| 序号 | 专利名 | 申请号 | 申请日 | 公开（公告）号 | 公开（公告）日 | 发明人 |
|---|---|---|---|---|---|---|
| 181 | PRODUCTION OF BIOFILAMENTS IN TRANSGENIC ANIMALS | PCT/IB9900763 | 1999-03-12 | WO9947661A3 | 2000-01-06 | KARATZAS COSTAS N; TURNER JEFFREY D; KARATZAS ANTHOULA-LAZARIS |
| Disclosed is a method for the recombinant production of biofilaments, such as spider silk or insect fibroins, using transgenic animals which secrete the biofilaments in their milk and/or urine, and transgenic cells which secrete the biofilaments into culture media. Such a method is useful for producing large quantities of biofilament material. Also disclosed is a nucleic acid molecule for generating such transgenic animals. | ||||||
| 182 | PRODUCTION OF BIOFILAMENTS IN TRANSGENIC ANIMALS | PCT/IB1999/000763 | 1999-03-12 | WO99047661A2 | 1999-09-23 | |
| Disclosed is a method for the recombinant production of biofilaments, such as spider silk or insect fibroins, using transgenic animals which secrete the biofilaments in their milk and/or urine, and transgenic cells which secrete the biofilaments into culture media. Such a method is useful for producing large quantities of biofilament material. Also disclosed is a nucleic acid molecule for generating such transgenic animals. | ||||||
| 183 | ENGINEERING PROTEIN POSTTRANSLATIONAL MODIFICATION IN TRANSGENIC ORGANISMS | PCT/US9606121 | 1996-05-06 | WO9634966A3 | 1996-12-05 | LUBON HENRYK; DROHAN WILLIAM N; PALEYANDA REKHA K |
| The invention relates to transgenic non-human multicellular organisms that contain polynucleotides for expressing proteins that alter posttranslational modification. In particular, the invention provides multiply-transgenic animals in which a first transgene encodes a first protein, a second transgene encodes a second protein, and expression of the second protein affects the posttranslational modification of the first protein in cells of said organism. Expression in preferred embodiments is in specific cells and the modified protein is secreted into a bodily fluid. The invention provides related methods, proteins and products. An example provides transgenic animals that express human Protein C and the processing protease PACE/furin in mammary glands and secrete both proteins into milk. | ||||||
| 184 | ENGINEERING PROTEIN POSTTRANSLATIONAL MODIFICATION IN TRANSGENIC ORGANISMS | PCT/US1996006121 | 1996-05-06 | WO1996034966A2 | 1996-11-07 | AMERICAN RED CROSS; LUBON, Henryk; DROHAN, William, N.; PALEYANDA, Rekha, K. |
| The invention relates to transgenic non-human multicellular organisms that contain polynucleotides for expressing proteins that alter posttranslational modification. In particular, the invention provides multiply-transgenic animals in which a first transgene encodes a first protein, a second transgene encodes a second protein, and expression of the second protein affects the posttranslational modification of the first protein in cells of said organism. Expression in preferred embodiments is in specific cells and the modified protein is secreted into a bodily fluid. The invention provides related methods, proteins and products. An example provides transgenic animals that express human Protein C and the processing protease PACE/furin in mammary glands and secrete both proteins into milk. | ||||||
| 185 | TRANSGENIC BIRDS THAT PRODUCE CHIMERIC HUMAN IMMUNOGLOBULINS | US15142097 | 2016-04-29 | US20160309686A1 | 2016-10-27 | Philip A. Leighton; Emily J. Cadera |
| The invention relates to transgenic birds capable of producing chimeric immunoglobulins, with a combination of human and avian sequence, in their B cells. In some embodiments, the birds are chickens. When challenged with an antigen, the transgenic avians produce antigen-specific functional antibodies. The invention also relates to light chain immunoglobulin transgenes for making such transgenic avians, as well as methods and vectors for disrupting endogenous immunoglobulin loci in birds. | ||||||
| 186 | High throughput screening of genetically modified photosynthetic organisms | US12156432 | 2008-05-30 | US08268553B2 | 2012-09-18 | Stephen Mayfield; Bryan O'Neill; Michael Mendez; Kari Mikkelson; Yan Poon |
| The present invention provides a method and compositions for high throughput screening of genetically modified photosynthetic organisms for plasmic state. The present invention provides methods of producing one or more proteins, including biomass degrading enzymes in a plant. Also provided are the methods of producing biomass degradation pathways in alga cells, particularly in the chloroplast. Single enzymes or multiple enzymes may be produced by the methods disclosed. The methods disclosed herein allow for the production of biofuel, including ethanol. | ||||||
