| 序号 | 专利名 | 申请号 | 申请日 | 公开（公告）号 | 公开（公告）日 | 发明人 |
|---|---|---|---|---|---|---|
| 1 | 用于在体内进行蛋白质的选择性进化的方法 | CN200680028628.6 | 2006-08-07 | CN101238212A | 2008-08-06 | M·里斯 |
| 本发明涉及用于制备蛋白质变体的方法，所述蛋白质变体与起始蛋白质相比具有改善的特性，其中所述变体借助于体内进化方法而获得。 | ||||||
| 2 | 用于在体内进行蛋白质的选择性进化的方法 | CN200680032319.6 | 2006-08-07 | CN101258244A | 2008-09-03 | M·里斯 |
| 本发明涉及在体外进化方法中产生蛋白质的变体，所述方法包括下列步骤：(A)提供体外表达系统，其包含：(i)编码待改变的蛋白质Y的核酸序列S；(ii)能够结合蛋白质Y和/或至少一种其变体Y’的靶分子X；(iii)能够转录核酸序列S的RNA聚合酶(Pol)；(iv)能够反转录核酸S的转录物的逆转录酶(RT)，其中靶分子X与Pol偶联而蛋白质Y与RT偶联，或者靶分子X与RT偶联而蛋白质Y与Pol偶联；(B)在一定的条件下温育来自(A)的体外表达系统，所述条件使得能够进行转录、反转录和翻译从而形成蛋白质Y的变体Y’和编码其的核酸序列S’，并且有利于形成具有改善的对靶分子X的结合特性的变体Y’；(C)分离和任选地表征具有改善的结合X的结合特性的那些变体Y’，和/或分离编码Y’的核酸序列变体S’。 | ||||||
| 3 | 用于在体内进行蛋白质的选择性进化的方法 | CN200680032319.6 | 2006-08-07 | CN101258244B | 2012-02-15 | M·里斯 |
| 本发明涉及在体外进化方法中产生蛋白质的变体，所述方法包括下列步骤：(A)提供体外表达系统，其包含：(i)编码待改变的蛋白质Y的核酸序列S；(ii)能够结合蛋白质Y和/或至少一种其变体Y’的靶分子X，(iii)能够转录核酸序列S的RNA聚合酶(Pol)，(iv)能够反转录核酸S的转录物的逆转录酶(RT)，其中靶分子X与Pol偶联而蛋白质Y与RT偶联，或者靶分子X与RT偶联而蛋白质Y与Pol偶联；(B)在一定的条件下温育来自(A)的体外表达系统，所述条件使得能够进行转录、反转录和翻译从而形成蛋白质Y的变体Y′和编码其的核酸序列S′，并且有利于形成具有改善的对靶分子X的结合特性的变体Y′；(C)分离和任选地表征具有改善的结合X的结合特性的那些变体Y′，和/或分离编码Y′的核酸序列变体S′。 | ||||||
| 4 | 通过进化和理性设计的组合获得的用于产生1，2-丙二醇的新微生物 | CN200880009616.8 | 2008-03-21 | CN101641446A | 2010-02-03 | 菲利普·索凯尔; 弗朗索瓦·沃尔克; 雷纳·菲格 |
| 本发明涉及将进化和理性设计组合用于制备可从碳源产生1，2-丙二醇的微生物菌株的新方法。所述方法包括：-在适当的生长培养基中，在选择压力下培养初始菌株，所述初始细菌菌株包含的tpiA基因表达削弱，并且丙酮醛转化成乳酸中涉及的至少一个基因的表达削弱，以促进所述初始菌株中的进化，-然后选择和分离1，2-丙二醇生产速率增加的进化菌株。-然后在进化菌株中重构功能性tpiA基因。本发明还涉及进化菌株，如获得的菌株，其可以另外进行遗传修饰以优化从碳源到1，2-丙二醇的方法，不产生副产物，得到最优的可能得率。 | ||||||
| 5 | Method for obtaining new form of life | JP2009281641 | 2009-12-11 | JP2010051332A | 2010-03-11 | MUTZEL RUPPERT; MARLIERE PHILIPPE; MAZEL DIDIER |
| <P>PROBLEM TO BE SOLVED: To provide a method for generating a new form of life. <P>SOLUTION: The method for generating the new form of life comprises the steps of: (a) irreversible alteration of the genome of a microbial clone; (b) cultivation of a vast population of microbial cells originating from the altered clone obtained in the step (a) during numerous generations under conditions allowing selection for a higher and stable proliferation rate; and (c) isolation of descendant clones within the cultivated population of the step (b) still bearing the alteration of the step (a). <P>COPYRIGHT: (C)2010,JPO&INPIT | ||||||
| 6 | Way to get a new life form | JP2003510814 | 2002-07-08 | JP2004533265A | 2004-11-04 | マッツェル，ディディエ; マルリエール，フィリップ; ムッツェル，ルペルト |
| 本発明は、新規な生命形態を生成するための方法であって、以下のステップ：
ａ）微生物クローンのゲノムの不可逆的な変更； ｂ）より高く安定な増殖速度を選択することの可能な条件下における数多くの世代の間にステップａ）において得られた変更されたクローンに由来する微生物の大きな集団の培養； ｃ）ステップｂ）の培養集団内の子孫クローンであって、ステップａ）の変更をまだ行っているものの単離。 を含む前記方法に関する。 |
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| 7 | Method of invitro molecular evolution of antibody function | JP2001572965 | 2001-04-04 | JP2003529368A | 2003-10-07 | エスキル・ソーデルリンド; マッツ・オーリン; ローランド・カールソン |
| (57)【要約】 選択されたフレームワーク（“マスターフレームワーク”）内に位置する相補性決定領域（ＣＤＲ）を含む抗体可変ドメインをコードするポリヌクレオチド配列を生成する方法であって、ａ）一以上のＣＤＲと、関連したフレームワーク領域（“オリジナルフレームワーク”）とをコードする少なくとも一つの核酸分子を準備する工程；ｂ）増幅プライマーとして一以上の対のオリゴヌクレオチドを用いて、工程（ａ）の核酸分子の少なくとも一つのＣＤＲコード部を増幅する工程；およびｃ）工程（ｂ）で生成された、増幅されたＣＤＲをコードするヌクレオチド配列と、前記マスターフレームワークをコードするヌクレオチド配列とを組み合わせて、抗体可変ドメインをコードするポリヌクレオチド配列を組み立てる工程を含む。 | ||||||
