| 序号 | 专利名 | 申请号 | 申请日 | 公开（公告）号 | 公开（公告）日 | 发明人 |
|---|---|---|---|---|---|---|
| 561 | Chiral crown ethers | US95012 | 1987-09-10 | US5047563A | 1991-09-10 | James B. Denton; Kin-Fai Yip |
| The synthesis and use of optically active isomers of bis(12-crown-4-methyl) dialkylmalonates as sodium specific ionophores is described. The synthesis of optically active isomers of hydroxymethyl 12-crown-4 useful in synthesizing ionophores is also described. | ||||||
| 562 | Blood gas monitoring sensors | US314615 | 1989-02-23 | US5047208A | 1991-09-10 | Jeffrey A. Schweitzer; Keith J. Proctor |
| Colorimetric, fiber optic sensors for measuring pH, PCO.sub.2 and/or other chemical parameters of the blood. The sensors are fabricated using a single optical fiber, which is provided with a chamber at its distal end containing a pH sensitive dye. Located distal to the chamber is a white reflective surface located within 0.04" from the end of the optical fiber, which enhances the performance of the sensor. | ||||||
| 563 | Determining the concentration of water soluble species in biological fluid | US408685 | 1989-09-18 | US5001067A | 1991-03-19 | Robert L. Coleman; Chung C. Young |
| Correcting an initial measurement of the concentration of a first water soluble species dissolved in a water-based component of a biological fluid that also includes a second water soluble species and a volume occupying component, the initial measurement having been taken using an original sample of the fluid that had been diluted by an amount of additional aqueous solution to form a diluted sample is disclosed; the concentration of the second water soluble species is measured in an undiluted sample of the biological fluid to obtain a direct concentration and is measured using a diluted sample of the fluid to obtain an indirect concentration, and the initial measurement of concentration of the first species is adjusted based on a combination of the direct and indirect concentration determinations of the second species. | ||||||
| 564 | Chromogenic cryptand reagents and methods for determining cation concentrations in test samples containing interfering cations | US038741 | 1987-04-15 | US4994395A | 1991-02-19 | Eddy Chapoteau; Bronislaw P. Czech; Carl R. Gebauer; Koon-Wah Leong; Anand Kumar |
| The invention is reagents and procedures for determining an amount of cations present in a sample, the reagent comprising(a) one or more chromogenic cryptand compounds of formula I ##STR1## wherein k and j, either same or different, are equal to 1 to about 5;m and n, either same or different, are equal to 0 to about 4;a and e, either same or different, are equal to 0 to about 2;b and d, either same or different, are equal to 0 to about 5;R, either same or different, is hydrogen, lower alkyl, lower alkylidene, lower alkenyl, allyl, or aryl; and--Q-- is ##STR2## wherein X is CH, N, or COH; andY includes ##STR3## and (b) one or more interfering cation complexing compound masks. | ||||||
| 565 | Xanthene dyes having a fused (C) benzo ring | US83459 | 1987-08-10 | US4945171A | 1990-07-31 | Richard P. Haugland; James Whitaker |
| Synthesis and applications of fluorescent dyes which are derivatives of benzo[c]xanthenes is described. The dyes exhibit pH dependent absorption and fluorescence spectra with pKas near the normal physiological range. Unlike fluorescein, the dyes exhibit emission of different characteristic wavelengths dependent on the pH of the medium. This permits several methods of measuring the pH of the medium in contact with the indicator including measuring two emissions with one excitation, selectively exiting the acid and base forms independently and measuring their emission at either single or dual wavelengths, or measuring the characteristic pH dependent absorption or fluorescence excitation spectral. Methods are presented for making the indicators permeant to cell membranes for the measurement of intracellular pH. | ||||||
| 566 | [2,2,1]Cryptand compounds that selectively bind sodium | US932643 | 1986-11-19 | US4843158A | 1989-06-27 | Gerald A. Smith |
