| 序号 | 专利名 | 申请号 | 申请日 | 公开（公告）号 | 公开（公告）日 | 发明人 |
|---|---|---|---|---|---|---|
| 1 | 通过下调LDH和PDHK表达降低乳酸水平和增加多肽生产 | CN201180026517.2 | 2011-05-26 | CN102985437B | 2016-05-25 | M·周; B·R·斯奈德克尔; C·K·D·恩格; A·沈 |
| 本发明提供了用于降低培养的细胞中乳酸生产和增加多肽生产的方法和组合物。在一个方面，本发明提供了这样的方法，所述方法包括培养表达a)乳酸脱氢酶(LDH)特异性的小干扰RNA(siRNA)和b)丙酮酸脱氢酶激酶(PDHK)特异性的siRNA的细胞。在另一个方面，本发明提供了培养包含LDH特异性的siRNA和PDHK特异性的siRNA的细胞或载体。 | ||||||
| 2 | 通过下调LDH和PDHK表达降低乳酸水平和增加多肽生产 | CN201180026517.2 | 2011-05-26 | CN102985437A | 2013-03-20 | M·周; B·R·斯奈德克尔; C·K·D·恩格; A·沈 |
| 本发明提供了用于降低培养的细胞中乳酸生产和增加多肽生产的方法和组合物。在一个方面，本发明提供了这样的方法，所述方法包括培养表达a)乳酸脱氢酶(LDH)特异性的小干扰RNA(siRNA)和b)丙酮酸脱氢酶激酶(PDHK)特异性的siRNA的细胞。在另一个方面，本发明提供了培养包含LDH特异性的siRNA和PDHK特异性的siRNA的细胞或载体。 | ||||||
| 3 | 用于增强甲羟戊酸、异戊二烯和类异戊二烯的产量的重组微生物 | CN201280031900.1 | 2012-04-27 | CN103930541A | 2014-07-16 | Z·Q·贝克; M·C·米勒; C·M·派瑞斯; Y·A·普里马克; J·P·普奇; D·H·韦尔斯 |
| 本发明描述了通过为了增加流向甲羟戊酸生产的碳通量在如下酶途径中对微生物进行工程改造使得所述酶活性中的一者或多者受到调节来增加甲羟戊酸、戊二烯、类异戊二烯前体分子和/或类异戊二烯的产量的组合物和方法：(a)柠檬酸合酶、(b)磷酸转乙酰酶、(c)乙酸激酶、(d)乳酸脱氢酶、(e)苹果酸酶和(f)丙酮酸脱氢酶。此外，可通过异源表达mvaE和mvaS基因(例如但不限于来自生物体格氏李斯特菌DSM20601、屎肠球菌、鹑鸡肠球菌EG2和铅黄肠球菌的mvaE和mvaS基因)进一步增大甲羟戊酸、戊二烯、类异戊二烯前体分子和/或类异戊二烯的产量。 | ||||||
| 4 | 用于制备3-羟基丙酸和其它产物的方法 | CN201080053692.6 | 2010-09-27 | CN102695799A | 2012-09-26 | M·D·林奇; R·T·吉尔; T·瓦内克-利普斯科布 |
| 本发明涉及经代谢工程改造的微生物菌株，如细菌菌株，其中对用于化学产物制备的丙二酰-CoA具有提高的利用率，所述化学产物包括3-羟基丙酸。 | ||||||
| 5 | LDH及びPDHK発現を下方制御することによる乳酸レベルの低下及びポリペプチド生産の増加 | JP2018076234 | 2018-04-11 | JP2018138030A | 2018-09-06 | チョウ, メイシア; スネデカー, ブラッドリー リチャード; ング, チー ギン ドミンゴス; シェン, エイミー |
| 【課題】培養細胞における乳酸生産を低下させ、ポリペプチド生産を増加させる方法及び組成物の提供。 【解決手段】培養細胞における乳酸生産を低下させる方法であって、乳酸脱水素酵素(LDH)に特異的な低分子干渉RNA(siRNA)をコードする第一異種性核酸配列、及びピルビン酸脱水素酵素キナーゼ(PDHK)に特異的なsiRNAをコードする第二異種性核酸配列を含んでなる細胞を培養することを含んでなり、第一異種性核酸配列は第一プロモーターに作動的に連結され、第二異種性核酸配列は第二プロモーターに作動的に連結されている方法。 【選択図】なし |
||||||
| 6 | ピルビン酸デヒドロゲナーゼ変異体、それを含む微生物及びそれを用いるＬ−アミノ酸生産方法 | JP2017548259 | 2016-03-15 | JP2018512132A | 2018-05-17 | ホ、ラン; ムン、ジュン オク; ベ、ヒュン ウォン; キム、ヒョン ジュン; ソン、スン ギ |
| 本発明は、新規なピルビン酸デヒドロゲナーゼ(pyruvate dehydrogenase)変異体、前記変異体をコードするポリヌクレオチド、前記ピルビン酸デヒドロゲナーゼ変異体を含む、L−アミノ酸の生産能を有するコリネバクテリウム属微生物及び前記微生物を利用してL−アミノ酸を生産する方法に関する。【選択図】なし | ||||||
| 7 | ELECTROCHEMICAL BIOREACTOR MODULE AND ENGINEERED METABOLIC PATHWAYS FOR 1- BUTANOL PRODUCTION WITH HIGH CARBON EFFICIENCY | EP16756175 | 2016-02-23 | EP3262179A4 | 2018-07-11 | DODDS DAVID R; ARMIGER WILLIAM B; KOFFAS MATTHEOS |
| A combination of an electrochemical device for delivering reducing equivalents to a cell, and engineered metabolic pathways within the cell capable of utilizing the electrochemically provided reducing equivalents is disclosed. Such a combination allows the production of commodity chemicals by fermentation to proceed with increased carbon efficiency. | ||||||
| 8 | METHOD FOR PRODUCING 3-HYDROXYPROPIONIC ACID AND OTHER PRODUCTS | EP10819620.5 | 2010-09-27 | EP2480673B1 | 2018-05-23 | LYNCH, Michael, D.; GILL, Ryan, T.; WARNECKE-LIPSCOMB, Tanya |
| This invention relates to metabolically engineered microorganism strains, such as bacterial strains, in which there is an increased utilization of malonyl-CoA for production of a chemical product, which includes 3-hydroxypropionic acid. | ||||||
| 9 | PYRUVATE DEHYDROGENASE MUTANT, MICROORGANISM COMPRISING MUTANT, AND METHOD FOR PRODUCING L-AMINO ACID BY USING MICROORGANISM | EP16765246.0 | 2016-03-15 | EP3272860A1 | 2018-01-24 | HUH, Lan; MOON, Jun Ok; BAE, Hyun Won; KIM, Hyung Joon; SONG, Sung Ki |
