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Clinically active bovine growth hormone fraction

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专利汇可以提供Clinically active bovine growth hormone fraction专利检索,专利查询,专利分析的服务。并且A clinically active component (TBGH-d) of a tryptic digest of bovine growth hormone (BGH) found to be homogeneous by disc electrophoresis and sedimentation equilibrium and having substantially the same molecular weight and amino acid composition as BGH was separated into two fractions, A-I and AII, by dextran gel filtration in 50% acetic acid. The amino acid analysis of the A-I and A-II fractions accounted for all the amino acids in the TBGH-d. The biological activity of the A-II fraction was found to be about 10-30% of TBGH-d. This fraction, having a molecular weight of about 5000 and an amino acid content or chain length of 38 amino acids, appears to exhibit human growth hormone-like activity. This fraction also appears to have wider applicability than simply being useful for the acceleration of somatic growth in humans. For example, this fraction would appear to have utility in promoting increased function and growth in an impaired liver and in effecting tissue regeneration, such as the promotion of healing of stress ulcers. It would also appear that this fraction has utility in the stimulation of protein synthesis to increase immunological competence and in wound and fracture healing. Also, it would appear that this fraction has utility in non-human applications, e.g., for increasing milk production, for increasing wool production and for accelerating animal growth.,下面是Clinically active bovine growth hormone fraction专利的具体信息内容。

1. A COMPOSITION CONSISTING ESSENTIALLY OF THE POLYPEPTIDE OBTAINED FROM THE TRYPTIC DIGEST OF BOVINE GROWTH HORMONE BY DIETHYLAMINOETHYL-CELLULOSE CHROMATOGRAPHY TO PRODUCE THEREFORM A HOMOGENEOUS COMPONENT AS EVIDENCE BY DISCELECTROPHORESIS AND SEDIMENTATION EQUILIBEIUM, SAID COMPONENT THEN SUBJECTED TO DEXTRAAN GEL FILTRATION IN THE PRESENCE OF 50% ACETIC ACID TO PRODUCE SAID POLYPEPTIDE, SAID POLYPEPTIDE HAVING A MOLECULAR WEIGHT OF ABOUT 5,000 AND A CHAIN LENGTH OF ABOUT 3, AMINO ACIDS, THE AMINO ACID CONTENT OF SAID POLYPEPTIDE COMPRISING ABOUT 2.0 LYSNE, ABOUT 2.8 ARGININE, ABOUT 3.8 ASPARTIC ACID AND ASPARAGINE, ABOUT 2.6 THREONINE, ABOUT 1.9 SERINE, ABOUT 4.9 GLUTAMIC ACID AND/OR GLUTAMINE, ABOUT 0.9 PROLINE, ABOUT 2.9 GLYCINE, ABOUT 1.0 ALANINE, ABOUT 3.0 VALINE, ABOUT 1.0 METHIONINE, ABOUT 0.9 ISOLEUCINE, ABOUT 5.1 LEUCINE, ABOUT 1.0 TYROSINE, ABOUT 1.8 PHENYLALANINE, HAVING VALINE AS THE AMINO TEWMINAL AMINO ACID AND ARGININE AS THE CARBOXY TERMINAL AMINO ACID, AND A CARRIER, SAID POLYPEPTIDE PRESENT IN SAID COMPOSITION AT A CONCENTRATION OF ABOUT 5 MILLIGRAMS PER MILLILITER.
2. A method of treating pituitary dwarfism in a human which comprises intramuscularly administering to the human the polypeptide of claim 1 in the amount of about 10 milligrams per day.
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