Strategies for high throughput identification and detection of polymorphisms |
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| 申请号 | US15729328 | 申请日 | 2017-10-10 | 公开(公告)号 | US09898577B2 | 公开(公告)日 | 2018-02-20 |
| 申请人 | Keygene N.V.; | 发明人 | Michael Josephus Theresia Van Eijk; Henricus Johannes Adam Van Der Poel; | ||||
| 摘要 | The invention relates to a method for identifying one or more polymorphisms in nucleic acid samples, comprising: (a) performing a reproducible complexity reduction on a plurality of nucleic acid samples to provide a plurality of libraries of the nucleic acid samples comprising amplified fragments, wherein the reproducible complexity reduction comprises amplifying fragments of the nucleic acid samples using one or more primers to obtain the amplified fragments, and wherein the amplified fragments in each library comprise a unique identifier sequence to indicate origin of each library obtained by the reproducible complexity reduction; (b) combining the plurality of libraries to obtain a combined library and sequencing at least a portion of the combined library to obtain sequences; (c) aligning the sequences to obtain an alignment; and (d) identifying one or more polymorphisms in the plurality of nucleic acid samples. | ||||||
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