| A compound having the property of selectively binding sodium ions in the presence of potassium ions and having the formula: ##STR1## where X is ##STR2## and where any aromatic ring may be substituted and/or may form part of a fused aromatic ring system.At least one of the aromatic rings may be substituted by a spectroscopic reporter group, a group which permits the compound to enter and remain in a cell and/or an electron donating or withdrawing group.A method of determining cytoplasmic sodium concentration by using this compound as a probe is also described. | ||||||
| 567 | Method and reagent for the quantitative determination of phosphorous in serum and urine | US220772 | 1988-07-18 | US4826773A | 1989-05-02 | Richard A. Kaufman; Henry J. Rosenfeld; Denise Zaunczkowski |
| A reagent for determining quantitatively the amount of phosphorous in bodily fluids which comprises a surface active agent, a strong acid, ammonium molybdate, a heavy metal chelating compound, and anti-oxidant, and deionized water is described. A method of using such a reagent is also described. | ||||||
| 568 | Compounds, reagents and procedures for determining cations | US38843 | 1987-04-15 | US4808539A | 1989-02-28 | Eddy Chapoteau; Bronislaw P. Czech; Carl R. Gebauer; Koon-Wah Leong; Anand Kumar |
| The invention is reagents and procedures for determining an amount of cations present in a sample, the reagent comprising one or more chromogenic cryptand compounds of formula I ##STR1## wherein k and j, either same or different, are equal to 1 to about 5;m and n, either same or different, are equal to 0 to about 4;a and e, either same or different, are equal to 0 to about 2;b and d, either same or different, are equal to 0 to about 5;R, either same or different, is hydrogen, lower alkyl, lower alkylidene, lower alkenyl, allyl, or aryl; and-Q- is ##STR2## wherein X is CH, N, or COH; andY includes ##STR3## except that when Q is ##STR4## wherein Y is selected from the group consisting of p-nitrophenylazo, 3-phenylisothiazalyl -5-azo, isothiazolyl-5-azo, thiazolyl-5-azo, 2,4,6-trinitrophenylazo, p-nitrostyryl, p-benzoquinonemonoimino and bis-(p-dimethylaminophenyl)hydroxymethyl, then the following condition cannot be present: that simultaneously b is equal to 0 or 1, d is equal to 0 or 1, j is equal to 1, n is equal to 1 or 2, a is equal to 1, e is equal to 1, k is equal to 1 and m is equal to 2. | ||||||
| 569 | Chemiluminescence method for assaying compounds containing primary amino groups using 1-cyano-2-substituted benz(f)- or naphth(f)-isoindole fluorescers | US837671 | 1986-03-10 | US4758520A | 1988-07-19 | Bodgan Matuszewski; Richard S. Givens; Robert G. Carlson; Takeru Higuchi; Takao Kawasaki; Osborne S. Wong |
| A chemiluminescence method for assaying compounds containing primary amino groups using 1-cyano-2-substituted benz(f)- or naphth(f)-isoindole fluorescers is disclosed. The 1-cyano-2-substituted benz(f)- or naphth(f)-isoindole (CBI or CNI) is formed by reacting a compound containing a primary amino group with naphthalene-2-3-dicarboxaldehyde or anthracene-2,3-dicarboxaldehyde in the presence of cyanide ion. The reaction product of hydrogen peroxide with an oxalate ester is then combined with the CBI or CNI to form an analyte which exhibits chemiluminescence. A detector is used to measure the chemiluminescence emission from the chemiluminescence derivatized analytes. Compounds containing primary amino groups which may be assayed according to the present invention include primary amines, amino acids, peptides and catecholamines. The method can also be adapted to the analysis of trace amounts of cyanide ion and hydrogen peroxide. | ||||||
| 570 | Analytical element and method for determination of magnesium ions | US857219 | 1986-04-29 | US4753890A | 1988-06-28 | Margaret J. Smith-Lewis; John C. Mauck; John L. Toner |
| An analytical element is used to determine magnesium ions at a pH of from about 8.5 to about 11. This element comprises an absorbent carrier material containing a 1,5-bis(2-hydroxyphenyl)-3-cyanoformazan compound which is substituted in at least one of the 3-, 4- and 5-positions of either phenyl moiety. The substituents are chosen such that their cumulative Hammett-sigma value is greater than about 0.23, provided that none of the substituents is carboxy. The assay is carried out in the presence of a calcium ion chelating agent and a suitable buffer. In preferred embodiments, the cyanoformazan is incorporated in the element in such a manner so as to be isolated from protein molecules which may be encountered in the assay. | ||||||
