人类肝脏中表达的表达序列标签G组
阅读：896发布：2020-05-12
专利汇可以提供人类肝脏中表达的表达序列标签G组专利检索，专利查询，专利分析的服务。并且本 发明 公开了一类在人类肝脏中表达的表达序列标签的序列。利用本发明的在人类肝脏中表达的表达序列标签，可以方便的寻找出在人类肝脏中表达的相关基因，从而在研究肝脏 疾病 的致病机理以及开发 治疗 肝脏疾病的药物中发挥重要作用。，下面是人类肝脏中表达的表达序列标签G组专利的具体信息内容。
1.一类在人类肝脏中表达的表达序列标签的序列，其特征在于，它包括：
(a)SEQ ID No.1～SEQ ID No.63所示的序列；
(b)SEQ ID No.1～SEQ ID No.63所示的序列中每条序列的互补序列；
(c)与SEQ ID No.1～SEQ ID No.63所示的序列中每条序列有至少70％同源性的 序列；
(d)上述(a)～(c)中一条或数条的组合。
2.如权利要求1所述的表达序列标签的序列，其特征在于，所述序列包括具有SEQ ID No.1～SEQ ID No.63所示的序列。
3.一种探针分子，其特征在于，所述的探针分子含有权利要求1中所述的序列中约 8-100个连续的核苷酸。
技术领域
本发明涉及生物技术领域，尤其涉及一类在人类肝脏中表达的表达序列标签。
背景技术
肝脏是人体内最大的消化腺。也是体内新陈代谢的中心站。据估计，在肝脏中发 生的化学反应有500种以上，实验证明，动物在完全摘除肝脏后即使给予相应的治疗， 最多也只能生存50多个小时。这说明肝脏是维持生命活动的一个必不可少的重要器 官。肝脏的血流量极为丰富，约占心输出量的1/4。每分钟进入肝脏的血流量为 1000-1200ml。肝脏的主要功能是进行糖的分解、贮存糖原；参与蛋白质、脂肪、维 生素、激素的代谢；解毒；分泌胆汁；吞噬、防御机能；制造凝血因子；调节血容量 及水电解质平衡；产生热量等。在胚胎时期肝脏还有造血功能。
肝脏疫病分为肝炎、肝硬化、脂肪肝、肝癌等。现代医学实验证明，肝病病毒侵 入人体后，并不直接引起肝细胞的损害，只是在肝细胞内吸收营养赖以生存，并在肝 细胞内复制、繁殖。其复制病毒的“零部件”如表面抗原(HBsAg)、e抗原(HBeAg) 释放在肝细胞膜上，引起人体免疫系统对这些抗原物质产生免疫反应，这种反应造成 肝细胞的损伤、坏死。免疫反应的强弱决定于肝脏受损程度及临床症状轻重。这场由 病毒引发的、免疫系统对肝细胞的战争，使大约25％的患者的肝脏成为战火连绵的战 场，肝脏的损伤由此加重。肝病的危害绝不仅仅限于肝脏本身，它还可以引起其它多 种疾病。常见的有：(1)糖尿病；(2)胰腺炎；(3)胆道感染；(4)功能性肾衰竭； (5)胆汗性肾病；(6)肾小球肾炎；(7)肾小管酸中毒；(8)溶血性贫血；(9)再 生障碍性贫血；(10)心肌炎和心包炎；(11)结节性动脉炎；(12)消化性溃疡；(13) 自发性腹膜炎；(14)性激素代谢紊乱；(15)甲状腺功能改变；(16)肝性骨病，等 等。肝病不仅对患者的身体甚至生命造成危害，而且对患者心理上的打击也是十分沉 重的。无论是肝病患者还是病毒携带者，在生活、社交、求职、升学等方面都会受到 严重影响。
生物基因组中可转录表达的序列(即基因)仅占总序列的3-5％，对这部分序列 进行测定，将直接导致新基因的发现，并获取基因组中与产业化关系最为密切的信息。 20世纪80年代，高通量的自动测序的出现，使从质粒互补脱氧核糖核酸 (Complementary DNA，简称cDNA)文库随机选取许多cDNA克隆和决定来自非载体两 端的几百个碱基的DNA序列成为可能。这些短的DNA序列叫做“表达序列标签” (Expressed Sequence Tags，简称ESTs)。表达序列标签的概念最早是由Adams等在 1992年提出来的(Nature，355，642-644)。1992年Sikela和Matsubara(Sikela， et al.Nucleic Acids Res.19，1837-1843；Matsubara，et al.NAture Genetics， 2，173-179)针对获得大量信使核糖核酸(mRNA)序列的迫切需要，提出大规模互补 脱氧核糖核酸(cDNA)测序的研究战略。随后Venter创立了大规模表达序列标签技 术。其基本特征就是从以质粒为载体，构建完成的目的组织互补脱氧核糖核酸 (Complementary DNA，简称cDNA)文库中，随机选择许多cDNA克隆，利用质粒上携 带的通用引物对cDNA两端进行一轮脱氧核糖核酸序列测定，所获得的来自3’端或 5’端的几百个碱基的非载体短脱氧核糖核酸(DNA)序列。简而言之，表达序列标签 是来自表达基因片段3’端或5’端的短脱氧核糖核酸序列，代表一个表达基因的部 分转录片段。
表达序列标签可用于新基因克隆、人类基因组图谱绘制、基因组序列编码区的确 定等。如果一个表达序列标签在基因组中只出现一次，那么它可以作为序列标签位点 (STS)。由表达序列标签构建的物理图谱叫表达图或转录图(expression or transcript map)。利用表达序列标签进行基因图制作，可以加快序列标签位点的制 作和新基因的染色体定位。表达序列标签可以作为基因特异性探针，对组织特异性基 因表达的研究具有重要的作用。表达序列标签还可以进行新基因的遗传进化关系分 析。表达序列标签可以对所有动植物的基因作为一种数据库，通过不同的序列比较可 以获得保守序列片段，从而获得基因的遗传进化图谱。正因为表达序列标签具有如此 的优越性，因此表达序列标签测序已经成为许多基因组研究机构的工作重点。
表达序列标签(EST)是一种快速有效揭示基因组容量的方法。ESTs在新基因的 发现、基因作图和基因组序列编码区域的确定等方面起到重要作用。EST不仅为基因 组遗传图谱的构建提供了大量的分子标记，而且来自不同组织和器官的EST也为基因 的功能研究提供了有价值的信息。此外，EST计划还为基因的鉴定提供了候选基因 (candidantes)。
在本发明之前，还没有出现涉及本发明的一类在人类肝脏中表达的表达序列标签 的公开报道。
肝脏疫病分为肝炎、肝硬化、脂肪肝、肝癌等。现代医学实验证明，肝病病毒侵 入人体后，并不直接引起肝细胞的损害，只是在肝细胞内吸收营养赖以生存，并在肝 细胞内复制、繁殖。其复制病毒的“零部件”如表面抗原(HBsAg)、e抗原(HBeAg) 释放在肝细胞膜上，引起人体免疫系统对这些抗原物质产生免疫反应，这种反应造成 肝细胞的损伤、坏死。免疫反应的强弱决定于肝脏受损程度及临床症状轻重。这场由 病毒引发的、免疫系统对肝细胞的战争，使大约25％的患者的肝脏成为战火连绵的战 场，肝脏的损伤由此加重。肝病的危害绝不仅仅限于肝脏本身，它还可以引起其它多 种疾病。常见的有：(1)糖尿病；(2)胰腺炎；(3)胆道感染；(4)功能性肾衰竭； (5)胆汗性肾病；(6)肾小球肾炎；(7)肾小管酸中毒；(8)溶血性贫血；(9)再 生障碍性贫血；(10)心肌炎和心包炎；(11)结节性动脉炎；(12)消化性溃疡；(13) 自发性腹膜炎；(14)性激素代谢紊乱；(15)甲状腺功能改变；(16)肝性骨病，等 等。肝病不仅对患者的身体甚至生命造成危害，而且对患者心理上的打击也是十分沉 重的。无论是肝病患者还是病毒携带者，在生活、社交、求职、升学等方面都会受到 严重影响。
生物基因组中可转录表达的序列(即基因)仅占总序列的3-5％，对这部分序列 进行测定，将直接导致新基因的发现，并获取基因组中与产业化关系最为密切的信息。 20世纪80年代，高通量的自动测序的出现，使从质粒互补脱氧核糖核酸 (Complementary DNA，简称cDNA)文库随机选取许多cDNA克隆和决定来自非载体两 端的几百个碱基的DNA序列成为可能。这些短的DNA序列叫做“表达序列标签” (Expressed Sequence Tags，简称ESTs)。表达序列标签的概念最早是由Adams等在 1992年提出来的(Nature，355，642-644)。1992年Sikela和Matsubara(Sikela， et al.Nucleic Acids Res.19，1837-1843；Matsubara，et al.NAture Genetics， 2，173-179)针对获得大量信使核糖核酸(mRNA)序列的迫切需要，提出大规模互补 脱氧核糖核酸(cDNA)测序的研究战略。随后Venter创立了大规模表达序列标签技 术。其基本特征就是从以质粒为载体，构建完成的目的组织互补脱氧核糖核酸 (Complementary DNA，简称cDNA)文库中，随机选择许多cDNA克隆，利用质粒上携 带的通用引物对cDNA两端进行一轮脱氧核糖核酸序列测定，所获得的来自3’端或 5’端的几百个碱基的非载体短脱氧核糖核酸(DNA)序列。简而言之，表达序列标签 是来自表达基因片段3’端或5’端的短脱氧核糖核酸序列，代表一个表达基因的部 分转录片段。
表达序列标签可用于新基因克隆、人类基因组图谱绘制、基因组序列编码区的确 定等。如果一个表达序列标签在基因组中只出现一次，那么它可以作为序列标签位点 (STS)。由表达序列标签构建的物理图谱叫表达图或转录图(expression or transcript map)。利用表达序列标签进行基因图制作，可以加快序列标签位点的制 作和新基因的染色体定位。表达序列标签可以作为基因特异性探针，对组织特异性基 因表达的研究具有重要的作用。表达序列标签还可以进行新基因的遗传进化关系分 析。表达序列标签可以对所有动植物的基因作为一种数据库，通过不同的序列比较可 以获得保守序列片段，从而获得基因的遗传进化图谱。正因为表达序列标签具有如此 的优越性，因此表达序列标签测序已经成为许多基因组研究机构的工作重点。
表达序列标签(EST)是一种快速有效揭示基因组容量的方法。ESTs在新基因的 发现、基因作图和基因组序列编码区域的确定等方面起到重要作用。EST不仅为基因 组遗传图谱的构建提供了大量的分子标记，而且来自不同组织和器官的EST也为基因 的功能研究提供了有价值的信息。此外，EST计划还为基因的鉴定提供了候选基因 (candidantes)。
在本发明之前，还没有出现涉及本发明的一类在人类肝脏中表达的表达序列标签 的公开报道。
发明内容
本发明要解决的技术问题是提供一类在人类肝脏中表达的表达序列标签。
为了解决上述技术问题，本发明通过如下技术方案实现：
在本发明的一个方面，提供了一类在人类肝脏中表达的表达序列标签的序列，其 包括：
(a)SEQ ID No.1～SEQ ID No.63所示的序列；
(b)SEQ ID No.1～SEQ ID No.63所示的序列中每条序列的互补序列；
(c)与SEQ ID No.1～SEQ ID No.63所示的序列中每条序列有至少70％同源性的 序列；
(d)上述(a)～(c)中一条或数条的组合。
较佳地，所述序列包括具有SEQ ID No.1～SEQ ID No.63所示的序列。
本发明还提供了一种探针分子，所述的探针分子含有上述序列中约8-100个连续 的核苷酸。
由本发明的一类在人类肝脏中表达的表达序列标签，可以方便的寻找出在人类肝 脏中特异表达的相关基因，从而在研究肝脏疾病的致病机理以及开发治疗肝脏疾病的 药物中发挥重要作用。
为了解决上述技术问题，本发明通过如下技术方案实现：
在本发明的一个方面，提供了一类在人类肝脏中表达的表达序列标签的序列，其 包括：
