子分类：
| 序号 | 专利名 | 申请号 | 申请日 | 公开（公告）号 | 公开（公告）日 | 发明人 |
|---|---|---|---|---|---|---|
| 201 | Continuous Flow Bioreactor for Magnetically Stabilized Three-Dimensional Tissue Culture | US13583146 | 2011-03-08 | US20130203145A1 | 2013-08-08 | Christopher R. Lambert; W. Grant McGimpsey; Raymond L. Page; Tanja Dominko |
| The invention provides methods for rapid, continuous generation of cells and cell products using magnetically stabilized three-dimensional tissue culture. The invention also pertains to a continuous flow self-regulating closed system bioreactor system for magnetically stabilized three-dimensional tissue culture. The methods described here do not use traditional solid scaffolding for cell culture. | ||||||
| 202 | METHOD FOR INDUCING DIFFERENTIATION OF ADULT STEM CELLS AND NERVE CELLS USING ELECTROMAGNETIC FIELD | US13702975 | 2011-06-08 | US20130202565A1 | 2013-08-08 | Jung-Keug Park; Sung Min Kim; Soo Chan Kim; Moon Young Yoon; Hyun Jin Cho; Young-Kwon Seo; Hee Hoon Yoon |
| The present invention relates to a method for differentiation of mesenchymal stem cells or dental pulp stem cells. More specifically, the invention relates to a method for differentiating stem cells to neural cells by applying mesenchymal stem cells or dental pulp stem cells with a low-frequency electromagnetic field. The differentiation method according to the present invention can induce differentiation even with low-cost mediums rather than induced neural differentiation mediums which are expensive due to addition of growth factors, and the neural cells differentiated according to the present invention may be useful for treatment of neurological brain diseases. | ||||||
| 203 | CULTURE METHOD AND CULTURE DEVICE | US13820907 | 2011-09-08 | US20130171712A1 | 2013-07-04 | Junji Fukuda; Hiroaki Suzuki; Naoto Mochizuki; Takahiro Kakegawa |
| Provided are a culture method and a culture device for efficiently producing cells and/or cell tissue suitable for medication applications. A culture method of culturing cells adhered onto an electrode layer, the method including: a first step of adhering the cells onto the electrode layer and culturing the cells thereon, the electrode layer being coated with an oligopeptide including: a terminal amino acid; a cell adhesive sequence; and an alternating sequence to be bound to the one end side of the cell-adhesive sequence, the alternating sequence including a plurality of acidic amino acids and a plurality of basic amino acids, being alternately bound to each other one by one; and a second step of applying, to the electrode layer having the cells adhered thereonto, an electrical potential inducing reductive desorption of the oligopeptide, to thereby detach the cells from the electrode layer. | ||||||
| 204 | IN SITU TISSUE ENGINEERING USING MAGNETICALLY GUIDED THREE DIMENSIONAL CELL PATTERNING | US13402627 | 2012-02-22 | US20120214217A1 | 2012-08-23 | Shawn Patrick Grogan; Darryl David D'Lima; Clifford W. Colwell, JR.; Sungho Jin |
| Methods are provided for the three dimensional manipulation of cells, and for the formation of an organized engineered cell tissue. Also provided are the organized engineered cell tissues produced by the methods. In one method, a plurality of magnetically labeled cells are mixed with a cross-linkable hydrogel to form a cell-hydrogel mixture, the at least a portion of the plurality of magnetically labeled cells are manipulated with a magnetic field to arrange the magnetically labeled cells into a specific cellular arrangement, and the hydrogel is crosslinked to form the organized engineered cell tissue. The approach presented herein offers a means to circumvent the deficiencies in the field of regenerative medicine, and allows for the production of organized tissues in situ with specific cellular organizations that mimic the native tissue. | ||||||
