| 序号 | 专利名 | 申请号 | 申请日 | 公开（公告）号 | 公开（公告）日 | 发明人 |
|---|---|---|---|---|---|---|
| 181 | Biochemical new method for producing 7-adca | JP23973199 | 1999-08-26 | JP3072101B2 | 2000-07-31 | ジヨン・エイ・ランボセツク; フイリス・シー・マクエイダ; マイケル・ジエイ・コンダー; ロリリー・クラウフオード |
| 182 | Recovery method of cephalexin | JP52343696 | 1996-02-01 | JPH11503408A | 1999-03-26 | デン トィール，ウィルヘルムス，ヨハネス，ヨセフ ファン; ボエステン，ウィルヘルムス，フバルタス，ヨセフ; ルース，エリク，コルネリス |
| (57)【要約】 セファレキシンおよび７−アミノデスアセトキシセファロスポラン酸（７−ＡＤＣＡ）を含む混合物からのセファレキシンの回収方法であって、セファレキシンおよび７−ＡＤＣＡを含み、ｐＨが７より高く、存在しうる固体セファレキシンを別にしてｐＨ 7〜8.5 で均一である混合物に、7.8 より低いｐＨ値に達するまでｐＨ修正を施し、存在する固体物質を回収する方法。 その方法は特に、７−ＡＤＣＡとアシル化剤としてのフェニルグリシン誘導体との酵素アシル化反応の後に得られる反応混合物への適用に適する。 こうして、純粋なセファレキシンを簡単な方法で回収することができる。 | ||||||
| 183 | Cephamycin biosynthesis late enzyme coding dna | JP52773595 | 1995-04-17 | JPH09512170A | 1997-12-09 | エンジタ，フランシスコ・ホタ; コケ，フアン・ホセ・エレ; フウエンテ，フアン・エレ; マルテイン，フアン・エフエ; リヤレーナ，フランシスコ・ホタ; リラス，パロマ |
| (57)【要約】 抗生物質セファマイシンの合成に関与する後期酵素をコードするＤＮＡを単離精製した。 この特異的酵素はセファマイシン生合成の後期段階に関与する。 これらのＤＮＡを配列決定し、宿主細胞における組換え発現のために組換え発現ベクター中にクローニングした。 ＤＮＡ、このＤＮＡを含むベクター、及び、このベクターを発現させる組換え宿主細胞が、抗生物質の生産のために有用である。 | ||||||
| 184 | Cephalosporin-acetyl hydrolase gene and the protein encoded by the gene | JP9516691 | 1991-04-25 | JP2651288B2 | 1997-09-10 | MITSUSHIMA KENJI; TAKIMOTO AKIO; YAGI SHIGEO; SONOYAMA TAKAYASU |
| A cephalosporin acetylhydrolase gene, a recombinant DNA molecule prepared by introducing said gene into a vector used in an E. coli host-vector system, E. coli cells transformed with said recombinant DNA molecule, a protein having the amino acid sequence encoded by said gene, an enzyme which is a multimer of said protein, and a process for preparing said protein or enzyme are provided, said cephalosporin acetylhydrolase being an enzyme useful for converting cephalosporins such as cephalosporin C and 7-ACA into deacetylated ones such as deacetylcephalosporin C and deacetyl 7-ACA which are useful as an intermediate for preparing a variety of derivatized cephalosporin antibiotics. |
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| 185 | Protein | JP2152896 | 1996-02-07 | JPH08266278A | 1996-10-15 | MAACHIN KAARU RATSUSERU BAANAM; JIYON EDOWAADO HADOSON; IAN DEIBITSUDO NOOMANSERU |
| PROBLEM TO BE SOLVED: To obtain a new protein obtained from a DNA sequence having a configuration of a specific restriction cite cloned to a plasmid derived from Streptomyces, involving biosynthesis of a β-lactam compound and usable for synthesis of an antimicrobial antibiotic. SOLUTION: The objective new protein capable of converting o- carbamoyldesacetylcephalosporin C7-hydroxylase enzyme or (o- carbamoyldesacetyl) cephalosporin C into a corresponding 7-hydroxy isomer is obtained by culturing a host transformed by a recombinant vector containing a DNA obtained from a DNA cosmid library of Streptomyces clavuligerus ATCC 27064 cloned in a plasmid derived from Streptomyces or a temperate phage, having the configuration of a restriction site of the formula, and including a gene encoding one or more enzymes involving the biosynthesis of β-lactam compounds such as penicillin and cephalosporin. COPYRIGHT: (C)1996,JPO | ||||||
| 186 | Antibiotic tan-547 and its manufacturing method | JP25162783 | 1983-12-27 | JPH0699444B2 | 1994-12-07 | 節夫 原田; 英男 小野; 幸正 野崎 |
| 187 | Production of deacetylcephalosporin c and related compound | JP22149492 | 1992-08-20 | JPH06225794A | 1994-08-16 | NAKAYAMA KIYOSHI; OZAWA TATSUYA; WADA TADASHI |