(a)SEQ ID No.1～SEQ ID No.63所示的序列；
(b)SEQ ID No.1～SEQ ID No.63所示的序列中每条序列的互补序列；
(c)与SEQ ID No.1～SEQ ID No.63所示的序列中每条序列有至少70％同源性的 序列；
(d)上述(a)～(c)中一条或数条的组合。
较佳地，所述序列包括具有SEQ ID No.1～SEQ ID No.63所示的序列。
本发明还提供了一种探针分子，所述的探针分子含有上述序列中约8-100个连续 的核苷酸。
由本发明的一类在人类肝脏中表达的表达序列标签，可以方便的寻找出在人类肝 脏中特异表达的相关基因，从而在研究肝脏疾病的致病机理以及开发治疗肝脏疾病的 药物中发挥重要作用。
具体实施方式
下面结合具体实施例，进一步阐述本发明。应理解，这些实施例仅用于说明本发 明而不是限制本发明的范围。下列实施例中未注明具体条件的实验方法，通常按照常 规条件如Sambrook等人，分子克隆：实验室手册(New York：Cold Spring Harbor Laboratory Press，1989)中所述的条件，或按照制造厂商所建议的条件。
实施例1
人肝脏组织的mRNA的分离
组织分离(Tissue isolation)：肝脏来源于5个成年男性，在肝脏切除手术后， 将肝脏组织立即置于液氮中冷冻保存。
mRNA的分离(mRNA isolation)：取出肝脏组织，用研钵研碎，加入盛有裂解液 的50ml管，充分振荡后，再移入玻璃匀浆器内，匀浆后移至50ml新管，抽提总RNA (TRIzol Reagents，Gibco，NY，USA)。用甲醛变性胶电泳鉴定总RNA质量。用带Oligo d(T)的纤维素柱分离总RNA中的mRNA，定量。
实施例2
cDNA文库的构建(Constuction of cDNA library)
以mRNA为模板，合成双链cDNA。补平末端后，加含EcoRI切点的接头。磷酸化 EcoRI末端后，用XhoI限制性内切酶消化1.5小时，再进行片断分离。过柱筛选长 度＞500bp的片段，用酚-氯仿抽提，乙醇沉淀，无菌水溶解，连接至Uni-ZAP XR载体 (Strategene，CA9203，USA)，以ZAP-cDNA Gigapack III Gold Cloning Kit (Strategene，CA9203，USA)进行包装，宿主菌使用XL 1 Blue MRF’ (Strategene，CA9203，USA)细菌。涂板并测定滴度。
实施例3
测序及数据库建立(Seqencing and Database Constructing)
挑选文库中有外源片段插入的克隆，扩增后抽提质粒(Qiagen Germany)，用T3 和T7作为3’和5’端的通用引物，采用终止物荧光标记 (Big-Dye，Perkin-Elmer，USA)的方法，在ABI 377测序仪(Perkin-Elmer，USA)上 进行EST大规模测序。测序结果用FACTURA软件去除载体序列，传输到SUN Ultra 450 Server上进行下一步的处理。所有的序列信息再用GCG软件包(Wisconsin group，USA) 中的BLAST和FASTA软件搜索已有的数据库(Genebank+EMBL)，将无同源性或同源性 低于95％的序列视为新基因建立数据库。
实施例4
基因的全长克隆(Cloning of Full-length cDNA)
在得到的新基因片段序列信息基础上，进行cDNA全长克隆，分两阶段进行：
(1)“电子克隆”(Electronic Cloning)
以新基因片段序列作为探针搜寻dbEST数据库，将重叠序列＞50bp，同源性在98％ 以上的表达序列标签(Expressed Sequence Tag，简称“EST”)序列认为同一序列 (Consensus Sequence)，取出并用AUTOASSEMBLER软件进行连接，部分EST可以延 伸探针序列。再用STRIDER软件分析被延伸的序列是否具有完整的开放阅读框架(Open Reading Frame，ORF)，用BLAST搜寻Genbank或SwissProt以确定该序列的核苷酸 和氨基酸水平上是否与其他物种有同源性，以帮助判别所得到的基因全长完整性如 何。通过电子克隆的方法，通常可获取人肝脏相关基因的全长序列。
(2)cDNA末端快速扩增(Rapid Amplification of cDNA Ends，RACE)
如果通过“电子克隆”方法仍未得到完整的cDNA全长，则在已有序列5’或3’ 端设计引物，在人类肝脏Marathon-Ready cDNA文库(Clontech Lab，Inc，USA)中 进行长距离PCR反应。然后对PCR产物克隆、测序。用AUTOASSEMBLER及STRIDER软 件分析被延长的序列有无完整的ORF，如无，重复上述过程直至获得全长。
(3)RT-PCR
对于5’和3’端的已知的序列，如果中间有一段间隙(gap)无法从已有的公共 数据库或自身数据库获得，可考虑采用RT-PCR的方法。在序列5’端设计引物，3’ 端引物采用Oligo-dT，在肝脏总RNA库中进行扩增。然后对产物进行克隆、测序。最 后拼接便获得全长。
通过组合使用上述3种方法，可获得人肝脏相关蛋白的全长编码序列。
序列表
<110>上海人类基因组研究中心
<120>人类肝脏中表达的表达序列标签G组
<130>NP-10043
<160>63
<210>1
<211>566
<212>DNA
<213>Homo sapiens
<400>1
1 tgtcgtagag ctttttattt tgcatctgga gtgtatttgg acacgtgggc tgggtggggt
61 aacaggataa tgagcagttt gggaaagagg caggctccaa gcaggctggt tcactcactt
121 ggcagtcact gggcggctat tcttgtgcct ggatgtgtta caatgcaggg gacacagtgg
181 tgagcaaggc tgctccaagc cctggctggg atgggggatg cacagacagg ctgatcaggg
241 ctgggatggt ggacacgcat cctctttacc ctgatcactg gttagaaata ctgcactgtg
301 gccgggctca gtcggctcac acctgtaatc ccagcacttt gggaggccga ggcgggttgg
361 attactcaag gtcaggagtt cgagaccagc ctggccaaca tggcaaagcc ccgtctctac
421 taaaaataca aaaaattagc caggcatggt ggtgcatgcc tgtaatccca gttacttggg
481 ggggctgagg caggagaatc acttgaacct gggaagtgga ggctggagtg agctgagatc
541 acgccaccgc actccagctt ggacga
<210>2
<211>492
<212>DNA
<213>Homo sapiens
<400>2
1 gcatgtacaa agctattggt ttattcagct gccaantggt ggaaaggctt cctttcgaag
61 tgaatagcaa aaatgcattc atgagcaagg cacttttata cacacacaga gagaaaaagg
121 aacgtattat gaacacatga acataaacat attttaatat tcagacctct tccctacctc
181 ctcccacctc cacttaaaca aacaaacana aaaaaaggaa acagtttcct gtaacatttt
241 agaaaccagt ttcacagaat acctacatag gaacaatttc tgatacattc agttacacta
301 acnntatgat atggtttcag gtcaataatt tccactaaca aatactttta ttctacaatt
361 ccctctnttt ttccttttag acaagacaac ttnaggctca gcngaaatat ttcactaggt
421 taataaggca ttcttctctc ttcttaaagt tgctatatat ttacncaatt ggtgnttcct
481 aacctttttt tt
<210>3
<211>683
<212>DNA
<213>Homo sapiens
<400>3
1 gttgttgcca attgtttttg ttgtcttcca gaggtaatac aaggctttgc tcctttagca
61 ggattcccag ttggaccctc tccagagagg attcatattt gaattcccat ctgaatacca
121 acccaaatgt tgatacagaa cactcctgta ttaaaattaa tatccatccc agataaacct
181 actctgtgac taagacaatt gagatcttct aggtgaagat gctataattc aaaatattac
241 atggaaaacc atgtcttact taaaacgggt acttgttttc cggccataat tattccagtc
301 tcttccacag aactgcttct gcaaacagtt tttttaatgt atcaaagaga gtctctcgcc
361 aacatttaat acagtcaaat ctattccaac ttcagagttc ttatatgtct tatttagcag
421 acactatgat tctatcttct tattctctgg aaatccatca gatgtgtgtt ccaacacaga
481 agtgccttcc ttccttctca tggtggcaaa agcatatcca tcatccccaa ggttntaaca
541 atcccttggt aatcaactct tttctacaag ccctgaagag taaacnttta tcactacagg
601 gctgaccatt caggggaatt nccataattt tcagcgctga taacaggnaa ctgcggaaat
661 ccccggcgat ttgccgggna atc
<210>4
<211>592
<212>DNA
<213>Homo sapiens
<400>4
1 gcactaatgc tgcttttaat ggcggcgcaa tagagaagta caaggtttta ctagcttgag
61 ctactagaag gtgtaaaaga tgcccgctga cactaccaag tagcctctta atagaaatgt
121 agaaaatata caggacaggg tggagatgag ctcttgaatt tagtagggtg aaagagttaa
181 ctgccaaccc ggcactatca ttgtacttgt ttggagaaaa tctgttttgt tctggtgatg
