| 序号 | 专利名 | 申请号 | 申请日 | 公开（公告）号 | 公开（公告）日 | 发明人 |
|---|---|---|---|---|---|---|
| 201 | Method of producing recombinant high molecular weight vWF in cell culture | US15591022 | 2017-05-09 | US10100099B2 | 2018-10-16 | Leopold Grillberger; Manfred Reiter; Daniel Fleischanderl; Gregor Bramberger |
| Among other aspects, the present invention relates to cell culture conditions for producing high molecular weight vWF, in particular, highly multimericWF with a high specific activity and ADAMTS13 with a high specific activity. The cell culture conditions of the present invention can include, for example, a cell culture medium with an increased copper concentration and/or cell culture supernatant with a low ammonium (NH4+) concentration. The present invention also provides methods for cultivating cells in the cell culture conditions to express high molecular weight vWF and rA13 having high specific activities. | ||||||
| 202 | CELL CULTURE METHODS AND SYSTEMS | US15577991 | 2016-05-27 | US20180291329A1 | 2018-10-11 | Justin Moretto; Thomas Matthews; Brandon Moore; Terrence Dobrowsky; An Zhang; John Smelko; Brandon Berry; Kelly Wiltberger |
| The present disclosure relates, in some aspects, to the field of process analytical technology with respect to biotherapeutic protein production processes and products. | ||||||
| 203 | CELLS AND METHOD OF CELL CULTURE | US15762345 | 2016-09-22 | US20180265904A1 | 2018-09-20 | GREGORY WALTER HILLER; JEFFREY JOSEPH MITCHELL; BHANU CHANDRA MULUKUTLA; PAMELA MARY PEGMAN |
| The invention relates to a method of cell culture where the cells are modified to reduce the level of synthesis of growth and/or productivity inhibitors by the cell. The invention also relates to a method of cell culture for improving cell growth and productivity, in particular in fed-batch culture of mammalian cells at high cell density. The invention further relates to a method of producing cells with improved cell growth and/or productivity in cell culture and to cells obtained or obtainable by such methods. | ||||||
| 204 | MANUFACTURING METHOD OF AN IMMUNOTHERAPEUTIC FORMULATION COMPRISING A RECOMBINANT LISTERIA STRAIN | US15760263 | 2016-09-13 | US20180265879A1 | 2018-09-20 | Anu Wallecha; Yvonne Paterson |
| The present invention discloses a process for manufacturing a formulation comprising a drug substance, said drug substance comprising a recombinant Listeria comprising a human papilloma virus (HPV) antigen fused to a Listeriolysin O (LLO) protein fragment. The invention further discloses methods of using treating, protecting against, and inducing an immune response against cervical cancer comprising administration of the recombinant Listeria strain. The present invention also provides methods for inducing an anti-E7 CTL response in a human subject and treating HPV-mediated diseases, disorders, and symptoms, comprising administration of the recombinant Listeria strain. | ||||||
| 205 | Cell culture media and methods | US15198237 | 2016-06-30 | US10066200B2 | 2018-09-04 | Richard Fike; Shawn Barrett; Zhou Jiang; David Judd |
| Compositions and methods are described for preparing media, feeds, and supplements. Such methods and medias may display increased stability of labile components and may use, for example, microsuspension and/or encapsulation technologies, chelation, and optionally, coating and/or mixing the labile compounds with anti-oxidants. The compositions may withstand thermal and/or irradiation treatment and have reduced virus number. These techniques may result in product with extended shelf-life, extended release of their internal components into culture, or in product that can be added aseptically into a bioreactor using minimal volumes. The compositions and methods may optimize the bioproduction workflow and increase efficiency. | ||||||
| 206 | METHODS FOR MODULATING PRODUCTION PROFILES OF RECOMBINANT PROTEINS | US15743714 | 2016-07-07 | US20180201897A1 | 2018-07-19 | ANAIS MUHR; DAVID BRUHLMANN; MARTIN JORDAN; HERVE BROLY; MATTHIEU STETTLER |
| The invention is in the field of cell culture. Particularly the invention relates to methods of culturing a host cell expressing a recombinant protein in a cell culture medium comprising an effective amount of a triazine dye or supplemented with an effective amount of a triazine dye, whereby production of said protein is increased relative to cells grown without said triazine dye or any other inducer. | ||||||
| 207 | Enhancing ingredients for protein production from various cells | US13974820 | 2013-08-23 | US10017805B2 | 2018-07-10 | Misayo Tomura; Takehisa Iwama; Koichiro Saruhashi; Taito Nishino; Masato Horikawa; Hiroharu Kawahara |