| PURPOSE: To efficiently obtain deacetylcephalosporin C useful as an intermediate, etc., for synthesizing β-lactam antibiotics by reacting an enzyme source derived from a microorganism which belongs to Pseudomonas and capable of deacetylating cephalosporin C, etc., and then harvesting the reactional product from the reaction mixture. CONSTITUTION: A microorganism which belongs to the Henus Pseudomonas [e.g. Pseudomonas sp.-14-2-2 strain (FERM P-3939)] is inoculated into a sterilized medium and subjected to shake culture at 26°C at 150rpm for 96hr and the cultured mixture is centrifuged and the bacterial cell is harvested to afford an enzyme source having activity capable of deacetylating cephalosporin C or 7-aminocephalosporanic acid. The enzyme source is added to a reactional liquid containing cephalosporin C or 7-aminocephalosporanic acid and subjected to shake culture reaction therewith at 26°C for 72hr to produce deacetylcephalosporin C or deacetyl-7-aminocephalosporanic acid in the reactional liquid and then the product is harvested from the reactional liquid to provide the objective deacetylcephalosporin C and its related compound. COPYRIGHT: (C)1994,JPO&Japio | ||||||
| 188 | Production of 3-hydroxymethylcephalosporanic acid derivative | JP14257393 | 1993-05-24 | JPH0698795A | 1994-04-12 | TANI YOSHIKI; YAMAMOTO KEIZO; HAYASHI YOSHIHARU |
| PURPOSE:To convert a cephalosporanic acid lactone derivative into a 3- hydroxymethylcephalosporanic acid derivative by action of a microorganism. CONSTITUTION:A bacterium belonging to the genus Pseudomonas or Methylobacillus or its prepared material acts on a cephalosporanic acid lactone derivative to produce the objective 3-hydroxymethylcephalosporanic acid derivative. | ||||||
| 189 | Purified para-nitrobenzyl esterase derived from bacillus | JP33870692 | 1992-12-18 | JPH05292961A | 1993-11-09 | USUI SHIGEYUKI; CHIYANNAN YUU |
| PURPOSE: To obtain a new PNB esterase obtainable from a recombinant host cell, etc., in a purified form having extremely high specific activity from an extract of a bacterium of the genus Bacillus. CONSTITUTION: This PNB esterase in a substantially purified form is prepared from a recombinant host cell capable of expressing the PNB esterase. The esterase is expressed by ATCC1105/PNB106R transformed with plasmid PNB106R given by the example and is purified by ammonium sulfate fractionation, pH treatment, anion-exchange chromatography, gel filtration and adsorption-desorption chromatography. The enzyme has a monomer having a molecular weight of about 54,000 daltons, substrate specificity to loracarbef PNB ester and cefaclor PNB ester, is strongly inhibited by phenylmethylsulfonyl fluoride, diethyl pyrocarbonate, etc., and is stable at about 4-50°C and at pH about 5.5 to 8.5. COPYRIGHT: (C)1993,JPO | ||||||
| 190 | Modulation of production of secondary metabolite | JP21673191 | 1991-03-25 | JPH05199897A | 1993-08-10 | RUSHIA HEROONA MARIA FUAN DERU; RUUROFU ARI RANSU BOOBUENBERUF; WARURAABUEN HENRII MIYUURERU; ADORIANUSU YOHANESU FUERUKUREI |
| PURPOSE: To modulate the number and/or size of organelles by the treatment such an altering the expression of proteins existing in the organelles, thereby modulate the production of secondary metabolites such as of penicillin or cephalosporin. CONSTITUTION: The production of the secondary metabolites is modulated by treatment with a chemical capable of altering the expression of proteins existing in a organelles, hampering the cell modulation mechanism for the maturation or cleavage of the organelles, or modulating the number and/or size of the organelles; thereby, the number and/or size of the organelles, pref. microbodies can be modulated. In addition, by deletion or alteration of at least one DNA sequence coding for one or more target signals in at least one kind of protein gene either directly or indirectly involved in the production of secondary metabolites, the intracellular localization of the protein(s) can be adjusted. COPYRIGHT: (C)1993,JPO | ||||||
| 191 | Cephalosporin acetylhydrolase gene and protein coded with the gene | JP9516691 | 1991-04-25 | JPH04228079A | 1992-08-18 | MITSUSHIMA KENJI; TAKIMOTO AKIO; YAGI SHIGEO; SONOYAMA TAKAYASU |