241 atggttttat cttccctttt agttgggtgg gaagtaacag aaaatttgtt tccccaaaag
301 tttgacattt tcttgttaat acacgtttca tttcagtacg atgccaactg ttaagcctgc
361 aggaatcctc ctgggtattt ctgaattgtg ctgtgtgcat gtgtgtttga aagctcaaac
421 agcttgtctt cttacagcat cgagttgttt gctttattgt tagacacaat attagcagtg
481 tatcctttcc gtattcccta ccatcgaacc cattaagact aaaatccttg tcaagtactg
541 gcttaaaagt tgggagatgg tggtttanaa aaaaaaagcc ttgcaaaacc at
<210>5
<211>664
<212>DNA
<213>Homo sapiens
<400>5
1 aaatggtatt catttttatt tcaacatgtc aactgtgcat ttccaaaaca gcaggctttt
61 caaaggaata aatcagaact gtaaacacaa gatacagtac aagtttttga cttcctacag
121 tcagtttcac aaatccacat actgtacatt cataggtgag gttaagcctg tcacccattt
181 ctttatttct ataattacac aagcataata aatacatctg cttttaaagg tcacttaaaa
241 tgagtcataa tttacagtac agtacgtttc agttcaagtg caaaaaataa ctatttgctg
301 aattctattt ctttcagtta ttttattttt aagctgtgtt ttattgtgaa gcgagacatc
361 caagtgtaga atttcttatc ccaaatgcag tattgggcag aactatggtg tctggagaag
421 ccctcacttt acaattgatc cgtctaatcg ggtcagaggg taaggtgagt tgctcattcg
481 actgttcttt cttcctctct ctccgtgctc atccacagct attataaatt atataattca
541 ctttattcag ctaaaacagt ttccctttcc acactncccc caggtttccc gttaaccctg
601 cttgatttta gtggcagctt acgtggatca nccaccatgt ctggngcatg gacggtgcgg
661 tgca
<210>6
<211>449
<212>DNA
<213>Homo sapiens
<400>6
1 tacatttggt tatgtgttta attacaagtg tttcaatagc tataaacatt gtaaaattca
61 gtatcaagtg aaatatttgg agagccccta tggcaacttg gaaaaaccct gggattctga
121 ggaacaggga gtagcgcacc agtcccagta aaatccagaa ctttctagaa ggagataaat
181 gcaggatgac tgcctgcttg ccctgggcca gctcagccag aaaaccatgt ccctgaaaga
241 tgggaaactg gttccttgat acagcagaaa aacactgatt ctaccccaaa tgtactcaca
301 agacagaaga acgtgcatat gtcaaggagg aatgttcaat ggctgggcac caggcttaaa
361 atatccgctt gacccagtgg cttaaaagca gttccttctg aattgaacag agaatccctg
421 cggagatcaa aggagcctca gctcgtttn
<210>7
<211>428
<212>DNA
<213>Homo sapiens
<400>7
1 gaggtggttt atgtgcccca tagccaagag aggtgngcac caaaggagga tatcatcaaa
61 tctgacaatc tggaaagcct ttgaaactgt tcttttccta agcacagtat tcagctgtgt
121 cctcttgaac ccatatctat caggtcaaca gctttagccc attccacatg atattggctg
181 tgggtttgtc atatatagct cttattattt tgagaaacgt tcnatcaata cctagtttat
241 tgagagtttt tagcatgaag ggcttttgaa ttttgtcgac ggccttttct gcatcnattg
301 agataatcaa gcggtttttg tcattggttc tgtttctgtg atggattatg tttantgatt
361 tgcatatgnt ggaaacagcc ttgcatccca gggatgaagc ccancctcgt gccgaattcc
421 tgggcctc
<210>8
<211>332
<212>DNA
<213>Homo sapiens
<400>8
1 tttatcagtt gaaatacttt atttaactta ttttaaaaat tgattcaaaa ctttttatct
61 tctttattag gtattctacc cttagattta acactcattt ttcccgagta tattttctct
121 tcaatataca aaggaatata ttttcataga gtgtgttgaa tattaggaaa atcaagagtt
181 ctggttctgt caggtgcagt atttagagtg ttattttgat aaaatccagt tgctctgata
241 tggcttgaaa atacttgctc ctttggagta taattgcagg ggaaaaactg tggaataggt
301 agaaattcac cataatgact tgaattggtt tt
<210>9
<211>428
<212>DNA
<213>Homo sapiens
<400>9
1 tttcaggcaa ctgcttctct tttattatga atatgatttg aatctaccca ctgcacactg
61 aggctactgc ctctctttat aaaaaggaag gaagaaattt ccgtttcccg cagatgtgat
121 gtaggaagct acaactgcca actcctgagc atgagggagg ggacaggaga atcactgagg
181 ccctgacccc aaggcaaccg agacttagct gtgtgcccac agctgccctc gccaggcttg
241 ttttgtgagg gaataatcct tagtgcttaa tccattactg ggggagtttt gctattgtta
301 agaaatgtca gcattaaaaa actaggagac ttcacataaa aatacaggga tgtggatccc
361 ctgtactcta tctagggtaa gcctgcgcaa cagtggacta acaatgggga tcccagtgga
421 gagagggc
<210>10
<211>304
<212>DNA
<213>Homo sapiens
<400>10
1 gagagcttag atagagtgta tttttattct tttttttcca tttccttttc tcttttcatc
61 ttttttcttt ctttctttct ttcctttttt tcctttgtat ttttatctta acatgcctag
121 acagagaaga acactggatt gtacataatg ggctttcttt gactctcttt tccagagtgg
181 aaataggatt ctgtgctcca tcattctgag ccatcaccag gggccgtcca taatcaacta
241 tgatgatgag agtgggaaga catgtgtaca tatcgcagcg gcaggtgctt cagcgatatt
301 attc
<210>11
<211>487
<212>DNA
<213>Homo sapiens
<400>11
1 taaaacttcg aaatatttat tattaagata taaatacata ttttaaaaat gtacaagtta
61 aaaatacctt caaagttaaa tgtcagttct ttcacaatca ttgtgccggg gtaatacgta
121 caatatcttg gcttactagt aattttatac cagtcacttg aaacaacatc cttaaacttt
181 aataatatgt tgtaataatt ccatttttca ctttttagag tttgatagaa gacagtcaat
241 atcgtgaagg atttcagagg tttttttcaa tgctattgat actctatttt cattaacttc
301 tcctagcaag gtttctgaag atcccgggtt tataatgatc aaaagactgt tgttcatttt
361 tcttgttaga aatctggttt aggataccac tattttctag tagcaagtta aacttctgta
421 tcttttcaag ttcagaagca acagaccaat tttcttggag aacagaagat gacgaatgag
481 tgggcat
<210>12
<211>591
<212>DNA
<213>Homo sapiens
<400>12
1 tttaggctga tcatactaga gtatatttat taaatttcac cattatgatc actttgttat
61 aactaaatta accctgacat aaactttgac attttaaaaa cagagaacat actgattttt
121 caactatttt acttacatgt gttttttaat tctcatgcag tgctcataat taggagatac
181 tacatattca ccctcacatt atgcttatta agaaccaagc tcagagggta gaaaagtgcc
241 aacttcattt tccataaact tcccatacat atattaagcc actgttcttc taattatata
301 cttatggtac attcttctgt aaaatgtata actgtagtca cataaccatt gttatgacaa
