| 序号 | 专利名 | 申请号 | 申请日 | 公开（公告）号 | 公开（公告）日 | 发明人 |
|---|---|---|---|---|---|---|
| 61 | TRANSGENIC ORGANISM | US12014057 | 2008-01-14 | US20090007284A1 | 2009-01-01 | Philippa Radcliffe; Kyriacos Mitrophanous; Michael Themis |
| A method of producing a transgenic cell comprising introducing into a cell a non-primate lentiviral expression vector comprising a nucleotide of interest (NOI). Also described is a method of producing a transgenic cell comprising introducing into a cell a lentiviral expression vector comprising a NOI capable of generating an antisense oligonucleotide, a ribozyme, an siRNA, a short hairpin RNA, a micro-RNA or a group 1 intron. Also described is a viral vector comprising a first nucleotide sequence, wherein said first nucleotide sequence comprises: (a) a second nucleotide sequence comprising an aptazyme; and (b) a third nucleotide sequence capable of generating a polynucleotide; wherein (a) and (b) are operably linked and wherein the aptazyme is activatable to cleave a transcript of the first nucleotide sequence such that said polynucleotide is generated. | ||||||
| 62 | Transgenic organism | US10421947 | 2003-04-24 | US20040040052A1 | 2004-02-26 | Philippa Radcliffe; Kyriacos Mitrophanous; Michael Themis |
| A method of producing a transgenic cell comprising introducing into a cell a non-primate lentiviral expression vector comprising a nucleotide of interest (NOI). Also described is a method of producing a transgenic cell comprising introducing into a cell a lentiviral expression vector comprising a NOI capable of generating an antisense oligonucleotide, a ribozyme, an siRNA, a short hairpin RNA, a micro-RNA or a group 1 intron. Also described is a viral vector comprising a first nucleotide sequence, wherein said first nucleotide sequence comprises: (a) a second nucleotide sequence comprising an aptazyme; and (b) a third nucleotide sequence capable of generating a polynucleotide; wherein (a) and (b) are operably linked and wherein the aptazyme is activatable to cleave a transcript of the first nucleotide sequence such that said polynucleotide is generated. | ||||||
| 63 | 테트라사이클린 저항성 유전자를 이용한 테트라사이클린 계열 항생제 탐지용 바이오센서 균주 | KR1020130166224 | 2013-12-27 | KR1020150077237A | 2015-07-07 | 박우준; 홍혜림 |
| 본발명은서열번호 2의염기서열을갖는테트라사이클린프로모터 (), 오퍼레이터및 테트라사이클린억제자(); 및상기프로모터에작동가능하게연결된리포터유전자를포함하는테트라사이클린계열항생제탐지용재조합벡터, 그리고이를포함한대장균() 또는아시네박터올레이보란스 DR1(DR1, 기탁번호 KACC91414P) 바이오센서균주를제공한다. 본발명에의하면, 테트라사이클린계열항생제를특징적으로신속, 정확하고효율적으로탐지할수 있고, 특히오염된환경에서도안정적으로테트라사이클린계열항생제를탐지할수 있다. | ||||||
| 64 | 활성 물질을 스크리닝하기 위한 유전자 변형된 생물체를생성하는 방법 | KR1020057011453 | 2003-11-18 | KR101215854B1 | 2012-12-31 | 클레블베르트; 스타들러애냐; 죌너로제마리에; 레베러에케하르트; 니체알무트 |
| 본발명은, a) 생물체의유전자변형에의해하나이상의단백질또는단백질단편의이종성발현을유도하고, b) 변형된유전자발현패턴을분석하고, 상쇄적차별적으로조절된유전자를확인하고, c) 당해생물체의표현형을결정하는단계를포함하는, 활성물질스크리닝용유전자변형된생물체의생성방법에관한것이다. 또한, 본발명은상기방법에의해수득될수 있는유전자변형된생물체에관한것이다. | ||||||
| 65 | ＰｅｐＭｏＶ에 대한 내성이 증진된 고추의 형질전환체 및 그 제조방법 | KR1020090108166 | 2009-11-10 | KR1020110051539A | 2011-05-18 | 한지학; 정민; 신선희; 최순호; 류기현 |
| PURPOSE: A transformant plant with enhanced resistance to viral diseases due to PepMoV(pepper mottle virus) is provided to reduce virus damage and to reduce agricultural chemical use and labor. CONSTITUTION: A transgenic plant with enhanced PepMoV viral disease resistance is prepared by transforming a plant with a recombinant plant RNAi expression vector containing PepMoV-derived HC-Pro(helper component-proteinase) gene. The HC-Pro gene has a base sequence of sequence number 1. The recombinant RNAi expression vector is pK7GWIWG2(II)::HC-Pro vector. The plant is Capsicum annuum. | ||||||
