METHODS AND COMPOSITIONS FOR PCR |
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| 申请号 | PCT/US2008074432 | 申请日 | 2008-08-27 | 公开(公告)号 | WO2009029648A3 | 公开(公告)日 | 2009-04-30 |
| 申请人 | APPLERA CORP; XI LARRY L; PROSEN DENNIS E; NOVIKOV ALEXANDER A; | 发明人 | XI LARRY L; PROSEN DENNIS E; NOVIKOV ALEXANDER A; | ||||
| 摘要 | A modified thermostable Pol B DNA polymerase, produced by a reaction, under essentially aqueous conditions, of a thermostable Pol B DNA polymerase and a modifier reagent of Formula (I) wherein the reaction results in a thermally reversible inactivation of the thermostable Pol B DNA polymerase activity and the 3'-5' exonuclease activity, which polymerase is suitable for hot-start PCR. Also disclosed are the method for the modification, a polynucleic acid amplification method and PCR reaction mixture and kit comprising the modified thermostable Pol B DNA polymerase. | ||||||
| 权利要求 | |||||||
| 说明书全文 | |||||||
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