| 序号 | 专利名 | 申请号 | 申请日 | 公开（公告）号 | 公开（公告）日 | 发明人 |
|---|---|---|---|---|---|---|
| 1 | 新型腔肠素底物及其使用方法 | CN201180063659.6 | 2011-11-02 | CN103443121B | 2016-04-20 | B·宾科夫斯基; L·P·昂塞尔; M·哈尔; M·B·罗贝斯; M·R·斯莱特; K·V·伍德; M·G·伍德 |
| 一种编码修饰的荧光素酶多肽和底物的分离的多核苷酸。OgLuc变体多肽与SEQ ID NO:1具有至少60%氨基酸序列同一性且在对应于SEQ ID NO:1中的氨基酸的位置处具有至少一个氨基酸置换。OgLuc变体多肽相对于野生型刺虾属荧光素酶的对应的多肽具有增强的发光、增强的信号稳定性和增强的蛋白质稳定性中的至少一项。 | ||||||
| 2 | 新型腔肠素底物及其使用方法 | CN201180063659.6 | 2011-11-02 | CN103443121A | 2013-12-11 | B·宾科夫斯基; L·P·昂塞尔; M·哈尔; M·B·罗贝斯; M·R·斯莱特; K·V·伍德; M·G·伍德 |
| 一种编码修饰的荧光素酶多肽和底物的分离的多核苷酸。OgLuc变体多肽与SEQ ID NO:1具有至少60%氨基酸序列同一性且在对应于SEQ ID NO:1中的氨基酸的位置处具有至少一个氨基酸置换。OgLuc变体多肽相对于野生型刺虾属荧光素酶的对应的多肽具有增强的发光、增强的信号稳定性和增强的蛋白质稳定性中的至少一项。 | ||||||
| 3 | 新型腔肠素底物及其使用方法 | CN201180062485.1 | 2011-11-02 | CN103370319B | 2016-08-24 | D.H.克劳伯特; P.梅森黑默; J.安奇 |
| 一种编码修饰的荧光素酶多肽的分离的多核苷酸和新型的基于腔肠素的底物。OgLuc变体多肽与SEQ ID NO：1具有至少60％氨基酸序列同一性且在对应于SEQ ID NO：1中的氨基酸的位置处具有至少一个氨基酸置换。OgLuc变体多肽相对于野生型刺虾属荧光素酶的对应的多肽具有增强的发光、增强的信号稳定性和增强的蛋白质稳定性中的至少一项。 | ||||||
| 4 | 新型腔肠素底物及其使用方法 | CN201180062485.1 | 2011-11-02 | CN103370319A | 2013-10-23 | D.H.克劳伯特; P.梅森黑默; J.安奇 |
| 一种编码修饰的荧光素酶多肽的分离的多核苷酸和新型的基于腔肠素的底物。OgLuc变体多肽与SEQ ID NO：1具有至少60％氨基酸序列同一性且在对应于SEQ ID NO：1中的氨基酸的位置处具有至少一个氨基酸置换。OgLuc变体多肽相对于野生型刺虾属荧光素酶的对应的多肽具有增强的发光、增强的信号稳定性和增强的蛋白质稳定性中的至少一项。 | ||||||
| 5 | IMIDAZO[1,2-alpha]PYRAZINE DERIVATIVES | US14859715 | 2015-09-21 | US20160002703A1 | 2016-01-07 | Dieter H. Klaubert; Poncho Meisenheimer; James Unch |
| The present invention provides, among other things, imidazo[1,2-α]pyrazine derivatives. The derivatives are useful in any method which other coelenterazines have been used. For example, the derivatives may be used in a bioluminogenic method to detect the presence of certain compounds or molecules. | ||||||
| 6 | Oplophorus-derived luciferases, novel coelenterazine substrates, and methods of use | US14160282 | 2014-01-21 | US09951373B2 | 2018-04-24 | Brock Binkowski; Lance P. Encell; Mary Hall; Matthew B. Robers; Michael R. Slater; Keith V. Wood; Monika G. Wood |
| An isolated polynucleotide encoding a modified luciferase polypeptide and substrates. The OgLuc variant polypeptide has at least 60% amino acid sequence identity to SEQ ID NO: 1 and at least one amino acid substitution at a position corresponding to an amino acid in SEQ ID NO: 1. The OgLuc variant polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the corresponding polypeptide of the wild-type Oplophorus luciferase. | ||||||
| 7 | オキヒオドシエビ属由来ルシフェラーゼ、新規セレンテラジン基質、及び使用法 | JP2016024952 | 2016-02-12 | JP6374420B2 | 2018-08-15 | ビンコウスキー ブロック; エンセル ランス ピー; ホール メアリー; ロバース マシュー ビー; スレーター マイケル アール; ウッド キース ヴイ; ウッド モニカ ジー; クラウベルト ディーター; マイゼンハイマー ポンチョ; アンク ジェイムス; ヴァリー マイケル ピー |
| 8 | OPLOPHORUS-DERIVED LUCIFERASES, NOVEL COELENTERAZINE SUBSTRATES, AND METHODS OF USE | EP11785534.6 | 2011-11-02 | EP2635595A2 | 2013-09-11 | BINKOWSKI, Brock; ENCELL, Lance, P.; HALL, Mary; ROBERS, Matthew, B.; SLATER, Michael, R.; WOOD, Keith, V.; WOOD, Monika, G. |
| An isolated polynucleotide encoding a modified luciferase polypeptide and substrates. The OgLuc variant polypeptide has at least 60% amino acid sequence identity to SEQ ID NO: 1 and at least one amino acid substitution at a position corresponding to an amino acid in SEQ ID NO: 1. The OgLuc variant polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the corresponding polypeptide of the wild-type Oplophorus luciferase. | ||||||
