objective plant on sod of lawn. Objective plant raising base sod is formed from sod of lawn of natural turf, Zoysia matrella and Tifton. The objective plant raising method of construction for forming the objective plant raising sod comprises sowing the seeds of an objective plant or scattering part of the plant body on a part stuck with sod of lawn or the soil layer of the lower part of sod. COPYRIGHT: (C)2006,JPO&NCIPI | ||||||
| 67 | 공변세포에의 목적 단백질 특이적 발현용 프로모터 | KR1020100125797 | 2010-12-09 | KR1020120064521A | 2012-06-19 | 김범기; 변명옥; 김동헌; 윤인선; 김둘이; 조혜선; 박종범; 김동균; 이효숙; 김현미; 황현식 |
| PURPOSE: A promoter which is expressed in guard cells and a recombinant expression vector containing the same are provided to enhance various environmental stress resistance. CONSTITUTION: A promoter for target protein-specific expression in plant leaves has a base of sequence number 1. The part of the plant leaves is guard cells. A recombinant expression vector contains a promoter for target protein-specific expression and a structural gene encoding the target protein. A transformant prepared by the expression vector is E.coli, Bacillus subtilis, Streptomyces, Pseudomonas, Proteus mirabilis, Staphylococcus, or Agrobacterium tumefaciens. | ||||||
| 68 | 목적 유전자로 표면개질된 임플란트 및 이의 제조방법 | KR1020140079877 | 2014-06-27 | KR1020160001401A | 2016-01-06 | 박형호; 이민호; 이호근; 이영희; 김가람 |
| 본발명은목적유전자, 특히염증억제용 PPARγ유전자또는골생성증진용 c-Myb 유전자로표면개질된임플란트및 이의제조방법에관한것이다. 본발명에따른표면개질된임플란트는목적유전자, 특히염증억제용 PPARγ유전자또는골생성증진용 c-Myb 유전자를임플란트표면에코팅함으로써, 경제적으로염증을억제하고골형성을증진하여치유기간을단축하고자가치유가가능하게하는효과가있다. | ||||||
| 69 | 공변세포 특이적 목적 단백질 발현용 프로모터 | KR1020120092494 | 2012-08-23 | KR1020140025915A | 2014-03-05 | 김범기; 변명옥; 권택윤; 윤인선; 김둘이; 김진애; 강현중; 황현식 |
| The present invention relates to an SLAC1 promoter specifically expressing target protein from leaves of a plant, a recombinant expression vector including the same, and a transformant using the same, more specifically to a promoter specifically expressing in guard cells of mono-cotyledonous plants, a recombinant expression vector including the same, and a transformant using the same. The SLAC1 promoter of the present invention controls the specific expression of the target protein in the guard cells of the mono-cotyledonous plants, thereby being practically used for the specific expression of genes which are useful for the growth and development of the plants or the improvement of stress resistance against various environmental factors, in the guard cells. | ||||||
| 70 | 植物体の種子における目的タンパク質の発現 | JP2015511174 | 2014-03-19 | JPWO2014167968A1 | 2017-02-16 | 雅武 金井; 西村 幹夫; 幹夫 西村; 健志 山田 |
| 植物体の種子における目的タンパク質の増大に寄与するDNA材料を新たに見出したので、これらの利用を開示する。この目的のために、本開示では、植物体の種子において目的タンパク質の発現を促進する、下記(a)〜(d)のいずれかに記載のDNAを植物体に導入する。(a)配列番号1、2、3又は4で表される塩基配列を有するDNA;(b)配列番号1、2、3又は4で表される塩基配列と相補的な塩基配列からなるDNAとストリンジェントな条件下でハイブリダイズするDNA;(c)配列番号1、2、3又は4で表される塩基配列と90%以上の同一性の塩基配列を有するDNA;及び(d)配列番号1、2、3又は4で表される塩基配列において1又は複数個の塩基の置換、欠失、挿入又は付加を有する塩基配列からなるDNA | ||||||
| 71 | USE OF PLANT LECTINS TO TARGET LEUKOCYTES | PCT/EP2012/058955 | 2012-05-14 | WO2012156376A1 | 2012-11-22 | LAVELLE, Edward; DAVITT, Christopher; MISSTEAR, Karen; RUANE, Darren; MCNEELA, Edel |
The present invention provides compositions and methods for targeting an antigen to leukocytes, delivering an antigen to leukocytes, increasing antigen uptake by leukocytes, and/or enhancing an immune response. In some embodiments, compositions and methods of the present invention comprise a conjugate comprising an antigen and a plant lectin or a mimetic thereof. |
