CULTURE METHOD AND CULTURE DEVICE |
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| 申请号 | EP11823655 | 申请日 | 2011-09-08 | 公开(公告)号 | EP2615162A4 | 公开(公告)日 | 2015-04-29 |
| 申请人 | UNIV TSUKUBA; | 发明人 | FUKUDA JUNJI; SUZUKI HIROAKI; MOCHIZUKI NAOTO; KAKEGAWA TAKAHIRO; | ||||
| 摘要 | Provided are a culture method and a culture device for efficiently producing cells and/or cell tissue suitable for medication applications. A culture method of culturing cells adhered onto an electrode layer (30), the method comprising: a first step of adhering the cells onto the electrode layer and culturing the cells thereon, the electrode layer (30) being coated with an oligopeptide (10) including: a terminal amino acid (11) forming one end thereof to be bound to the electrode layer via thiolate; a cell adhesive sequence (14) forming another end thereof and having a cell-adhesive amino acid sequence; and an alternating sequence (13) to be bound to the one end side of the cell-adhesive sequence (14), the alternating sequence including a plurality of acidic amino acids (13a) and a plurality of basic amino acids (13b), being alternately bound to each other one by one; and a second step of applying, to the electrode layer (30) having the cells adhered thereonto, an electrical potential inducing reductive desorption of the oligopeptide (10), to thereby detach the cells from the electrode layer (30). | ||||||
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