특이 결합쌍의 멤버를 제조하기 위한 방법 |
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| 申请号 | KR1019930700061 | 申请日 | 1991-07-10 | 公开(公告)号 | KR100222326B1 | 公开(公告)日 | 1999-10-01 |
| 申请人 | 메디뮨 리미티드; 메디칼 리서취 카운실; | 发明人 | 맥카퍼티,존; 포프,앤토니,리챠드; 죤슨,케빈,스튜어트; 호우겐붐,헨드리커스,리네루스,야코부스,메튜우스; 그리피스,앤드류,데이비드; 잭슨,로날드,헨리; 홀리거,카스퍼,필립; 막스,제임스,데이비드; 크락크슨,티모시,피어스; 키스웰,데이비드,존; 윈터,그레고리,폴; 보네트,티모시,피터; | ||||
| 摘要 | A member of a specific binding pair (sbp) is identified by expressing DNA encoding a genetically diverse population of such sbp members in recombinant host cells in which the sbp members are displayed.in functional form at the surface of a secreted recombinant genetic display package (rgdp) containing DNA encoding the sbp member or a polypeptide component thereof, by virtue of the sbp member or a polypeptide component thereof being expressed as a fusion with a capsid component of the rgdp. The displayed sbps may be selected by affinity with a complementary sbp member, and the DNA recovered from selected rgdps for expression of the selected sbp members. Antibody sbp members may be thus obtained, with the different chains thereof expressed, one fused to the capsid component and the other in free form for association with the fusion partner polypeptide. A phagemid may be used as an expression vector, with said capsid fusion helping to package the phagemid DNA. Using this method libraries of DNA encoding respective chains of such multimeric sbp members may be combined, thereby obtaining a much greater genetic diversity in the sbp members than could easily be obtained by conventional methods. | ||||||
| 权利要求 | |||||||
| 说明书全文 | |||||||
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