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序号 专利名 申请号 申请日 公开(公告)号 公开(公告)日 发明人
41 用于制备反式聚类异戊二烯的方法、载体、转基因生物、用于制造充气轮胎的方法及用于制造橡胶制品的方法 CN202280057891.7 2022-08-19 CN117897496A 2024-04-16 山口晴彦; 井之上宫城雪乃; 中山亨; 高桥征司
发明的目的在于提供一种以酶促方式制备分子量大于105的反式聚类异戊二烯(反式‑1,4‑聚异戊二烯)的方法。本发明涉及一种用于制备反式聚类异戊二烯的方法,所述方法包括在体外使能够在不与任何脂质膜结合时产生分子量为104以上的产物的反式异戊烯基转移酶(tPT)家族蛋白与脂质膜结合。
42 기능획득 돌연변이 인삼 모상근의 제조방법 KR1020020028182 2002-05-21 KR1020030090177A 2003-11-28 정화지; 인동수; 이수영; 최동욱; 최필선; 유장렬; 고석민
PURPOSE: A production of method transgenic Panax Ginseng is provided, thereby rapidly and easily obtaining a library of the total mutant genome of Panax Ginseng. CONSTITUTION: A production method of transgenic Panax Ginseng comprises the steps of: (A) constructing an activation tagging vector containing an enhancer and a selection marker; (B) inserting the activation tagging vector into A. rhizogenes; (C) transforming Panax Ginseng with A. rhizogenes containing the vector; and (D) culturing the A. rhizogenes treated Panax Ginseng in a selection medium to select a transgenic Panax Ginseng, wherein the enhancer is CaMV 35S.
43 난초류의 형질전환 방법 KR1019980019613 1998-05-29 KR100271136B1 2001-02-01 한경환; 양재모
PURPOSE: Provided is a genetically manipulated orchid, specifically the Cymbidium sp. by the particle-mediated transformation technology. In details, provided is a method of stimulating generation of the meristematic tissue continuously before or after the DNA coated particle projection to transform the protocorm-like body(PLB). The PLB has morphogenesis and differentiation. The method offers a broadly applicable methodology of genetic technique to improve the orchids. CONSTITUTION: The transformation method comprises the steps of: (i) culturing the first PLB in a liquid medium to induce the second PLB; (ii) placing PLB containing the first and second PLB on the surface of target to project microparticles; (iii) projecting microparticles with adding physical acceleration, wherein the microparticles transmit copies of foreign gene structures including one of interested foreign gene and selective marker gene; (iv) inducing projected PLB to make the proliferating PLB which is capable of bringing out shoots, wherein PLB is cultured in a medium containing a selection substance of the selective gene which provides resistance to select transformed PLB in which the interested gene can be expressed; and (v) obtaining clone transformed orchid from the above shoots.
44 Lifter for rotary kiln JP8160991 1991-03-20 JPH04292785A 1992-10-16 SUGIYAMA TAKAYUKI; ITO AKIO
PURPOSE: To reduce a thermal energy loss of a rotary kiln in a lifter for the kiln. CONSTITUTION: Since a round rod 16 for securing a reinforcing anchor 17 is disposed at a position separate from an end 15a of a refractory unit 15 at the side of an inner surface of a shell, heat transfer from the anchor 17 and the rod 16 to the shell is suppressed. Since nuts 18 to be mounted detachably to a mold at both ends of the rod 16 are provided, the anchor 17 can be disposed at a predetermined position without positional deviation by mounting the nut 18 at the mold at the time of molding. COPYRIGHT: (C)1992,JPO&Japio
45 Lifter for rotary kiln JP2208982 1982-02-16 JPS58140589A 1983-08-20 NISHIKAWA AKIRA
46 형질전환 조류를 이용한 단백질의 생산방법 KR1020100076439 2010-08-09 KR1020120014404A 2012-02-17 한재용; 최진원; 권세창; 최인영
PURPOSE: A method for producing protein is provided to produce recombinant human interleukin 1 receptor antagonist protein. CONSTITUTION: A method for producing protein is as follows. The method uses a recombination vector which peculiarly expresses recombinant human interleukin 1 receptor antagonist protein as oviduct. The recombination vector comprises an avalbumin promoter and recombinant human interleukin 1 receptor antagonist protein-coding nucleotide sequence that is automatically connected to the promoter.
