| 序号 | 专利名 | 申请号 | 申请日 | 公开（公告）号 | 公开（公告）日 | 发明人 |
|---|---|---|---|---|---|---|
| 1 | 一种杀虫晶体蛋白的非受体结合蛋白组合物及其提取方法和应用 | CN201610103013.0 | 2016-02-25 | CN105669842A | 2016-06-15 | 林毅; 刘肖萍; 古宇清; 余小强 |
| 本发明公开了一种杀虫晶体蛋白的非受体结合蛋白组合物及其提取方法和应用，该非受体结合蛋白组合物包括但不局限于微管蛋白、谷胱甘肽合成酶、ATP合成酶、精氨酸激酶、凝集素、胰蛋白酶、翻译延伸因子和细胞色素C氧化酶，且该非受体结合蛋白组合物是通过亲和层析自包含中肠肠腔液的害虫中肠的水溶性物质中提取的，上述亲和层析所用的亲和分子为杀虫晶体蛋白Cry。应用本发明的非受体结合蛋白组合物可在不改变工厂现有产品基础上，提高Cry的杀虫活性，克服害虫对现有产品所产生的抗性问题，以及一些重要害虫对现有产品活性不高的问题。 | ||||||
| 2 | 一种宽壳全海笋线粒体COI基因扩增引物 | CN201610021309.8 | 2016-01-14 | CN105462995A | 2016-04-06 | 张婷婷; 李莉; 王英俊; 邹琰; 刘童; 刘洪军; 王慧 |
| 本发明提供了一种宽壳全海笋线粒体COI基因，其核苷酸序列为SEQ ID NO:1；上述的基因序列用于设计检测宽壳全海笋的扩增引物。本发明的引物是针对宽壳全海笋线粒体COI基因片段设计的，相对软体动物通用的COI基因引物，具有绝对的特异性优势。该引物的扩增效率高达90％以上，扩增效率高，大大降低了扩增的时间和费用。 | ||||||
| 3 | 一种具有生物活性的COX |
CN201710194719.7 | 2017-03-29 | CN106929488A | 2017-07-07 | 李臣鸿; 白天宇; 王梅杰 |
| 本发明提供了一种具有生物活性的COX52‑69多肽的固相合成方法，该多肽的氨基酸序列如SEQ ID NO.1所示，所述固相合成方法包括以下步骤：按照多肽的顺序重复添加肽链上的下一氨基酸，直至整条肽链18个氨基酸全部添加完成并检测通过；加入切割液，在通风橱中室温搅拌裂解，制得多肽粗品；利用高效液相色谱检测合成的多肽粗品，并分析合成产物中多肽的纯度；用质谱检测多肽粗品的分子量，确定合成的多肽是否是目标产物。本发明利用化学合成的多肽在动物体内体外实验，均发现化学合成的多肽有抑制葡萄糖引起的胰岛素分泌的功能和减轻体重的作用，能够用于制备抵抗高胰岛素血症和减轻体重的药物中，其具有生物活性。 | ||||||
| 4 | 一种多肽片段以及制备方法和用途 | CN201310471571.9 | 2013-10-11 | CN103571803A | 2014-02-12 | 李臣鸿; 陈正望 |
| 本发明提供了一种多肽片段，经检测后发现其为细胞色素C氧化酶Ⅷ(cytochromeCoxidasesubunitⅧ)的一个片段，由细胞色素C氧化酶Ⅷ的52-69位的氨基酸残基组成，我们记作COX52-69。其氨基酸序列为：LLPAGWVLSH LDSYKKRE。经实验证明该多肽片段具有抑制胰岛分泌胰岛素的作用。 | ||||||
| 5 | Especially for improving the appearance of the skin and / or hair, a combination of light and cytochrome c oxidase substrate | JP2010545537 | 2009-02-06 | JP2011511049A | 2011-04-07 | カン・ラン・ンギュイヤン; ジュリアン・ラブロー; ダン−マン・ファム |
| The invention relates to the combination of a light ray with a cytochrome C oxydase substrate particularly for improving the appearance of the skin and/or the hair. The invention more particularly relates to a method for a cosmetic treatment particularly for improving the appearance of the skin and/or the hair that comprises the simultaneous and/or sequential administration of a) at least one cytochrome C oxydase substrate and/or at least one agent increasing the expression of said substrate; and b) at least one light radiation having at least one predominant wavelength that activates the cytochrome C oxydase. More particularly, said light radiation has at least one predominant wavelength activating the cytochrome C oxydase, that ranges from 550 to 1000 nm, in particular from 550 to 800 nm, preferably from 620 to 700 nm, and more preferably from 640 to 680 nm; the method comprises applying a dose preferably ranging from 0.01 to 200 J/cm2, preferably from 0.1 to 30 J/cm2, more preferably from 1 to 30 J/cm2, or even from 5 to 30 J/cm2. The invention also relates to a composition containing at least one cytochrome C oxydase substrate and/or one agent increasing the expression of said substrate, and at least one compound emitting and/or filtering, in particular under light exposure, a light radiation having at least one predominant wavelength that activates the cytochrome C oxydase. The invention also relates to a kit including at least one composition containing at least one cytochrome C oxydase substrate and/or one agent increasing the expression of said substrate, and at least one device and/or compound emitting and/or filtering a light radiation having at least one predominant wavelength that activates the cytochrome C oxydase. | ||||||
| 6 | 不斉酸化反応を有する蛋白質複合体およびその製造方法 | JP2014545783 | 2013-11-08 | JPWO2014073673A1 | 2016-09-08 | 宏行 永岡 |
| 補酵素の添加なしに2級アルコールの一方の鏡像体を選択的に不斉酸化し、且つ、酸素存在下水溶媒系にて不斉酸化活性を有する蛋白質複合体とその製造方法、及び該蛋白質複合体の高分子化合物コーティングに関わる製造方法を提供する。動植物由来水溶性粗蛋白質をゲルに包括する処理、該ゲルを空気酸化する処理、該ゲルから蛋白質複合体を水溶液中に溶出させる処理を有する第1の工程と、前記蛋白質複合体水溶液に重力を掛けて蛋白質複合体を濃縮沈殿させる処理、該蛋白質複合体沈殿物を0.5mM程度のグリシン水酸化ナトリウム水溶液にて再溶解し高分子化合物と均一に共存させる処理、該共存水溶液を再沈殿させて脱水乾燥させて高分子化合物コーティング蛋白質複合体を得る第2の工程と、を含むことを特徴とする不斉酸化反応を有する蛋白質複合体の製造方法。 | ||||||
