| 序号 | 专利名 | 申请号 | 申请日 | 公开（公告）号 | 公开（公告）日 | 发明人 |
|---|---|---|---|---|---|---|
| 1 | 用于固氮的转基因植物 | CN201480062171.5 | 2014-09-15 | CN105874070A | 2016-08-17 | 芭芭拉·莱因霍尔德-胡雷克; 托马斯·胡雷克; 胡利伟; 王祺; 杨海元 |
| 提供了可用于增加植物的产量、生物量、生长速率、活力、氮利用效率和/或非生物胁迫耐受性，优选地对养分缺乏的耐受性的基因材料和核酸序列。具体地，描述了栽培植物中固氮特性的改善。 | ||||||
| 2 | 用于刺激植物生长、保护植物以及将杆菌孢子固定在植物上的融合蛋白和方法 | CN201480015271.2 | 2014-03-17 | CN105263965A | 2016-01-20 | B·汤普森; K·汤普森 |
| 本发明总体上涉及含有靶向序列的融合蛋白，所述靶向序列使融合蛋白靶向蜡样芽孢杆菌家族成员的外孢壁。本发明还涉及表达所述融合蛋白的重组蜡样芽孢杆菌家族成员以及含有表达所述融合蛋白的所述重组蜡样芽孢杆菌家族成员的制剂。还描述了通过向植物或植物生长介质施用所述重组蜡样芽孢杆菌家族成员或所述制剂刺激植物生长、保护植物免于病原体的侵害以及提高植物的胁迫抗性的方法。本发明还涉及用于将表达融合蛋白的重组蜡样芽孢杆菌家族成员的孢子固定在植物上的方法。 | ||||||
| 3 | 植物の生育を刺激し、植物を保護し、および、植物上にバチルスの芽胞を固定するための融合タンパク質および方法 | JP2016502564 | 2014-03-17 | JP2016520525A | 2016-07-14 | ブライアン・トンプソン; ケイティ・トンプソン |
| 本発明は概して、融合タンパク質をBacillus cereusファミリーのメンバーのエキソスポリウムに向かわせる標的化配列を含有する融合タンパク質を目的とするものである。本発明はまた、そのような融合タンパク質を発現する組換えBacillus cereusファミリーのメンバー、および、当該融合タンパク質を発現する組換えBacillus cereusファミリーのメンバーを含有する製剤に関するものである。植物または植物生育環境に、当該組換えBacillus cereusファミリーのメンバー、または当該製剤を適用することによる、植物の生育を刺激する方法、病原体から植物を防御する方法、および、植物におけるストレス耐性を増強させる方法もまた記述される。本発明はまた、融合タンパク質を発現する組換えBacillus cereusファミリーのメンバーの芽胞を植物に固定する方法に関する。 | ||||||
| 4 | JPH07501201A - | JP50555292 | 1992-03-02 | JPH07501201A | 1995-02-09 | |
| 5 | Synthetic gene clusters | US15288916 | 2016-10-07 | US09957509B2 | 2018-05-01 | Ethan Mirsky; Karsten Temme; Christopher A. Voigt; Dehua Zhao |
| Methods for making synthetic gene clusters are described. | ||||||
| 6 | SYNTHETIC GENE CLUSTERS | US15706353 | 2017-09-15 | US20180073028A1 | 2018-03-15 | Ethan Mirsky; Karsten Temme; Christopher A. Voigt; Dehua Zhao |
| Methods for making synthetic gene clusters are described. | ||||||
| 7 | Fusion proteins and methods for stimulating plant growth, protecting plants, and immobilizing bacillus spores on plants | US14775892 | 2014-03-17 | US09850289B2 | 2017-12-26 | Brian Thompson; Katie Thompson |
| The present invention is generally directed to fusion proteins containing a targeting sequence that targets the fusion protein to the exosporium of a Bacillus cereus family member. The invention also relates to recombinant Bacillus cereus family members expressing such fusion proteins and formulations containing the recombinant Bacillus cereus family members expressing the fusion proteins. Methods for stimulating plant growth, for protecting plants from pathogens, and for enhancing stress resistance in a plant by applying the recombinant Bacillus cereus family members or the formulations to plants or a plant growth medium are also described. The invention also relates to methods for immobilizing spores of a recombinant Bacillus cereus family member expressing a fusion protein on plants. | ||||||
| 8 | PLANTS CAPABLE OF NITROGEN FIXATION | US15309713 | 2015-05-04 | US20170335294A1 | 2017-11-23 | Alexander KRICHEVSKY |
| Present invention discloses plants and plant cells comprising Streptomyces thermoautotrophicus nitrogenase and capable able of nitrogen fixation. Methods to generate said plants and plant cells are disclosed. This invention is instrumental for producing plants, including agriculturally important crops, with reduced or abolished requirements for nitrogen fertilizer. | ||||||
| 9 | Methods and System for Photo-activated Hydrogen Generation | US14620386 | 2015-02-12 | US20150225742A1 | 2015-08-13 | Deborah B. Maxwell |