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| 72 | IDENTIFICATION AND USE OF TARGET GENES FOR CONTROL OF PLANT PARASITIC NEMATODES | PCT/US2007061932 | 2007-02-09 | WO2007095469A8 | 2008-09-04 | BOUKHAROV ANDREY A; DU ZIJIN; GUO LIANG; HRESKO MICHELLE C; KOVALIC DAVID K; LI ZHAOLONG; LU MAOLONG; MCCARTER JAMES P; MILLER NANCY M; VAUDIN MARK; WILLIAMS DERYCK J; WU WEI |
| The invention relates to identifying and evaluating target coding sequences for control of plant parasitic nematodes by inhibiting one or more biological functions, and their use. The invention provides methods and compositions for identification of such sequences and for the control of a plant-parasitic nematode population. By feeding one or more recombinant double stranded RNA molecules provided by the invention to the nematode, a reduction in disease may be obtained through suppression of nematode gene expression. The invention is also directed to methods for making transgenic plants that express the double stranded RNA molecules, and the plant cells and plants obtained thereby. | ||||||
| 73 | IMPROVED TARGETED DNA INSERTION IN PLANTS | PCT/EP2004/013122 | 2004-11-17 | WO2005049842A2 | 2005-06-02 | D'HALLUIN, Kathleen; VANDERSTRAETEN, Chantal; RUITER, Rene |
Methods and means are provided to improve targeted DNA insertion in plants using rare-cleaving "double stranded break" inducing enzymes. Also provided are improved I-SceI encoding nucleotide sequences. |
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| 74 | PROCESSES AND VECTORS FOR PRODUCING TRANSGENIC PLANTS | PCT/EP2001/013662 | 2001-11-23 | WO02046438A2 | 2002-06-13 | |
| This invention describes a process for gene expression in plants utilizing translational vectors. Said translational vectors cause a gene of interest to be stably integrated into a transcriptionally active host genomic DNA such that the transcription the gene of interest is controlled by a promoter of the host organism. Said translational vectors are preferably based on internal ribosome entry site (IRES) elements that are of plant origin, plant viral origin, synthetic origin or are isolated from other organisms. | ||||||
| 75 | TISSUE-SPECIFIC EXPRESSION OF TARGET GENES IN PLANTS | PCT/CA0100066 | 2001-01-29 | WO0155433A3 | 2002-04-18 | GOOD ALLEN G |
| Methods of producing plants having tissue-specific expression of one or more target genes are taught, as are methods of producing plants having tissue-specific and environmental or developmentally-regulated expression of one or more target genes. A genetic construct is taught which contains a nitrogen assimilation and/or metabolism gene and a promoter for the gene which is inducible under conditions where it would be beneficial to take up, store or use nitrogen. The promoter can be, for example, induced by the presence of nitrate or other form of nitrogen and will be induced by application of a nitrogenous fertilizer. The stress inducible promoter btg-26 is also taught. Promoter btg-26 is isolated from the Brassica turgor gene - 26 and exhibits osmotic-stress induced and tissue-specific expression of an operatively linked gene. The present invention also provides for methods to produce plants with increased biomass and seed yield. | ||||||
| 76 | 植物体の種子における目的タンパク質の発現 | PCT/JP2014/057567 | 2014-03-19 | WO2014167968A1 | 2014-10-16 | 金井 雅武; 西村 幹夫; 山田 健志 |
植物体の種子における目的タンパク質の増大に寄与するＤＮＡ材料を新たに見出したので、これらの利用を開示する。この目的のために、本開示では、植物体の種子において目的タンパク質の発現を促進する、下記（ａ）～（ｄ）のいずれかに記載のＤＮＡを植物体に導入する。 （ａ）配列番号１、２、３又は４で表される塩基配列を有するＤＮＡ； （ｂ）配列番号１、２、３又は４で表される塩基配列と相補的な塩基配列からなるＤＮＡとストリンジェントな条件下でハイブリダイズするＤＮＡ； （ｃ）配列番号１、２、３又は４で表される塩基配列と９０％以上の同一性の塩基配列を有するＤＮＡ；及び （ｄ）配列番号１、２、３又は４で表される塩基配列において１又は複数個の塩基の置換、欠失、挿入又は付加を有する塩基配列からなるＤＮＡ |