| 7 | 特に皮膚および/または髪の外観を改善するための、光線とチトクロームCオキシダーゼ基質との組合せ | JP2010545537 | 2009-02-06 | JP5791902B2 | 2015-10-07 | ジュリアン・ラブロー; カン・ラン・ンギュイヤン; ダン−マン・ファム |
| 8 | MODIFIED CYTOCHROME c OXIDASE AND MITOCHONDRIA | JP2002231042 | 2002-08-08 | JP2004065147A | 2004-03-04 | YOSHIKAWA SHINYA; SHIMADA HIDEO; SHIMOKATA KUNITOSHI |
| <P>PROBLEM TO BE SOLVED: To modify an active hydrogen ion transfer activity of a cytochrome c oxidase of mitochondria. <P>SOLUTION: An aspartic acid residue at the 51st position from the N-terminus of a subunit I of the cytochrome c oxidase by utilizing a method (patent application number 2002-202279) for transferring a protein to the mitochondria, applied by the inventor, etc. The cytochrome c oxidase having the modified subunit I contains the modified subunit I in which the aspartic acid residue at the 51st position from the N-terminus of the amino acid sequence of the subunit I of the cytochrome c oxidase of a vertebrate or at the corresponding position is modified. <P>COPYRIGHT: (C)2004,JPO | ||||||
| 9 | UVに関連するmtDNAの融合転写物ならびにその方法および使用 | JP2012555266 | 2011-03-01 | JP6008743B2 | 2016-10-19 | ハーボトル,アンドリュー; ダクボ,ガブリエル; パー,ライアン エル.; クリード,ジェニファー; レギュリ,ブライアン; ロビンソン,ケリー |
| 10 | Fusion transcripts as well as the method and its use of mtDNA associated with UV | JP2012555266 | 2011-03-01 | JP2013520965A | 2013-06-10 | ハーボトル，アンドリュー; ダクボ，ガブリエル; エル． パー，ライアン; クリード，ジェニファー; レギュリ，ブライアン; ロビンソン，ケリー |
| 本発明は、ＵＶ曝露に関連する新規のミトコンドリア融合転写物および関連する欠失分子を提供する。 ｍｔＤＮＡ分子および関連する融合転写物をインビボおよびインビトロにて検出するための方法、ならびに、スキンケア製品のスクリーニングおよび試験におけるこれらの使用もまた提供される。 | ||||||
| 11 | Transformed plant, and a manufacturing method thereof comprising a transgene comprising a hybrid nucleic acid sequence comprising at least one mitochondrial gene fragment uncalibrated from higher plants | JP51774494 | 1994-02-15 | JPH08506245A | 1996-07-09 | アルジャンドロ アラヤ; アーマン ムーラ |
| (57)【要約】 高次の植物の未校正ミトコンドリア遺伝子のコード化領域を少なくとも含み、前記配列を含む植物の雄性稔性を制御するハイブリッド核酸配列、そのような配列を有する形質転換植物および形質転換雄性不稔植物の製造方法および雄性稔性植物の復原方法。 形質転換植物の核は、発現され得るハイブリッド配列（トランス遺伝子）を含み、高次の植物の未校正ミトコンドリア遺伝子のコード化領域及び前記コード化領域により発現されたタンパク質をミトコンドリアに移入することのできる配列の少なくとも１種を含み、前記ハイブリッド配列は前記トランス遺伝子が組み込まれた植物の雄性稔性を修飾することができ、一方前記植物の他の表現型の特徴は無変化のままである。 | ||||||
| 12 | Protein complex capable of catalyzing asymmetric oxidation reaction and method for producing same | US14441657 | 2013-11-08 | US09982242B2 | 2018-05-29 | Hiroyuki Nagaoka |
| Provided are: a protein complex capable of selectively and asymmetrically oxidizing an enantiomer of a secondary alcohol without adding a coenzyme and having an asymmetric oxidation activity in a water-soluble solvent system in the presence of oxygen; a method for producing the same; and a method for coating the protein complex with a high molecular weight compound. The method for producing the protein complex includes: (1) enclosing a crude water-soluble protein in a gel, air-oxidizing the gel, and eluting the protein complex into an aqueous solution; and (2) applying gravity to concentrate and precipitate the protein complex, redissolving the precipitate in an aqueous glycine sodium hydroxide solution of about 0.5 mM and allowing the same to homogeneously coexist with a high molecular weight compound, and re-precipitating the solution and dehydrating and drying the same to yield a protein complex coated with a high molecular weight compound. | ||||||
| 13 | Combination of a light ray with a cytochrome C oxidase substrate particularly for improving the appearance of the skin and/or hair | US14802616 | 2015-07-17 | US09827311B2 | 2017-11-28 | Julien Laboureau; Quang Lan Nguyen; Dang-Man Pham |