| 205 | DIFFERENTIATION OF STEM CELLS WITH NANOPARTICLES | US13132060 | 2009-12-01 | US20120076830A1 | 2012-03-29 | Balaji Sitharaman; Longtin Jon |
| A method for differentiating mesenchymal stem cells (MSCs) towards osteoblasts and other connective tissue using nanoparticles and electromagnetic stimulation Osteoinductive materials produced using said method may be useful for bone regeneration and reconstruction in treatment of bone trauma and bone related diseases, and to correct birth defects. | ||||||
| 206 | SYSTEMS AND METHODS FOR MAGNETIC GUIDANCE AND PATTERNING OF MATERIALS | US13070873 | 2011-03-24 | US20110286975A1 | 2011-11-24 | Glauco R. Souza; Renata Pasqualini; Wadih Arap; Thomas Charles Killian; Robert M. Raphael; Daniel Joshua Stark |
| Systems and methods generally useful in medicine, cellular biology, nanotechnology, and cell culturing are discussed. In particular, at least in some embodiments, systems and methods for magnetic guidance and patterning of cells and materials are discussed. Some specific applications of these systems and methods may include levitated culturing of cells away from a surface, making and manipulating patterns of levitated cells, and patterning culturing of cells on a surface. Specifically, a method of culturing cells is presented. The method may comprise providing a plurality of cells, providing a magnetic field, and levitating at least some of the plurality of cells in the magnetic field, wherein the plurality of cells comprise magnetic nanoparticles. The method may also comprise maintaining the levitation for a time sufficient to permit cell growth to form an assembly. | ||||||
| 207 | Process for Preparing Cells for Therapeutic and/or Prophylactic Reimplantation | US11531158 | 2006-09-12 | US20070078507A1 | 2007-04-05 | Fred Zacouto |
| A heart stimulation device that markedly increases the coronary rate instantaneously and durably, causes dilatation of the walls to regress and opposes thromboses and arrhythmia. The device causes genetic involution of pathological processes by affecting the mechanosensitivity of specific genetic expressions or by addition of partial autologous cell dedifferentiation, obtained by original genetic manipulation which induces physiologic and anatomical regeneration. Processes for preparing cells which can be reimplanted prophylactically or therapeutically, wherein a nucleus of a differentiated cell is transported in an oocyte from a homologous or heterologous mammal or human, so as to induce stages of mitosis in the transferred nucleus, wherein this nucleus in mitosis is then removed from the oocyte before its cell division and then this partially dedifferentiated nucleus is introduced into a differentiated receiving cell. Also provided are physiological auto-contractile living arterial stents (e.g., coronary stents) and grafting of dedifferentiated myocardial cells. | ||||||
| 208 | Inotropic Orthorhythmic Cardiac Stimulator | US11381690 | 2006-05-04 | US20060247701A1 | 2006-11-02 | Fred Zacouto |