| 8 | 関心対象のタンパク質の細胞表面ディスプレイ、スクリーニング、および産生 | JP2010500980 | 2008-03-26 | JP5754135B2 | 2015-07-29 | レイクストロー, ジェームス エー.; レトフスキー, スタン アイ.; リッポウ, シャウン エム. |
| 9 | Cell surface display, screening and production of protein of interest | JP2013170132 | 2013-08-20 | JP2013233157A | 2013-11-21 | RAKESTRAW JAMES A; LETOVSKY STAN I; LIPPOW SCHAUN M |
| PROBLEM TO BE SOLVED: To solve the problem that current screening methods are limited by the size of the libraries, the lengths and complexities of polypeptides and available functional assays.SOLUTION: Aspects of the invention provide compositions and methods for displaying engineered polypeptides on a cell surface. According to aspects of the invention, immobilized polypeptides can be secreted to identify one or more variants having one or more functional or structural properties of interest. Aspects of the invention provide compositions and methods for producing engineered protein or protein variants having a functional or a structural property of interest. Aspects of the invention include methods of expressing and displaying engineered proteins on host cells. Aspects of the invention include nucleic acid constructs, expressed proteins, host cells, and/or isolated proteins. | ||||||
| 10 | Method of invitro molecular evolution of antibody function | JP2001572965 | 2001-04-04 | JP4842490B2 | 2011-12-21 | エスキル・ソーデルリンド; マッツ・オーリン; ローランド・カールソン |
| 11 | Generation of libraries of soluble random polypeptide linked to mRNA | JP2009549303 | 2008-02-12 | JP2010517583A | 2010-05-27 | リチャード ビー． ウィリアムズ、 |
| 可溶性ランダムポリペプチドのライブラリーを作製するための方法および組成物が提供される。 この方法では、ポリペプチド中の親水性残基の分率が、ポリペプチド構築物の溶解度を維持するために制御される。 | ||||||
| 12 | A method for the evolution adapted to the continuous purpose of the protein in the invivo | JP2008525457 | 2006-08-07 | JP2009504144A | 2009-02-05 | リス ミヒャエル |
| 本発明は、出発タンパク質と比較して改善された特性を有するタンパク質の変異体の製造方法であって、この変異体がｉｎ ｖｉｖｏ進化方法により得られる製造方法に関する。 | ||||||
| 13 | 진화 및 합리적 설계의 조합에 의해 수득된 １,２―프로판디올의 생산을 위한 신규한 미생물 | KR1020097022123 | 2008-03-21 | KR1020100015810A | 2010-02-12 | 수카유,필리프; 보엘케르,프랑소와; 피게,라이너 |
| The present invention concerns a new method combining evolution and rational design for the preparation of a strain of micro-organism for the production of 1,2-propanediol from a carbon source. The said method comprises:-growing an initial strain under selection pressure in an appropriate growth medium, said initial bacterial strain comprising an attenuation of the expression of theA gene and an attenuation the expression of at least one gene involved in the conversion of methylglyoxal to lactate, in order to promote evolution in said initial strain,-then selecting and isolating the evolved strain having an increased 1,2 propanediol production rate,-then reconstructing a functionalAgene in the evolved strain; The present invention also concerns the evolved strain such as obtained, that may be furthermore genetically modified in order to optimize the conversion of a carbon source into 1,2-propanediol without by-products and with the best possible yield. | ||||||
| 14 | 진화 및 합리적 설계의 조합에 의해 수득된 １,２―프로판디올의 생산을 위한 신규한 미생물 | KR1020097022123 | 2008-03-21 | KR101528943B1 | 2015-06-15 | 수카유,필리프; 보엘케르,프랑소와; 피게,라이너 |