361 ggcggcaaca tcatttcaga ttacaacaaa tgagaccaaa agttgaagag accacagcat
421 ataaaagtca tgagaacacc acacacattt caatattttc cactttccag tcaaaaaaac
481 atgcacaaag ttttcttagc agatagttgt cagaagtgca taaaaaaact ggctttcagg
541 tcatatatgt tgagtgagga tcaatgccat tgcgtgaaga gagctgtgtt c
<210>13
<211>755
<212>DNA
<213>Homo sapiens
<400>13
1 gcaccaaagt gacttgctta ttttttttat taaatgatac attgaaagta taaaaagttg
61 ccgcagtaca aacaaattac agaataatgc atataaaagc gctatggact acacgtgaca
121 attctgctta acttccatct tgatgatttt atatcagtat ccaaaattat atactccagc
181 attttttccg aatatagtct tacatgattg caattcctga ggtaaactaa gtgtccactg
241 ccaacatcct tggacgaaaa ccaagtcccc cgggagaatt cagatattta tattcacaca
301 tacaatacaa cggaattcat caatgtaatc agatattgca gatttaaaaa tggaaaatgg
361 actcttgcaa tacttctgca tccatatata attttaccag cagggcctcc cagaatggtg
421 tgttagttta aatggatttc agtaataggt ttctgcattt agatagacat tcttggctaa
481 tctgnntaga gtgacactga ctagcattgc gaacagaaat caaagttttc acatttgcaa
541 gcagcacaga ttgtccctta aagaggagca atttgctact acagtcctac catcttctca
601 tgaaaacttt agcaaaggtg tcatgttagg ttgcggcaaa ttaattcgaa atccacgact
661 ttccatcagg gcgtcacaca agtcgtgttc cagaagagcg tgtcctcata aggctcttaa
721 attcgagtgc gatggagcca tctattcgtc cagaa
<210>14
<211>555
<212>DNA
<213>Homo sapiens
<400>14
1 tttaccataa ttgacttttt atttaaaaaa ttacacggag caatttccag cttatctttt
61 tttataaaag tactgcctat atcaaacatt ttatatcacg ttaattccat tgaagagctg
121 cctttttctg ttaaggtact gattccaatt gatgggatac atgcccttaa tacagaaagt
181 ttccattatt tattcaaata tcaaaattaa gattattgag aagtttattg ctttatggct
241 gggcaagatg ctactagcac attttaggta aataatattc tttattaaaa actatgaggt
301 cattctgttt aaaactttca agataattca cggaaaacag atatatctat tcaaattaaa
361 cattaaaggg ccaggctact agctatagac atttatatgt gaagcagctc tttttttttc
421 ttttctgaga tcgagtctca ctctgtcgcc cagggtggag tgcagtgggc caatctcagc
481 tcactgcaac ctccggctcc aggggttcag caattctcct gcctgggccc ccaagtagct
541 cggaatacag gcgcg
<210>15
<211>476
<212>DNA
<213>Homo sapiens
<400>15
1 tttttttttc acggacagac agggtcgttt gtcacagcag agaagcacct cacggctcct
61 acccgcactc atcgcggaca gtgcctgcag cgggacgacg cagagcaccc tccccagatg
121 aaaacaccag caccaggagg tgggccgtag ccagagctga gggaggaggc tgggggctgg
181 ggctcagggc cccccccggc cacagcgcca ccctgagtgg ccctgaaaat agtgcacagt
241 gctgggtact gccccggccg gacgacacta gttgttgagc attccggcca caggcacacc
301 gctggccctt ccttggtgtg gcacgagacc acgggcactt gcaggagctc cctgcatgct
361 gttttgtgct ttggtctcag ggagcaccct cctacctcgg ggtcccagag tgggcagccg
421 ggcaggtgtg aacagtgtga caagggtacc gtggggcacc tggtagtgca cccaga
<210>16
<211>390
<212>DNA
<213>Homo sapiens
<400>16
1 ttatattcgc ggccgggcgg cgtgtttttt tttttttgct gcatatatag ctgtggtaca
61 ttttaatgtg aaatgatgac tgcatcagct tatatccatg gagcagattt tagcattcag
121 cttgggtctc ccagtcaata tctacgagtc tcttcttaag gagatcgatg acacagatac
181 atacagacta acaaatgtga taccaataat caagaattca ctcagttaag attttgccca
241 ctgatttcca cacaagaaac ctagaattta ctagattctt gtgcctgtga ggctccactc
301 atttccctga atcacaaaag ctacagagta tttagataga aatataccta ctcttaacat
361 gaaccatttt aaatatatgt attactgtgt
<210>17
<211>514
<212>DNA
<213>Homo sapiens
<400>17
1 tttttttttt tcctttaata cagaaacttc attgaaaaaa cctagccctg tacagctgcc
61 cttttaaaga ggtggccatt gcagggtctc tgcctcccca gcaggtggca gatgctcccg
121 gccactcgtt taaataactg tcccctggag gggnccgggg tttgaaggca gggccctgcc
181 accctcctgg gggacacccg tgcatgttta cgccttttct ggttgtgtca gtggccggag
241 catggcagct gggcatcaat aaacacctcc gaggaagctg gactcgtgtg tgctggcacg
301 gggagggcgc cgggcggggc cgtggtgggc atggcgtgat gtcacggtca ctccgggatg
361 tcgatcacca ggtcatcgtc tccatccaag gcatcgtccc cgctacctgc atcgctgaac
421 atctgccgag ggaccgtgct caggatcgtg ggggtctcac aaaccctcct agagagctct
481 gtgggtctct ctgcagtccc gtgctttgtc tgtg
<210>18
<211>502
<212>DNA
<213>Homo sapiens
<400>18
1 ttttttctcc ctcggcagga tatcccacag caagggcatc ttgaaaagca tgattttctc
61 ggtaatgttt gccaacactg ttcactttcc acatggtcac gactgaaaac acatttacca
121 atacctttca agcgatatga ctaccagaaa tagatcttct ttactaccct ctctgaaatg
181 agtacacaag aaataaattc agaaggtagg cttttgaaag aaaaagaaaa aaaattgctt
241 gcggcttcac agtgaaaaaa attggagtgt ttgtgccggt taagatttca atgttttctt
301 aatcaaaatt ctctgaagtc attctcaatt tacaaggtta gaactccttc aaagacaaga
361 catacaagac tctagcttta attatcattc taaatgtcca ctagccctgt atctttggtc
421 atgaaagcag caaaacattc acattttcat catactacat tcaattgcct tccaagttta
481 gaccattcta cattttaaaa aa
<210>19
<211>507
<212>DNA
<213>Homo sapiens
<400>19
1 tttttttttc agggcaggcg acctaatgta ggaaacttaa taaacccttg cattgatata
61 atgatctgtc aggttttcat ttgaacagtt ctccaacatt ctctgaactg ccaaataaac
121 tgaatctaag ccctattttt tcttatgcta aagtgtgaat gattctggac tatcttactg
181 ggaaaacatt tcctaacaat gtttttaaaa gtcataaggt caaaactgca gttataagaa
241 agaagggtga atgcagtggc tcatgcctgt agttccagtg ctttggaagg ctgaggcggg
301 aggactactt gaggccagaa gttcaagacc atcctaggca acacagcaag acctgtctct
361 acaaaaaaaa attttaaaat tagccaggtg tgacagtgca gaaaggtgag gtgagaggag
