| 序号 | 专利名 | 申请号 | 申请日 | 公开（公告）号 | 公开（公告）日 | 发明人 |
|---|---|---|---|---|---|---|
| 1 | 细胞法产生葡糖二酸 | CN200980120747.8 | 2009-04-03 | CN102066552A | 2011-05-18 | 文泰石; 尹相活; 克丽斯塔拉·拉内特·琼斯·普拉瑟 |
| 本发明涉及通过重组表达肌醇-1-磷酸合酶、肌醇加氧酶和糖醛酸脱氢酶在细胞内产生葡糖醛酸和葡糖二酸。本发明还公开了对编码糖醛酸脱氢酶之基因的克隆和表征。 | ||||||
| 2 | 一种重组大肠杆菌及其构建方法与通过代谢工程生产葡萄糖二酸的方法 | CN201710200371.8 | 2017-03-30 | CN106929459A | 2017-07-07 | 娄文勇; 苏慧慧; 宗敏华; 徐培; 杨继国 |
| 本发明公开了一种重组大肠杆菌及其构建方法与通过代谢工程生产葡萄糖二酸的方法，属于代谢工程领域。本发明通过在大肠杆菌中表达来源于植物的肌醇‑1‑磷酸合成酶基因（Ino1）、肌醇氧化酶基因（MIOX），实现葡萄糖醛酸的生产途径，同时表达来源于根癌农杆菌（Agrobacterium tumefaciens）的醛酸脱氢酶基因（Udh），成功构建葡萄糖二酸的合成途径。其中，重组大肠杆菌在LB‑G（LB培养基中加入10g/L葡萄糖）发酵培养基中生成葡萄糖二酸2.53g/L。本发明提出的重组大肠杆菌发酵生产葡萄糖二酸的方法，具有广阔的发展前景，为生物合成葡萄糖二酸奠定基础。 | ||||||
| 3 | 一种构建重组酵母发酵生产葡萄糖二酸的方法 | CN201410568277.4 | 2014-10-22 | CN104312935A | 2015-01-28 | 康振; 陈坚; 堵国成; 王淼; 刘叶 |
| 本发明公开了一种构建重组酵母发酵生产葡萄糖二酸的方法，属于代谢工程领域。本发明通过在酵母中表达肌醇-1-磷酸合成酶基因(Ino1)、肌醇加氧酶基因(MIOX)，实现葡萄糖醛酸的生成途径，同时表达来源于恶臭假单胞菌(Pseudomonas putida)的醛酸脱氢酶(Udh)，成功构建了葡萄糖二酸的合成途径。其中，重组毕赤酵母在YPD发酵培养基中生成葡萄糖二酸40mg/L，在YPD-MI(YPD培养基中加入60mM肌醇)发酵培养基中生成葡萄糖二酸60mg/L。本发明提出的重组酵母发酵生成葡萄糖二酸的方法，具有广阔的发展前景，为生物合成葡萄糖二酸奠定了基础。 | ||||||
| 4 | 一种构建重组酵母生物合成葡萄糖醛酸的方法 | CN201410568261.3 | 2014-10-22 | CN104312934A | 2015-01-28 | 康振; 陈坚; 堵国成; 王淼; 刘叶 |
| 本发明公开了一种构建重组酵母生物合成葡萄糖醛酸的方法，属于代谢工程领域。本发明将不同来源的肌醇加氧酶基因(MIOX)在不同酵母宿主中表达，以葡萄糖或肌醇为底物，实现了葡萄糖醛酸的生物合成。其中，重组毕赤酵母菌株在YPD发酵培养基中生成葡萄糖醛酸20mg/L,在YPD-MI(YPD培养基中加入60mM肌醇)发酵培养基中生成葡萄糖醛酸50mg/L。本发明提出的重组酵母合成葡萄糖醛酸的方法，具有广阔的发展前景，为生物法合成葡萄醛酸提供了新的思路。 | ||||||
| 5 | 细胞法产生葡糖二酸 | CN200980120747.8 | 2009-04-03 | CN102066552B | 2014-03-12 | 文泰石; 尹相活; 克丽斯塔拉·拉内特·琼斯·普拉瑟 |
| 本发明涉及通过重组表达肌醇-1-磷酸合酶、肌醇加氧酶和糖醛酸脱氢酶在细胞内产生葡糖醛酸和葡糖二酸。本发明还公开了对编码糖醛酸脱氢酶之基因的克隆和表征。 | ||||||
| 6 | CELLULAR PRODUCTION OF GLUCARIC ACID | US12935983 | 2009-04-03 | US20110124065A1 | 2011-05-26 | Tae Seok Moon; Sang-Hwal Yoon; Kristala Lanett Jones Prather |
| The invention relates to the production of glucuronic and glucaric acid in cells through recombinant expression of myo-inositol 1-phosphate synthase, myo-inositol oxygenase and uronate dehydrogenase. Cloning and characterization of the gene encoding uronate dehydrogenase is also disclosed. | ||||||
| 7 | CELLULAR PRODUCTION OF GLUCARIC ACID | US14452066 | 2014-08-05 | US20150093794A1 | 2015-04-02 | TAE SEOK MOON; Sang-Hwal Yoon; Kristala Lanett Jones Prather |
| The invention relates to the production of glucuronic and glucaric acid in cells through recombinant expression of myo-inositol 1-phosphate synthase, myo-inositol oxygenase and uronate dehydrogenase. Cloning and characterization of the gene encoding uronate dehydrogenase is also disclosed. | ||||||
