| 序号 | 专利名 | 申请号 | 申请日 | 公开（公告）号 | 公开（公告）日 | 发明人 |
|---|---|---|---|---|---|---|
| 1 | 包含磷酸酮醇酶的重组宿主细胞 | CN201180039718.6 | 2011-06-16 | CN103140584A | 2013-06-05 | M.道纳; L.A.马吉奥-霍尔; J-F.汤布 |
| 本发明涉及重组宿主细胞，其包含：（i）编码多肽的内源性基因中的至少一个缺失、突变或替换，所述多肽将丙酮酸转化为乙醛、乙酰-磷酸或乙酰-CoA；和（ii）编码具有磷酸酮醇酶活性的多肽的异源性多核苷酸。本发明也涉及重组宿主细胞，其还包含（iii）编码具有磷酸转乙酰酶活性的多肽的异源性多核苷酸。 | ||||||
| 2 | 类固醇多步氧化方法和所用的遗传工程细胞 | CN89108378.2 | 1989-09-23 | CN1042567A | 1990-05-30 | 哈门·斯利克会斯; 格拉杜斯·考那利斯·玛丽·塞尔顿; 埃里克·巴斯廷·斯迈尔 |
| 本发明提供了一种基团工程构建的宿主细胞，该细胞能够同时氧化类固醇，优选同时导入17α-羟基和C21-羟基基团。特别是氧化是用细胞进行的，在该细胞中插入有编码至少两种涉及胆固醇向氢化可的松生化转化的蛋白质。合适的宿主细胞包括杆菌属、酵母属或Kluyreromyces菌珠。该新的宿主细胞适于胆固醇、孕甾烯醇酮、孕甾酮和17α-羟基-孕甾酮的生化氧化，它们都是所说的生化转化的中间产物。该宿主细胞还可用于最终制备多基因系统，该系统能一步将胆固醇转化为氢化可的松。 | ||||||
| 3 | 类固醇生物化学氧化的方法和用于此方法中的遗传工程方法产生的细胞 | CN89104208.3 | 1989-05-06 | CN1038667A | 1990-01-10 | 荷曼·斯利杰克惠斯; 格拉杜斯·考尼利斯·玛利亚·塞尔坦; 埃利克·巴斯提安·思马尔 |
| 本发明提供了含有新的表达基因盒的遗传工程方法得到的宿主细胞，它能完成类固醇的生物化学氧化作用，尤其是用导入了编码了与胆甾醇转化为氢化可的松的生物途径有关的蛋白质的DNA的细胞来完成氧化作用。合适的宿主细胞有杆菌属(Bacillus)、酵母菌属(Saccharomgces)或Kluyveromyces的菌种。这些新的宿主细胞适合于胆甾醇，导甾烯醇酮、孕酮、17α-孕酮和11-脱氧皮质醇的微生物氧化作用，它们是所述生物途径的中间产物。这些新的表达基因盒在最终产生一个一步完成胆甾醇向氢化可的松转化的多基因系统中也是有用的。 | ||||||
| 4 | Yeast strain which autonomously produce the steroid | JP2002561663 | 2002-01-29 | JP4620932B2 | 2011-01-26 | エリク・デグリーズ; ジール・カウエ; ジャック・ウィンテ; ティルマン・アハシュテッター; デニ・ポンポン; ブルーノ・デュマ; ロベルト・スパニョーリ |
| 5 | Biochemical oxidation of steroid | JP7855699 | 1999-03-23 | JPH11308991A | 1999-11-09 | SLIJKHUIS HERMAN; SELTEN GERARDUS CORNELIS MARIA; SMAAL ERIC BASTIAAN |
| PROBLEM TO BE SOLVED: To economically obtain a steroid useful as a medicine or the like by incubating a sterol compound in the presence of protein(s) produced by a specific process under such conditions as to enable a steroid to be oxidized and the resulting oxidation product to be accumulated in the culture fluid. SOLUTION: This steroid is obtained by incubating a sterol compound in the presence of protein(s) under such conditions as to enable a steroid to be oxidized and the resulting oxidation product to be accumulated in the culture fluid, followed by recovering the oxidation product. The above reaction is an in vitro selective biochemical oxidation, and the above-mentioned protein(s) is produced by the following procedure: a bovine adrenocortical cDNA library is screened with a probe consisting of a partial sequence of bovine cytochrome P 450 steroid 21-hydroxylase (P 450C21) gene, the P 450C21 gene thus obtained is integrated into a vector and expressed in host cells. By using the above protein(s) in the aforementioned process, hydrocortisone or the like useful as a medicine can be economically obtained by several stage oxidation of a sterol compound. COPYRIGHT: (C)1999,JPO | ||||||
| 6 | RECOMBINANT HOST CELLS COMPRISING PHOSPHOKETOLASES | EP11727094.2 | 2011-06-16 | EP2593552B1 | 2017-02-15 | DAUNER, Michael; MAGGIO-HALL, Lori, Ann; TOMB, Jean-Francois |
| The present invention is related to recombinant host cells comprising: (i) at least one deletion, mutation, and/or substitution in an endogenous gene encoding a polypeptide that converts pyruvate to acetaldehyde, acetyl-phosphate or acetyl-CoA; and (ii) a heterologous polynucleotide encoding a polypeptide having phosphoketolase activity. The present invention is also related to recombinant host cells further comprising (iii) a heterologous polynucleotide encoding a polypeptide having phosphotransacetylase activity. | ||||||
| 7 | Process for the biochemical oxidation of steroids and genetically engineered cells to be used therefor | EP89201173.5 | 1989-05-08 | EP0340878B1 | 2001-05-16 | Slijkhuis, Harmen; Selten, Gerardus Cornelis Maria; Smaal, Erik Bastiaan |
| 8 | The novel aromatic ring dioxygenase gene group and its applications | JP2004570723 | 2003-11-26 | JP4390713B2 | 2009-12-24 | 典彦 三沢; 一敏 新藤; 由紀 笠井 |