| 66 | 형질전환된 생물체에서 전위인자 벡터에 의해 전위를유발시키는 방법 | KR1020047010672 | 2003-01-09 | KR100985678B1 | 2010-10-05 | 크래이그,로저; 사바키스,차라람보스; 그로스벨드,프랑크 |
| 본 발명은 형질전환된 자손을 발생시키고 전위를 유발시키는 방법으로서, (a) 전위인자의 하나 이상의 복사체를 그 게놈내에 포함하는 제 1의 성숙한 형질전환된 생물체를 발생시키는 단계; (b) 상기 전위인자와 동족인 트랜스포사제를 암호화하는 유전자의 하나 이상의 복사체 및/또는 상기 트랜스포사제를 암호화하는 유전자의 발현을 조절할 수 있는 엘리먼트를 그 게놈내에서 포함하는 제 2의 성숙한 형질전환된 생물체를 발생시키는 단계; (c) 상기 제 1의 성숙한 형질전환된 생물체를 상기 제 2의 성숙한 형질전환된 생물체와 교잡하여, (i) 상기 전위인자의 하나 이상의 복사체와 (ii) 상기 전위인자와 동족인 트랜스포사제를 암호화하는 유전자를 그의 하나 이상의 세포의 게놈내에서 포함하는 자손을 제공하는 단계로서, 상기 트랜스포사제를 암호화하는 유전자는 상기 트랜스포사제의 발현을 가능하게 하는 하나 이상의 조절 서열의 조절하에 있는 단계; 및 (d) 상기 자손내에서 상기 트랜스포사제를 암호화하는 유전자의 발현을 유도하여 상기 자손의 조직 또는 세포의 최소한 일부분내에서 상기 전위인자를 이동시키는 단계를 포함하는 방법을 개시한다. 이러한 방법을 이용하면, 전위인자의 이동이 예정된 배아 발달 단계에서 유도되고 단일 세포의 변이된 유전자가 차후의 세포 분열시에 복제됨으로써, 상기 전위된 유전자와 실질적으로 상동인 세포 그룹이 얻어질 수 있는 이점이 있다. | ||||||
| 67 | 저니코틴 형질전환 담배식물체 및 이의 제조방법 | KR1020070022526 | 2007-03-07 | KR1020080082124A | 2008-09-11 | 김영국; 김현순; 정혁; 노문철; 이현선; 이중구; 전재흥; 권석윤 |
| A method is provided to raise a low nicotine tobacco, which is highly valuable as a material for producing a high quality tobacco with low nicotine content, by inhibiting expression of a putrescine-N-methyltransferase(PMT) gene using RNA interference technique. A method for producing a low nicotine transgenic tobacco comprises a step of inhibiting expression of a PMT gene through RNA interference(RNAi) technique. A low nicotine transgenic tobacco is characterized in that a nicotine content is at least 50% decreased by inhibiting the expression of the PMT gene. Further, the low nicotine transgenic tobacco is Burley species. | ||||||
| 68 | 유전자변형농산물에 대한 유전자변형농산물 혼입률(%)의정량분석방법 | KR1020020045752 | 2002-08-02 | KR100794700B1 | 2008-01-14 | 박한오; 김현배; 이상주; 박수민 |
| 본 발명은 유전자변형농산물(GMO)에 대한 GMO 혼입률(%)을 분석하기 위한 새로운 유전자변형농산물 정량분석방법에 관한 것이다. 보다 상세하게는 유전자변형농산물에 대한 재조합 유전자 검출용 프라이머와 내재유전자 검출용 프라이머의 각 5′말단을 형광표지로 라벨링하여 중합효소연쇄반응을 수행한 후, 각각의 표준시료에 대한 증폭산물 양을 기준으로 표준정량곡선을 작성하고 표준정량곡선 대비 미지시료에 대한 증폭산물의 양을 환산하여 미지시료의 GMO 혼입률(%)을 산출하는 단계로 이루어지는 유전자변형농산물에 대한 GMO 혼입률(%)의 정량분석방법에 관한 것이다. 본 발명의 방법은 기존의 정량방법과는 달리 보다 정확한 유전자변형농산물의 GMO 혼입률(%)을 정량할 수 있고, 초기 설비투자의 비용이나 기기 유지비용에 있어서도 경제적으로 효율성이 높다. 유전자변형농산물, GMO, 비유전자변형농산물, Non-GMO, 혼입률 | ||||||
| 69 | Transgenic mollusk and its production | JP4844499 | 1999-02-25 | JP2000245452A | 2000-09-12 | MIKI KEIZABURO; MIWA JOJI; ISOWA NOZOMI |
| PROBLEM TO BE SOLVED: To obtain a transgenic mollusk transduced with a desired extrinsic gene except for a gene imparting a viral resistance, having a capability for producing a colored pearl by expressing the foreign gene and useful for the production of pearl oyster, etc. SOLUTION: This transgenic mollusk is obtained by transducing an extrinsic gene that is a desired green colored fluorescent light-generating protein gene except for a gene imparting a viral resistance and a gene associated with coloring of a tissue generating a fluorescent light, and expressing the extrinsic gene. Further, the transgenic mollusk is produced by microinjecting the desired extrinsic gene to be incorporated or a recombinant vector obtained by transducing nucleic acids including the foreign gene into a vector to gonads of a male and/or female of the mollusk, mating these male and female to produce a first generation, and then selecting individual bodies expressing the desired gene. | ||||||
