Using RNA-guided FokI Nucleases (RFNs) to Increase Specificity for RNA-Guided Genome Editing |
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| 申请号 | US16003973 | 申请日 | 2018-06-08 | 公开(公告)号 | US20180340189A1 | 公开(公告)日 | 2018-11-29 |
| 申请人 | The General Hospital Corporation; | 发明人 | J. Keith Joung; Shengdar Tsai; | ||||
| 摘要 | Many studies have shown that CRISPR-Cas nucleases can tolerate up to five mismatches and still cleave; it is hard to predict the effects of any given single or combination of mismatches on activity. Taken together, these nucleases can show significant off-target effects but it can be challenging to predict these sites. Described herein are methods for increasing the specificity of genome editing using the CRISPR/Cas system, e.g., using RNA-guided Foki Nucleases (RFNs), e.g., FokI-Cas9 or Foki-dCas9-based fusion proteins. | ||||||
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