| 序号 | 专利名 | 申请号 | 申请日 | 公开（公告）号 | 公开（公告）日 | 发明人 |
|---|---|---|---|---|---|---|
| 1 | 生产罗汉果苷的方法和包含其的组合物及其用途 | CN201580061244.3 | 2015-09-10 | CN107109377A | 2017-08-29 | M.伊特金; R.达维多维奇-里卡纳蒂; S.科亨; V.波特诺伊; A.多龙-费根博伊姆; M.佩特雷科夫; S.申; Y.塔德莫; Y.伯格; E.勒温索恩; N.卡特兹; A.A.沙弗; E.奥伦 |
| 提供了可用于罗汉果苷生物合成的，分离的罗汉果苷和罗汉果醇生物合成途径酶多肽。本发明的罗汉果苷生物合成途径酶包括角鲨烯环氧酶(SE)、环氧水合酶(EH)、细胞色素p450(Cyp)、葫芦二烯醇合酶(CDS)和udp‑葡糖基转移酶(UGT)。还提供了使用所述分离的罗汉果苷和罗汉果醇生物合成酶多肽产生罗汉果苷的方法，所述方法包括使罗汉果醇和/或糖基化的罗汉果醇(罗汉果苷)与至少一种本发明的UDP葡萄糖葡糖基转移酶(UGT)酶多肽接触，所述UDP葡萄糖葡糖基转移酶(UGT)酶多肽催化罗汉果醇和/或糖基化的罗汉果醇的葡糖基化，以产生具有额外的葡糖基成分的罗汉果苷，由此产生罗汉果苷。或者或另外提供的是合成罗汉果醇的方法，所述方法包括使罗汉果醇前体底物与一种或多种如本文所述的催化从罗汉果醇前体底物合成罗汉果醇的罗汉果醇生物合成途径酶多肽接触，由此合成罗汉果醇。 | ||||||
| 2 | METHODS OF PRODUCING MOGROSIDES AND COMPOSITIONS COMPRISING SAME AND USES THEREOF | US15510708 | 2015-09-10 | US20170283844A1 | 2017-10-05 | Maxim ITKIN; Rachel DAVIDOVICH-RIKANATI; Shahar COHEN; Vitaly PORTNOY; Adi DORON-FAIGENBOIM; Marina PETREIKOV; Shmuel SHEN; Yaakov TADMOR; Yosef BURGER; Efraim LEWINSOHN; Nurit KATZIR; Arthur A. SCHAFFER; Elad OREN |
| Isolated mogroside and mogrol biosynthetic pathway enzyme polypeptides useful in mogroside biosynthesis are provided. Mogroside biosynthetic pathway enzymes of the invention include squalene epoxidase (SE), expoxy hydratase (EH), cytochrome p450 (Cyp), cucurbitadienol synthase (CDS) and udp-glucosyl-transferase (UGT). Also provided are methods of producing a mogroside using the isolated mogroside and mogrol biosynthetic enzyme polypeptides, the methods comprising contacting a mogrol and/or a glycosylated mogrol (mogroside) with at least one UDP glucose glucosyl transferase (UGT) enzyme polypeptide of the invention catalyzing glucosylation of the mogrol and/or the glucosylated mogrol to produce a mogroside with an additional glucosyl moietie(s), thereby producing the mogroside. Alternatively or additionally provided is a method of synthesizing a mogrol, the method comprising contacting a mogrol precursor substrate with one or more mogrol biosynthetic pathway enzyme polypeptides as described herein catalyzing mogrol synthesis from the mogrol precursor substrate, thereby synthesizing the mogrol. | ||||||
| 3 | METHODS OF PRODUCING MOGROSIDES AND COMPOSITIONS COMPRISING SAME AND USES THEREOF | EP15839739 | 2015-09-10 | EP3191584A4 | 2018-07-18 | ITKIN MAXIM; DAVIDOVICH-RIKANATI RACHEL; COHEN SHAHAR; PORTNOY VITALY; DORON-FAIGENBOIM ADI; PETREIKOV MARINA; SHEN SHMUEL; TADMOR YAAKOV; BURGER YOSEF; LEWINSOHN EFRAIM; KATZIR NURIT; SCHAFFER ARTHUR A; OREN ELAD |
| Isolated mogroside and mogrol biosynthetic pathway enzyme polypeptides useful in mogroside biosynthesis are provided. Mogroside biosynthetic pathway enzymes of the invention include squalene epoxidase (SE), expoxy hydratase (EH), cytochrome p450 (Cyp), cucurbitadienol synthase (CDS) and udp-glucosyl-transferase (UGT). Also provided are methods of producing a mogroside using the isolated mogroside and mogrol biosynthetic enzyme polypeptides, the methods comprising contacting a mogrol and/or a glycosylated mogrol (mogroside) with at least one UDP glucose glucosyl transferase (UGT) enzyme polypeptide of the invention catalyzing glucosylation of the mogrol and/or the glucosylated mogrol to produce a mogroside with an additional glucosyl moietie(s), thereby producing the mogroside. Alternatively or additionally provided is a method of synthesizing a mogrol, the method comprising contacting a mogrol precursor substrate with one or more mogrol biosynthetic pathway enzyme polypeptides as described herein catalyzing mogrol synthesis from the mogrol precursor substrate, thereby synthesizing the mogrol. | ||||||
| 4 | METHODS OF PRODUCING MOGROSIDES AND COMPOSITIONS COMPRISING SAME AND USES THEREOF | EP15839739.8 | 2015-09-10 | EP3191584A1 | 2017-07-19 | ITKIN, Maxim; DAVIDOVICH-RIKANATI, Rachel; COHEN, Shahar; PORTNOY, Vitaly; DORON-FAIGENBOIM, Adi; PETREIKOV, Marina; SHEN, Shmuel; TADMOR, Yaakov; BURGER, Yosef; LEWINSOHN, Efraim; KATZIR, Nurit; SCHAFFER, Arthur A.; OREN, Elad |
| Isolated mogroside and mogrol biosynthetic pathway enzyme polypeptides useful in mogroside biosynthesis are provided. Mogroside biosynthetic pathway enzymes of the invention include squalene epoxidase (SE), expoxy hydratase (EH), cytochrome p450 (Cyp), cucurbitadienol synthase (CDS) and udp-glucosyl-transferase (UGT). Also provided are methods of producing a mogroside using the isolated mogroside and mogrol biosynthetic enzyme polypeptides, the methods comprising contacting a mogrol and/or a glycosylated mogrol (mogroside) with at least one UDP glucose glucosyl transferase (UGT) enzyme polypeptide of the invention catalyzing glucosylation of the mogrol and/or the glucosylated mogrol to produce a mogroside with an additional glucosyl moietie(s), thereby producing the mogroside. Alternatively or additionally provided is a method of synthesizing a mogrol, the method comprising contacting a mogrol precursor substrate with one or more mogrol biosynthetic pathway enzyme polypeptides as described herein catalyzing mogrol synthesis from the mogrol precursor substrate, thereby synthesizing the mogrol. | ||||||
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