| 70 | 아가레즈 발현 벡터, 이의 형질전환체, 이 형질전환체를 이용한 아가레즈 제조방법 | KR1020110005050 | 2011-01-18 | KR1020120083741A | 2012-07-26 | 홍순광; 지원재; 오양가테무진; 장용근 |
| PURPOSE: A method for preparing agarase using a transformant prepared by an agarase expression vector is provided to simply and effectively obtain agarase. CONSTITUTION: A beta-agarase expression vector is prepared by cloning SCO3487 gene derived from Streptomyces coelicolor A3(2) to a replicable vector to control transcription by ermE promoter. The vector is Puwl201PW cloning vector. A host of a transformant is Streptomyces lividans. The beta-agarase is prepared by culturing the Actinomyces transformant. | ||||||
| 71 | TRANSGENIC BIOLUMINESCENT PLANTS | PCT/US2007/002517 | 2007-01-31 | WO2007136432A1 | 2007-11-29 | HUDKINS, Bruce, Eric |
Transgenic plants, and a method for making the same, wherein genes encoding the enzyme luciferase and its corresponding substrate luciferin are incorporated into a native plant genome. Once transformed into plant cells, these genes may be regulated such that under certain endogenous or exogenous conditions, their expression in the mature plant results in bioluminescence. Different Iuciferin/luciferase complexes and/or mechanisms of regulation may be utilized for these transgenic plants, depending on a variety of factors such as plant species and the circumstances under which a bio luminescent reaction is desired. Photo transformation may be utilized to vary the wavelength of light emitted from the mature plant. |
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| 72 | Conversion of desthiobiotin to biotin | JP3377294 | 1994-03-03 | JPH07236493A | 1995-09-12 | IFUKU OUJI; KOGA NOBUYOSHI; HAJI SHINICHIRO |
| PURPOSE:To obtain biotin which is a vitamin participating in the enzymatic reaction for fat metabolism, etc., as a coenzyme by fermentation method in high efficiency by adding flavotoxin to an enzymatic conversion reaction liquid containing desthloblotin and carrying out the reaction of the components. CONSTITUTION:Biotin which is a vitamin participating in the enzymatic reaction for fat metabolism, etc., as a coenzyme is produced in high efficiency by culturing E.coli W3110 (ATCC1498) in a medium, collecting and washing the cells in logarithmic growth stage, extracting the DNA by phenol method, amplifying the flavotoxin gene by PCR method using two synthetic primers positioned at both sides of a translation zone of the flavotoxin gene, linking the gene to a vector to form a recombinant DNA, inserting the DNA into E.coli to obtain a recombinant E.coli, culturing the strain to obtain flavotoxin, adding the flavotoxin to an enzymatic conversion reaction system composed of an enzymatic conversion reaction liquid prepared from E.coli having bioB gene in a biotin operon and subjecting the desthiobiotin to enzymatic conversion reaction. | ||||||
| 73 | TRANSGENIC BIOLUMINESCENT PLANTS | US12704330 | 2010-02-11 | US20100313303A1 | 2010-12-09 | Bruce Eric Hudkins |
| Transgenic plants, and a method for making the same, wherein genes encoding the enzyme luciferase and its corresponding substrate luciferin are incorporated into a native plant genome. Once transformed into plant cells, these genes may be regulated such that under certain endogenous or exogenous conditions, their expression in the mature plant results in bioluminescence. Different luciferin/luciferase complexes and/or mechanisms of regulation may be utilized for these transgenic plants, depending on a variety of factors such as plant species and the circumstances under which a bioluminescent reaction is desired. Phototransformation may be utilized to vary the wavelength of light emitted from the mature plant. | ||||||