| The present invention relates to a protein production accelerating agent that has enabled to largely increase the produced amount of a desired protein by adding polysaccharides to a medium for animal cells containing a serum or serum alternative, and a production method of a protein using a medium containing the protein production accelerating agent. | ||||||
| 208 | BIOLOGICAL METHODS FOR PREPARING 3-HYDROXYPROPIONIC ACID | US15558863 | 2016-03-18 | US20180148744A1 | 2018-05-31 | Eric Michael KNIGHT |
| This technology relates in part to biological methods for producing 3-hydroxypropionic acid and engineered microorganisms capable of such production. | ||||||
| 209 | DAC HYP COMPOSITIONS AND METHODS | US15724443 | 2017-10-04 | US20180127506A1 | 2018-05-10 | Taymar E. HARTMAN; Paul W. SAUER; John E. BURKY; Mark C. WESSON; Ping Y. HUANG; Thomas J. ROBINSON; Braeden D. PARTRIDGE; J. Yun TSO |
| The present disclosure relates to compositions of daclizumab suitable for subcutaneous administration and methods of manufacturing thereof. | ||||||
| 210 | Control of protein glycosylation by culture medium supplementation and cell culture process parameters | US15000522 | 2016-01-19 | US09944968B2 | 2018-04-17 | William Yang; Yao-Ming Huang; Kyle McElearney; Lia Tescione; James Lambropoulos; An Zhang; Valerie Tsang; Thomas Ryll; Sangil Lee; Dae Sung Lee |
| The present invention pertains to a cell culture medium comprising media supplements that are shown to control recombinant protein glycosylation and/or cell culture in a controlled or modulated (shifted) temperature to control recombinant protein glycosylation and/or cell culture with controlled or modulated seed density to control recombinant protein glycosylation, and methods of using thereof. The present invention further pertains to a method of controlling or manipulating glycosylation of a recombinant protein of interest in a large scale cell culture. | ||||||
| 211 | N-GLYCOSYLATION | US15534735 | 2015-12-11 | US20170369905A1 | 2017-12-28 | Henrik Clausen; Zhang Yang; Adnan Fevzi Halim; Eric Bennett; Carsten Behrens; Malene Bech Vester-Christensen; Shamim Herbert Rahman |
| The present invention relates to a mammalian cell comprising a gene encoding a polypeptide of interest, wherein the polypeptide of interest is expressed comprising one or more posttranslational modification patterns. These modifications are useful for example in glycoprotein production where the antibodies with the modifications have an enhanced antibody-dependent cell-mediated cytotoxicity (ADCC). The present invention also relates to methods for producing the glycoproteins and compositions comprising the glycoproteins, and their uses. | ||||||
| 212 | Isolation, expansion and characterization of precursor/stem cells from dental tissues | US14831794 | 2015-08-20 | US09839654B2 | 2017-12-12 | Vijayendran Govindasamy; Premasangery Kathivaloo |
| A method for isolating and proliferating at least one type of precursor cell from dental origin from a single donor includes the following isolating the precursor cells from dental origin of a single donor in a sample collection media and preparing a primary stock culture. The primary stock culture is proliferated sequentially to obtain first, second and third sub-cultured stocks with cell counts ranging between 5×106 cells and 10×106 cells, 20×106 cells and 400×106 cells, 150×106 and 300×106 cells respectively. The precursor cells from the third subculture are harvested and cryo-preserved to obtain a precursor cell population for cell transplantation. The dental origin of the precursor cells is from pulp, apical papilla or periodontal ligament. The precursor cell originates from mesenchymal stem cells, ecto-mesenchymal cells, neural stem cells, dental progenitor cells or CD117+ cells. | ||||||
| 213 | Method of producing recombinant high molecular weight vWF in cell culture | US15201125 | 2016-07-01 | US09834591B2 | 2017-12-05 | Leopold Grillberger; Manfred Reiter; Wolfgang Mundt |
| Among other aspects, the present invention relates to cell culture conditions for producing high molecular weight vWF, in particular, highly multimericWF with a high specific activity and ADAMTS13 with a high specific activity. The cell culture conditions of the present invention can include, for example, a cell culture medium with an increased copper concentration and/or cell culture supernatant with a low ammonium (NH4+) concentration. The present invention also provides methods for cultivating cells in the cell culture conditions to express high molecular weight vWF and rA13 having high specific activities. | ||||||
| 214 | Hypotaurine, GABA, Beta-Alanine, and Choline for Control of Waste Byproduct Accumulation in Mammalian Cell Culture Process | US15523009 | 2015-10-30 | US20170327786A1 | 2017-11-16 | Yao-Ming HUANG; William C. YANG; Alan GILBERT; Kyle MCELEARNEY |
