| 序号 | 专利名 | 申请号 | 申请日 | 公开（公告）号 | 公开（公告）日 | 发明人 |
|---|---|---|---|---|---|---|
| 241 | CELL CULTURE IMPROVEMENTS | US14226333 | 2014-03-26 | US20140206038A1 | 2014-07-24 | Itzcoatl A. Pla; Joseph G. Matuck; John C. Fann; Christof Schulz; Nichole A. Roy; David F. Bruton; James McIntire; Yu-Hsiang David Chang; Thomas Seewoester |
| The invention describes improved methods and compositions for producing a recombinant protein, e.g., an antibody, in mammalian cell culture. In addition, the invention provides improved cell culture media, including improved production media, feed solutions, and combination feeds, which may be used to improve protein productivity in mammalian cell culture. | ||||||
| 242 | CELL CULTURE IMPROVEMENTS | US14156829 | 2014-01-16 | US20140134674A1 | 2014-05-15 | Itzcoatl A. Pla; Joseph C. Matuck; John C. Fann; Christof Schulz; Nichole A. Roy; David F. Bruton; James McIntire; Yu-Hsiang David Chang; Thomas Seewoester |
| The invention describes improved methods and compositions for producing a recombinant protein, e.g., an antibody, in mammalian cell culture. In addition, the invention provides improved cell culture media, including improved production media, feed solutions, and combination feeds, which may be used to improve protein productivity in mammalian cell culture. | ||||||
| 243 | Method for polyclonal immunoglobulin G production by human B cells | US13120508 | 2009-08-17 | US08703486B2 | 2014-04-22 | Sonia Néron; Annie Roy; Jessie Farah Fecteau |
| This application relates to an in vitro method of producing a polyclonal IgG preparation. The method comprises (i) placing a polyclonal B-cell population enriched in IgG-secreting B cells in a culture medium; and (ii) culturing the polyclonal B-cell population under conditions enabling the production of the polyclonal IgG preparation from the polyclonal B-cell population. This improved method enables the production of antibodies (preferably IgG) and facilitates long-term culture of polyclonal B-cell populations. | ||||||
| 244 | METHODS TO CONTROL PROTEIN HETEROGENEITY | US13804220 | 2013-03-14 | US20140065710A1 | 2014-03-06 | Lisa M. Rives; Cornelia Bengea; Xiaobei Zeng |
| The instant invention relates to the field of protein production, and in particular to compositions and processes for controlling and limiting the heterogeneity of proteins expressed in host cells. | ||||||
| 245 | Methods of producing anti-TNF-alpha antibodies in mammalian cell culture | US13308075 | 2011-11-30 | US08663945B2 | 2014-03-04 | Itzcoatl A. Pla; Joseph C. Matuck; John C. Fann; Christof Schulz; Nicole A. Roy; David F. Bruton; James McIntire; David Chang Yu-Hsiang; Thomas Seewoester |
| The invention describes improved methods and compositions for producing a recombinant protein, e.g., an antibody, in mammalian cell culture. In addition, the invention provides improved cell culture media, including improved production media, feed solutions, and combination feeds, which may be used to improve protein productivity in mammalian cell culture. | ||||||
| 246 | METHODS FOR CULTURING HUMAN MYELOID LEUKAEMIA CELLS AND CELLS DERIVED THEREFROM | US13995730 | 2011-12-21 | US20130330768A1 | 2013-12-12 | Rainer Stahn |
| The present invention pertains to a method for culturing a suspension of immortalized human blood cells, preferably cells of myeloid leukaemia origin or cells derived therefrom, wherein said method provides a high productivity, a high cell viability and growth rate and a high batch-to-batch consistency, and can be scaled up without altering these parameters. | ||||||
| 247 | METHODS FOR DEVELOPING ANTIGEN-SPECIFIC ANTIBODY-PRODUCING CELL LINES AND MONOCLONAL ANTIBODIES | US13531957 | 2012-06-25 | US20130177987A1 | 2013-07-11 | Brian Schram; Rachel Kravitz |
| Disclosed are methods for producing class-switched, affinity-matured antibodies which include enriching an immunized cell population for GL7-positive cells and activating the enriched cells. The methods may be used to improve the efficiency of obtaining immortalized antigen-specific plasma cells or to improve the quality of molecularly cloned Ig heavy and light chains. | ||||||
| 248 | CELL CULTURE SYSTEM FOR BIOREACTOR SCALE-UP OF CELLS | US13820462 | 2011-09-02 | US20130164269A1 | 2013-06-27 | Andrew Mark Campbell; Lucas Chase; Mohanachari Vemuri |