The present disclosure relates to a novel pyruvate dehydrogenase variant, a polynucleotide encoding the pyruvate dehydrogenase variant, a microorganism of the genus Corynebacterium producing L-amino acid, which includes the pyruvate dehydrogenase variant, and a method for producing an L-amino acid using the microorganism. |
||||||
| 10 | ENGINEERING OF ACETYL-CoA METABOLISM IN YEAST | EP14854424 | 2014-10-17 | EP3058059A4 | 2017-09-27 | NIELSEN JENS; SIEWERS VERENA; KRIVORUCHKO ANASTASIA; ZHANG YIMING; ZHOU YONGJIN; BUIJS NICOLAAS A A; DAI ZONGJIE |
| 11 | BIOCONVERSION OF SHORT-CHAIN HYDROCARBONS TO FUELS AND CHEMICALS | US15562606 | 2016-03-31 | US20180355394A1 | 2018-12-13 | Ramon Gonzales |
| An engineered microorganism(s) with novel pathways for the conversion of short-chain hydrocarbons to fuels and chemicals (e.g. carboxylic acids, alcohols, hydrocarbons, and their alpha-, beta-, and omega-functionalized derivatives) is described. Key to this approach is the use of hydrocarbon activation enzymes able to overcome the high stability and low reactivity of hydrocarbon compounds through the cleavage of an inert C—H bond. Oxygen-dependent or oxygen-independent activation enzymes can be exploited for this purpose, which when combined with appropriate pathways for the conversion of activated hydrocarbons to key metabolic intermediates, enables the generation of product precursors that can subsequently be converted to desired compounds through established pathways. These novel engineered microorganism(s) provide a route for the production of fuels and chemicals from short chain hydrocarbons such as methane, ethane, propane, butane, and pentane. | ||||||
| 12 | METHOD FOR PRODUCING 3-HYDROXYPROPIONIC ACID AND OTHER PRODUCTS | US15167439 | 2016-05-27 | US20170114377A1 | 2017-04-27 | Michael D. Lynch; Ryan T. Gill; Tanya E. W. Lipscomb |
| This invention relates to metabolically engineered microorganism strains, such as bacterial strains, in which there is an increased utilization of malonyl-CoA for production of a chemical product, which includes 3-hydroxypropionic acid. | ||||||
| 13 | Method for producing 3-hydroxypropionic acid and other products | US14575927 | 2014-12-18 | US09428778B2 | 2016-08-30 | Michael D. Lynch; Ryan T. Gill; Tanya E.W. Lipscomb |
| This invention relates to metabolically engineered microorganism strains, such as bacterial strains, in which there is an increased utilization of malonyl-CoA for production of a chemical product, which includes 3-hydroxypropionic acid. | ||||||
| 14 | Methods for producing 3-hydroxypropionic acid and other products | US13916534 | 2013-06-12 | US09388419B2 | 2016-07-12 | Michael D. Lynch; Ryan T. Gill; Tanya E. W. Lipscomb |
| This invention relates to metabolically engineered microorganism strains, such as bacterial strains, in which there is an increased utilization of malonyl-CoA for production of a chemical product, which includes 3-hydroxypropionic acid. | ||||||
| 15 | ALDEHYDE DEHYDROGENASE MUTANT, POLYNUCLEOTIDE ENCODING THE MUTANT, VECTOR AND MICROORGANISM HAVING THE POLYNUCLEOTIDE, AND METHOD OF PRODUCING 1,4-BUTANEDIOL BY USING THE SAME | US14596000 | 2015-01-13 | US20150284694A1 | 2015-10-08 | Yukyung Jung; Jinhwan Park; Jieun Kim; Hwayoung Cho; Kwangmyung Cho |