| 8 | Cellular production of glucaric acid through recombinant expression of uronate dehydrogenase and myo-inositol oxygenase | US12935983 | 2009-04-03 | US08835147B2 | 2014-09-16 | Tae Seok Moon; Sang-Hwal Yoon; Kristala Lanett Jones Prather |
| The invention relates to the production of glucuronic and glucaric acid in cells through recombinant expression of myo-inositol 1-phosphate synthase, myo-inositol oxygenase and uronate dehydrogenase. Cloning and characterization of the gene encoding uronate dehydrogenase is also disclosed. | ||||||
| 9 | Production in cells of the glucaric acid | JP2011502991 | 2009-04-03 | JP2011516063A | 2011-05-26 | ジョーンズ プレイサー，クリスタラ，ラネット; ムン，テ，ソク; ユン，サン−ファル |
| 本発明は、ミオイノシトール１−リン酸シンターゼ、ミオイノシトールオキシゲナーゼ、およびウロン酸デヒドロゲナーゼの組換え発現を介した、細胞内でのグルクロン酸およびグルカン酸の産生に関する。 ウロン酸デヒドロゲナーゼをコードする遺伝子のクローニングおよび特徴づけも開示される。 | ||||||
| 10 | CELLULAR PRODUCTION OF GLUCARIC ACID | EP09755201.2 | 2009-04-03 | EP2274417A2 | 2011-01-19 | MOON, Tae, Seok; YOON, Sang-Hwal; PRATHER, Kristala, Lanett Jones |
| The invention relates to the production of glucuronic and glucaric acid in cells through recombinant expression of myo-inositol 1 -phosphate synthase, myo-inositol oxygenase and uronate dehydrogenase. Cloning and characterization of the gene encoding uronate dehydrogenase is also disclosed. | ||||||
| 11 | グルカン酸の細胞での産生 | JP2011502991 | 2009-04-03 | JP6196016B2 | 2017-09-13 | ムン,テ,ソク; ユン,サン−ファル; プレイサー,クリスタラ,ラネット ジョーンズ |
| 12 | グルカン酸の細胞での産生 | JP2016190435 | 2016-09-28 | JP2017018140A | 2017-01-26 | ムン,テ,ソク; ユン,サン−ファル; プレイサー,クリスタラ,ラネット ジョーンズ |
| 【課題】本発明は、ウロン酸デヒドロゲナーゼをコードする遺伝子を組換え技術により発現し、かつミオイノシトールオキシゲナーゼをコードする遺伝子を組換え技術により発現する細胞を提供する。本発明はまた、グルクロン酸およびグルカン酸を産生する方法であって、本発明に関連する細胞を培養してグルクロン酸またはグルカン酸を産生すること、およびグルクロン酸またはグルカン酸を細胞から回収することを含む、前記方法も提供する。 【解決手段】本発明は、ミオイノシトール1−リン酸シンターゼ、ミオイノシトールオキシゲナーゼ、およびウロン酸デヒドロゲナーゼの組換え発現を介した、細胞内でのグルクロン酸およびグルカン酸の産生に関する。ウロン酸デヒドロゲナーゼをコードする遺伝子のクローニングおよび特徴づけも開示される。 【選択図】なし |
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| 13 | CELLULAR PRODUCTION OF GLUCARIC ACID | EP09755201.2 | 2009-04-03 | EP2274417B1 | 2013-07-24 | MOON, Tae, Seok; YOON, Sang-Hwal; PRATHER, Kristala, Lanett Jones |
| 14 | CELLULAR PRODUCTION OF GLUCARIC ACID | EP09755201 | 2009-04-03 | EP2274417A4 | 2012-05-02 | MOON TAE SEOK; YOON SANG-HWAL; PRATHER KRISTALA LANETT JONES |
| 15 | 글루카르산의 세포적 제조 | KR1020107024705 | 2009-04-03 | KR101625443B1 | 2016-05-30 | 문,태,석; 윤,상-활; 프래더,크리스탈라,라넷존스 |