| 571 | Ion specific test method | US493969 | 1983-05-12 | US4734375A | 1988-03-29 | Steven C. Charlton |
| A test means for detecting the presence of a specific ion in an aqueous test sample and for determining its concentration comprises a substantially nonpolar, nonporous carrier matrix which is incorporated with an ionophore capable of selectively forming a complex with the ion under analysis. In addition, a counterion reporter substance is provided separately, which is capable of producing a detectable response, such as a change in or appearance of color or fluorescence in the carrier matrix. In use, the test sample containing the counterion reporter substance is contacted with the test means, the test means is washed and any detectable response associated with the test means is determined. | ||||||
| 572 | Rate method for the determination of inorganic phosphorus | US824689 | 1984-07-17 | US4731331A | 1988-03-15 | Frank R. Shu; James H. Francis |
| A reagent of the type comprising an acid, a molybdate salt, a non-ionic surfactant, and polyvinylpyrrolidone (PVP). The reagent is characterized in that it further comprises an enhancing amount of at least one sugar alcohol. Also, a method for assaying inorganic phosphorus of the type which comprises mixing a sample of biological fluid with a reagent and measuring the absorbance of the resulting solution. The method is characterized in that the above reagent is employed therein. The method can further be characterized in that the measurement can be made via a rate technique. | ||||||
| 573 | Method of treating diseases arising from platelet hyperactivation | US619212 | 1984-06-11 | US4728660A | 1988-03-01 | Duncan H. Haynes; Wenche Jy; Yeon S. Ahn; William J. Harrington |
| Calcium (Ca.sup.2+) channel blockers, such as nifedipine and verapamil, are used in the treatment of thromboembolic diseases such as stroke and peripheral vascular occlusive diseases, especially arterial and venous thrombosis, vasculitis, myelofibrosis disease and hemolytic anemias. Such diseases arise from platelet hyperactivation and the Ca.sup.2+ channel blockers restore the platelets to their normal aggregation characteristics. Diagnostic procedures for detecting platelet hyperactivation and defective calcium handling/transport indicative of certain peripheral obstructive diseases using chlorotetracycline as a detectable fluorescent probe as a means of assessing a patient's response to Ca.sup.2+ channel blockers in the therapy of such diseases are also described. | ||||||
| 574 | Method for determining intracellular mineral levels | US878936 | 1986-06-26 | US4717826A | 1988-01-05 | Burton B. Silver |
| Intracellular mineral concentrations are obtained from very small tissue samples, such as sublingual tissue smears. The tissue smears are subjected to excitation radiation, typically a scanning electron beam, and mineral analyses may be based on the profile of emitted x-ray fluorescence. A number of correlations are made between various diseased states, susceptibility to diseased states, metabolic status based on the elevated and depressed levels of some or all of the mineral concentrations. | ||||||
| 575 | Breast cyst fluid screening method for cancer risk assessment | US635846 | 1984-07-30 | US4695471A | 1987-09-22 | Martin Fleisher; H. Leon Bradlow; Morton K. Schwartz |
| A method for screening breast cyst fluid to identify patients having a high risk of developing breast cancer, comprising the steps of measuring the chloride ion content of breast cyst fluid aspirated from the patient; and correlating the chloride ion concentration with the risk of developing breast cancer. | ||||||
| 576 | Process for determining the pH value in the interior of a cell | US680078 | 1984-12-10 | US4677060A | 1987-06-30 | Gunter Valet; Gerhard Ruhenstroth-Bauer; Erich Wunsch; Luis Moroder |
