METHOD OF REUSING DNA-IMMOBILIZATION SUBSTRATE

申请号 EP01272814 申请日 2001-12-17 公开(公告)号 EP1347297A4 公开(公告)日 2007-06-06
申请人 TOYO KOHAN CO LTD; NIHON PARKERIZING HIROSHIMA CO; TAKAHASHI KOJIRO; 发明人 TAKAHASHI KOJIRO; TAKAI OSAMU; TANGA MICHIFUMI;
摘要 It is intended to provide a method of reusing a DNA-immobilization substrate whereby the expensive DNA-immobilization substrate can be efficiently utilized and a reusable DNA-immobilization substrate having the same performance as a new product without any trouble in practical use can be provided. Namely, a method of reusing a DNA-immobilization substrate characterized in that, to remove DNA from a DNA-immobilization substrate carrying the DNA immobilized by an acid-amide bond via an oligonucleotide to thereby enable the immobilization of a fresh DNA, the acid-amide bond between the substrate and the DNA is hydrolyzed with an acid or an alkali.
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