| 탄소공급원으로부터 1,2-프로판디올의생산을위한미생물균주를제조하기위해, 진화및 합리적인설계를조합한신규한방법에관한것이다. 상기방법은 - 최초균주의진화를촉진시키기위해, tpiA 유전자의발현및 메틸글리옥살을락테이트로전환시키는데관여하는 1종이상의유전자의발현을감쇠시킨최초박테리아균주를적절한성장배지에서선택압력하에성장시키는단계, - 1,2 프로판디올생산율이증가된진화된균주를선택및 단리하는단계, 및 - 상기진화된균주에서기능성 tpiA 유전자를재구축하는단계를포함한다. 본발명은또한, 부산물없이최상의가능한수율로탄소공급원의 1,2-프로판디올로의전환을최적화하기위해추가로유전적으로변형될수 있는, 상기와같이얻어진진화된균주에관한것이다. | ||||||
| 15 | IMPROVED METHODS FOR MAKING AND USING POLYNUCLEOTIDE SEQUENCES IN THE SYNTHESIS OF ALKALOID COMPOUNDS | EP15785253 | 2015-04-27 | EP3137618A4 | 2017-12-13 | FACCHINI PETER JAMES |
| Novel methods that may be used for the manufacture of plant alkaloid compounds and novel polynucleotide compounds are provided. The plant alkaloid compounds are useful as medicinal compounds. | ||||||
| 16 | METHOD FOR MODULATING THE EVOLUTION OF A POLYPEPTIDE ENCODED BY A NUCLEIC ACID SEQUENCE | EP05850658.5 | 2005-09-19 | EP1799823B1 | 2009-11-25 | MAZEL, Didier; CAMBRAY, Guillaume |
| 17 | GENERATION OF LIBRARY OF SOLUBLE RANDOM POLYPEPTIDES LINKED TO mRNA | EP08729681.0 | 2008-02-12 | EP2118344A2 | 2009-11-18 | WILLIAMS, Richard, B. |
| Methods and compositions are provided for producing libraries of soluble random polypeptides. In the methods, the fraction of hydrophilic residues in the polypeptide is controlled so as to maintain the solubility of the polypeptide constructs. | ||||||
| 18 | STRUCTURE-ACTIVITY RELATIONSHIPS | EP08826299.3 | 2008-02-12 | EP2118281A2 | 2009-11-18 | MUNDORFF, Emily; DAVIS, Simon, Christopher; HUISMAN, W., Gjalt; KREBBER, Anke; GRATE, H., John |
| The present disclosure relates to compositions and methods for screening a plurality of polypeptide variants. | ||||||
| 19 | A METHOD FOR IN VITRO MOLECULAR EVOLUTION OF ANTIBODY FUNCTION | EP01925538.9 | 2001-04-04 | EP1268801A2 | 2003-01-02 | OHLIN, Mats; SODERLIND, Eskil; CARLSSON, Roland |
| The present invention provides a method for producing a polynucleotide sequence encoding an antibody variable domain, the variable domain comprising complementarity-determining regions (CDRs) located within a selected framework (the 'master framework'), the method comprising the steps of (a) providing at least one nucleic acid molecule encoding one or more CDRs and associated framework regions (the 'original framework'), (b) amplifying at least one CDR-encoding portion of the nucleic acid molecule(s) of step (a) using one or more pairs of oligonucleotides as amplification primers and (c) assembling a polynucleotide sequence encoding an antibody variable domain by combining the amplified CDR-encoding nucleotide sequences produced in step (b) with nucleotide sequences encoding said master framework, wherein the oligonucleotide primers of step (b) comprise nucleotide sequences which differ from the corresponding nucleotide sequences encoding said master framework. The invention further provides an antibody library, such as a phage display library, and methods of making the same. | ||||||
| 20 | Obtained by a combination of evolution and rational design, novel microorganisms for the production of 1,2-propanediol | JP2009554045 | 2008-03-21 | JP5570821B2 | 2014-08-13 | フィリップ、スカイユ; フランソワ、フェルケ; ライナー、フィゲ |
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