The present invention provides a miniaturizable tool that can analyze a target in a sample by a simple operation and an analysis method using the tool. An analysis chip is a syringe-type chip including: a syringe main body having an openable and closable tip; and a piston to be inserted into the syringe main body, the piston being a hollow body having a translucent closed-end at a side to be inserted into the syringe main body and an open-end at the other side. Analysis device includes a main body case that is a housing, the housing including: an insertion opening into which the syringe-type chip is to be inserted; and a void in communication with the insertion opening, wherein an opposite end of the insertion opening in an axial direction is a bottom; a heating unit that heats the syringe-type chip; a light source unit that emits light to the syringe-type chip; and a detection unit that is a columnar body that can be inserted into the hollow body of the piston and disposed at the bottom of the housing in the axial direction. In use, the syringe-type chip is inserted into the analysis device from a side of the piston and the detection unit of the analysis device is inserted into the piston of the syringe-type chip.

To provide a check-valve not requiring any motive power sources such as a pump and a piezoelectric element for directly pressurizing and depressurizing for a valve chamber from above, capable of making its size compact by a simple structure and further capable of stopping positively a reverse flow while flowing smoothly a specimen and a reagent.

A check-valve (1) comprises: a thin sheet (20) and a thick sheet (40); flow paths (21), (41) for flowing fluid which are formed by penetrating the thin sheet (20) and the thick sheet (40); a flow-in chamber (22) and a flow-out chamber (42) which are connected to the flow paths (21), (41); a partition sheet (30) which is bonded to the thin sheet (20) and the thick sheet (40) while being sandwiched therebetween, and has a flexible inner flange (32) which projects in cavities of the flow-in chamber (22) and the flow-out chamber (42), and does not close the flow-out valve chamber (42) by flexing toward the flow-out valve chamber (42) in a normal flow, and closes the flow-in valve chamber (22) by flexing toward the flow-in valve chamber (22) in a reverse flow; and a through-pass hole (31) which is opened at the partition sheet (30) and connects the both valve chambers (22), (42).

A fluidic device includes:

a first flow path in which two or more solutions are mixed; and

a second circulation flow path in which a solution mixed in the first flow path is circulated and which has a capture part configured to capture a sample substance included in the solution and/or a detection part configured to detect a sample substance included in the solution.

A microfluidic device (1) includes a flow path (100) through which a reaction solution (200) flows, and an introducing portion (120) for introducing the reaction solution (200) into the flow path (100), wherein the flow path (100) passes alternately and repeatedly several times through a first temperature region and a second temperature region having different predetermined temperatures, and the flow path (100) includes the repeating unit portions passing through the first temperature region and the second temperature region, the repeating unit portions having decreasing lengths as the repeating unit portions are located farther away from the introducing portion (120).

A liquid handling device has an accommodation part for accommodating a liquid, two or more flow paths each opening to a lower part of a side wall surface of the accommodation part, and a liquid movement suppression part that is disposed in the lower part of the side wall between the openings of two of the flow paths that are adjacent to each other and slows or stops the movement of the liquid along the corner formed by the lower surface of the accommodation part and the side wall surface.

The present invention provides a kit or device for the detection of biliary tract cancer, and a method for detecting biliary tract cancer. The present invention relates to a kit or device for the detection of biliary tract cancer, comprising a nucleic acid capable of specifically binding to miRNA in a sample of a subject, and a method for detecting biliary tract cancer, comprising measuring the miRNA in vitro.

The present invention provides a tool that can analyze a target in a sample by a simple operation and can be downsized and an analysis method using the tool. By inserting an analysis chip into an analysis device, a reaction of a sample and a reagent is analyzed. The analysis chip includes a parallel flow channel in which plural reaction flow channels are connected in parallel, and the axial direction of the parallel flow channel is a direction of inserting the analysis chip into the analysis device. The analysis device includes a main body case being a housing, including an insertion opening into which the analysis chip is to be inserted; and a void; a heating unit that causes a sample to thermally react with a reagent, the heating unit being disposed in the void at at least one of inner surfaces so as to face a parallel surface of the parallel flow channel of the analysis chip; a light source unit that emits light to the analysis chip, the light source unit being disposed in the void at least above or below a reaction flow channel located at at least one end of the parallel flow channel of the analysis chip and extended along the axial direction of the parallel flow channel; and a plurality of detection units that detect the thermal reactions, each detection unit being disposed in the void at a downstream side end in the insertion direction of the analysis chip so as to correspond to each reaction flow channel of the analysis chip.

Provided is a method for producing a microchannel including an approximately circular cross section with neither a joined surface nor an inlet in a smaller number of steps than has been conventional. The method for producing a microchannel includes the steps of forming a layer of an uncured curable resin on a substrate, inserting into the curable resin a needle body that can inject a liquid, injecting a liquid in a tubular shape into the curable resin via the needle body while moving the needle body, extracting the needle body from the curable resin, and curing the curable resin to form a channel in a tubular region injected with the liquid.

A sensor control device (12) for connection to an oxygen sensor (10) including a sensing element (11) that measures oxygen concentration in an intake atmosphere of an internal combustion engine (40) and a heater (17) that heats the sensing element, including a detection unit (13) that detects an output signal (IP) corresponding to the oxygen concentration output from the sensing element and a calculation unit (15) that calculates a compensation coefficient of the output signal that is used when calculating the oxygen concentration. The calculation unit collects compensation information used in calculating the compensation coefficient when the internal combustion engine is in operation and in a specific operation state in which the oxygen concentration in the intake atmosphere is subject to estimation. Also disclosed is a sensor control system (1) which includes an oxygen sensor (10) and the sensor control device (12).