| Systems and methods for providing alternative fuel, in particular hydrogen photocatalytically generated by a system comprising photoactive nanoparticles and a nitrogenase cofactor are provided. In one aspect, the system includes a water soluble cadmium selenide nanoparticle (CdSe) surface capped with mercaptosuccinate (CdSe-MSA); and a NafY.FeMo-co complex comprising a NafY protein and an iron-molybdenum cofactor (FeMo-co); wherein the CdSe-MSA and the NafY.FeMo-co complex are present in about 1:1 molar ratio in a CdSe-MSA.NafY.FeMo-co system. In various embodiments, when illuminated, the CdSe-MSA.NafY.FeMo-co system is capable of photocatalytically producing hydrogen gas for an extended period of, e.g., at least 5 hours, at least 10 hours, or at least 90 hours. Methods for making and using the same are also provided. | ||||||
| 10 | PROCESS FOR ISOLATION BY CRYSTALLIZATION OF THE Mo-Fe PROTEIN OF THE ENZYME NITROGENASE | US3668074D | 1969-12-17 | US3668074A | 1972-06-06 | BURNS RICHARD CHARLES |
| Disclosed is a process for obtaining crystals of the Mo-Fe protein fraction of the enzyme nitrogenase. This fraction is essential in the fixation of atmospheric nitrogen by certain microorganisms.
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| 11 | FUSION PROTEINS AND METHODS FOR STIMULATING PLANT GROWTH, PROTECTING PLANTS, AND IMMOBILIZING BACILLUS SPORES ON PLANTS | US15846487 | 2017-12-19 | US20180208630A1 | 2018-07-26 | Brian Thompson; Katie Thompson |
| The present invention is generally directed to fusion proteins containing a targeting sequence that targets the fusion protein to the exosporium of a Bacillus cereus family member. The invention also relates to recombinant Bacillus cereus family members expressing such fusion proteins and formulations containing the recombinant Bacillus cereus family members expressing the fusion proteins. Methods for stimulating plant growth, for protecting plants from pathogens, and for enhancing stress resistance in a plant by applying the recombinant Bacillus cereus family members or the formulations to plants or a plant growth medium are also described. The invention also relates to methods for immobilizing spores of a recombinant Bacillus cereus family member expressing a fusion protein on plants. | ||||||
| 12 | NANOPARTICLE BIOHYBRID COMPLEXES | US15818450 | 2017-11-20 | US20180142264A1 | 2018-05-24 | Paul Wayne KING; Katherine Alice BROWN; Lance C. SEEFELDT; John W. PETERS; Gordana DUKOVIC |
| Disclosed herein are biohybrid protein complexes capable of using light energy to photocatalyze the reduction of N2 into NH3. Also provided are methods of using biohybrid protein complexes to enzymatically reduce N2 to NH3 using light rather than chemical energy as the driving force. These methods may also include the production and isolation of ammonia, hydrogen or both. | ||||||
| 13 | SYNTHETIC GENE CLUSTERS | US15288916 | 2016-10-07 | US20170152519A1 | 2017-06-01 | Ethan Mirsky; Karsten Temme; Christopher A. Voigt; Dehua Zhao |
| Methods for making synthetic gene clusters are described. | ||||||
| 14 | Methods and system for photo-activated hydrogen generation | US14620386 | 2015-02-12 | US09605279B2 | 2017-03-28 | Deborah B. Maxwell |
