METHODS AND COMPOSITIONS FOR PCR |
|||||||
| 申请号 | EP08798774 | 申请日 | 2008-08-27 | 公开(公告)号 | EP2183380A4 | 公开(公告)日 | 2011-11-30 |
| 申请人 | LIFE TECHNOLOGIES CORP; | 发明人 | XI LARRY L; PROSEN DENNIS E; NOVIKOV ALEXANDER A; | ||||
| 摘要 | A modified thermostable Pol B DNA polymerase, produced by a reaction, under essentially aqueous conditions, of a thermostable Pol B DNA polymerase and a modifier reagent of Formula (I) wherein the reaction results in a thermally reversible inactivation of the thermostable Pol B DNA polymerase activity and the 3'-5' exonuclease activity, which polymerase is suitable for hot-start PCR. Also disclosed are the method for the modification, a polynucleic acid amplification method and PCR reaction mixture and kit comprising the modified thermostable Pol B DNA polymerase. | ||||||
| 权利要求 | |||||||
| 说明书全文 | |||||||
QQ群二维码
意见反馈
意见反馈