Method for the manufacture of a concentrate of a bifidogen factor |
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| 申请号 | US3556801D | 申请日 | 1967-01-18 | 公开(公告)号 | US3556801A | 公开(公告)日 | 1971-01-19 |
| 申请人 | URSINA AG; | 发明人 | HOSTETTLER HERMANN GUSTAV; | ||||
| 摘要 | THIS INVENTION RELATES TO A METHOD FOR THE MANUFACTURE OF A BIFIDOGEN FACTOR CALLED "BIFIDUS 2" (BF2) WITH ENHANCED ACTIVITY. THE PROCESS OF THE INVENTION IS CHARACTERIZED BY PASSING A PROTEIN HYDROLYSATE CONTAINING THE FACTOR BF2 AT LEAST TWOCE THROUGH A CHROMATOGRAPHIC COLUMN OF AN ION-EXCHANGE RESIN OR THROUGH A DEXTRAN GEL. THE FIRST CHROMATOGRAPHY IS CHARACTERIZED BY PASSING THE HYDROLYSATE THROUGH A COLUMN OF A STRONG ACID CATION EXCHANGE RESIN IN THE ACID FORM. THE SECOND CHROMATOGRAPHY IS CHARACTERIZED BY PASSING THE CONCENTRATED ELUATE FROM THE FIRST CHROMATOGRAPHY PROCESS THROUGH A COLUMN OF A WEAK ACID CATION EXCHANGE RESIN. THE BF2 CONCENTRATE IS THEN OBTAINED BY A FURTHER CHROMATOGRAPHIC PROCESS USING A BASIC ANION EXCHANGE RESIN, OR FILTERING THE SOLUTION THROUGH GEL. THE PROCESS IS DIRECTED TOWARDS OBTAINING AN IMPROVEMENT IN THE YIELD OF BF2 CONCENTRATE FROM PROTEIN HYDROLYSATE SOLUTION. | ||||||
| 权利要求 | |||||||
| 说明书全文 | |||||||
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