| Programmable and implantable automatic heart stimulation device (OIST) for controlling the heart, accompanied by a marked increase in the contractility of the myocardial cells on each beat produced by an optimized post-extrasystolic potentiation effect. The OIST does not cause lasting fatigue of the myocardium, markedly increases the coronary rate instantaneously and durably, causes dilatation of the walls to regress and opposes thromboses and arrhythmia. The OIST creates a genetic involution of the pathological process either by the mere effect of the mechanosensitivity of specific genetic expressions or by the addition of partial autologous cell dedifferentiation, obtained by original genetic manipulation which induces physiological an anatomical regeneration. This method also allows physiological auto-contractile living arterial stents, in particular coronary stents to be created, as well as the grafting of dedifferentiated myocardial cells. | ||||||
| 209 | Method and composition for repairing epithelial and other cells and tissue | US11363592 | 2006-02-27 | US20060193837A1 | 2006-08-31 | Donnie Rudd |
| The present invention is directed to the TVEMF-expansion of mammalian blood stem cells, preferably CD34+/CD38− cells, to compositions resulting from the TVEMF-expanded cells, and to a method of treating a disease or condition or repairing tissue of skin, mouth or ear with the compositions. | ||||||
| 210 | Application of electrical stimulation for functional tissue engineering in vitro and in vivo | US10872577 | 2004-06-21 | US20050112759A1 | 2005-05-26 | Milica Radisic; Hyoungshin Park; Robert Langer; Lisa Freed; Gordana Vunjak-Novakovic |
| The present invention provides new methods for the in vitro preparation of bioartificial tissue equivalents and their enhanced integration after implantation in vivo. These methods include submitting a tissue construct to a biomimetic electrical stimulation during cultivation in vitro to improve its structural and functional properties, and/or in vivo, after implantation of the construct, to enhance its integration with host tissue and increase cell survival and functionality. The inventive methods are particularly useful for the production of bioartificial equivalents and/or the repair and replacement of native tissues that contain electrically excitable cells and are subject to electrical stimulation in vivo, such as, for example, cardiac muscle tissue, striated skeletal muscle tissue, smooth muscle tissue, bone, vasculature, and nerve tissue. | ||||||
| 211 | 고강도 전자기장을 이용하여 성체 줄기세포를 신경세포로 분화시키는 방법 | KR1020150046741 | 2015-04-02 | KR101743539B1 | 2017-06-08 | 서영권; 박정극; 윤희훈; 조현진; 박희정; 김유미; 유보영; 조상은; 김상헌 |
| 본발명은 100 내지 1500mT의고강도, 0.01 내지 100Hz의저주파수전자기장을중간엽줄기세포또는성체줄기세포에처리하여, 중간엽줄기세포또는성체줄기세포를신경세포로분화시키는방법에관한것이다. 또한본 발명은상기방법이적용된의료기기에관한것이다. 본발명에따른자기장을이용한신경세포분화방법및 조성물은저주파수의고강도전자기장을이용하여성체줄기세포를신경세포로분화유도함으로써, 짧은시간의전자기장처리만으로신경세포나신경줄기세포를용이하게분화시킬수 있다. | ||||||
| 212 | 종양에 대한 직접적 억제 효과를 갖는 인터페론을 결정하는 방법 및 그의 용도 | KR1020167015609 | 2014-11-12 | KR1020160093637A | 2016-08-08 | 웨이,광웬 |
| 본발명은 rSIFN-co (고형종양에대한치료효과를갖는인터페론)과대비하여시험화합물의효력을결정하거나또는비교하는신규방법; 시험인터페론과 rSIFN-co 사이의실질적동등성을확립하는방법; Wnt-관련수용체또는보조-수용체, 예컨대 LRP6/FZD6의발현의하향조절; Wnt-관련표적유전자, 예컨대 Axin2, CD24, 서바이빈및/또는 ID2의발현의하향조절; 베타-카테닌/TCF 전사활성의억제; 베타-카테닌의발현의억제; 종양억제유전자, 예컨대 DKK-3, BATF2 및/또는 KLF4의상향조절; 시험관내종양세포생존율의억제; 생체내종양성장및 전이의억제; 시험관내종양세포이동, 위족형성및 콜로니형성의억제를위한방법; 뿐만아니라시험인터페론의효력을결정하는방법, 이러한방법의결정을위한키트및 상기활성을갖는인터페론또는인터페론대체물을제공한다. | ||||||
| 213 | 동적으로 구성가능한 신경망에 대한 신경돌기 성장의 전기역학적 구속 | KR1020157021463 | 2014-01-14 | KR1020150110581A | 2015-10-02 | 볼드만,조엘; 호네거,티볼트; 페이레이드,데이빗 |
| 신경성장을변화시키기위한시스템및 방법이일반적으로서술된다. 일부실시예에서, 시스템은신경돌기를포함하는뉴런및 물리적유도신호를생성할수 있는전극을포함할수 있다. 물리적유도신호는신경돌기의성장을변화시키기위해사용될수 있고, 일시적이고공간적으로동적일수 있어서, 신경돌기성장은공간적및/또는일시적방식으로변화될수 있다. 신경돌기성장의동적제어는지향성뉴런연결, 교차및/또는겹침을형성하도록사용될수 있다. 시스템은생세포를보유하고세포성장을촉진할수 있는챔버; 채널; 채널을교차하며전극의중심간격이약 200 마이크론이하인적어도하나의전극쌍을포함하며, 이때이때상기채널은챔버에연결되고, 채널은약 20 마이크론이하의폭 및/또는높이를갖고, 또한복수의전극쌍은채널을교차한다. | ||||||