| 9 | NOVEL COELENTERAZINE SUBSTRATES AND METHODS OF USE | EP11782732.9 | 2011-11-02 | EP2635582B1 | 2016-01-27 | KLAUBERT, Dieter, H.; MEISENHEIMER, Poncho; UNCH, James |
| An isolated polynucleotide encoding a modified luciferase polypeptide and substrates. The OgLuc variant polypeptide has at least 60% amino acid sequence identity to SEQ ID NO: 1 and at least one amino acid substitution at a position corresponding to an amino acid in SEQ ID NO: 1. The OgLuc variant polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the corresponding polypeptide of the wild-type Oplophorus luciferase. | ||||||
| 10 | NOVEL COELENTERAZINE SUBSTRATES AND METHODS OF USE | EP11782732.9 | 2011-11-02 | EP2635582A1 | 2013-09-11 | KLAUBERT, Dieter, H.; MEISENHEIMER, Poncho; UNCH, James |
| An isolated polynucleotide encoding a modified luciferase polypeptide and substrates. The OgLuc variant polypeptide has at least 60% amino acid sequence identity to SEQ ID NO: 1 and at least one amino acid substitution at a position corresponding to an amino acid in SEQ ID NO: 1. The OgLuc variant polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the corresponding polypeptide of the wild-type Oplophorus luciferase. | ||||||
| 11 | OPLOPHORUS-DERIVED LUCIFERASES, NOVEL COELENTERAZINE SUBSTRATES, AND METHODS OF USE | US15948070 | 2018-04-09 | US20180327810A1 | 2018-11-15 | Brock Binkowski; Lance P. Encell; Mary Hall; Matthew B. Robers; Michael R. Slater; Keith V. Wood; Monika G. Wood |
| An isolated polynucleotide encoding a modified luciferase polypeptide and substrates. The OgLuc variant polypeptide has at least 60% amino acid sequence identity to SEQ ID NO: 1 and at least one amino acid substitution at a position corresponding to an amino acid in SEQ ID NO: 1. The OgLuc variant polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the corresponding polypeptide of the wild-type Oplophorus luciferase. | ||||||
| 12 | Substituted imidazo[1,2-a]pyrazines for use in bioluminogenic methods | US14859715 | 2015-09-21 | US09938564B2 | 2018-04-10 | Dieter H. Klaubert; Poncho Meisenheimer; James Unch |
| The present invention provides, among other things, imidazo[1,2-α]pyrazine derivatives including The derivatives are useful in any method which other coelenterazines have been used. For example, the derivatives may be used in a bioluminogenic method to detect the presence of certain compounds or molecules. | ||||||
| 13 | Oplophorus-derived luciferases, novel coelenterazine substrates, and methods of use | US14160278 | 2014-01-21 | US09840730B2 | 2017-12-12 | Brock Binkowski; Lance P. Encell; Mary Hall; Matthew B. Robers; Michael R. Slater; Keith V. Wood; Monika G. Wood |
| An isolated polynucleotide encoding a modified luciferase polypeptide and substrates. The OgLuc variant polypeptide has at least 60% amino acid sequence identity to SEQ ID NO: 1 and at least one amino acid substitution at a position corresponding to an amino acid in SEQ ID NO: 1. The OgLuc variant polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the corresponding polypeptide of the wild-type Oplophorus luciferase. | ||||||
| 14 | Imidazo[1,2-a]pyrazine derivatives | US14461610 | 2014-08-18 | US09139836B2 | 2015-09-22 | Dieter H. Klaubert; Poncho Meisenheimer; James Unch |
| The present invention provides, among other things, imidazo[1,2-α]pyrazine derivatives according for Formula (Ia) or Formula (Ib). The derivatives are useful in any method which other coelenterazines have been used. For example, the derivatives may be used in a bioluminogenic method to detect the presence of certain compounds or molecules. | ||||||