| The present invention relates more specifically to a cosmetic treatment method intended in particular to improve the appearance of the skin and/or hair comprising the simultaneous and/or sequential administration: a) of at least one cytochrome C oxidase substrate and/or of at least one agent which increases the expression of the said substrate; and b) of at least one light radiation exhibiting at least one predominant wavelength which activates cytochrome C oxidase. In particular, the said light radiation exhibits at least one predominant wavelength which activates cytochrome C oxidase ranging from 550 to 1000 nm, in particular from 550 to 800 nm, preferably from 620 to 700 nm and more preferably still from 640 to 680 nm and is preferably used at a dose ranging from 0.01 to 200 J/cm2, preferably from 0.1 to 30 J/cm2, more preferably from 1 to 30 J/cm2, indeed even from 5 to 30 J/cm2.The invention also relates to a composition comprising at least one cytochrome C oxidase substrate and/or one agent which increases the expression of the said substrate and at least one compound emitting and/or filtering, in particular under exposure to light, a light radiation exhibiting at least one predominant wavelength which activates cytochrome C oxidase, and to a kit comprising at least one composition comprising at least one cytochrome C oxidase substrate and/or one agent which increases the expression of the said substrate and one device and/or one compound emitting/filtering a light radiation exhibiting at least one predominant wavelength which activates cytochrome C oxidase. | ||||||
| 14 | UV Associated mtDNA Fusion Transcripts and Methods and Uses Thereof | US15347601 | 2016-11-09 | US20170175194A1 | 2017-06-22 | Andrew Harbottle; Gabriel Dakubo; Ryan L. Parr; Jennifer Creed; Brian Reguly; Kerry Robinson |
| The present invention provides novel mitochondrial fusion transcripts and related deletion molecules that are associated with UV exposure. Methods for in vivo and in vitro detection of mtDNA molecules and associated fusion transcripts is also provided, as is their use in the screening and testing of skin care products. | ||||||
| 15 | UV Associated mtDNA Fusion Transcripts and Methods and Uses Thereof | US14569147 | 2014-12-12 | US20150322516A1 | 2015-11-12 | Andrew Harbottle; Gabriel Dakubo; Ryan L. Parr; Jennifer Creed; Brian Reguly; Kerry Robinson |
| The present invention provides novel mitochondrial fusion transcripts and related deletion molecules that are associated with UV exposure. Methods for in vivo and in vitro detection of mtDNA molecules and associated fusion transcripts is also provided, as is their use in the screening and testing of skin care products. | ||||||
| 16 | IMPORTATION OF MITOCHODRIAL PROTEIN BY AN ENHANCED ALLOTOPIC APPROACH | US14323240 | 2014-07-03 | US20140377869A1 | 2014-12-25 | Marisol CORRAL-DEBRINSKI; Jose-Alain SAHEL; Valerie KALTIMBACHER; Crystel BONNET |
| An expression vector containing appropriate mitochondrion-targeting sequences (MTS) and appropriate 3′UTR sequences provides efficient and stable delivery of a mRNA encoding a protein (CDS) to the mitochondrion of a mammalian cell. The MTS and 3′UTR sequences guide the CDS mRNA from the nuclear compartment of the cell to mitochondrion-bound polysomes, where the CDS is translated. This provides an efficient translocation of a mature functional protein into the mitochondria. A method of targeting mRNA expressed in the nuclear compartment of a mammalian cell to the mitochondrion is also provided. The vector and methods can be used to treat defects in mitochondrial function. | ||||||
| 17 | UV ASSOCIATED MTDNA FUSION TRANSCRIPTS AND METHODS AND USES THEREOF | US13582049 | 2011-03-01 | US20140024025A1 | 2014-01-23 | Andrew Harbottle; Gabriel Dakubo; Ryan L. Parr; Jennifer Creed; Brian Reguly; Kerry Robinson |
