序号 专利名 申请号 申请日 公开(公告)号 公开(公告)日 发明人
1 OXYGEN TOLERANT HYDROGENASE BY MUTATING ELECTRON SUPPLY PATHWAY US15255833 2016-09-02 US20170067034A1 2017-03-09 James Robert Swartz; Jamin Koo
Compositions and methods are provided for an O2 tolerant Fe—Fe hydrogenase. The hydrogenases of the invention comprise specific amino acid substitutions relative to the native, or wild-type enzymes.
2 재조합 미생물을 이용한 수소 생산 방법 KR1020090058043 2009-06-29 KR1020110000776A 2011-01-06 차형준; 김영환
PURPOSE: A method for producing hydrogen using microorganism which is transformed with (nickel-iron)-hydrogenase gene is provided to enhance efficiency of biological hydrogenation and to apply as an electrocatalyst. CONSTITUTION: A (nickel-iron)-hydrogenase protein which is derived from Hydrogenovibrio marinus has an amino acid sequence of sequence number 2. A gene encoding the (nickel-iron)-hydrogenase protein has a base sequence of sequence number 1. A recombinant vector has the gene. A method for producing hydrogen from microorganism comprises: a step of transforming the microorganism with the recombinant vector; and a step of culturing the transformed microorganism in a culture medium containing Ni and Fe under the microaerobic condition. The microorganism is E.coli.
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