O-ANTIGEN CARBOHYDRATE CHAIN EXTENDED SALMONELLA PARATYPHI A AND USE THEREOF |
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| 申请号 | EP15867235 | 申请日 | 2015-09-07 | 公开(公告)号 | EP3231868A4 | 公开(公告)日 | 2018-05-23 |
| 申请人 | INST OF BIOTECHNOLOGY ACADEMY OF MILITARY MEDICAL SCIENCES CHINA; | 发明人 | WU JUN; SUN PENG; LIU BO; CHANG SHAOHONG; GONG XIN; WANG HENGLIANG; ZHU LI; PAN CHAO; FENG ERLING; | ||||
| 摘要 | The present invention discloses a Salmonella Paratyphi A with an O-antigen having an extended carbohydrate chain and uses thereof. The method comprises the following steps: inactivating an cld gene encoding an enzyme controlling chain length of O-antigen of a Salmonella paratyphi A strain to obtain a Salmonella paratyphi A with deletion of cld gene; allowing overexpression of cld LT2 gene encoding an enzyme controlling chain length of O-antigen of Salmonella typhimurium in Salmonella paratyphi A deficient in the cld gene encoding an enzyme controlling chain length of O-antigen, so as to extend carbohydrate chain length of O-antigen. Both of the Salmonella paratyphi A O-polysaccharide-recombinant fusion protein conjugate vaccines rCTB4573 3 -OPS Spty 50973 and rEPA4573-OPS Spty 50973 as prepared by using Salmonella Paratyphi A with an O-antigen having an extended carbohydrate chain can induce mice to generate specific antibodies against Salmonella paratyphi A, and their antibody titers are significantly improved. | ||||||
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