| The present invention provides novel mitochondrial fusion transcripts and related deletion molecules that are associated with UV exposure. Methods for in vivo and in vitro detection of mtDNA molecules and associated fusion transcripts is also provided, as is their use in the screening and testing of skin care products. | ||||||
| 18 | Transgenic plants including a transgene consisting of a hybrid nucleic acid sequence, comprising at least one unedited mitochondrial gene fragment from higher plants and process for producing them | US505218 | 1995-11-03 | US5914447A | 1999-06-22 | Alejandro Araya; Armand Mouras |
| Hybrid nucleic acid sequences including at least the coding region of an unedited mitochondrial gene of superior plants and controlling the male fertility of plants containing said sequences, transgenic plants having such sequences and methods of production of transgenic male-sterile plants and method of restoring male-fertile plants. The nuclei of the transgenic plants contain a hybrid sequence capable of being expressed (transgene), comprising at least one coding region of an unedited mitochondrial gene of superior plants and a sequence capable of transferring the protein expressed by said coding region, to the mitochondrion, said hybrid sequence being capable of modifying the male fertility of plants having incorporated said transgene, while leaving the other phenotype characteristics of said plants unaltered. | ||||||
| 19 | THERAPEUTIC COMPOSITIONS INCLUDING FRATAXIN, LACTOFERRIN, AND MITOCHONDRIAL ENERGY GENERATING ENZYMES, AND USES THEREOF | EP15799189.4 | 2015-05-27 | EP3148565A2 | 2017-04-05 | WILSON, D. Travis |
| Disclosed herein are methods and compositions for the treatment and/or prevention of diseases or conditions comprising administration of a therapeutic biological molecule, and/or naturally or artificially occurring derivatives, analogues, or pharmaceutically acceptable salts thereof, alone or in combination with one or more active agents (e.g., an aromatic-cationic peptide). The present technology provides compositions related to aromatic-cationic peptides linked to a therapeutic biological molecule and uses of the same. In some embodiments, the aromatic-cationic peptide comprises 2′,6′-dimethyl-Tyr-D-Arg-Phe-Lys-NH2, Phe-D-Arg-Phe-Lys-NH2, or D-Arg-2′,6′-Dmt-Lys-Phe-NH2. | ||||||
| 20 | PROTEIN COMPLEX CAPABLE OF CATALYZING ASYMMETRIC OXIDATION REACTION AND METHOD FOR PRODUCING SAME | EP13853909.3 | 2013-11-08 | EP2918683A1 | 2015-09-16 | NAGAOKA, Hiroyuki |
Provided are: a protein complex capable of selectively and asymmetrically oxidizing one of enantiomers of a secondary alcohol without adding a coenzyme and having an asymmetric oxidation activity in a water-soluble solvent system in the presence of oxygen; a method for producing the same; and a production method relating to coating of the protein complex with a high molecular compound. A method for producing a protein complex capable of catalyzing an asymmetric oxidation reaction, said method being characterized by comprising: a first step that comprises a treatment for enclosing a crude water-soluble protein derived from an animal or plant in a gel, a treatment for air-oxidizing the gel, and a treatment for eluting the protein complex from the gel into an aqueous solution; and a second step that comprises a treatment for applying gravity to the aqueous solution of the protein complex to concentrate and precipitate the protein complex, a treatment for redissolving the protein complex precipitate in an aqueous glycine sodium hydroxide solution of about 0.5 mM and allowing the same to homogeneously coexist with a high molecular compound, and a treatment for re-precipitating the coexistence solution and dehydrating and drying the same to give the protein complex coated with the high molecular compound. |
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