421 cacttcagtt caggagtttc aaggctgcag tgagctatga ctgtgccact gcattcctgc
481 ctgggtgaca aaatgagact ctatctc
<210>20
<211>557
<212>DNA
<213>Homo sapiens
<400>20
1 tttttttttt ttagcagccc ccacataatg actttatttg cttaatcccc aaattaaacg
61 ccgtttcaag cgagggtcct gtgttattac tctcatcatg tcgagaacat tttcctgccg
121 agaccaattt gaataaaaca aggggccttc cttgaactca atcaaggagc cataatgtgg
181 tgggtaatag aggttgctga tagatttgca ggcaaaagtg ttatacctaa tgatgggata
241 tggataatag tcctcctgga ggtcctgatg cgattccagt tggctgccag atgcttggct
301 cgaatgcgaa aattctccag gtgtctctga cgtgattcct ttagtatctt ctttagtcga
361 gtcttatggc aatataacag ccaagcaatg gcaatcacac ctgatgccca cttctgctgg
421 cgataaaaga ggaagaattt tcttgctttg tagcatttcc atgtggcttg gatcttcatg
481 gcggcctccg aatttccatc ttggcccttg tacctttgcc ctggaagatt tatcagcatt
541 tggacatgag ctgggtc
<210>21
<211>222
<212>DNA
<213>Homo sapiens
<400>21
1 tttttttttt tttttttttt tttttttttt tttttttttc ctttgacagt gaccatttat
61 tacacacatc agactggggg aagaccattg acatagctga gcaccaggta gggtgtcctg
121 ggtattcggg catgagagca gcaggggcct cctgacgcgg gcaggagaaa catggcaccc
181 accaggagga ggaagaggag gaagaggata aggaggaagg tc
<210>22
<211>534
<212>DNA
<213>Homo sapiens
<400>22
1 aaaaggattt aaaagtcttt atttcctctg aaaacttact ggtaaagtat acaatttatg
61 aataatcata tattcatgct ttttgaaata tatctgaata aaagacaaat tattcaatat
121 caatagcagt aaataactta tttgcaagaa aattattaca attcctactg taaagaatca
181 ccaaccacta tttagcaatt aaatttcaac acattttttt tggaactctt taggacttta
241 gacaaaggct accaaggctg acaatccagg taactatggc agtgacagaa gtcaatcatt
301 aatactgcca ttattttaga caaagggctg acaatctcag aaactagttc tagaaattat
361 catcaatgtg gccattttac tatggacaaa taaatatggg agatagggaa cttgttactc
421 cattttccta tagaaaaata tataaattga ccttttatat atacacaggt atgtatacac
481 atacagtata tgtttttctg agcaggcaca ccaactagga aaaaatacaa tagc
<210>23
<211>497
<212>DNA
<213>Homo sapiens
<400>23
1 ttttcacttg tttaaaacat ataatgcgat ggttttggta tttacagagt tgtgcaatat
61 cactacaatc taactttaga acattttcct taacccctaa ataaacctca tcctcatcag
121 cagtcacttc ccatccatcc caccccaact ccaaggccca aggatccact ttctgtctct
181 gtggatgtgc ctacggaatc tgtttctcaa cacacttctg ggtgattctg ctcctgcagg
241 tccacggtcg agcattcagg aaccagtgga ccagactgtt ccaagatcct ttcctagtct
301 aagctttcat agccggaaca atgaataccg acgaatangc aggtgctttg cttggtgctg
361 gggttgtaaa agaaatatgg tacaccctac tcttgagaac ctacagtcag tgggaaaaat
421 ggaatcataa atgactaact ttaatacaat gtgagaagcg ctctaacagc atgtgcattc
481 aaaagaggcc caggatg
<210>24
<211>304
<212>DNA
<213>Homo sapiens
<400>24
1 tttttgctat tcaaatgcac atttatttaa atgtttggaa ttaagagcaa cctagaaaca
61 gaaaacgtgt tcaaatattt attttaacag catcttttgg aacatgttta tttcccttaa
121 aaacgacaca gaggaaacat gtacactgta acaacacctt cccctctgtt tctccagaag
181 aaaaatgttt ctgcatgcct gataacagat ggtgcaacca acagtaaacc tggctctcta
241 caccagtgaa gaaccattct ccaaatgccc agtgtgcctc agaggaaata tacaatttaa
301 aagt
<210>25
<211>459
<212>DNA
<213>Homo sapiens
<400>25
1 tttggtttta taatgtatat attttaagta ggacaggttt tttttttttt aaacttttgg
61 atgttttata aatttgtcca atttagtcat ccaactgaca aagcaaagca accccgcgct
121 tgatccagtg ccgagtaggt tggtctgttt ttaacaacat tgcaatgaca aagttagtag
181 aatgtcccgt agtggaaaga gttcctttac gttcacttgt cttgtagagg ggacagacat
241 aggcatccga ctttataatc cgagattttt gagttggttt tatccatatg atgggcatca
301 ggtcaaacag aagtttggga tattgtcagc aagcaatcca ctttctcggt cccagcgtgc
361 gccatcgaga tacagtccgt ggatataaac accatcttct ggtgatgtgt cagatgtatc
421 agatgggata acctcaaatt catatcctag caaatcaat
<210>26
<211>459
<212>DNA
<213>Homo sapiens
<400>26
1 gcaggggatg gtgaggcact aacatcacag ggataattta tacaagatgg atgatattgt
61 agattcattt gcattgaagc caaatcctca atctcttctt catacatatt gctgctaaat
121 tgtattactc taattccaag ccagtactct tggctttttt gaaccataca ggaaggattt
181 agatttattc ctattactta tcaccctgtt agattcatca accatttgcc cctggccaag
241 gatgttttcc acttattgct gcagaatgct tctagtctct cccatgttta tgaaatccat
301 gatttgctct ttaggtactc taaatgctca caaaccatct gaataatact ttgggactga
361 taccaaactt tcagtctata atttacagca cacgtttccc tttaacttgc tcctgcttcc
421 cagaattcca cactgatcat aatcagtgat tactgatat
<210>27
<211>549
<212>DNA
<213>Homo sapiens
<400>27
1 tttatggcta atggtgacac actttgttaa tttaaaaaca cgcccttccc acatagtgcg
61 tgaggcatgt gcacattttc ctagaaggac atgaatagtg atgtggaggt acggtggagg
121 tcaggcatct acagggtcat tcgaggagga acagattcaa gctttcggac gatcagtgtt
181 ttgtaaatag cagcatcatc agatctaaga caacattgga cctggcaggg ccttttcttt
241 gggtggcatt aattactcca gattcagacc aggccatcct ctcccggccc aggatctgcc
301 tctccctcct ctgtccccac acctagacat gaaagccata acccttgggc ccctcagagg
361 gaaaacacaa ggggtgcgag gggtgggggg gcggcgatgg ggggcattgt tgagcagtcc
421 tctcacggct gggagcctct gctgcttcca gttaactgcc tttctggaaa gcgattctat
481 tacatgatgg aggcagcgga atgaaacatt gaattacacg gcacgagatc caagctagtt
541 gttctcatc
<210>28
<211>742
<212>DNA
<213>Homo sapiens
<400>28
1 ttttttaaca tgattgcctt ttatttatta tgcaggttgt tgatttacat agggagttgg