| 15 | OPLOPHORUS-DERIVED LUCIFERASES, NOVEL COELENTERAZINE SUBSTRATES, AND METHODS OF USE | US14461610 | 2014-08-18 | US20150064731A1 | 2015-03-05 | Dieter H. Klaubert; Poncho Meisenheimer; James Unch |
| The present invention provides, among other things, imidazo[1,2-α]pyrazine derivatives according for Formula (Ia) or Formula (Ib). The derivatives are useful in any method which other coelenterazines have been used. For example, the derivatives may be used in a bioluminogenic method to detect the presence of certain compounds or molecules. | ||||||
| 16 | Imidazo[1,2-α]pyrazine derivatives | US13287992 | 2011-11-02 | US08809529B2 | 2014-08-19 | Dieter H. Klaubert; Poncho Meisenheimer; James Unch |
| The present invention provides, among other things, imidazo[1,2-α]pyrazine derivatives according for Formula (Ia) or Formula (Ib). The derivatives are useful in any method which other coelenterazines have been used. For example, the derivatives may be used in a bioluminogenic method to detect the presence of certain compounds or molecules. | ||||||
| 17 | OPLOPHORUS-DERIVED LUCIFERASES, NOVEL COELENTERAZINE SUBSTRATES, AND METHODS OF USE | US14160278 | 2014-01-21 | US20140227759A1 | 2014-08-14 | Brock Binkowski; Lance P. Encell; Mary Hall; Matthew B. Robers; Michael R. Slater; Keith V. Wood; Monika G. Wood |
| An isolated polynucleotide encoding a modified luciferase polypeptide and substrates. The OgLuc variant polypeptide has at least 60% amino acid sequence identity to SEQ ID NO: 1 and at least one amino acid substitution at a position corresponding to an amino acid in SEQ ID NO: 1. The OgLuc variant polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the corresponding polypeptide of the wild-type Oplophorus luciferase. | ||||||
| 18 | NOVEL COELENTERAZINE SUBSTRATES AND METHODS OF USE | US13287992 | 2011-11-02 | US20120117667A1 | 2012-05-10 | Dieter H. Klaubert; Poncho Meisenheimer; James Unch |
| An isolated polynucleotide encoding a modified luciferase polypeptide and novel coelenterazine-based substrates. The OgLuc variant polypeptide has at least 60% amino acid sequence identity to SEQ ID NO: 1 and at least one amino acid substitution at a position corresponding to an amino acid in SEQ ID NO: 1. The OgLuc variant polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the corresponding polypeptide of the wild-type Oplophorus luciferase. | ||||||
| 19 | 新規セレンテラジン基質及び使用法 | JP2018040100 | 2018-03-06 | JP2018127459A | 2018-08-16 | クラウバート ディーター エイチ; マイゼンハイマー ポンチョ; アンク ジェームズ |
| 【課題】新規セレンテラジン基質及び使用法を提供する。 【解決手段】改変されたルシフェラーゼポリペプチドおよび新規のセレンテラジンベースの基質をコードする単離ポリヌクレオチド。OgLuc変異体ポリペプチドは、配列番号1に対し少なくとも60%のアミノ酸配列同一性、および配列番号1中のアミノ酸に対応する位置に少なくとも1つのアミノ酸置換を有する。OgLuc変異体ポリペプチドは、野生型オキヒオドシエビ属(Oplophorus)ルシフェラーゼの対応するポリペプチドと比較して、増強された発光、増強されたシグナル安定性、および増強されたタンパク質安定性のうち少なくとも1つを有する。 【選択図】なし |
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| 20 | オキヒオドシエビ属由来ルシフェラーゼ、新規セレンテラジン基質、及び使用法 | JP2017084390 | 2017-04-21 | JP2017176182A | 2017-10-05 | ビンコウスキー ブロック; エンセル ランス ピー; ホール メアリー; ロバース マシュー ビー; スレーター マイケル アール; ウッド キース ヴイ; ウッド モニカ ジー |
| 【課題】増強された発光を有する、改変されたルシフェラーゼポリペプチドをコードする単離ポリヌクレオチドおよび基質の提供。 【解決手段】特定のアミノ酸配列及び特定のアミノ酸配列に対するアミノ酸置換を有する、オキヒオドシエビ属由来ルシフェラーゼ(OgLuc)変異体単離ポリヌクレオチドであって、非オキヒオドシエビ属ルシフェラーゼと比較して増強された発光を有する、単離ポリヌクレオチド。前記発光ポリペプチドを含むキット。更に特定の構造を有するセレンテラジン誘導体又は前記誘導体の異性体でカルボニル部が水酸基である互変異体のエステル誘導体を含むキット。 【選択図】なし |
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