| An aldehyde dehydrogenase mutant polypeptide, a recombinant microorganism including a polynucleotide encoding the polypeptide, and a method of producing 1,4-butanediol by using the same. | ||||||
| 16 | Recombinant microorganisms for enhanced production of mevalonate, isoprene, and isoprenoids | US13459067 | 2012-04-27 | US09121038B2 | 2015-09-01 | Zachary Q. Beck; Michael C. Miller; Caroline M. Peres; Yuliya A. Primak; Jeff P. Pucci; Derek H. Wells |
| The invention features compositions and methods for the increased production of mevalonate, isoprene, isoprenoid precursor molecules, and/or isoprenoids in microorganisms by engineering a microorganism for increased carbon flux towards mevalonate production in the following enzymatic pathways: (a) citrate synthase, (b) phosphotransacetylase, (c) acetate kinase, (d) lactate dehydrogenase, (e) malic enzyme, and (f) pyruvate dehydrogenase such that one of more of the enzyme activity is modulated. In addition, production of mevalonate, isoprene, isoprenoid precursor molecules, and/or isoprenoids can be further enhanced by the heterologous expression of the mvaE and mvaS genes (such as, but not limited to, mvaE and mvaS genes from the organisms Listeria grayi DSM 20601, Enterococcus faecium, Enterococcus gallinarum EG2, and Enterococcus casseliflavus). | ||||||
| 17 | METHODS AND COMPOSITIONS FOR THE AUGMENTATION OF PYRUVATE AND ACETYL-COA FORMATION | US14417540 | 2013-07-26 | US20150203824A1 | 2015-07-23 | Frank A. Skraly |
| The present disclosure identifies methods and compositions for modifying photoautotrophic organisms as hosts, such that the organisms efficiently convert carbon dioxide and light into pyruvate or acetyl-CoA, and in particular the use of such organisms for the commercial production of molecules derived from these precursors, e.g., ethanol. | ||||||
| 18 | METHOD OF PRODUCING L-AMINO ACIDS | US14498124 | 2014-09-26 | US20150104836A1 | 2015-04-16 | Jun Ok MOON; Sang Jo LIM; Do Hyun KWON; Kwang Ho LEE; Hyun Won BAE |
| Provided is a method of producing L-amino acids by using a recombinant coryneform microorganism in which the expression of a target gene is weakened by using a gene transcription inhibition method. | ||||||
| 19 | MICROORGANISM COMPRISING PYRUVATE DEHYDROGENASE VARIANT AND METHOD OF PRODUCING C4-CHEMICALS USING THE SAME | US14472596 | 2014-08-29 | US20150064758A1 | 2015-03-05 | Wooyong Lee; Joonsong Park; Youngmin Lee; Jaechan Park; Jinhwan Park |
| A recombinant microorganism including pyruvate dehydrogenase having increased activity may increase 1,4-BDO production under anaerobic conditions, as well as a method for preparing same, and method of using same to produce a C4 chemical. | ||||||
| 20 | METHODS FOR PRODUCING 3-HYDROXYPROPIONIC ACID AND OTHER PRODUCTS | US13916534 | 2013-06-12 | US20140045231A1 | 2014-02-13 | Michael D. LYNCH; Ryan T. GILL; Tanya E.W. LIPSCOMB |
| This invention relates to metabolically engineered microorganism strains, such as bacterial strains, in which there is an increased utilization of malonyl-CoA for production of a chemical product, which includes 3-hydroxypropionic acid. | ||||||
QQ群二维码
意见反馈
意见反馈