61 agatgctaac caagcatgga gttttcacat ggtctatttc tgctgagttc agggacttgg
121 agacagcctt taacttctgg caaaaagaca atttcacaaa ggtgtttaaa accatccttt
181 ggtttttgat cctgagtcag agacggacat gtgcttatga aagaaggtag agtttcaacc
241 cttaggtaac cttaaaagag caggaactat gttgtgtgta agtcatgtgc agtatacaaa
301 cttgatatta aatgacaaat tggaacaatc tttctctagg aatgcctctc tttcatagag
361 gcatcacagt gagtctctta aagccttgat ctaggtgtgt tacagatggg cttacagagt
421 atgaatgcac gataagaagg aaattggata gggagtgagg atatgaaatt taaaagaagg
481 aagaactgga acaaggcata ggcctctttc aatttttcgt ggtgtgcagc tgccaattct
541 tggacattca agaaacgctg gttgacatta tcttttttct tcacaatctg atcaacgttg
601 aaagtcccgc tggagagcaa atcttcagcc aatgtattca ggtccttgag tgcatnctct
661 cgagccaaca tctctctctt ncatagctga tgcttcttga gtaggtttct gctgaagcca
721 agtcctgggc tgatctttat gg
<210>29
<211>458
<212>DNA
<213>Homo sapiens
<400>29
1 ttttttttac catcactttt ttatttggca gtaatagtaa tacatccatg ttacagtgct
61 tattttggct aaagagccag tgcaattttt tctgctactg tagtgtttta cttttacaca
121 aattaatgag tacagtcctt acatttagga gacagaaaca tttactgaca tcagctaagt
181 aaagctaata catggttgtt cacaaactaa acaaaacaat aacataatat actcattagg
241 aacaaagtag ggatgttctt atttccacta atcacagatt atataaattc ttaaatttca
301 cttacatatt tctaaatcta agcaaaaaaa ttagaattca gcttaattcc tataatcatt
361 ttgaaattta catcaaataa tttctgaata tatattcaca ttaaaataaa agtaaatggg
421 ttatataaac ataatttaaa atttctgatt atgaaaag
<210>30
<211>542
<212>DNA
<213>Homo sapiens
<400>30
1 ttgagaaatt tttttattgt atttgttttc aagtgattct ggtaaccaaa gtattacagt
61 tacagggcaa gcaaacattt aagccaaaaa acaatcatat ggcaaaggcc tctgctttaa
121 tctactacat gagaggtcat tgttactcat ttccaaagct gttatagact tggaatgtgg
181 aattggagag gcttttcgaa tgtagttagt ggtgttcaca aaagaaccac tgcctttcaa
241 cttggaccct aagaatgaac ttatcacttc catttagaac ttatataagc aaaaatatct
301 gaaggcaaat atcaagccat gtttccacac tcccaaagta tctcatttgt catgagccat
361 gagtgtcact ttttaaactc cttccctgaa tgcccaatca acaggaaaag agcttgagag
421 caagggcaga agagtctaga ccaggtgttg ggaactatag ctcacagact ggccacttgt
481 ttttgtttgt ttgtttgttt gtttgttttt gagacagtct tactctgtcg cccaggctgg
541 ag
<210>31
<211>558
<212>DNA
<213>Homo sapiens
<400>31
1 gctttttttt tttttttttt ttctttatat aatttaatat ataccctatt aacaccagtg
61 tgcagatgta cagtactgac agtatactaa atagttagta ttaaaaatta ctttaaaaat
121 gttttcaaca ttaggtactg gtggaaccag agctgttatt ttgtacatca tatcatttat
181 gtgttgcaaa ggaaaagtaa aatttccagt agcattagaa aaaaatgtac tgatatttta
241 tgtaaatata caaaatctca tgacatctat tgaaaacctc acaattctat tgtgcactaa
301 taaaaacacc ttttcaaaca acttgcactt atactgagtt cataaaggct actataaaaa
361 tattgcaaaa gcaataaaaa cacaatatcg tacaaagtct tttgtctttt aaaagtcaaa
421 ttttaaaagg actgtattta tataaatgtg caanactttt tagtgaccct gactaacatt
481 tttgtttagg attatctgat tttagaaaac taagttaaat gtttttcctt attttctgtt
541 tcagaatgag aagaccta
<210>32
<211>528
<212>DNA
<213>Homo sapiens
<400>32
1 ttttttttac tttgagaagc catttatttt gcagccttca gtccaaaaaa agtcaacatt
61 ttcagaattt ttttatataa gttgtaggtc atttttataa caataaactt tctattatct
121 atttatctct cacatacatt tcatgtatcc tgagtattat gttacaacaa tctgctcttg
181 atagtaatgt tcctgataga ttaaaagatt gagaaatact tgaagaacga tcaaagatac
241 aatgagcatg gtatactttt gggttaaaat gtattctttg ataactgatg tcatatagat
301 ccctaagtaa atcaaatatt taatttccta catctgtcta ccttagttaa ctggcccatt
361 cctataagct taacaaaaac tcactttaca attatttaaa aaaaacattt aagagatttt
421 tagaagttaa atggaaagat acttttggac attacaatat ttttaatgag ttattatttt
481 aaaattagag atttcacata aaaaccagga ttctttgttt tctcttta
<210>33
<211>449
<212>DNA
<213>Homo sapiens
<400>33
1 aagatacagg gtctcaccct gtcgctcagt ctggaggtgt ggcatacagc cttgaactac
61 tggcctcaag ctagtctcct gctgcagcct cccaaagcac tgagattaca ggtgtcagcc
121 attgcacctg gcctttctct atccttcttt aagaatggta atttactgct ccagggaatt
181 ttgggaaaaa caggtgattt ttggtgagtt acggatcact gggaagagac cctaaacaca
241 tatacacgca tgcattcgag cacacacacg cacacatgtg catagacaaa taaaattagg
301 caaggagaca aaatattatt gaaaacaaag tacccacaca gacaataaca gatgcagcac
361 atctttgtgt ggctttccat gctaccaagt ccccaatggg gacttcacaa ctgctgatga
421 cacaagccca aggaagtgac atgccagat
<210>34
<211>536
<212>DNA
<213>Homo sapiens
<400>34
1 ttgcatgctg ctgcatggtt ttattcctca tgggtagatg aacacacact ggtatatggg
61 gaaatcctca cccgccctcc gctgctcttc tggaggtctg tcctcttgct ttcctcctct
121 gccccagcct ggtgctgccc caagccctgc cctggcaaag agagaactgt gcacagcggg
181 gaggctctcc aagccagagg cctcgcacgc agctgaggaa tgacgcaggg gcctgcaggc
241 agctcacgcg gagacaggtc tgtggggccc cgcgtcagaa cccacttcac atcccaaagc
301 cccacctgct ttgatcttca atgctgggct caatgtcaga tctggctgac cagaggggct
361 caatgaggct cactggctct tcatgcaccc tgctgtggcc tgagcacgtc cccccaaaac
421 ccacctgctt aagtcctcac ctgcaagggg atggtattag gtggggcatt tgggaggtca
481 tccggtcaca gggcaggggc acagcctcat catgggacca tcacccttac aaaaga
<210>35
<211>737
<212>DNA
<213>Homo sapiens
<400>35
1 aagaatcatc tgcttattta ttttacagag taatgattta aattgagtac atttgggcac