| The present invention relates to growing stem cells, for e.g., MSCs, in large-scale under GMP-compliance, using media and reagents that satisfy GMP requirements, while maintaining stemness, for effective downstream therapeutic use, which include but are not limited to, stem cell therapy, production of products, such as beneficial factors, recombinant proteins, etc. obtained from such stem cells. | ||||||
| 249 | SCALABLE PRIMATE PLURIPOTENT STEM CELL AGGREGATE SUSPENSION CULTURE AND DIFFERENTIATION THEREOF | US13672688 | 2012-11-08 | US20130115695A1 | 2013-05-09 | Thomas C. Schulz |
| The present invention relates to methods for production of undifferentiated or differentiated embryonic stem cell aggregate suspension cultures from undifferentiated or differentiated embryonic stem cell single cell suspensions and methods of differentiation thereof. | ||||||
| 250 | NANOPARTICLE-LOADED CELLS | US13267374 | 2011-10-06 | US20120087868A1 | 2012-04-12 | Gabriele Todd; Alla Danilkovitch |
| The invention provides nanoparticle-loaded cells and compositions useful for improved imaging and therapy, for example radio-therapy. The invention also provides methods of manufacture of nanoparticle-loaded cells, methods of administering the nanoparticle-loaded cells, and methods for treatment and/or imaging. | ||||||
| 251 | CELL CULTURE IMPROVEMENTS | US13308075 | 2011-11-30 | US20120077213A1 | 2012-03-29 | Itzcoatl A. Pla; Joseph G. Matuck; John C. Fann; Christof Schulz; Nichole A. Roy; David F. Bruton; James McIntire; Yu-Hsiang David Chang; Thomas Seewoester |
| The invention describes improved methods and compositions for producing a recombinant protein, e.g., an antibody, in mammalian cell culture. In addition, the invention provides improved cell culture media, including improved production media, feed solutions, and combination feeds, which may be used to improve protein productivity in mammalian cell culture. | ||||||
| 252 | METHOD OF PRODUCING RECOMBINANT ADAMTS13 IN CELL CULTURE | US13179399 | 2011-07-08 | US20120034674A1 | 2012-02-09 | Leopold Grillberger; Manfred Reiter; Daniel Fleischanderl; Gregor Bramberger |
| Among other aspects, the present invention relates to cell culture conditions for producing high molecular weight vWF, in particular, highly multimericWF with a high specific activity and ADAMTS13 with a high specific activity. The cell culture conditions of the present invention can include, for example, a cell culture medium with an increased copper concentration and/or cell culture supernatant with a low ammonium (NH4+) concentration. The present invention also provides methods for cultivating cells in the cell culture conditions to express high molecular weight vWF and rA13 having high specific activities. | ||||||
| 253 | MULTI-FUNCTIONAL CHAMBER FOR HOUSING A BIOLOGICAL COMPONENT | US12667323 | 2008-06-25 | US20110125286A1 | 2011-05-26 | Clare Selden; Humphrey Hodgson; Sam Coward |
| The present invention relates to the field of extracorporeal liver perfusion and, more particularly, to the design of a chamber in which a biological component can be housed to form e.g. a bio-artificial liver (BAL). It also relates to a bio-artificial liver per se, it's components and methodological steps associated with its development and use. The chamber (10) for the biological component (100) of a bio-artificial liver (200) is configured to allow: • Proliferation of the biological component, in situ; • Cryopreservation of the biological component, in situ, and • Perfusion of the biological component, in situ. | ||||||
| 254 | Serum-Free Culture Medium for the Production of Recombinant Gonadotropins | US11994885 | 2006-07-04 | US20090124006A1 | 2009-05-14 | Jean-Pierre Fonta; Paul Ducommun; Veronique Deparis |
| The present invention is in the field of the manufacture of recombinant proteins. More specifically, it relates to the use of a serum-free culture medium comprising an antioxidant for the production of recombinant dimeric gonadotropins. The antioxidant may be selected from the group consisting of L-glutathione, 2-mercaptoethanol, L-methionine and a combination of ascorbic acid and of (+)-alpha-tocoplierol. | ||||||
| 255 | Cell culture improvements | US11901274 | 2007-09-13 | US20080227136A1 | 2008-09-18 | Itzcoatl A. Pla; Joseph G. Matuck; John C. Fann; Christof Schulz; Nichole A. Roy; David F. Bruton; James McIntire; Yu-Hsiang David Chang; Thomas Seewoester |
