| 序号 | 专利名 | 申请号 | 申请日 | 公开（公告）号 | 公开（公告）日 | 发明人 |
|---|---|---|---|---|---|---|
| 1 | TRANSFORMED CELL WITH ENHANCED SENSITIVITY TO ANTIFUNGAL COMPOUND AND USE THEREOF | US12815843 | 2010-06-15 | US20100285511A1 | 2010-11-11 | Hiroki NAKAJIMA |
| The present invention provides a transformed cell in which a polynucleotide having a nucleotide sequence encoding an amino acid sequence of an osmosensing histidine kinase having no transmembrane region is introduced in a functional form into a cell deficient in at least one hybrid-sensor kinase, a method of assaying the antifungal activity of a test substance using the transformed cell, and a method of searching an antifungal compound using the method, and the like. | ||||||
| 2 | Histidine kinase two-component in Candida albicans | US10116048 | 2002-04-05 | US06824992B2 | 2004-11-30 | Antonio Jose C. Abad; Gil H. Choi; Richard A. Calderone |
| The present invention relates to a histidine kinase, two-component gene (CaHK1) from Candida albicans. CaHK1 encodes a 2471 amino acid protein with an estimated molecular mass of 281.8 kDa. Also provided are vectors, host cells, antibodies and recombinant methods for producing the same. The invention further relates agonists and antagonists and to screening methods for identifying agonists and antagonists of CaHK1 polypeptide activity. The invention additionally relates to diagnostic methods for detecting CaHK1 nucleic acids, polypeptides, and antibodies in a biological sample. The present invention further relates to novel antagonists and vaccines for the prevention or attenuation of infection by Candida albicans. | ||||||
| 3 | Histidine kinase two-component in candida albicans | US10116048 | 2002-04-05 | US20020146738A1 | 2002-10-10 | Antonio Jose C. Abad; Gil H. Choi; Richard A. Calderone |
| The present invention relates to a histidine kinase, two-component gene (CaHK1) from Candida albicans. CaHK1 encodes a 2471 amino acid protein with an estimated molecular mass of 281.8 kDa. Also provided are vectors, host cells, antibodies and recombinant methods for producing the same. The invention further relates agonists and antagonists and to screening methods for identifying agonists and antagonists of CaHK1 polypeptide activity. The invention additionally relates to diagnostic methods for detecting CaHK1 nucleic acids, polypeptides, and antibodies in a biological sample. The present invention further relates to novel antagonists and vaccines for the prevention or attenuation of infection by Candida albicans. | ||||||
| 4 | Signal recognition particle polypeptides and polynucleotides | US09035382 | 1998-03-05 | US06284515B1 | 2001-09-04 | Michael T. Black |
| This invention relates to a novel bacterial ribonucleoprotein complex and the component parts thereof. More specifically, this invention relates to SRP isolated from Staphylococcus aureus and the use of SRP or components thereof in screens for the identification of antimicrobial compounds and to the use of such compounds in therapy. | ||||||
| 5 | Signal recognition particle polypeptide and polynucleotide | JP28996398 | 1998-09-03 | JPH11235183A | 1999-08-31 | BLACK MICHAEL TERENCE |
| PROBLEM TO BE SOLVED: To provide a newly identified polynucleotide useful for e.g. a screening of antimicroorganism compound, diagnoses or treatments by making the identified polynucleotide include a specific polynucleotide sequence. SOLUTION: This compound comprises a polynucleotide sequence selected from the group consisting of (A) a polynucleotide having an identity of >=70% to a polynucleotide encoding a polypeptide comprising an amino acid sequence of formula I or II, (B) a polynucleotide having a sameness of >=70% to a polynucleotide comprising a nucleic acid of e.g. formula III, (C) a polynucleotide encoding a polypeptide comprising an amino acid sequence having an identity of >=70% to a polynucleotide encoding the same maturation polypeptide as that expressed by the Ffh gene or ffs-RNA gene contained in a deposited strain viz. Staphylococcus aureus, amino acid sequence of the formula, (D) a polynucleotide complementary to each component A to C. The polynucleotide is obtained by cultivating a host cell comprising a vector comprising this isolated polynucleotide. | ||||||
| 6 | Histidine kinase | JP18802198 | 1998-05-29 | JPH11113581A | 1999-04-27 | WALLIS NICOLA GAIL; SHILLING LISA CATHERINE; HWANG MING; JAWORSKI DEBORAH D; INGRAHAM KAREN A; GE JAMES YIGONG; HOLMES DAVID JOHN; ZALACAIN MAGDALENA; THROUP JOHN; BISWAS SANJOY; BRYANT ALEXANDER; MARRA ANDREA |