61 agtagatatt tgacacgaaa aatagttgta tcagaatgag catcagaaaa atagcaactc
121 atcttacaca taataactag aaaatattat tctgtctgat catttaagtg ttacaaatcc
181 tgagaatgtt atacattgaa caaattaggt actaccttat aattaaatct cgcttggaaa
241 gaatccttta aaaaatgctc tgaggtacaa atcacctatg tattaaacat gagtctctga
301 cattatacaa agagtttaaa caaatatatc aaacaattac aggccagtgg ctcttaaaac
361 agctatcagt caggttcaag gacacactgt tccacaattt cccgtaagtt ggggttttcc
421 attgcagctg ccactcgctc tttgtcattt atctgcttat tgatgtcttc ttctgttgag
481 tgggttcctt cagaaatgta gatttccaac ttatgtttaa atggtaaaca tcgctgaagt
541 tttactctta agcacagccc aataagagtc gccaaagagc aatgaggtac tggtggcgtg
601 aacctgataa taaccagata ttcttcttca tttattctcc tgaacttcca cacacctttt
661 ccgagaccac ttccagttct tcctaaagaa ttgggccttt ctggggcccg ggatagtcta
721 atcaaatcat aaacctc
<210>36
<211>582
<212>DNA
<213>Homo sapiens
<400>36
1 gatcaatacc acagtttatt tgtaaacatg ttatatgtgt caataatcaa attgacaaca
61 ctttatagat ttcattgtat aatattaaca tcctaacaga aaacgatcca ctgtactcag
121 ttacagtttg gtattttaaa atccttaaat acaaattgta tttgaaacac tgaacacaaa
181 aaagaaacat gaatggcaga gaaaactgaa aacaacaagt aaaagaaacc aatattccgc
241 tccccttgaa aaaaaacata aaatcatctg attacataat ttaaaaaaga aaccaaggga
301 attcagatga cattttttga tttaaagttg catttcttca aatccatttt tagaggtgaa
361 attgtatcaa tattaaaatt ctaatgtctt tttgngatat ttgttcttct ttcccctctg
421 gttcttaaaa gatggtgggc caggtttaaa ttactttaag ggaagggaaa aaagaaggga
481 ggaacttgga aaaagcccag gaaattctat tttcgcaaaa attgttggcg gaattttcga
541 gatctttgtg ggaaaaaaaa aggcgcttat taaaaacctt ct
<210>37
<211>451
<212>DNA
<213>Homo sapiens
<400>37
1 tccaatgaaa gtctatttat ttgctcgtaa tgagacagaa cgctacaatc tgttcaacac
61 tgggctggac actgcagtga ttaggggcag gtgtggggca gaaatagaaa tcacatgatc
121 gccactgatt cgcagtggag agggcgctga gctgggcccg gcaggcaggc agcctcagca
181 gagattcagg cagtcagcat ggtgcggccc tcccgccagc actgtcaggt cagcagaggt
241 tcaagcagtc agcatggcgc ggccgctagg accctctgcc atggctttgt gacactgcag
301 ctatgctggg cgggactccg ccccccaccc cacccctgca tcggagggca gggcaggcag
361 caagcagcat ttggaatgca gggtggagag gatgctagaa gtatgtgatg cacctgcggc
421 actgacagca tctctgccct cagaggctga g
<210>38
<211>532
<212>DNA
<213>Homo sapiens
<400>38
1 gattgggtaa aatatataca agataatgct atacttttaa aagagaaaaa gcactttaca
61 ttggttaggt ctttggatct gcatatgtcc ctgaggtaag tattccagcc atgggaaact
121 gaagcacaga gaggtagtca cccaccaaag ctcagggctg gcctccagag cttgaaccct
181 tgcctgagct ggggtcacca gcctgggtgg gccacggagc gatcatcatt tctccatctg
241 atcggaaagt cacccaccac tctgttctgg ggcccagccc gcctggctcc aggcaggctc
301 ggaggccgtg ctctcctgga cctggtgcat ctggctgcag ccagcactgg gcaggaaaat
361 accgctggag cagtgccagg tgcaggacgt ccccactacc tgcttcctcc tgggtctgga
421 gggccaccat gagcccacaa tcccggccag gcccaagccg gtggctgaag tgggcagctg
481 tgtctagaag taaggcaatg aggtaggcgg cttcctgggg ctctgggtct tc
<210>39
<211>334
<212>DNA
<213>Homo sapiens
<400>39
1 tttggtatag aaatgtttaa tacattaaat cttatgaggt aaaatacaaa aggtagtatt
61 cataaaagag ctattttgtt ttatttcact tgaagttctt aagagaaaaa cataagagga
121 gatatagaca gacctcatct gtaaactcat gttgctcttt acaaaggaag agacccagca
181 gcttttaagc cagttagtgg tagagacagg caagagtcac caaaaaaggt cattttagta
241 ttctgtttaa tggaacttag aaagagacta catcgtgggg ttgggggagg aggggggagg
301 gcantangga tntacctaat gtaaatgacg agtt
<210>40
<211>562
<212>DNA
<213>Homo sapiens
<400>40
1 tgcttaacaa gcagttttat tcctaacaga caaaaataag aagcaataac atatccataa
61 gcagatgaat tcgtatacag tgatttccat agcatgtcat attctaagat aaaaagaatg
121 aactactcat atatcatgat tgatgaatgt caaaatatgc atccctgcta tgtgaaataa
181 aaataataat aaagaaaaaa gaagaacatc taaataaata gggctcagga gagtactcag
241 gtatgaaaag aggaaacatg ttatatcagt catataaaat tccagaaaat tgacattaat
301 ctactgttaa gcaaatattg taattcagtg attgtctgga ggatggagag gccagagaga
361 aagatagtat ttatttttta agagacaggg tcttactatg ggacccaggc tagtctcaaa
421 cttcttacct caagcaatac tcccaccctg gactcccaaa gtgttgggat aacaggcatg
481 agtcaccatg cccagtctta ttacagcttt aaaaatttaa tttgaaagaa ctatctggat
541 ttaacattat ttttgacaaa ta
<210>41
<211>542
<212>DNA
<213>Homo sapiens
<400>41
1 gcggccgcgg gacgcgctgg ggccgggcag ccgggaagcg gggcgctgac ggtgagtggg
61 cctggccccg ggccggtggg tccccgcggg gcggccgggc gaggagagag gcagcttccc
121 ttcccggagc ccgcctgggg cgtggaggct cagcgtccct atcctccatc ccgccccggg
181 acggcggaac cgggcctcag ctggggacct caccaggggg cggggcctcc cgcggtcccc
241 ggcgctgctc ccaggactgg aggggcgtcc gcgcccggga gaggggttcc ccccgacagg
301 gccccgggcc gccacgaagt tagggaagcg ctctctccag ggtggaacct gggcccgcgg
361 ctcccgggag aggagctgcc cgcggaaggg gtcggngtgg gctccaggct gggagaagga
421 tctgactntg ctgacctcgc aggctggtag gggaagctgc cccccagagc agcatggatg
481 ccccgcgaag ggacantgga gttgctcagc aacagcctgg ctgcctacgc gcacatccgc
541 gg
<210>42
<211>561
<212>DNA
<213>Homo sapiens
<400>42
1 tttttgagcc aagaagtgca gtaggctgcg gggattttat ttctgccccc agaccaaggc
61 aacggagccc tcacctgcct gccaccccca cactgggccc aagcaagctc aggtgagcgc
121 acatacatcc accgtggcgg gctcctctcg ggtgaagcca tcaccgaagc cctcatcgtc