| A process for determining the pH value in the interior of a cell is descrd. This is accomplished by measurement of the emission of a fluorescent substance which is absorbed by the cell through incubation in a solution. The solution contains a compound of the said fluorescent substance; the fluorescent substance is separated from this compound in the cell by an enzyme present in the cell. The fluorescent substance selected is one such that the frequency of the emission maximum is dependent on the pH value. The compound of this fluorescent substance which is selected is one which is membrane penetrating. Measurement of the fluorescence is based on the principle of measurement of the frequency at which the emission maximum occurs. A fluorescent substance suitable for this purpose is 2,3-dicyano-1,4-hydroquinone. A suitable membrane penetrating compound is 1,4-diacetoxy-2,3-dicyanobenzene or 1,4-dibutyryloxy-2,3-dicyanobenzene or 1,4-di(-tert-butyloxycarbonyl-1-alanyloxy)-2,3-dicyanobenzene. | ||||||
| 577 | Stable chloride test device | US697511 | 1985-02-01 | US4650768A | 1987-03-17 | Sally E. Cahill; Melvin D. Smith |
| A stable test device capable of manufacture by automated methods for the determination of chloride in an aqueous fluid sample. The test device operates by controlled diffusion of the aqueous sample into a porous matrix. The matrix is incorporated with sodium carrageenan and silver salts capable of dissolving in an aqueous fluid containing chloride, at least part of which silver salts is silver chromate. Devices prepared with the inclusion of sodium carrageenan provide a chloride test device with approximately double the shelf life under room temperature storage conditions of previous test devices prepared without sodium carrageenan. The stable test device provides useful information in less than about 2 minutes after the sample contacts the matrix. The chloride concentration of the sample is determined by comparing the pattern of silver chromate remaining after sample contact to an appropriate pattern chart. | ||||||
| 578 | Compound useful in detecting ions and method of preparing it | US493951 | 1983-05-12 | US4552697A | 1985-11-12 | Kin F. Yip; Steven C. Charlton |
| A novel compound is disclosed having the structure ##STR1## in which R is H or lower alkyl, R* is intermediate alkyl, and X is halogen or pseudohalogen. Also disclosed is a method for preparing the compound, the method including the steps of reacting (intermediate alkyl)benzene (I) with succinic anhydride to form (intermediate alkyl)-benzoylpropionic acid (II), successively reducing and dehydrating (II) to form 7-(intermediate alkyl)-1-tetralone (III), alkylating (III) to form 2-hydroxy(lower alkylidene)-7-(intermediate alkyl)-1-tetralone (IV), acrylating (IV) to form 2-acyloxy(lower alkylidene)-7-(intermediate alkyl)-1-tetralone (V), reacting (V) in the presence of an cycloalkene to form 7(intermediate alkyl)-2-(lower alkyl)-1-naphthol (VI), reacting (VI) with a 2,6-dihaloquinone-4-haloimide to form the compound (I), and isolating it. | ||||||
| 579 | Reagent mixture for measuring magnesium | US424180 | 1982-09-27 | US4503156A | 1985-03-05 | Fujio Yamazato; Kuniaki Tokuda; Toshihiko Oda |
| A reagent mixture for measuring magnesium comprising Xylylazo Violet I or II as a color producing agent and at least one of sulfide compounds and thioureide compounds, and if necessary one or more surface active agents, can be stored for a long period of time stably and can give a stable color development. | ||||||
| 580 | Process for determining the pH value in the interior of a cell | US346094 | 1982-02-05 | US4503148A | 1985-03-05 | G/u/ nter Valet; Gerhard Ruhenstroth-Bauer; Erich W/u/ nsch; Luis Moroder |
| A process for determining the pH value in the interior of a cell is described. This is accomplished by measurement of the emission of a fluorescent substance which is absorbed by the cell through incubation in a solution. The solution contains a compound of the said fluorescent substance; the fluorescent substance is separated from this compound in the cell by an enzyme present in the cell. The fluorescent substance selected is one such that the frequency of the emission maximum is dependent on the pH value. The compound of this fluorescent substance which is selected is one which is membrane penetrating. Measurement of the fluorescence is based on the principle of measurement of the frequency at which the emission maximum occurs. A fluorescent substance suitable for this purpose is 2,3-dicyano-1,4-hydroquinone. A suitable membrane penetrating compound is 1,4-diacetoxy-2,3-dicyanobenzene or 1,4-dibutyryloxy-2,3-dicyanobenzene or 1,4-di(-tert-butyloxycarbonyl-1-alanyloxy)-2,3-dicyanobenzene. | ||||||
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