Methods And Compositions For PCR |
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| 申请号 | US15821651 | 申请日 | 2017-11-22 | 公开(公告)号 | US20180094250A1 | 公开(公告)日 | 2018-04-05 |
| 申请人 | Life Technologies Coroporation; | 发明人 | Lei (Larry) XI; Dennis PROSEN; Alexander NOVIKOV; | ||||
| 摘要 | A modified thermostable Pol B DNA polymerase, produced by a reaction, under essentially aqueous conditions, of a thermostable Pol B DNA polymerase and a modifier reagent of Formula I wherein the reaction results in a thermally reversible inactivation of the thermostable Pol B DNA polymerase activity and the 3′-5′ exonuclease activity, which polymerase is suitable for hot-start PCR. Also disclosed are the method for the modification, a polynucleic acid amplification method and PCR reaction mixture and kit comprising the modified thermostable Pol B DNA polymerase. | ||||||
| 权利要求 | |||||||
| 说明书全文 | |||||||
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