| 序号 | 专利名 | 申请号 | 申请日 | 公开（公告）号 | 公开（公告）日 | 发明人 |
|---|---|---|---|---|---|---|
| 1 | 原核生物中的糖基化蛋白表达 | CN200980107595.8 | 2009-01-05 | CN101960017B | 2017-06-09 | M·德利萨; C·瓜里诺; T·曼塞尔; A·费希尔 |
| 本发明涉及包含真核糖基转移酶活性的原核宿主细胞，其中所述真核糖基转移酶活性为真核多萜醇基连接的(dolichyl‑linked)UDP‑GIcNAc转移酶活性和真核甘露糖基转移酶活性。还公开了如下产生糖基化蛋白的方法：提供包含真核糖基转移酶活性的原核宿主细胞并且在有效产生糖基化蛋白的条件下培养所述原核宿主细胞。本发明的另一方面涉及如下用于筛选细菌或噬菌体的方法：在细菌的表面表达一种或多种聚糖，将标记附连在所述细菌表面或在源自所述细菌的噬菌体表面的一种或多种聚糖上，并以高通量形式分析所述标记。还公开了包含识别并结合天然抗原的Fv部分和在保守的天冬酰胺残基处被糖基化的Fc部分的糖基化抗体。 | ||||||
| 2 | 原核生物中的糖基化蛋白表达 | CN200980107595.8 | 2009-01-05 | CN101960017A | 2011-01-26 | M·德利萨; C·瓜里诺; T·曼塞尔; A·费希尔 |
| 本发明涉及包含真核糖基转移酶活性的原核宿主细胞，其中所述真核糖基转移酶活性为真核多萜醇基连接的(dolichyl-linked)UDP-GIcNAc转移酶活性和真核甘露糖基转移酶活性。还公开了如下产生糖基化蛋白的方法：提供包含真核糖基转移酶活性的原核宿主细胞并且在有效产生糖基化蛋白的条件下培养所述原核宿主细胞。本发明的另一方面涉及如下用于筛选细菌或噬菌体的方法：在细菌的表面表达一种或多种聚糖，将标记附连在所述细菌表面或在源自所述细菌的噬菌体表面的一种或多种聚糖上，并以高通量形式分析所述标记。还公开了包含识别并结合天然抗原的Fv部分和在保守的天冬酰胺残基处被糖基化的Fc部分的糖基化抗体。 | ||||||
| 3 | Glycosylated protein expression in prokaryotes | US14630308 | 2015-02-24 | US09512434B2 | 2016-12-06 | Matthew Delisa; Cassandra Guarino; Thomas Mansell; Adam Fisher |
| The present invention relates to a prokaryotic host cell comprising eukaryotic glycosyltransferase activity, where the eukaryotic glycosyltransferase activity is eukaryotic dolichyl-linked UDP-GlcNAc transferase activity and eukaryotic mannosyltransferase activity. Also disclosed is a method of producing a glycosylated protein by providing a prokaryotic host cell comprising the eukaryotic glycosyltransferase activity and culturing the prokaryotic host cell under conditions effective to produce a glycosylated protein. Another aspect of the present invention pertains to a method for screening bacteria or bacteriophages by expressing one or more glycans on the surface of a bacteria, attaching a label on the one or more glycans on the surface of the bacteria or on the surface of a bacteriophage derived from the bacteria, and analyzing the label in a high-throughput format. A glycosylated antibody comprising an Fv portion which recognizes and binds to a native antigen and an Fc portion which is glycosylated at a conserved asparagine residue is also disclosed. | ||||||
| 4 | GLYCOSYLATED PROTEIN EXPRESSION IN PROKARYOTES | US12811788 | 2009-01-05 | US20110039729A1 | 2011-02-17 | Matthew Delisa; Cassandra Guarino; Thomas Mansell; Adam Fisher |
| The present invention relates to a prokaryotic host cell comprising eukaryotic glycosyltransferase activity, where the eukaryotic glycosyltransferase activity is eukaryotic dolichyl-linked UDP-GlcNAc transferase activity and eukaryotic mannosyl-transferase activity. Also disclosed is a method of producing a glycosylated protein by providing a prokaryotic host cell comprising the eukaryotic glycosyltransferase activity and culturing the prokaryotic host cell under conditions effective to produce a glycosylated protein. Another aspect of the present invention pertains to a method for screening bacteria or bacteriophages by expressing one or more glycans on the surface of a bacteria, attaching a label on the one or more glycans on the surface of the bacteria or on the surface of a bacteriophage derived from the bacteria, and analyzing the label in a high-throughput format. A glycosylated antibody comprising an Fv portion which recognizes and binds to a native antigen and an Fc portion which is glycosylated at a conserved asparagine residue is also disclosed. | ||||||
| 5 | GLYCOSYLATED PROTEIN EXPRESSION IN PROKARYOTES | US14630308 | 2015-02-24 | US20150232861A1 | 2015-08-20 | Matthew DELISA; Cassandra GUARINO; Thomas MANSELL; Adam FISHER |