| 187 | Method of generating transgenic organisms using transposons | US12655114 | 2009-12-23 | US20110185442A1 | 2011-07-28 | Charalambos Savakis; Frank Grosveld |
| The invention relates to a method for generating a transgenic organism. The invention also relates to a method for detecting and characterizing a genetic mutation in a transgenic organism. The invention further relates to a method for isolating a gene which is correlated with a phenotypic characteristic in a transgenic animal. The invention further relates to a method for isolating an exon in a transgenic animal. The invention also relates to a method for modulating the expression of a gene in an organism. | ||||||
| 188 | Transgenic organisms with lower growth temperature | US10575505 | 2004-10-11 | US07811784B2 | 2010-10-12 | Manuel Ferrer; Kenneth Timmis; Tatjana Chernikova; Peter Golyshin; Michail Yakimov |
| The invention relates to the growth temperature of organisms, especially plants and microorganisms and the manipulation of the tolerable cultivation temperature. More specifically, the present invention relates to the expression of heterologous proteins in microorganisms, and especially to the heterologous expression of heat sensitive proteins in bacteria, either gram-negative or gram-positive. In a first aspect, the present invention provides a method for manipulation of cells and the resultant cells, wherein at least one gene from a psychrophilic micro organism coding for at least one chaperone or chaperonin is expressed. Such cells are selected among cultivated eukaryotic cells, i.e. animal and plant cells and entire plants, gram-negative and gram-positive bacteria, fungi and yeasts. | ||||||
| 189 | Process of producing environmentally safe transgenic organisms | US10512879 | 2002-04-29 | US07632981B2 | 2009-12-15 | Stefan Werner; Sylvestre Marillonnet; Victor Klimyuk; Yuri Gleba |
| A process of producing a transgenic multi-cellular plant or animal organism expressing a trait of interest and having a controlled distribution of said trait to progeny, wherein said process comprises hybridising a first multi-cellular organism or a cell thereof having a first heterologous DNA sequence comprising a first fragment of a nucleotide sequence encoding said trait of interest and a second multi-cellular organism or a cell thereof having a second heterologous DNA sequence comprising a second fragment of the nucleotide sequence encoding said trait of interest, whereby said first and said second heterologous sequences are designed such that said trait of interest arises due to RNA trans-splicing after said hybridation. | ||||||
| 190 | Transgenic organisms with lower growth temperature | US10575505 | 2004-10-11 | US20090255014A1 | 2009-10-08 | Manuel Ferrer; Kenneth Timmis; Tatjana Chernikova; Peter Golyshin; Michail Yakimov |
| The invention relates to the growth temperature of organisms, especially plants and microorganisms and the manipulation of the tolerable cultivation temperature. More specifically, the present invention relates to the expression of heterologous proteins in microorganisms, and especially to the heterologous expression of heat sensitive proteins in bacteria, either gram-negative or gram-positive. In a first aspect, the present invention provides a method for manipulation of cells and the resultant cells, wherein at least one gene from a psychrophilic micro organism coding for at least one chaperone or chaperonin is expressed. Such cells are selected among cultivated eukaryotic cells, i.e. animal and plant cells and entire plants, gram-negative and gram-positive bacteria, fungi and yeasts. | ||||||
| 191 | Method of generating transgenic organisms using transposons | US10245441 | 2002-09-17 | US20030150007A1 | 2003-08-07 | Charalambos Savakis; Frank Grosveld |
| The invention relates to a method for generating a transgenic organism. The invention also relates to a method for detecting and characterizing a genetic mutation in a transgenic organism. The invention further relates to a method for isolating a gene which is correlated with a phenotypic characteristic in a transgenic animal. The invention further relates to a method for isolating an exon in a transgenic animal. The invention also relates to a method for modulating the expression of a gene in an organism. | ||||||
| 192 | Genetically engineered organisms expressing surface proteins of T. cruzi | US537798 | 1983-09-30 | US4615973A | 1986-10-07 | Paul Lizardi; Nadia Nogueira |