47 유전자 변형 농작물의 검출 키트 KR1020030062772 2003-09-08 KR1020050025831A 2005-03-14 박병철; 김환묵; 박성구; 고창원; 이아영; 문제선
A diagnostic kit for detecting GMO is provided, which kit accurately diagnoses GMO(genetically modified organism) by detecting proteins introduced newly by using genetical engineering within a short period. The diagnostic kit for detecting GMO(genetically modified organism) comprises: a detecting strip immobilizing a porous thin layer-type paper (1) and an antigen layer (2) wherein EPSPS(5-enolpyruvylshikimate-3-phosphate synthase) is bound to at least one side of the paper; and a test vessel with a standard solution containing a conjugate of a monoclonal antibody against EPSPS and gold colloid, wherein the size of pores of the porous thin layer-type paper is 8 to 12 micrometer; and the antigen layer comprises a conjugate of EPSPS-bovine serum albumin.
48 전이 유전자성 생물체와 세포 및 그들의 제조 방법 KR1019900701886 1989-12-21 KR1019910700333A 1991-03-14 로오세실리아; 리차진
내용 없음.
49 조류 골수세포를 이용한 생식세포를 제조하는 방법 및 그 골수세포를 이용한 형질전환 조류 생산 방법 및 조류 생식선 카이메라 KR1020100017707 2010-02-26 KR1020110098212A 2011-09-01 이훈택; 허영태; 양지훈; 이성호; 엄상준
본 발명은 수컷 조류의 뼈로부터 골수세포를 분리하는 단계; 상기 분리된 골수세포를 배지에서 배양하는 단계 및/또는 상기 배지에서 배양된 골수세포를 수용체 정소에 이식하는 단계를 포함하는 골수세포로부터 분화전환 (trans-differentiated)된 웅성 생식 세포를 제조하는 방법 및 상기 제조된 웅성 생식세포를 이용한 형질전환된 조류의 생산방법에 관한 것이다 .
50 Production of chrge transfer divices JP15929975 1975-12-29 JPS5283075A 1977-07-11 WADA KOUDOU
PURPOSE:To produce resistive gates by selectively forming an Si layer through photo resist on a gate oxide film and filling a conductor film between adjoining poly Si layers.
51 TRANSGENIC ORGANISM PCT/GB2002/005901 2002-12-23 WO2003056022A2 2003-07-10 RADCLIFFE, Philippa; MITROPHANOUS, Kyriacos; THEMIS, Michael

A method of producing a transgenic cell comprising into a cell a non-primate lentiviral expression vector comprising a nucleotide of interest (NOI). Also described is a method of producing a transgenic cell comprising introducing into a cell a lentiviral expression vector comprising a NOI capable of generating an antisense oligonucleotide, a ribozyme, an siRNA, a short hairpin RNA, a micro-RNA, a micro-RNA or a group 1 intron. Also described is a viral vector comprising a first nucleotide sequence, wherein said first nucleotide sequence comprises: (a) a second nucleotide sequence comprising an aptazyme; and (b) a third nucleotide sequence capable of generating a polynucleotide; wherein (a) and (b) are operably linked and wherein the aptazyme is activatable to cleave a transcript of the first nucleotide sequence such that said polynucleotide is generated.

52 TRANSGENIC ORGANISM EP94913121.0 1994-04-07 EP0693128A1 1996-01-24 VILLAND, Per; KLECZKOWSKI, Leszek; OLSEN, Odd-Arne; POULSEN, Peter; OKKELS, Finn; MARCUSSEN, Jan
A transgenic organism is described which has an increased starch yield. The preferred embodiment concerns a plant or plant cell containing a recombinant DNA construct containing, in operational relationship to a plant promoter sequence or sequences enabling the expression of the gene by the plant or plant cell thereby to enhance the rate of production and/or yield of starch by the plant or the plant cell, a DNA sequence encoding an exogenous ADP glucose pyrophosphorylase enzyme (AGP) or a sub-unit thereof which retains the enzymatic activity of the AGP enzyme, characterised in that the said DNA sequence is the gene sequence, including non-critical allelic variations thereof, encoding the barley (Hordeum vulgare) endosperm AGP or an active sub-unit thereof, or a variant thereof having non-critical amino acid substitution(s) or deletion(s) at one or more points in the amino acid sequences defining the barley endosperm AGP or either of its sub-units.
53 Transgenic organism US10082122 2002-02-26 US20030121062A1 2003-06-26 Philippa Radcliffe; Kyriacos Mitrophanous; Michael Themis
A method of producing a transgenic cell comprising introducing into a cell a non-primate lentiviral expression vector comprising a nucleotide of interest (NOI).