| The present invention relates to a prokaryotic host cell comprising eukaryotic glycosyltransferase activity, where the eukaryotic glycosyltransferase activity is eukaryotic dolichyl-linked UDP-GlcNAc transferase activity and eukaryotic mannosyltransferase activity. Also disclosed is a method of producing a glycosylated protein by providing a prokaryotic host cell comprising the eukaryotic glycosyltransferase activity and culturing the prokaryotic host cell under conditions effective to produce a glycosylated protein. Another aspect of the present invention pertains to a method for screening bacteria or bacteriophages by expressing one or more glycans on the surface of a bacteria, attaching a label on the one or more glycans on the surface of the bacteria or on the surface of a bacteriophage derived from the bacteria, and analyzing the label in a high-throughput format. A glycosylated antibody comprising an Fv portion which recognizes and binds to a native antigen and an Fc portion which is glycosylated at a conserved asparagine residue is also disclosed. | ||||||
| 6 | Glycosylated protein expression in prokaryotes | US12811788 | 2009-01-05 | US08999668B2 | 2015-04-07 | Matthew DeLisa; Cassandra Guarino; Thomas Mansell; Adam Fisher |
| The present invention relates to a prokaryotic host cell comprising eukaryotic glycosyltransferase activity, where the eukaryotic glycosyltransferase activity is eukaryotic dolichyl-linked UDP-GlcNAc transferase activity and eukaryotic mannosyl-transferase activity. Also disclosed is a method of producing a glycosylated protein by providing a prokaryotic host cell comprising the eukaryotic glycosyltransferase activity and culturing the prokaryotic host cell under conditions effective to produce a glycosylated protein. Another aspect of the present invention pertains to a method for screening bacteria or bacteriophages by expressing one or more glycans on the surface of a bacteria, attaching a label on the one or more glycans on the surface of the bacteria or on the surface of a bacteriophage derived from the bacteria, and analyzing the label in a high-throughput format. A glycosylated antibody comprising an Fv portion which recognizes and binds to a native antigen and an Fc portion which is glycosylated at a conserved asparagine residue is also disclosed. | ||||||
| 7 | PSEUDOGENES AND USES THEREOF | US13720411 | 2012-12-19 | US20130189679A1 | 2013-07-25 | Arul M. Chinnaiyan; Chandan Kumar-Sinha; Shanker Kalyana-Sundaram |
| The present invention relates to compositions and methods for cancer diagnosis, research and therapy, including but not limited to, cancer markers. In particular, the present invention relates to pseudogenes as diagnostic markers and clinical targets for prostate cancer. | ||||||
| 8 | Method of expression glycosylated proteins in prokaryotes | JP2010541578 | 2009-01-05 | JP2011508607A | 2011-03-17 | カサンドラ グァリーノ; マシュー デリーサ; アダム フィッシャー; トマス マンセル |
| 本発明は、真核生物のドリキル結合型UDP-GlcNAcトランスフェラーゼ活性および真核生物のマンノシルトランスフェラーゼ活性である真核生物のグリコシルトランスフェラーゼ活性を含む原核生物宿主細胞に関する。 本発明はまた、真核生物のグリコシルトランスフェラーゼ活性を含む原核生物宿主細胞を提供する工程およびグリコシル化されたタンパク質を産生するのに有効な条件下で原核生物宿主細胞を培養する工程によるグリコシル化されたタンパク質を産生する方法も開示する。 本開示の別の局面は、細菌の表面に1つまたは複数のグリカンを発現させる工程、細菌の表面または細菌に由来するバクテリオファージの表面の1つまたは複数のグリカン上に標識を付着させる工程、およびハイスループット形式で標識を解析する工程による、細菌またはバクテリオファージをスクリーニングするための方法に関する。 ネイティブな抗原を認識しかつ結合するFv部分と保存アスパラギン残基においてグリコシル化されるFc部分とを含む、グリコシル化された抗体もまた、開示される。 | ||||||
| 9 | 原核生物におけるグリコシル化されたタンパク質の発現方法 | JP2010541578 | 2009-01-05 | JP5667880B2 | 2015-02-12 | マシュー デリーサ; カサンドラ グァリーノ; トマス マンセル; アダム フィッシャー |