| Genetically engineered plasmids which express DNA encoding for insect stage specific glycoproteins of Trypanosoma cruzi are disclosed. The glycoproteins offer potential diagnostic utility for the detection of Chagus disease. | ||||||
| 193 | METHOD FOR IMPROVING TURFGRASS ABIOTIC STRESS TOLERANCE | PCT/US2015/058288 | 2015-10-30 | WO2016073301A1 | 2016-05-12 | JAMES, John Robert; DANT, Lukas |
The present invention relates to a method of controlling abiotic stress on warm-season turfgrass using an effective amount of acibenzolar- s-methyl. |
||||||
| 194 | VERFAHREN ZUR HERSTELLUNG VON MEHRFACH UNGESÄTTIGTEN FETTSÄUREN IN TRANSGENEN ORGANISMEN | PCT/EP2008/052358 | 2008-02-27 | WO2008104559A1 | 2008-09-04 | ABBADI, Amine; FEUSSNER, Ivo; HOFFMANN, Mareike |
Die vorliegende Erfindung betrifft ein Verfahren zur Herstellung von mehrfach ungesättigten Fettsäuren, insbesondere langkettigen mehrfach ungesättigten Fettsäuren wie Arachidonsäure und/oder Eicosapentaensäure, in einem transgenen Organismus, indem Nukleinsäuren in den Organismus eingebracht werden, die für Polypeptide mit delta-6-Desaturase-, delta-6-Elongase- und/oder delta-5-Desaturase-Aktivität kodieren. Vorteilhaft stammen die delta-6-Desaturase und die delta-5-Desaturase aus Mantoniella squamata und die delta-6-Elongase aus Physcomitrella patens. Vorteilhaft wird in dem Organismus weiterhin ein Gen, das für eine omega-3-Desaturase kodiert, exprimiert. In einer weiteren vorteilhaften Ausführungsform des Verfahrens können weitere Nukleinsäuresequenzen, die für Polypeptide der Biosynthese des Fettsäure- und Lipidstoffwechsels kodieren, in dem Organismus exprimiert werden. Besonders vorteilhaft sind hierfür die Nukleinsäuresequenzen, die für eine delta-8-Desaturase-, delta-12-Desaturase-, delta-15-Desaturase, delta-4-Desaturase, delta-9-Elongase- und/oder delta-5-Elongase-Aktivität kodieren. |
||||||
| 195 | VERFAHREN ZUR HERSTELLUNG MEHRFACH UNGESÄTTIGTER FETTSÄUREN IN TRANSGENEN ORGANISMEN | PCT/EP2007/060554 | 2007-10-04 | WO2008040787A2 | 2008-04-10 | BAUER, Jörg; WETJEN, Tom |
Die vorliegende Erfindung betrifft Polynucleotide aus Ostreococcus lucimarinus, die Desaturasen und Elongasen kodieren und zur rekombinanten Herstellung von mehrfach ungesättigten Fettsäuren eingesetzt werden können. Weiterhin betrifft die Erfindung Vektoren, Wirtszellen und transgene nicht-humane Organismen, die die Polynucleotide enthalten, sowie die von den Polynucleotiden kodierten Polypeptide. Schließlich betrifft die Erfindung noch Herstellungsverfahren für die mehrfach ungesättigten Fettsäuren und für Öl-, Lipid- und Fettsäurezusammensetzungen. |
||||||
| 196 | VERFAHREN ZUR HERSTELLUNG MEHRFACH UNGESÄTTIGTER FETTSÄUREN IN TRANSGENEN ORGANISMEN | PCT/EP2005/013803 | 2005-12-21 | WO2006069710A1 | 2006-07-06 | CIRPUS, Petra; BAUER, Jörg; HEINZ, Ernst; DOMERGUE, Frederic |
Die vorliegende Erfindung betrifft ein Verfahren zur Herstellung von mehrfach ungesättigten Fettsäuren in einem Organismus, indem Nukleinsäuren in den Organismus eingebracht werden, die für Polypeptide mit ?-5-Elongase-, ?-6-Desaturase-, eine ?-5-Desaturase-, ?-4-Desaturase-, ?-12-Desaturase- und/oder ?-6-Elongaseaktivität codieren. Vorteilhaft stammen diese Desaturasen und Elongasen aus Ostreococcus. Weiterhin betrifft die Erfindung ein Verfahren zur Herstellung von Ölen und/oder Triacylglyceriden mit einem erhöhten Gehalt an langkettigen mehrfach ungesättigten Fettsäuren. Die Erfindung betrifft weiterhin die Nukleinsäuresequenzen, Nukleinsäurekonstrukte, Vektoren und Organismen enthaltend die erfindungsgemässen Nukleinsäuresequenzen, Vektoren enthaltend die Nukleinsäuresequenzen und/oder die Nukleinsäurekonstrukte sowie transgene Organismen enthalten die vorgenannten Nukleinsäuresequenzen, Nukleinsäurekonstrukte und/oder Vektoren. Ein weiterer Teil der Erfindung betrifft Öle, Lipide und/oder Fettsäuren hergestellt nach dem erfindungsgemässen Verfahren und deren Verwendung. Ausserdem betrifft die Erfindung ungesättigte Fettsäuren sowie Triglyceride mit einem erhöhten Gehalt an ungesättigten Fettsäuren und deren Verwendung. |
||||||
| 197 | TRANSGENIC ORGANISMS WITH LOWER GROWTH TEMPERATURE | PCT/EP2004052492 | 2004-10-11 | WO2005035750A2 | 2005-04-21 | FERRER MANUEL; TIMMIS KENNETH N; CHERNIKOVA TATJANA; GOLYSHIN PETER; YAKIMOV MICHAIL |