| 74 | 유전자변형 식물체 분석용 프라이머 세트, 프로브 및표준플라스미드 | KR1020030013955 | 2003-03-06 | KR1020040079053A | 2004-09-14 | 김영미; 노재균; 김태산; 박용환; 이데레사; 안일평; 강상호 |
| PURPOSE: Primer sets and probes for detection of GMO, and a standard plasmid for qualitative and quantitative detection of GMO are provided, thereby effectively carrying out qualitative and quantitative detection of GMO in agricultural products and foods. CONSTITUTION: The insect tolerant primer set for detection of GMO(genetically modified organism) plants comprises one or two primers selected from NLL-F primer of SEQ ID NO:1, NLL-R primer of SEQ ID NO:2, Cry3A-F primer of SEQ ID NO:6, Cry3A-R primer of SEQ ID NO:7 and Cry3A-F2 primer of SEQ ID NO:8. The Cyr3A-pb probe of SEQ ID NO:9 is used together with the insect tolerant primer set for detection of GMO plant. The UGP-pb probe of SEQ ID NO:14 is used together with the insect tolerant primer set for detection of GMO plant. The insect and virus tolerant primer set for detection of GMO plant such as a potato comprises one or two primers selected from NLYL-F primer of SEQ ID NO:3, NLYM-R primer of SEQ ID NO:4 and NLPS-R primer of SEQ ID NO:5. The primer set for detection of UDP-glucose phosphorylase gene of natural or GMO plants comprises one or two primers selected from UGP-F primer of SEQ ID NO:10, UGP-R primer of SEQ ID NO:11, UGP-F2 primer of SEQ ID NO:12 and UGP-R2 primer of SEQ ID NO:13. The standard plasmid pCryugp is used for qualitative and quantitative detection of GMO plants. | ||||||
| 75 | TRANSGENIC BIOLUMINESCENT PLANTS | PCT/US0321531 | 2003-07-10 | WO2004006656A3 | 2004-09-23 | HUDKINS BRUCE ERIC |
| Transgenic plants are created having incorporated into them a luciferase enzyme gene and a corresponding luciferin substrate gene. These genes are regulated such that for a certain amount of time after dark, these genes are expressed resulting in bioluminescence. Different luciferin/luciferase combinations may be utilized for these transgenic plants, depending on the desired wavelength and the plant species transfected. | ||||||
| 76 | TRANSGENIC BIOLUMINESCENT PLANTS | PCT/US2007002517 | 2007-01-31 | WO2007136432A8 | 2009-07-09 | HUDKINS BRUCE ERIC |
| Transgenic plants, and a method for making the same, wherein genes encoding the enzyme luciferase and its corresponding substrate luciferin are incorporated into a native plant genome. Once transformed into plant cells, these genes may be regulated such that under certain endogenous or exogenous conditions, their expression in the mature plant results in bioluminescence. Different Iuciferin/luciferase complexes and/or mechanisms of regulation may be utilized for these transgenic plants, depending on a variety of factors such as plant species and the circumstances under which a bio luminescent reaction is desired. Photo transformation may be utilized to vary the wavelength of light emitted from the mature plant. | ||||||
| 77 | TRANSDERMAL TULOBUTEROL DELIVERY | PCT/US2004037305 | 2004-11-05 | WO2005046600A3 | 2005-12-22 | LEBO DAVID B; LEE JUNY; LUISI VINCENT; RYOO JE PHIL; TOIGO OLIVER J III |