| 10 | PSEUDOGENES AND USES THEREOF | EP12859636.8 | 2012-12-19 | EP2795333A1 | 2014-10-29 | CHINNAIYAN, Arul, M.; KUMAR-SINHA, Chandan; KALYANA-SUNDARAM, Shanker |
| The present invention relates to compositions and methods for cancer diagnosis, research and therapy, including but not limited to, cancer markers. In particular, the present invention relates to pseudogenes as diagnostic markers and clinical targets for prostate cancer. | ||||||
| 11 | PSEUDOGENES AND USES THEREOF | EP12859636 | 2012-12-19 | EP2795333A4 | 2016-02-17 | CHINNAIYAN ARUL M; KUMAR-SINHA CHANDAN; KALYANA-SUNDARAM SHANKER |
| 12 | GLYCOSYLATED PROTEIN EXPRESSION IN PROKARYOTES | EP09701421 | 2009-01-05 | EP2240595A4 | 2011-06-29 | DELISA MATTHEW; GUARINO CASSANDRA; MANSELL THOMAS; FISHER ADAM |
| The present invention relates to a prokaryotic host cell comprising eukaryotic glycosyltransferase activity, where the eukaryotic glycosyltransferase activity is eukaryotic dolichyl-linked UDP-GlcNAc transferase activity and eukaryotic mannosyl-transferase activity. Also disclosed is a method of producing a glycosylated protein by providing a prokaryotic host cell comprising the eukaryotic glycosyltransferase activity and culturing the prokaryotic host cell under conditions effective to produce a glycosylated protein. Another aspect of the present invention pertains to a method for screening bacteria or bacteriophages by expressing one or more glycans on the surface of a bacteria, attaching a label on the one or more glycans on the surface of the bacteria or on the surface of a bacteriophage derived from the bacteria, and analyzing the label in a high-throughput format. A glycosylated antibody comprising an Fv portion which recognizes and binds to a native antigen and an Fc portion which is glycosylated at a conserved asparagine residue is also disclosed. | ||||||
| 13 | 원핵생물에서의 글리코실화 단백질 발현 | KR1020107017270 | 2009-01-05 | KR101589554B1 | 2016-02-01 | 델리사매튜; 구아리노카산드라; 만셀토마스; 피셔아담 |
| 본발명은진핵생물글리코실트랜스퍼라제활성을포함하는원핵생물숙주세포로서, 이진핵생물글리코실트랜스퍼라제활성이진핵생물돌리킬연결 UDP-GlcNAc 트랜스퍼라제활성및 진핵생물만노실트랜스퍼라제활성인원핵생물숙주세포에관한것이다. 또한, 본발명은진핵생물글리코실트랜스퍼라제활성을포함하는원핵생물숙주세포를제공하고글리코실화단백질을생산하기에효과적인조건하에원핵생물숙주세포를배양하여글리코실화단백질을생산하는방법을개시한다. 본발명의다른양태는박테리아표면위에하나이상의글리칸을발현시키고, 박테리아표면또는그 박테리아유래의박테리오파지표면위의하나이상의글리칸에표지를부착시키고, 상기표지를고속작업방식으로분석하여박테리아또는박테리오파지를스크리닝하는방법에관한것이다. 또한, 본발명은자연항원을인식하여이에결합하는 Fv 부분, 및보존된아스파라긴잔기에서글리코실화된 Fc 부분을포함하는글리코실화항체를개시한다. | ||||||
| 14 | 원핵생물에서의 글리코실화 단백질 발현 | KR1020107017270 | 2009-01-05 | KR1020100108420A | 2010-10-06 | 델리사매튜; 구아리노카산드라; 만셀토마스; 피셔아담 |
| 본 발명은 진핵생물 글리코실 트랜스퍼라제 활성을 포함하는 원핵생물 숙주 세포로서, 이 진핵생물 글리코실 트랜스퍼라제 활성이 진핵생물 돌리킬 연결 UDP-GlcNAc 트랜스퍼라제 활성 및 진핵생물 만노실 트랜스퍼라제 활성인 원핵생물 숙주 세포에 관한 것이다. 또한, 본 발명은 진핵생물 글리코실 트랜스퍼라제 활성을 포함하는 원핵생물 숙주 세포를 제공하고 글리코실화 단백질을 생산하기에 효과적인 조건하에 원핵생물 숙주 세포를 배양하여 글리코실화 단백질을 생산하는 방법을 개시한다. 본 발명의 다른 양태는 박테리아 표면 위에 하나 이상의 글리칸을 발현시키고, 박테리아 표면 또는 그 박테리아 유래의 박테리오파지 표면 위의 하나 이상의 글리칸에 표지를 부착시키고, 상기 표지를 고속 작업 방식으로 분석하여 박테리아 또는 박테리오파지를 스크리닝하는 방법에 관한 것이다. 또한, 본 발명은 자연 항원을 인식하여 이에 결합하는 Fv 부분, 및 보존된 아스파라긴 잔기에서 글리코실화된 Fc 부분을 포함하는 글리코실화 항체를 개시한다. | ||||||
| 15 | IMMUNOGENIC COMPOSITIONS | EP16177013.6 | 2016-06-29 | EP3263695A1 | 2018-01-03 | The designation of the inventor has not yet been filed |
The present invention relates to the field of native outer membrane vesicles (nOMVs), particularly nOMVs having increased levels of lipoproteins on their surface and use of same in immunogenic compositions. |
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| 16 | GLYCOSYLATED PROTEIN EXPRESSION IN PROKARYOTES | EP09701421.1 | 2009-01-05 | EP2240595B1 | 2015-12-16 | DELISA, Matthew; GUARINO, Cassandra; MANSELL, Thomas; FISHER, Adam |