| The invention describes improved methods and compositions for producing a recombinant protein, e.g., an antibody, in mammalian cell culture. In addition, the invention provides improved cell culture media, including improved production media, feed solutions, and combination feeds, which may be used to improve protein productivity in mammalian cell culture. | ||||||
| 256 | METHOD FOR PREPARING AQUEOUS SOLUTION CONTAINING CULTURE MEDIUM AND CHELATING AGENT | EP11854382.6 | 2011-12-27 | EP2660315B1 | 2018-11-14 | KONNO, Yoshinobu; WAKAMATSU, Kairo; IMAMOTO, Yasufumi; ISHIBASHI, Jun; TAKAHASHI, Ken; TANAKA, Hisaya |
| Provided are a method for preparing a highly versatile aqueous solution having remarkably improved membrane filterability, which can be stably membrane-filtered in a short time, an aqueous solution prepared by the preparation method, a method for culturing cells using the aqueous solution which is prepared by the preparation method, a method for producing a physiologically active substance using the culturing method, a physiologically active substance produced by the production method of the aqueous solution, a method for performing membrane filtration of the aqueous solution which is prepared by the preparation method of the aqueous solution, a method for improving membrane filterability of the aqueous solution, and a method for producing the physiologically active substance by preparing the aqueous solution, performing membrane filtration of the aqueous solution, and then culturing cells using the resulting aqueous solution. The present invention relates to a method for preparing an aqueous solution, characterized by addition of a chelating agent. | ||||||
| 257 | CELL CULTURE SYSTEM FOR BIOREACTOR SCALE-UP OF CELLS | EP11758028.2 | 2011-09-02 | EP2611452B1 | 2018-10-24 | CAMPBELL, Andrew; CHASE, Lucas; VEMURI, Mohanachari |
| The present invention relates to growing stem cells, for e.g., MSCs, in large-scale under GMP-compliance, using media and reagents that satisfy GMP requirements, while maintaining stemness, for effective downstream therapeutic use, which include but are not limited to, stem cell therapy, production of products, such as beneficial factors, recombinant proteins, etc. obtained from such stem cells. | ||||||
| 258 | SERUM-FREE CELL CULTURE MEDIUM | EP18172141.6 | 2014-03-14 | EP3378932A1 | 2018-09-26 | OSHODI, Shadia; JOHNSON, Amy; LAWRENCE, Shawn |
The specification describes an improved serum-free animal cell culture medium, which can be used for the production of a protein of interest. Omithine, or a combination of omithine and putrescine can be added to serum-free media or chemically defined media to improve viable cell density, to reduce cell doubling time, and to increase the production of a protein of interest. |
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| 259 | METHODS FOR MODULATING PRODUCTION PROFILES OF RECOMBINANT PROTEINS | EP16736163.3 | 2016-07-07 | EP3325609A1 | 2018-05-30 | MUHR, Anaïs; BRUHLMANN, David; JORDAN, Martin; BROLY, Hervé; STETTLER, Matthieu |
| The invention is in the field of cell culture. Particularly the invention relates to methods of culturing a host cell expressing a recombinant protein in a cell culture medium comprising an effective amount of 4,4′-diisothiocyanostilbene-2,2′-disulfonic acid (DIDS) or supplemented with an effective amount of DIDS, whereby production of said protein is increased relative to cells grown without DIDS. | ||||||
| 260 | METABOLICALLY OPTIMIZED CELL CULTURE | EP14851841.8 | 2014-10-10 | EP3055409B1 | 2018-04-25 | LAWRENCE, Shawn; KIM, Ann; JOHNSON, Amy |
| An improved method for large scale production of proteins and/or polypeptides in cell culture is provided. In accordance with the present invention, the method provides for culturing cells that have metabolically shifted. The use of such a method or system allows high levels of protein or polypeptide production and reduces accumulation of unwanted metabolic waste such as lactate. Proteins and polypeptides expressed in accordance with the present invention may be advantageously used in the preparation of pharmaceutical, immunogenic, or other commercial biologic compositions, such as antibodies. | ||||||
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