| A transdermal tulobuterol delivery system, preferably in the form of a single-layer, drug-in-adhesive matrix patch, is disclosed comprising a relatively low, (less than five weight percent) concentration of tulobuterol base dissolved in a skin adhesive composition containing at least one skin permeation enhancer. The transdermal delivery system of this invention provides controlled release of the active ingredient, includes a relatively low concentration of tulobuterol within the skin-contacting adhesive formulation of a transdermal patch, and provides acceptable sustained transdermal delivery of the dissolved tulobuterol, as well as acceptable tack and. peel adhesive properties for the delivery device. | ||||||
| 78 | Genetically Modified Biological Cells | US14738273 | 2015-06-12 | US20150353941A1 | 2015-12-10 | Theresa O'Keefe |
| The present invention is based, in part, on our discovery of a way to configure expression cassettes so that the expression of a selectable marker protein, which is critical for the growth or survival of a cell, also results in the expression of a protein of interest in a biological cell. Accordingly, in one aspect, the invention features a genetically modified cell (e.g., a bacterial cell) that includes a chromosomally integrated or cytoplasmic expression cassette that includes a first nucleic acid sequence encoding a protein of interest an a second nucleic acid sequence encoding a selectable marker protein. The regulatory sequence (e.g., the sequence encoding a functional promoter) that drives expression of the required selectable marker protein also drives expression of the protein of interest. For that reason, we may refer to their expression as being “linked” or “functionally coupled.” | ||||||
| 79 | TRANSGENIC BIOLUMINESCENT PLANTS | US14496758 | 2014-09-25 | US20150026844A1 | 2015-01-22 | Bruce Eric Hudkins |
| Transgenic plants, and a method for making the same, wherein genes encoding the enzyme luciferase and its corresponding substrate luciferin are incorporated into a native plant genome. Once transformed into plant cells, these genes may be regulated such that under certain endogenous or exogenous conditions, their expression in the mature plant results in bioluminescence. Different luciferin/luciferase complexes and/or mechanisms of regulation may be utilized for these transgenic plants, depending on a variety of factors such as plant species and the circumstances under which a biolmuniescent reaction is desired. Phototransformation may be utilized to vary the wavelength of light emitted from the mature plant. | ||||||
| 80 | TRANSGENIC BIOLUMINESCENT PLANTS | US13109623 | 2011-06-02 | US20110231962A1 | 2011-09-22 | Bruce Eric Hudkins |
| Transgenic plants, and a method for making the same, wherein genes encoding the enzyme luciferase and its corresponding substrate luciferin are incorporated into a native plant genome. Once transformed into plant cells, these genes may be regulated such that under certain endogenous or exogenous conditions, their expression in the mature plant results in bioluminescence. Different luciferin/luciferase complexes and/or mechanisms of regulation may be utilized for these transgenic plants, depending on a variety of factors such as plant species and the circumstances under which a biolmuniescent reaction is desired. Phototransformation may be utilized to vary the wavelength of light emitted from the mature plant. | ||||||
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