| The present invention relates to a prokaryotic host cell comprising eukaryotic glycosyltransferase activity, where the eukaryotic glycosyltransferase activity is eukaryotic dolichyl-linked UDP-GlcNAc transferase activity and eukaryotic mannosyl-transferase activity. Also disclosed is a method of producing a glycosylated protein by providing a prokaryotic host cell comprising the eukaryotic glycosyltransferase activity and culturing the prokaryotic host cell under conditions effective to produce a glycosylated protein. Another aspect of the present invention pertains to a method for screening bacteria or bacteriophages by expressing one or more glycans on the surface of a bacteria, attaching a label on the one or more glycans on the surface of the bacteria or on the surface of a bacteriophage derived from the bacteria, and analyzing the label in a high-throughput format. A glycosylated antibody comprising an Fv portion which recognizes and binds to a native antigen and an Fc portion which is glycosylated at a conserved asparagine residue is also disclosed. | ||||||
| 17 | GLYCOSYLATED PROTEIN EXPRESSION IN PROKARYOTES | EP09701421.1 | 2009-01-05 | EP2240595A2 | 2010-10-20 | DELISA, Matthew; GUARINO, Cassandra; MANSELL, Thomas; FISHER, Adam |
| The present invention relates to a prokaryotic host cell comprising eukaryotic glycosyltransferase activity, where the eukaryotic glycosyltransferase activity is eukaryotic dolichyl-linked UDP-GlcNAc transferase activity and eukaryotic mannosyl-transferase activity. Also disclosed is a method of producing a glycosylated protein by providing a prokaryotic host cell comprising the eukaryotic glycosyltransferase activity and culturing the prokaryotic host cell under conditions effective to produce a glycosylated protein. Another aspect of the present invention pertains to a method for screening bacteria or bacteriophages by expressing one or more glycans on the surface of a bacteria, attaching a label on the one or more glycans on the surface of the bacteria or on the surface of a bacteriophage derived from the bacteria, and analyzing the label in a high-throughput format. A glycosylated antibody comprising an Fv portion which recognizes and binds to a native antigen and an Fc portion which is glycosylated at a conserved asparagine residue is also disclosed. | ||||||
| 18 | GLYCOSYLATED PROTEIN EXPRESSION IN PROKARYOTES | PCT/US2009030110 | 2009-01-05 | WO2009089154A3 | 2010-01-14 | DELISA MATTHEW; GUARINO CASSANDRA; MANSELL THOMAS; FISHER ADAM |
| The invention relates to a prokaryotic host cell comprising eukaryotic glycosyttransferase activity, wherein the eukaryotic glycosyltransferase activity is eukaryotic dolichyl-linked UDP-GIcNAc transferase activity and eukaryotic mannosyltransferase activity. Also disclosed is a method of producing a glycosylated protein by providing a prokaryotic host cell comprising the eukaryotic glycosyltransferase activity and culturing the prokaryotic host cell under conditions effective to produce a glycosylated protein. Another aspect of the disclosure pertains to a method for screening bacteria or bacteriophages by expressing one or more glycans on the surface of a bacteria, attaching a label on the one or more glycans on the surface of the bacteria or a bacteriophage derived from the bacteria, and analyzing the label in a high-throughput format. A glycosylated antibody comprising an Fv portion which recognizes and binds to a native antigen and an Fc portion which is glycosylated at a conserved asparagine residue is also disclosed. | ||||||
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