| The present invention in general relates to the growth temperature of organisms, especially plants and microorganisms and the manipulation of the tolerable cultivation temperature. More specifically, the present invention relates to the expression of heterologous proteins in microorganisms, and especially to the heterologous expression of heat sensitive proteins in bacteria, either gram-negative or gram-positive. In a first aspect, the present invention provides a method for manipulation of cells and the resultant cells, characterized in that at least one gene from a psychrophilic micro organism coding for at least one chaperone or chaperonin is expressed. Such cells are selected among cultivated eukaryotic cells, i.e. animal and plant cells and entire plants, gram-negative and gram-positive bacteria, fungi and yeasts. | ||||||
| 198 | PRODUCTION OF A TRANSGENIC AVIAN BY CYTOPLASMIC INJECTION | PCT/US2004009253 | 2004-03-26 | WO2004092351A2 | 2004-10-28 | RAPP JEFFREY; CHRISTMANN LEANDRO; HARVEY ALEX; LEAVITT MARKLEY |
| The invention provides methods for integrating a heterologous polynucleotide into the genome of an avian cell. The methods deliver to an avian cell a polynucleotide and a source of integrase activity that mediates recombination between the polynucleotide and the genomic DNA of the avian cell. The invention provides modified avian or artificial chromosomes as vectors to shuttle transgenes or gene clusters into an avian genome. Another aspect of the invention are avian cells genetically modified with a transgene vector. One cell line for the delivery and integration of a transgene comprises a heterologous attP site and, optionally, a region for expressing the integrase. Methods are also included for the production of a heterologous polypeptide by transgenic avian tissue involve integrating a heterologous polynucleotide into the avian genome. The present invention also relates to methods of producing transgenic chickens which include introducing into an avian cell a nucleic acid comprising a transgene and an integrase activity in addition to a cationic polymer and/or a nuclear localization signal and introducing the avian cell into a recipient avian wherein the recipient avian produces an offspring which includes the transgene. Also included are methods of dispersing a nucleic acid in a cell. | ||||||
| 199 | METHOD FOR PRODUCING AMINOACIDS | PCT/EP0314649 | 2003-12-19 | WO2004057003A3 | 2004-10-14 | SCHMITZ OLIVER; PUZIO PIOTR; BLAU ASTRID; LOOSER RALF; WENDEL BIRGIT; KAMLAGE BEATE; PLESCH GUNNAR |
| The invention relates to a method for producing aminoacids in transgenic organisms. The inventive method consists of the following steps: a) introduction of nucleic acids sequence which codes threonine decomposing protein or lysine decomposing protein or codes threonine decomposing protein and lysine decomposing protein, b) introduction of nucleic acids sequence which improves the decomposition of threonine or lysine or the decomposition of threonine and lysine in the transgenic organisms; c) expression of (a) or (b) nucleic acids sequence in a transgenic organism. In a very useful manner, the nucleic acids sequence is introduced in the step a) of the method, said sequence being selected from: i) the nucleic acids sequence with the sequence present in SEQ ID NO: 1, SEQ ID NO:11, SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO: 17, SEQ ID NO: 19, SEQ ID NO: 21, SEQ ID NO: 23 and/or SEQ ID NO:25; ii) the nucleic acids sequence which is preserved as a result of a degenerate genetic code by re-recording aminoacids sequence present in SEQ ID NO: 2, SEQ ID NO: 12, SEQ ID NO:14, SEQ ID NO: 16, SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:22, SEQ ID NO:24 and/or 26; and iii) a derivative of the nucleic acid sequence present in SEQ ID NO: 1, SEQ ID NO:11, SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO: 17, SEQ ID NO: 19, SEQ ID NO: 21, SEQ ID NO: 23 and/or SEQ ID NO:25 which codes polypeptides with the nucleic acids sequence present in SEQ ID NO: 2, SEQ ID NO: 12, SEQ ID NO:14, SEQ ID NO: 16, SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:22, SEQ ID NO:24 and/or 26 and which comprises at least 50 % of homology in terms of aminoacids without reducing the biological activity of polypeptides. | ||||||
| 200 | METHOD FOR PRODUCING KETOCAROTINOIDS IN GENETICALLY MODIFIED ORGANISMS | PCT/EP0309106 | 2003-08-18 | WO2004018694A3 | 2004-09-10 | SAUER MATT; FLACHMANN RALF; KLEBSATTEL MARTIN; SCHOPFER CHRISTEL RENATE |
| The invention relates to a method for producing ketocarotinoids by cultivating genetically modified organisms having a modified ketolase activity compared to the wild type, to genetically modified organisms, and to the use thereof as foodstuffs and fodder and for producing ketocarotinoid extracts. | ||||||
QQ群二维码
意见反馈
意见反馈