| PURPOSE: To provide a new DNA base sequence usable for the preparation of E.coli capable of producing cephalosporin acetylhydrolase. CONSTITUTION: A DNA base sequence coding the amino acid sequence of formula. It can be prepared by separating a DNA fragment carrying a cephalosporin acetylhydrolase gene from Bacillus subtilis SHS0133 (FERM BP-2755) and cloning the fragment to an E. coli. COPYRIGHT: (C)1992,JPO&Japio | ||||||
| 192 | Novel cdna compound | JP4943490 | 1990-03-02 | JPH03254684A | 1991-11-13 | MATSUDA AKIO; SUGIURA KOKICHI; MATSUYAMA KENJI |
| NEW MATERIAL:The cDNA compound coding a deacetylcephalosporin C acetyltransferase precursor originated from Acremonium chrysogenum. EXAMPLE: The cDNA compound coding the protein having the amino acid sequence of formula. USE: For example, the production of deacetylcephalosporin C acetyltransferase, etc. PREPARATION: The compound can be produced by concentrating an m-RNA fraction from the whole RNA of Acremonium chrysogenum capable of producing cephalosporin, constructing a cDNA library of Acremonium chrysogenum, separating a cDNA compound coding a deacetylcephalosporin C acetyltransferase precursor and determining the base sequence of the precursor. COPYRIGHT: (C)1991,JPO&Japio | ||||||
| 193 | Improvement of enzymatic oxidation of cephalosporin c to glutaryl-7-amino-cephalosporanic acid | JP19285890 | 1990-07-18 | JPH0353879A | 1991-03-07 | JIEFURII TOOMASU BUISENJI |
| PURPOSE: To enable the yield of the subject compound to be improved, e.g. when the compound is to be produced from cephalosporin C, by selectively deactivating catalase by a specific means in the presence of D-amino acid oxidase. CONSTITUTION: When catalase is to be deactivated in the presence of D-amino acid oxidase, the catalase is contacted with a basic aqueous solution with pH 11-12. Specifically, for example, when cephalosporin C is to be transformed to glutaryl-7-aminocephalosporanic acid, by the use of an extract free from Triginopsis variabilis cell but containing the D-amino acid oxidase and catalase, the extract is previously contacted with the above-mentioned basic aqueous solution to deactivate the catalase. COPYRIGHT: (C)1991,JPO | ||||||
| 194 | Dna and its use | JP376290 | 1990-01-10 | JPH02291274A | 1990-12-03 | KIMURA HIROYUKI; MIYASHITA HIDEAKI; SUMINO YASUHIRO |
| PURPOSE: To improve the productivity of a cephalosporin antibiotic substance using a DNA fragment confirmed in a bacterial cell, separated from the cell and containing an enzyme gene group participating in the bio-synthesis of a cephalosporin antibiotic substance. CONSTITUTION: A DNA fragment originated from a bacterial cell containing an enzyme gene group participating in the bio-synthesis of a cephalosporin antibiotic substance was separated. The bacterial strain is e.g. Lysobacter lactamgenus. The enzyme gene group contains at least one kind of gene selected from δ-(L-α-aminoadipyl)-L-cysteinyl-D-valine synthetase, isopenicillin N synthetase, isopenicillin N epimerase, deacetoxycephalosporin C synthetase, deacetoxycephalosporin C hydroxylase and β-lactamase. A microorganism transformed with a plasmid integrated with said DNA fragment is cultured and the objective cephalosporin antibiotic substance accumulated in the culture liquid is separated therefrom. COPYRIGHT: (C)1990,JPO&Japio | ||||||
| 195 | Enzymatic production of 3-substituted cephalosporine | JP10299187 | 1987-04-24 | JPS63129995A | 1988-06-02 | JIYATSUKU EDOWAADO BOORUDOUIN |
| 196 | Production of beta lactams by enzyme | JP10299087 | 1987-04-24 | JPS6363393A | 1988-03-19 | JIYATSUKU EDOWAADO BOORUDOUIN |
| 197 | JPS6260079B2 - | JP8271785 | 1985-04-19 | JPS6260079B2 | 1987-12-14 | DANIERU BUZAARU; ABURAHAMU UEEBAA |
| 198 | JPS6251117B2 - | JP5107279 | 1979-04-24 | JPS6251117B2 | 1987-10-28 | FUJISAWA YUKIO; SUZUKI MASARU |
| 199 | JPS62501677A - | JP50058286 | 1986-01-10 | JPS62501677A | 1987-07-09 | |
| 200 | JPS6225358B2 - | JP10944178 | 1978-09-05 | JPS6225358B2 | 1987-06-02 | FUJISAWA YUKIO; SUZUKI MASARU |
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