| PROBLEM TO BE SOLVED: To provide a new histidine kinase comprising a separated polypeptide containing an amino acid sequence having a specific ratio of identity over the whole length of a specific amino acid sequence and useful for the screening of antibacterial compounds and the treatment, diagnosis, etc., of infectious diseases. SOLUTION: This new histidine kinase polypeptide is composed of a separated polypeptide having an identity of >=70%, >=80%, >=90% or >=95% to the amino acid sequence of the formula I or the formula II over the whole length of the formula I or the formula II, a separated polypeptide containing the amino acids of the formula I or the formula II, a separated polypeptide consisting of the amino acid sequence of the formula I or the formula II, etc. It is useful e.g. for the screening of antibacterial compounds and the treatment and diagnosis of infection with Streptococcus pneumoniae. The polypeptide can be produced by screening a library originated from chromosome DNA of Streptococcus pneumoniae 0100993 and expressing the obtained gene in a host cell by recombination technique. | ||||||
| 7 | Transformed cell with enhanced sensitivity to antifungal compound and use thereof | EP03256895.8 | 2003-10-30 | EP1415996A2 | 2004-05-06 | Nakajima, Hiroki |
The present invention provides a transformed cell in which a polynucleotide having a nucleotide sequence encoding an amino acid sequence of an osmosensing histidine kinase having no transmembrane region has been introduced in a functional form into a cell deficient in at least one hybrid-sensor kinase, a method of assaying the antifungal activity of a test substance using the transformed cell, and a method of identifying an antifungal compound using the method, and the like. |
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| 8 | Transformed cell with enhanced sensitivity to antifungal compound and use thereof | US12815843 | 2010-06-15 | US07964373B2 | 2011-06-21 | Hiroki Nakajima |
| The present invention provides a transformed cell in which a polynucleotide having a nucleotide sequence encoding an amino acid sequence of an osmosensing histidine kinase having no transmembrane region is introduced in a functional form into a cell deficient in at least one hybrid-sensor kinase, a method of assaying the antifungal activity of a test substance using the transformed cell, and a method of searching an antifungal compound using the method, and the like. | ||||||
| 9 | Transformed cell with enhanced sensitivity to antifungal compound and use thereof | US12794105 | 2010-06-04 | US07888058B2 | 2011-02-15 | Hiroki Nakajima |
| The present invention provides a transformed cell in which a polynucleotide having a nucleotide sequence encoding an amino acid sequence of an osmosensing histidine kinase having no transmembrane region is introduced in a functional form into a cell deficient in at least one hybrid-sensor kinase, a method of assaying the antifungal activity of a test substance using the transformed cell, and a method of searching an antifungal compound using the method, and the like. | ||||||
| 10 | Transformed cell with enhanced sensitivity to antifungal compound and use thereof | US10697036 | 2003-10-31 | US20040137594A1 | 2004-07-15 | Hiroki Nakajima |
| The present invention provides a transformed cell in which a polynucleotide having a nucleotide sequence encoding an amino acid sequence of an osmosensing histidine kinase having no transmembrane region is introduced in a functional form into a cell deficient in at least one hybrid-sensor kinase, a method of assaying the antifungal activity of a test substance using the transformed cell, and a method of searching an antifungal compound using the method, and the like. | ||||||
| 11 | Signal recognition particle polypeptides and polynucleotides | US09943108 | 2001-08-30 | US20030077585A1 | 2003-04-24 | Michael T. Black |
| This invention relates to a novel bacterial ribonucleoprotein complex and the component parts thereof. More specifically, this invention relates to SRP isolated from Staphylococcus aureus and the use of SRP or components thereof in screens for the identification of antimicrobial compounds and to the use of such compounds in therapy. | ||||||
| 12 | Histidine kinase two-component in Candida albicans | US09419291 | 1999-10-15 | US06416989B1 | 2002-07-09 | Antonio Jose C. Abad; Gil H. Choi; Richard A. Calderone |
| The present invention relates to a histidine kinase, two-component gene (CaHK1) from Candida albicans. CaHK1 encodes a 2471 amino acid protein with an estimated molecular mass of 281.8 kDa. Also provided are vectors, host cells, antibodies and recombinant methods for producing the same. The invention further relates agonists and antagonists and to screening methods for identifying agonists and antagonists of CaHK1 polypeptide activity. The invention additionally relates to diagnostic methods for detecting CaHK1 nucleic acids, polypeptides, and antibodies in a biological sample. The present invention further relates to novel antagonists and vaccines for the prevention or attenuation of infection by Candida albicans. | ||||||
| 13 | Sensor histidine kinase of Staphylococcus Aureus | US46086 | 1998-03-23 | US6127147A | 2000-10-03 | Nicola Gail Wallis |
| The invention provides histidine kinase polypeptides and DNA (RNA) encoding histidine kinase polypetides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing histidine kinase polypeptides to screen for antibacterial compounds. | ||||||
| 14 | TRANSFORMED CELL WITH ENHANCED SENSITIVITY TO ANTIFUNGAL COMPOUND AND USE THEREOF | US12794105 | 2010-06-04 | US20100291607A1 | 2010-11-18 | Hiroki NAKAJIMA |