| 본발명은미오-이노시톨 1-포스페이트신타제, 미오-이노시톨옥시게나제및 우로네이트데히드로게나제의재조합발현을통해세포에서글루쿠론산및 글루카르산을제조하는것에관한것이다. 또한, 우로네이트데히드로게나제를코딩하는유전자의클로닝및 특성분석이개시되어있다. | ||||||
| 16 | 글루카르산의 세포적 제조 | KR1020107024705 | 2009-04-03 | KR1020110027652A | 2011-03-16 | 문,태,석; 윤,상-활; 프래더,크리스탈라,라넷존스 |
| 본 발명은 미오-이노시톨 1-포스페이트 신타제, 미오-이노시톨 옥시게나제 및 우로네이트 데히드로게나제의 재조합 발현을 통해 세포에서 글루쿠론산 및 글루카르산을 제조하는 것에 관한 것이다. 또한, 우로네이트 데히드로게나제를 코딩하는 유전자의 클로닝 및 특성분석이 개시되어 있다.
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| 17 | CELLULAR PRODUCTION OF GLUCARIC ACID | PCT/US2009002111 | 2009-04-03 | WO2009145838A2 | 2009-12-03 | MOON TAE SEOK; YOON SANG-HWAL; PRATHER KRISTALA LANETT JONES |
| The invention relates to the production of glucuronic and glucaric acid in cells through recombinant expression of myo-inositol 1 -phosphate synthase, myo-inositol oxygenase and uronate dehydrogenase. Cloning and characterization of the gene encoding uronate dehydrogenase is also disclosed. | ||||||
| 18 | CRYSTAL STRUCTURE OF A SUBSTRATE COMPLEX OF MIOX | PCT/NZ2007000154 | 2007-06-14 | WO2007145539A2 | 2007-12-21 | LOOMES KERRY L; BROWN PETER M; CARADOC-DAVIES TOM T; DICKSON JAMES; COOPER GARTH J S; BAKER EDWARD N |
| Diabetes mellitus is a chronic disease that is associated with altered metabolism of the inositol sugars myo-inositol (MI) and D-chiro-inositol (DCI). In animals, catabolism of MI and DCI depends on the enzyme myo-inositol oxygenase (MIOX), which catalyzes the first committed step of the glucuronate-xylulose pathway, and is found almost exclusively in the kidneys. The crystal structure of MIOX, in complex with MI, has been determined by multiwavelength anomalous diffraction methods and refined at 2.0-A resolution (R = 0.208, Rfree = 0.258). The structure reveals a monomeric, single-domain protein with a mostly-helical fold that is distantly related to the diverse HD domain superfamily. Five helices form the structural core and provide six ligands (4 His and 2 Asp) for the di-iron center, in which the two iron atoms are bridged by a putative hydroxide ion and one of the aspartate ligands, Asp124. A key loop forms a lid over the MI substrate, which is coordinated in bidentate mode to one iron atom. It is proposed that this mode of iron coordination, and interaction with a key lysine residue, activate MI for bond cleavage. The structure also reveals the basis of substrate specificity and suggests routes for the development of specific MIOX inhibitors for treatment of inositol-associated disorders. | ||||||
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