SEQUENCING BY SYNTHESIS USING PULSE READ OPTICS专利检索- .专门适用于建立化合物库和筛选化合物库的成套装置专利检索查询-专利查询网

SEQUENCING BY SYNTHESIS USING PULSE READ OPTICS
申请号	EP15841893	申请日	2015-09-16	公开(公告)号	EP3194643A4	公开(公告)日	2018-04-25
申请人	IBIS BIOSCIENCES INC;			发明人	ESHOO MARK W; CLEMENS JOHN M; HAYDEN MARK A;
摘要	Provided herein are systems and methods for nucleic acid sequencing by synthesis in a plurality of wells using detectably labeled chain terminating nucleotides with photolabile blocking groups and pulses of photocleaving light. In certain embodiments, the systems and methods provides a plurality of deblock-scan cycles comprising an initial deblock time period followed by a scanning light period, wherein at least one of the following occurs in each deblock-scan cycle: 1) the deblock time period is shorter than the scan time period; 2) the deblock time period is only long enough to deblock the photolabile groups that are part of a primer in less than all of the plurality of wells; or 3) the deblock time period is between 25 and 150 mSec and the scan time is at least 200 mSec. Such shorter deblock time periods help prevent the addition of more than one nucleotide to the primer prior to scanning (e.g., accuracy is enhanced).
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