| The present invention provides a transformed cell in which a polynucleotide having a nucleotide sequence encoding an amino acid sequence of an osmosensing histidine kinase having no transmembrane region is introduced in a functional form into a cell deficient in at least one hybrid-sensor kinase, a method of assaying the antifungal activity of a test substance using the transformed cell, and a method of searching an antifungal compound using the method, and the like. | ||||||
| 15 | Transformed cell with enhanced sensitivity to antifungal compound and use thereof | US12272705 | 2008-11-17 | US07759084B2 | 2010-07-20 | Hiroki Nakajima |
| The present invention provides a transformed cell in which a polynucleotide having a nucleotide sequence encoding an amino acid sequence of an osmosensing histidine kinase having no transmembrane region is introduced in a functional form into a cell deficient in at least one hybrid-sensor kinase, a method of assaying the antifungal activity of a test substance using the transformed cell, and a method of searching an antifungal compound using the method, and the like. | ||||||
| 16 | TRANSFORMED CELL WITH ENHANCED SENSITIVITY TO ANTIFUNGAL COMPOUND AND USE THEREOF | US12272705 | 2008-11-17 | US20090104648A1 | 2009-04-23 | Hiroki NAKAJIMA |
| The present invention provides a transformed cell in which a polynucleotide having a nucleotide sequence encoding an amino acid sequence of an osmosensing histidine kinase having no transmembrane region is introduced in a functional form into a cell deficient in at least one hybrid-sensor kinase, a method of assaying the antifungal activity of a test substance using the transformed cell, and a method of searching an antifungal compound using the method, and the like. | ||||||
| 17 | Transformed cell with enhanced sensitivity to antifungal compound and use thereof | US10697036 | 2003-10-31 | US07452688B2 | 2008-11-18 | Hiroki Nakajima |
| The present invention provides a transformed cell in which a polynucleotide having a nucleotide sequence encoding an amino acid sequence of an osmosensing histidine kinase having no transmembrane region is introduced in a functional form into a cell deficient in at least one hybrid-sensor kinase, a method of assaying the antifungal activity of a test substance using the transformed cell, and a method of searching an antifungal compound using the method, and the like. | ||||||
| 18 | Compounds | US09524643 | 2000-03-14 | US06498234B1 | 2002-12-24 | Nicola Gail Wallis |
| The invention provides histidine kinase polypeptides and DNA (RNA) encoding histidine kinase polypetides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing histidine kinase polypeptides to screen for antibacterial compounds. | ||||||
| 19 | Histidine kinase two-component in Candida albicans | US112450 | 1998-07-09 | US6120999A | 2000-09-19 | Antonio Jose C. Abad; Gil H. Choi; Richard A. Calderone |
| The present invention relates to a histidine kinase, two-component gene (CaHK1) from Candida albicans. CaHK1 encodes a 2471 amino acid protein with an estimated molecular mass of 281.8 kDa. Also provided are vectors, host cells, antibodies and recombinant methods for producing the same. The invention further relates agonists and antagonists and to screening methods for identifying agonists and antagonists of CaHK1 polypeptide activity. The invention additionally relates to diagnostic methods for detecting CaHK1 nucleic acids, polypeptides, and antibodies in a biological sample. The present invention further relates to novel antagonists and vaccines for the prevention or attenuation of infection by Candida albicans. | ||||||
| 20 | Histidine kinase | JP23623898 | 1998-07-17 | JPH11178587A | 1999-07-06 | WALLIS NICOLA GAIL; THROUP JOHN |
| PROBLEM TO BE SOLVED: To obtain the subject new enzyme useful for screening of an antibacterial compound or treatment and diagnosis, etc., of infection of a bacterium belonging to genus Streptococcus, comprising a polypeptide containing an amino acid sequence having high identify at various levels to a specific amino acid sequence. SOLUTION: This new isolated histidine kinase polypeptide is composed of an isolated polypeptide containing amino acid sequences having at least 70% identity, 80% identity, 90% identity and 95% identity to an amino acid sequence of formula I or formula II in whole length of the formula I or the formula II, an isolated polypeptide containing an amino acid sequence of the formula I or the formula II and an isolated polypeptide, etc., as an amino acid sequence of the formula I or the formula II and is useful for screening of an antibacterial compound or treatment and diagnosis, etc., of Streptococcus pneumoniae infection. The enzyme is obtained by probing a Streptococcus pneumoniae chromosome DNA library with a labeled oligonucleotide derived from a partial sequence and manifesting the resultant polynucleotide with a manifesting system. | ||||||
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