SYSTEM FOR CONTINUOUS MUTAGENESIS IN VIVO TO FACILITATE DIRECTED EVOLUTION

申请号 US15747282 申请日 2016-07-25 公开(公告)号 US20180216097A1 公开(公告)日 2018-08-02
申请人 Manel CAMPS; 发明人 Manel CAMPS;
摘要 A system for continuous mutagenesis to facilitate directed evolution, the system including DNA polymerases carrying the novel K54E point mutation, and other point mutations including I709N, A759R, D424A (herein called K54E_LF Pol I) and this methods of use to produce and detect lines where mutagenesis is continuous and does not exhibit the usual decline in mutagenesis with sequential cloning.
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