| 214 | 저선량 방사선을 이용한 손상된 연골세포의 복구방법 | KR1020130164277 | 2013-12-26 | KR101550370B1 | 2015-09-07 | 황상구; 홍은희 |
| 본발명은 2 cGy 이하의선량을나타내는저선량방사선을손상된연골세포에조사하여연골세포의염증반응, 탈분화또는파괴를억제하는방법및 2 cGy 이하의선량을나타내는저선량방사선을손상된연골세포에조사하여연골질환을치료하는방법에관한것이다. 본발명의방법을이용하면, 연골세포에손상을유발하지않는 LDR을이용하여손상된연골을회복시킬수 있으므로, 연골의염증성질환의치료에응용될수 있을것이다. | ||||||
| 215 | 전자기장을 이용한 성체세포를 유도만능 줄기세포로 역분화시키는 방법 | KR1020130124784 | 2013-10-18 | KR101548534B1 | 2015-09-02 | 김종필; 백순봉; 박정극 |
| 본발명은전자기장을이용한성체세포를유도만능줄기세포로역분화시키는방법에관한것이다. 본발명에따른전자기장을이용한유도만능줄기세포로역분화시키는방법은성체세포를유도만능줄기세포로역분화시키는효율이우수하고, 유도만능줄기세포를용이하게수득할수 있다. 또한, 상기방법으로제조된유도만능줄기세포는역분화인자의발현양이높고, 마우스에이식하는경우, 전분화능이나타난다. 따라서, 상기역분화된유도만능줄기세포를세포치료제개발및 재생의학분야의연구등에유용하게사용할수 있다. | ||||||
| 216 | 전자기장을 이용한 성체세포를 유도만능 줄기세포로 역분화시키는 방법 | KR1020130124784 | 2013-10-18 | KR1020150045557A | 2015-04-29 | 김종필; 백순봉; 박정극 |
| 본발명은전자기장을이용한성체세포를유도만능줄기세포로역분화시키는방법에관한것이다. 본발명에따른전자기장을이용한유도만능줄기세포로역분화시키는방법은성체세포를유도만능줄기세포로역분화시키는효율이우수하고, 유도만능줄기세포를용이하게수득할수 있다. 또한, 상기방법으로제조된유도만능줄기세포는역분화인자의발현양이높고, 마우스에이식하는경우, 전분화능이나타난다. 따라서, 상기역분화된유도만능줄기세포를세포치료제개발및 재생의학분야의연구등에유용하게사용할수 있다. | ||||||
| 217 | 전자기장을 이용한 성체 줄기세포의 신경세포 분화유도 방법 | KR1020100101651 | 2010-10-19 | KR101210988B1 | 2012-12-11 | 박정극; 김성민; 김수찬; 윤문영; 조현진; 서영권; 전송희; 윤희훈 |
| 본발명은중간엽줄기세포또는치수줄기세포의분화방법에관한것이다. 보다구체적으로는, 저주파의전자기장을중간엽줄기세포또는치수줄기세포에처리하여, 줄기세포를신경세포로분화시키는방법에관한것이다. 본발명에의한분화방법은성장인자추가에따른고비용의신경분화유도배지가아닌저가의배지에서도분화를유도할수 있으며, 본발명에의해분화된신경세포는뇌신경질환치료에유용하게사용될수 있다. | ||||||
| 218 | 음파를 이용한 중간엽 줄기세포의 신경세포 분화유도 방법 | KR1020100101652 | 2010-10-19 | KR101210984B1 | 2012-12-11 | 박정극; 윤문영; 조현진; 서영권; 전송희; 윤희훈 |
| 본발명은중간엽줄기세포의분화방법에관한것이다. 보다구체적으로는, 저주파의음파를중간엽줄기세포에처리하여, 중간엽줄기세포를신경세포로분화시키는방법에관한것이다. 본발명에의한분화방법은성장인자추가에따른고비용의신경분화유도배지가아닌저가의배지에서도분화를유도할수 있으며, 본발명에의해분화된신경세포는뇌신경질환치료에유용하게사용될수 있다. | ||||||
| 219 | 제대혈로부터 유래한 용이하게 가능한 세포 물질을제공하는 방법 및 그의 조성물 | KR1020077018081 | 2006-01-26 | KR1020070103431A | 2007-10-23 | 러드도니 |
| The present invention is directed to the TVEMF-expansion of mammalian cord blood stem cells, preferably CD34+/CD38-cells, to compositions resulting from the TVEMF-expanded cells, and to a method of treating disease or repairing tissue with the compositions. Various benefits and advantages to the compositions of the present invention are discussed herein. | ||||||
| 220 | SYSTEMS AND METHODS FOR MAGNETIC GUIDANCE AND PATTERNING OF CELLS AND MATERIALS | EP09816948.5 | 2009-09-25 | EP2342338B1 | 2018-11-07 | SOUZA, Glauco, R.; PASQUALINI, Renata; ARAP, Wadih; KILLIAN, Thomas, Charles; RAPHAEL, Robert, M.; STARK, Daniel, Joshua |
| Systems and methods generally useful in medicine, cellular biology, nanotechnology, and cell culturing are discussed. In particular, at least in some embodiments, systems and methods for magnetic guidance and patterning of cells and materials are discussed. Some specific applications of these systems and methods may include levitated culturing of cells away from a surface, making and manipulating patterns of levitated cells, and patterning culturing of cells on a surface. Specifically, a method of culturing cells is presented. The method may comprise providing a plurality of cells, providing a magnetic field, and levitating at least some of the plurality of cells in the magnetic field, wherein the plurality of cells comprise magnetic nanoparticles. The method may also comprise maintaining the levitation for a time sufficient to permit cell growth to form an assembly. | ||||||
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