54 저온저항성 박 대목 형질전환체 및 이의 제조 방법 KR1020140152013 2014-11-04 KR1020160053396A 2016-05-13 안율균; 김정호; 이혜은; 김도선; 박미희; 최근진
본발명은서열번호 1의염기서열로구성된애기장대유래의저온저항성증진유전자를포함하는재조합벡터를박 대목식물세포에형질전환시키는단계를포함하는박 대목형질전환체제조방법과이를이용한저온저항성형질전환박 대목에관한것이다.
55 무측지성 국화 형질전환체 및 이의 제조 방법 KR1020130140952 2013-11-19 KR1020150057512A 2015-05-28 이수영; 한봉희; 허은주; 이은경; 김원희; 권오현; 이혜진; 김정호; 천경성
본발명은서열번호 1의염기서열로구성된토마토유래의 lateral suppression(LeLS) 유전자를포함하는재조합벡터를국화식물세포에형질전환시키는단계를포함하는국화형질전환체제조방법과이를이용한형질전환된국화에관한것이다.
56 유전자 변형 식물의 스크리닝 방법 KR1020100045907 2010-05-17 KR101218435B1 2013-01-03 이명훈; 한상미; 이수빈; 이현철
본발명은유전자변형식물의스크리닝방법에관한것으로서, 보다상세하게는경쟁적 PCR 및질량분석방법을이용하여유전자변형식물을검출하는방법에관한것이다. 또한본 발명은유전자변형식물검출용프라이머세트및 경쟁적 PCR 수행을위한경쟁적서열, 및상기프라이머세트및 경쟁적서열을포함하는유전자변형식물검출용키트를제공한다.
57 생식세포-특이적 유전자 발현조절 서열을 이용한 형질전환 조류 생산 KR1020100115328 2010-11-19 KR1020120054119A 2012-05-30 한재용; 서희원; 이형철; 박태섭; 정진경
PURPOSE: The production of transgenic aves using sequences for germ cell-specific gene expression is provided to analyze the differentiation or development of the priordial germ cells of aves into germ cells. CONSTITUTION: A method for producing transgenic aves includes the following: the priordial germ cells of aves are transformed based on a genetic vector containing a DAZL promoter as a germ cell-specific promoter, a NANOG promoter as a stem cell-specific promoter, and nucleotide sequence which encodes protein of revelation purpose; the transformed priordial germ cells of the aves are injected into accept media; and the transformed product of the aves is obtained. The protein is operatively bonded to the promoters.
58 GENETICALLY MODIFIED ORGANISMS US14963836 2015-12-09 US20160168598A1 2016-06-16 JOHN ARTHUR LEIGH; THOMAS JOSEPH LIE
Provided herein is technology relating to genetically modified organisms and particularly, but not exclusively, to compositions comprising one or more genetically modified microorganisms, and related methods and systems, for producing liquid fuels from alkanes such as methane.
59 TRANSGENIC ORGANISM PCT/EP9401082 1994-04-07 WO9424292A3 1995-06-01 VILLAND PER; KLECZKOWSKI LESZEK; OLSEN ODD-ARNE; POULSEN PETER; OKKELS FINN; MARCUSSEN JAN
The present invention relates to transgenic plants or algae expressing an AGP enzyme coupled to a transit peptide. In particular, the present invention relates to transgenic plants or algae in which the activity of the AGP enzyme or subunit thereof is substantially independent of any level of in vivo 3-phospho-glycerate and any in vivo level of inorganic phosphate and wherein the activity of the AGP enzyme or subunit thereof is not stimulated by fructose-1,6-bisP and is not inhibited by AMP.
60 TRANSGENIC ORGANISM PCT/EP1994001082 1994-04-07 WO1994024292A2 1994-10-27 DANISCO A/S
A transgenic organism is described which has an increased starch yield. The preferred embodiment concerns a plant or plant cell containing a recombinant DNA construct containing, in operational relationship to a plant promoter sequence or sequences enabling the expression of the gene by the plant or plant cell thereby to enhance the rate of production and/or yield of starch by the plant or the plant cell, a DNA sequence encoding an exogenous ADP glucose pyrophosphorylase enzyme (AGP) or a sub-unit thereof which retains the enzymatic activity of the AGP enzyme, characterised in that the said DNA sequence is the gene sequence, including non-critical allelic variations thereof, encoding the barley (Hordeum vulgare) endosperm AGP or an active sub-unit thereof, or a variant thereof having non-critical amino acid substitution(s) or deletion(s) at one or more points in the amino acid sequences defining the barley endosperm AGP or either of its sub-units.
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