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| 77 | OBJEKTBASIERTE KONFIGURATION EINER PROZESS- UND/ODER FERTIGUNGSANLAGE | PCT/EP2013/056709 | 2013-03-28 | WO2014154281A1 | 2014-10-02 | BURMEISTER, Sören |
Die Erfindung betrifft eine Vorrichtung (20) zum Konfigurieren einer Prozess- und/oder Fertigungsanlage (10). Konfigurationsobjekte (36, 38, 40), durch welche jeweils ein Parametersatz (42, 44, 46) zumindest einer Anlagenkomponente (14, 16, 18) zum Festlegen eines Teils des Betriebsablaufs bereitgestellt ist, werden hierzu zu einem Objektmodell verknüpft. Das Objektmodell soll für eine flexiblere Konfigurationsmöglichkeit der Anlage erweitert werden. Hierzu ist a) ein Skriptinterpreter (28) der Vorrichtung (20) dazu ausgelegt, durch Ausführen von Verknüpfungsanweisungen (66) eines Benutzerskripts (64) frei vom Benutzer definierbare Abhängigkeit zwischen den Parametersätzen (42, 44, 46) von zumindest zwei der Konfigurationsobjekte ((36, 38, 40)) zu erzeugen, und b) eine Analyseeinrichtung (30) die Vorrichtung (20) dazu ausgelegt, anhand der Verknüpfungsanweisungen (66) zu ermitteln, zwischen welchen der Konfigurationsobjekte (36, 38, 40) eine skriptbasierte Verknüpfung (68, 70) bewirkt ist, und die ermittelten, skriptbasierten Verknüpfungen (68,70) ebenfalls graphisch anzuzeigen. |
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| 78 | METHOD AND APPARATUS FOR TREATMENT OF TARGETED PLANTS | PCT/US2011/058794 | 2011-11-01 | WO2012061393A1 | 2012-05-10 | KLINE, III, William, N.; SCHERER, Peter, N.; LANGSTON, Vernon |
An apparatus (100) for selectively treating plants is disclosed. The apparatus may include a frame (106) arranged to provide a plurality of horizontally spaced apart, non-overlapping treatment regions (120). The frame (106) may separate the plurality of horizontally spaced apart, non-overlapping treatment regions (120). The apparatus (100) may further include a plurality of sensors (130), each treatment region including at least one sensor which is arranged to monitor the respective treatment region for a plant to be treated. The apparatus (100) may further include a fluid delivery system including a plurality of application devices (134), each treatment region (120) including at least one application device (134). |
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| 79 | TARGETED MANIPULATION OF GENES IN PLANTS | PCT/US2003/006583 | 2003-03-03 | WO2003076574A2 | 2003-09-18 | MAHAJAN, Pramod, B.; KANNAN, Priya |
Methods and compositions for altering a polynucleotide of interest in a plant are provided. The methods involve introducing a template oligonucleotide into a plant cell, the template oligonucleotide comprises regions complementary to the plant polynucleotide, the complementary regions flank a non-complementary region, and the template oligonucleotide comprises a single-stranded nucleic acid comprising at least one cationic phosphoramidate internucleoside linkage. |
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| 80 | PROCESSES AND VECTORS FOR PRODUCING TRANSGENIC PLANTS | PCT/EP0114421 | 2001-12-07 | WO0246440A2 | 2002-06-13 | GLEBA YURI; KLIMYUK VICTOR; BENNING GREGOR; ELIBY SERIK |
| This invention describes a process for gene expression in plants utilizing translational vectors. Said translational vectors cause a gene of interest to be stably integrated into a transriptionally active host genomic DNA such that the transcription the gene of interest is controlled by a promoter of the host organism. Said translational vectors are preferably based on internal ribosome entry site (IRES) elements that are of plant origin, plant viral origin, synthetic origin or are isolated from other organisms. | ||||||