| Systems and methods for providing alternative fuel, in particular hydrogen photocatalytically generated by a system comprising photoactive nanoparticles and a nitrogenase cofactor are provided. In one aspect, the system includes a water soluble cadmium selenide nanoparticle (CdSe) surface capped with mercaptosuccinate (CdSe-MSA); and a NafY.FeMo-co complex comprising a NafY protein and an iron-molybdenum cofactor (FeMo-co); wherein the CdSe-MSA and the NafY.FeMo-co complex are present in about 1:1 molar ratio in a CdSe-MSA.NafY.FeMo-co system. In various embodiments, when illuminated, the CdSe-MSA.NafY.FeMo-co system is capable of photocatalytically producing hydrogen gas for an extended period of, e.g., at least 5 hours, at least 10 hours, or at least 90 hours. Methods for making and using the same are also provided. | ||||||
| 15 | nifH promoters of Bradyrhizobium | US109869 | 1987-10-16 | US5008194A | 1991-04-16 | Barry G. Rolfe; John Shine; Kieran F. Scott; Hauke Hennecke |
| The nifH promoter regions of Bradyrhizobium japonicum and Bradyrhizobium sp. (parasponia) have been sequenced and found to be significantly homologous. Recombinant DNA molecules comprising foreign genes under the control of such promoters are provided. Rhizobial species containing such recombinant constructions, either in plasmids or integrated into the genome, are provided. These are especially useful for expressing desired foreign genes within root nodules. | ||||||
| 16 | nifD promoter of Bradyrhizobium | US109868 | 1987-10-16 | US5001061A | 1991-03-19 | Barry G. Rolfe; John Shine; Kieran F. Scott; Hauke Hennecke |
| The nifD promoter regions of Bradyrhizobium japonicum and Bradyhizobium sp. (Parasponia) have been sequenced and found to be significantly homologous. Recombinant DNA molecules comprising foreign genes under the control of such promoters are provided. Rhizobial species containing such recombinant constructions, either in plasmids or integrated into the genome, are provided. These are especially useful for expressing desired foreign genes within root nodules. | ||||||
| 17 | Genes involved with hydrogenase and nitrogenase activities in rhizobium japonicum and cosmids | EP86107490.4 | 1986-06-03 | EP0205071A2 | 1986-12-17 | Maier, Robert J. |
Replicons are produced by combining a cloning vector and insert hup/nif DNA isolated from slow-growing species of Rhizobium, such as Rhizobium japonicum, and used to insert such hup/nif DNA into Hup and/or Nif species of Rhizobium. |
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| 18 | Recombinant DNA molecules | EP84304191.4 | 1984-06-21 | EP0130047A1 | 1985-01-02 | Shine, John; Rolfe, Barry G.; Scott, Kieran F. |
There is provided a bacterial strain containing and replicating therein a recombinant DNA plasmid comprising:
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| 19 | FUSION PROTEINS AND METHODS FOR STIMULATING PLANT GROWTH, PROTECTING PLANTS, AND IMMOBILIZING BACILLUS SPORES ON PLANTS | EP14763028 | 2014-03-17 | EP2970507A4 | 2016-12-14 | THOMPSON BRIAN; THOMPSON KATIE |
| 20 | SYNTHETIC GENE CLUSTERS | EP12800054.4 | 2012-06-14 | EP2721153A2 | 2014-04-23 | MIRSKY, Ethan; TEMME, Karsten; VOIGT, Chris; ZHAO, Dehua |
| Methods for making synthetic gene clusters are described. | ||||||
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