| The present invention pertains to a cell culture medium comprising hypotaurine, GABA, and/or beta-alanine or the combination of choline and hypotaurine, GABA, and/or beta-alanine as media supplements which is shown to control viability, growth, and waste byproduct accumulation. The present invention further pertains to a method of producing a polypeptide of interest in a large scale cell culture containing hypotaurine, GABA, and/or beta-alanine or the combination of choline and hypotaurine, GABA, and/or beta-alanine. | ||||||
| 215 | METHODS TO CONTROL PROTEIN HETEROGENEITY | US15417426 | 2017-01-27 | US20170137496A1 | 2017-05-18 | Lisa M. Rives; Cornelia Bengea; Xiaobei Zeng |
| The instant invention relates to the field of protein production, and in particular to compositions and processes for controlling and limiting the heterogeneity of proteins expressed in host cells. | ||||||
| 216 | MAMMALIAN CELL CULTURE PROCESSES FOR PROTEIN PRODUCTION | US15366218 | 2016-12-01 | US20170129936A1 | 2017-05-11 | Ying JING; Zhengjian Li; Yueming Qian |
| The present invention describes methods and processes for the production of proteins, particularly glycoproteins, by animal cell or mammalian cell culture, preferably, but not limited to, fed-batch cell cultures. In one aspect, the methods comprise the addition of glucocorticoid compound during the culturing period. The addition of glucocorticoid compound sustain a high viability of the cultured cells, and can yield an increased end titer of protein product, and a high quality of protein product, as determined, e.g., by sialic acid content of the produced protein. | ||||||
| 217 | Method for preparing aqueous solution containing culture medium and chelating agent | US13976524 | 2011-12-27 | US09611458B2 | 2017-04-04 | Yoshinobu Konno; Kairo Wakamatsu; Yasufumi Imamoto; Jun Ishibashi; Ken Takahashi; Hisaya Tanaka |
| Provided are a method for preparing a highly versatile aqueous solution having remarkably improved membrane filterability, which can be stably membrane-filtered in a short time, an aqueous solution prepared by the preparation method, a method for culturing cells using the aqueous solution which is prepared by the preparation method, a method for producing a physiologically active substance using the culturing method, a physiologically active substance produced by the method for producing a physiologically active substance, a method for performing membrane filtration of the aqueous solution which is prepared by the preparation method of the aqueous solution, a method for improving membrane filterability of the aqueous solution, and a method for producing the physiologically active substance by preparing the aqueous solution, performing membrane filtration of the aqueous solution, and then culturing cells using the resulting aqueous solution. The present invention relates to a method for preparing an aqueous solution, characterized by addition of a chelating agent. | ||||||
| 218 | Method of Continuous Mass Production of Progenitor Stem-like Cells Using a Bioreactor System | US15140578 | 2016-04-28 | US20170067019A1 | 2017-03-09 | Timothy Ray Ho |
| Disclosed herein is a method of culturing cells for cell therapy in a bioreactor. | ||||||
| 219 | Seeding An Adherent Cell Bioreactor With Non-Adherent Cells Increases Seeding Density Limit And Reduces Required Expansion Time | US15306830 | 2015-08-26 | US20170051309A1 | 2017-02-23 | Hanna P. LESCH; Nigel PARKER; Minna KARHINEN; Robert SHAW; Seppo YLA-HERTTUALA; Jonas MALINEN; Eevi LIPPONEN |
| We have found a counter-intuitive way to improve the commercial-scale production of recombinant biological products in adherent-cell bioreactors, which reduces the risk of cell culture contamination, increases total yield and reduces the delay between seeding and harvest, thus minimizing expression product degradation, by inter alia inoculating an adherent culture bioreactor with suspension-adapted producer cells | ||||||
| 220 | METHODS FOR CONTROLLING THE GALACTOSYLATION PROFILE OF RECOMBINANTLY-EXPRESSED PROTEINS | US15345953 | 2016-11-08 | US20170051052A1 | 2017-02-23 | Cornelia Bengea; Lisa M. Rives; Patrick Hossler |
| The present invention relates to methods for modulating the glycosylation profile of recombinantly-expressed proteins. In particular, the present invention relates to methods of controlling the galactosylation profile of recombinantly-expressed proteins by supplementing production medium, e.g., a hydrolysate-based or a chemically defined medium, with manganese and/or D-galactose | ||||||
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