Microfluidic Devices And Methods Of Use In The Formation And Control Of Nanoreactors |
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| 申请号 | EP10196339.5 | 申请日 | 2006-06-01 | 公开(公告)号 | EP2363205A3 | 公开(公告)日 | 2014-06-04 |
| 申请人 | Raindance Technologies, Inc.; | 发明人 | LINK, Darren, R.; BOITARD, Laurent; BRANCIFORTE, Jeffrey; CHARLES, Yves; FEKE, Gilbert; LU, John, Q.; MARRAN, David; TABATABAI, Ahmadali; WEINER, Michael; HINZ, Wolfgang; ROTHBERG, Jonathan, M.; | ||||
| 摘要 | The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays and combinatorial chemistry. Such methods can include labeling a library of compounds by emulsifying aqueous solutions of the compounds and aqueous solutions of unique liquid labels on a microfluidic device, which includes a plurality of electrically addressable, channel bearing fluidic modules integrally arranged on a microfabricated substrate such that a continuous channel is provided for flow of immiscible fluids, whereby each compound is labeled with a unique liquid label, pooling the labeled emulsions, coalescing the labeled emulsions with emulsions containing a specific cell or enzyme, thereby forming a nanoreactor, screening the nanoreactors for a desirable reaction between the contents of the nanoreactor, and decoding the liquid label,; thereby identifying a single compound from a library of compounds. | ||||||
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