| 9 | Biochemical oxidation methods and genetically engineered cells used for the steroid | JP50570789 | 1989-05-08 | JP2963711B2 | 1999-10-18 | SUREIKUHAISU HERUMAN; SERUTEN HERARUDEYUSU KORUNERISU MARIA; SUMAARU ERITSUKU BASUCHIAAN |
| 10 | JPH04500303A - | JP50570789 | 1989-05-08 | JPH04500303A | 1992-01-23 | |
| 11 | NEW POLYPEPTIDE HAVING FERREDOXIN-NADP+ REDUCTASE ACTIVITY, POLYNUCLEOTIDE ENCODING THE SAME AND USES THEREOF | US15748020 | 2016-07-22 | US20180216138A1 | 2018-08-02 | Philippe Soucaille; Isabelle Meynial-Salles; Céline Foulquier; Antoine Riviere |
| The present invention relates to a novel polypeptide having the enzymatic activity of reduction of NADP+ using electrons from reduced ferredoxin (Ferredoxin-NADP+ reductase activity), a polynucleotide having a nucleotide sequence encoding such polypeptide and uses thereof. The invention relates to the modulation of the Ferredoxin NADP+ reductase activity in a microorganism by varying the expression level of the polynucleotide coding for such polypeptide. The invention also relates to the production of commodity chemicals, especially ethanol, n-butanol, 1,3-propanediol, 1,2-propanediol, isopropanol and acetone by fermenting microorganisms wherein their Ferredoxin NADP+ reductase activity is modulated. | ||||||
| 12 | Recombinant Host Cells Comprising Phosphoketalase | US15375858 | 2016-12-12 | US20170088863A1 | 2017-03-30 | Michael Dauner; Lori Ann Maggio-Hall; Jean-Francois Tomb |
| The present invention is related to recombinant host cells comprising: (i) at least one deletion, mutation, and/or substitution in an endogenous gene encoding a polypeptide that converts pyruvate to acetaldehyde, acetyl-phosphate or acetyl-CoA; and (ii) a heterologous polynucleotide encoding a polypeptide having phosphoketolase activity. The present invention is also related to recombinant host cells further comprising (iii) a heterologous polynucleotide encoding a polypeptide having phosphotransacetylase activity. | ||||||
| 13 | Recombinant host cells comprising phosphoketolases | US14335734 | 2014-07-18 | US09550999B2 | 2017-01-24 | Michael Dauner; Lori Ann Maggio-Hall; Jean-Francois Tomb |
| The present invention is related to recombinant host cells comprising: (i) at least one deletion, mutation, and/or substitution in an endogenous gene encoding a polypeptide that converts pyruvate to acetaldehyde, acetyl-phosphate, or acetyl-CoA; and (ii) a heterologous polynucleotide encoding a polypeptide having phosphoketolase activity. The present invention is also related to recombinant host cells further comprising (iii) a heterologous polynucleotide encoding a polypeptide having phosphotransacetylase activity. | ||||||
| 14 | Nonribosomal peptide synthetases | US14713662 | 2015-05-15 | US09487763B2 | 2016-11-08 | David H. Sherman; Michael Marie Kaufman-Schofield; Sunit Jain; Gregory Dick |
| The present disclosure is directed to the biosynthetic pathway for a nonribosomal peptide synthetase (NRPS)-derived drug and analogs thereof. The invention provides polynucleotide sequences useful for heterologous expression in a convenient microbial host for the synthesis of the NRPS-derived drug, the polypeptides encoded by such polynucleotides, expression vectors comprising the polynucleotides, host cells comprising the polynucleotides or expression vectors, and kits comprising a host cell. Also provided is a method for the production of ET-743, the NRPS-derived drug. | ||||||
| 15 | Hydrogenase fusion protein for improved hydrogen production | US13791550 | 2013-03-08 | US09133486B2 | 2015-09-15 | Phillip Richard Smith; James R. Swartz |
