SYSTEM FOR CONTINUOUS MUTAGENESIS IN VIVO TO FACILITATE DIRECTED EVOLUTION |
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申请号 | US15747282 | 申请日 | 2016-07-25 | 公开(公告)号 | US20180216097A1 | 公开(公告)日 | 2018-08-02 |
申请人 | Manel CAMPS; | 发明人 | Manel CAMPS; | ||||
摘要 | A system for continuous mutagenesis to facilitate directed evolution, the system including DNA polymerases carrying the novel K54E point mutation, and other point mutations including I709N, A759R, D424A (herein called K54E_LF Pol I) and this methods of use to produce and detect lines where mutagenesis is continuous and does not exhibit the usual decline in mutagenesis with sequential cloning. | ||||||
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说明书全文 |