METHODS OF SPECIFICALLY LABELING NUCLEIC ACIDS USING CRISPR/CAS专利检索- 作用于酯键的水解酶专利检索查询-专利查询网

METHODS OF SPECIFICALLY LABELING NUCLEIC ACIDS USING CRISPR/CAS
申请号	US15787319	申请日	2017-10-18	公开(公告)号	US20180105867A1	公开(公告)日	2018-04-19
申请人	Drexel University; The Wistar Institute of Anatomy and Biology; Temple University - of the Commonwealth System of Higher Education;			发明人	Ming Xiao; Harold C. Riethman; Wenhui Hu; Jennifer McCaffrey;
摘要	Provided herein are methods of detecting a target nucleic acid sequence. In one embodiment, the method includes contacting genomic DNA with a guide RNA having a portion complementary to the target sequence in the genomic DNA and with Cas9 nickase to produce a single-strand break in the genomic DNA at a specific location adjacent to the target sequence. The method further includes contacting the nicked DNA with a polymerase and fluorescently labeled nucleotide. The fluorescently labeled nucleotide is incorporated into the nicked DNA at the specific location and the target nucleic acid sequence is detected via fluorescent label.
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