| Compositions of a fusion protein comprising a spatially tethered ferredoxin-NADP-reductase (FNR) and an active [FeFe] hydrogenase, genetic sequences encoding such fusion proteins, and methods of use thereof are provided. The fusion proteins of the invention link an FNR polypeptide to an active [FeFe] hydrogenase through a polypeptide linker. The fusion protein facilitates improved electron transfer through a ferredoxin, and allows direct electron transfer from NADPH to the hydrogenase. | ||||||
| 16 | RECOMBINANT HOST CELLS COMPRISING PHOSPHOKETOLASES | US14335734 | 2014-07-18 | US20140349349A1 | 2014-11-27 | MICHAEL DAUNER; Lori Ann Maggio-Hall; Jean-Francois Tomb |
| The present invention is related to recombinant host cells comprising: (i) at least one deletion, mutation, and/or substitution in an endogenous gene encoding a polypeptide that converts pyruvate to acetaldehyde, acetyl-phosphate, or acetyl-CoA; and (ii) a heterologous polynucleotide encoding a polypeptide having phosphoketolase activity. The present invention is also related to recombinant host cells further comprising (iii) a heterologous polynucleotide encoding a polypeptide having phosphotransacetylase activity. | ||||||
| 17 | GENE THAT IMPARTS OXYGEN RESISTANCE AND APPLICATION THEREOF | US12995296 | 2009-06-12 | US20120021418A1 | 2012-01-26 | Masaki Serata; Tomoyuki Sako |
| To provide a gene useful for imparting oxygen resistance to a microorganism and use of the gene.The oxygen-resistance-imparting gene encoding a protein selected from among the following proteins (a) to (c): (a) a protein having the amino acid sequence of SEQ ID NO: 2 or 6; (b) a protein which has an amino acid sequence equivalent to the amino acid sequence of (a), except that one to several amino acid residues are deleted, substituted, or added, and which exhibits oxygen-resistance-imparting activity; and (c) a protein which has an amino acid sequence having an identity of 85% or higher to the amino acid sequence of (a), and which exhibits oxygen-resistance-imparting activity. | ||||||
| 18 | Yeast strains autonomously producing steroids | US10470673 | 2002-01-29 | US07670829B2 | 2010-03-02 | Roberto Spagnoli; Tilman Achstetter; Gilles Cauet; Eric Degryse; Bruno Dumas; Denis Pompon; Jacques Winter |
| The present invention relates to genetically modified yeast strains autonomously producing, from a simple carbon source, steroids. The invention also relates to a method for producing steroids from such yeast strains. | ||||||
| 19 | Process for oxidation of steroids and genetically engineered cells used therein | US10462128 | 2003-06-16 | US20040067579A1 | 2004-04-08 | Herman Slijkhuis; Eric Bastiaan Smaal; Gerardus Cornelis Maria Selten |
| An expression cassette, operable in a recombinant host, comprising a heterologous DNA coding sequence encoding a protein, which is functional, alone or in cooperation with one or more additional proteins, of catalyzing an oxidation step in the biological pathway for conversion of cholesterol into hydrocortisone, which step is selected from the group consisting of: the conversion of cholesterol to pregnenolone; the conversion of pregnenolone to progesterone; the conversion of progesterone to 17null-hydroxy-progesterone; the conversion of 17null-hydroxyprogesterone to cortexolone; the conversion of cortexolone to hydrocortisone, and the corresponding control sequences effective in said host. | ||||||
| 20 | ENZYMES AND METHODS FOR DEALKYLATION OF SUBSTRATES | US15065717 | 2016-03-09 | US20160265006A1 | 2016-09-15 | Christopher W. JOHNSON; Gregg T. BECKHAM |
| Disclosed herein are enzymes and organisms useful for the dealkylation of products derived from lignin depolymerization, including the conversion of guaiacol or guaethol to catechol or the conversion of anisole to phenol. Methods of converting guaiacol or guaethol to catechol or anisole to phenol using enzymes or organisms expressing the same are also disclosed. | ||||||
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