181 caccaggtcg ggctcacggc agcacatggg acagaggctg ttgcgcacag cagaggctcg
241 gagccagacg acaaggttcc aacgctcacc agtgcccaag ggccgggctc catgcagctg
301 gccgccacgg tggaggacac cctggcccac cacgtgctcc acctccaggg gctccgtcag
361 ggctgtgggt gcctggaaga ggcccccaaa atacagggcg ccccctgtga agaccttgcc
421 caaggccaca ttgagggtga gctcggcatt atcatagtgg cagcccagct ccaggtcctg
481 gcccggtgcg tatttgacca caaaggcccg gtggctgtcg agccggcccc cgccacagtc
541 agggtacagc agggccatca g
<210>43
<211>484
<212>DNA
<213>Homo sapiens
<400>43
1 tatgatccaa ttacaaaaaa gttatctgat tttatttctt atatatttgg cacaaacagc
61 agagactgaa agctactaca catgttgaac tgctgacagg aaaagaaccc agtagataaa
121 tctaggcttt ccactccata tattagctgg ggctgccata acaaaacatc atagactggg
181 tggcttaaac aacaatttac ttctcacagt tctgaagtct ggaagtctga gaacaggtcg
241 ccagcatggt taggttctcg tagggttctc ttcctgttct gtagacagct ggcttcttcc
301 tgcatcctca catggtgcag agagcaagca ggctttctga tgtctttttg agacgagtct
361 cgctctgtcg cccaggggtg agtacagntg gcggatcctg gctcactgca acctccgcct
421 cctggttcaa gcgattcttc tccttcagcc ctccgagtag ctgggactac aggcacatgc
481 cact
<210>44
<211>544
<212>DNA
<213>Homo sapiens
<400>44
1 tttaaaaaag attacaaaac tgaatttatt gagattcaca caagatgcac ttataaaatt
61 agtactgaat gccattaaaa cagaagaaat gaacataatc ccgaactccc aacagcatct
121 gcaaaggaat ggaaatcttc tgaaaatgac agcgcagtaa cagaataatt caagcggaac
181 tgaagatcta tccaaaccat gttcctgctc tgaaatcagg gttgtgtttg gcaaagcttt
241 ccccaaacta ttccattcac ggtggcattg ttccttttgt tggtaaaggg agacggaaaa
301 aataactcta aaatggggct ccattaaaac acacacaaag caattaaaag ctttttcatt
361 tttaacttaa aatacttcct ggtttcacaa aggcatgtcc atacaatttc ctcaacatta
421 atcttcagat ttctttagtt aatgcaaaac attaaaattc acttactttt ttgtatttca
481 aatgaacaag tcagaacatt aaagcttgct ttatatttca tccaagagac agacaaatca
541 ccta
<210>45
<211>587
<212>DNA
<213>Homo sapiens
<400>45
1 tttttttcac aggtttgtct tttaatcagc atttttgaaa attcattaaa aggtctgata
61 cgaaaagcac caggctaaca aatcagtcct gaattcaatt ttacaaaaac accctctcct
121 cttccatctc atccctaata ttcctacgtt atctcgaggt gcagagaaat ataaaaggga
181 tccttgacat aaaaacgtat taaaaagcca ccatgagtag aatgtacggt aacagcgtct
241 gactcggccg gcaggacagg aaacctacag aaccaccatg ccagggcttt gtgtgagcag
301 gcgggcactc cacatgggca cgtgtgtgag caggcaggca ctccacatgg gcacgtgtga
361 gcaggcgggc actccacatg ggcacgtgtg tgagcaggcg ggcactccac atgggcacgt
421 gtgagcaagc gggcactcca cattggcacg tgggagcagg cgggcactcc acatcggcat
481 gtgtgtgagc aggcgggcac tccacaatgg cacgtgtgag caggcgggca ctccatatgg
541 gcacgtgagc aggcgggcac tccacgtcgg cacgtgtgag caggcgg
<210>46
<211>535
<212>DNA
<213>Homo sapiens
<400>46
1 tgtagtggca agtgatgtat ttggcttaaa gaagcttgtg ttactgaagt taacgcagaa
61 gtctggcctc cgtttgtggg attattaaat atatatccta cgggatcaga gtcatctatt
121 agaaacagtg atagccctgg gtataaataa acatttcttt agagtttggc gtcagttttt
181 caatgtccat cctcaaatca gacagtctgt ttgaggggtc agttttcagg gtgcatctgc
241 accgagaggc ttgaggcagc aatgacccct gggctgcagt tcggttgcag gcgctggtgg
301 cgggcagtcc aggttggagg gtggagatgg gccaaggtca cagatgctgc agggcttcct
361 gtccttgctc tgtgtaaccg tcatcgcggg ggggggttgg gcacttgtcc aaagggaccc
421 ggggatgcgc acgttccttc tgtgtgtggg ccaggcgctt cttatctcgc aggctgccca
481 tctcctgctc cttggtctgc aggtctgcct ccagctccac cttctgttcg atcag
<210>47
<211>469
<212>DNA
<213>Homo sapiens
<400>47
1 ggcatgtcat cattttaatg atgtgatctt tggtgtttcc ctcattagct gtagactatc
61 ccctctcctc ccaccacaat gtttctatga tgagttacaa acagaaagga aatcacattt
121 tcatactaaa aacaaaatga tcagagcctt gatttctcca ctagaaacta cacgtacagt
181 taagagtcca catgcaacac cttaaatcac agactgagac ctcacattct gacctggagt
241 ctcctcccct tccccagcct tgggctagct ttggcctagg ctcaggtaat actgacaccc
301 acaggcgctg ctctgagggc cttgcgggga gaagactggt ggacaagccc tgggggctgg
361 ccagcctaca ccccccactc ctgagtgagg acctgcccac tgtcccttga ccccatcctg
421 tatcaggcaa gcaggcgccc ccttacccca cagtantccc ccattagta
<210>48
<211>427
<212>DNA
<213>Homo sapiens
<400>48
1 tttttttttt tttttttttt tttttttttt aaacagggtt tttaattgaa aataaagcta
61 aagcatgctt ttcaacaggg caaatttcca taattttgat ttactggttc catcatcaca
121 acacagtaca ccagcatggc ccttacccag ggctagaccc tagcatttgc atgttgaact
181 actttgattt taagggcaaa tataaatagt cacctctgat tgtcacttgc agtaaaaact
241 ctcctgcttt atacacagga tggtctgaaa acacaagttt tagagagatg caaaaaaagt
301 ttaaggtcat aaaatccaag ggcctgagtt ctggtcccaa aaagccctga aaaaactggt
361 atttttcatt tggggtattg caactctgac ccctcatttt ctttttcttg gggggattta
421 ctgattt
<210>49
<211>508
<212>DNA
<213>Homo sapiens
<400>49
1 tttttaatag tgtcttgctg ttactcagac tgaagtttcg ccatcatagc tcactatagc
61 ctcaaactcc tgggctcaag ctatcctcct tcttcaccct cccccaccat gctcggctaa
121 tttttaaaat ttttagtaga aatgaggtct tgctgttttc caggctcgtc tcaaactccc
181 aatctcatgc aatcctcctg cctcagcctc ccaaagtgct gggattacag gtgtgagcca
241 ctgtacccag ctgagagctt aagtttcttg cataaaactc agctgtatgc tgaagtgttt
301 agaagtgaaa tgtaatgatg tctgcaactt actttgaaat acattaataa aatggataaa
361 tgaatggata gagggatgga taggcagatt gacatgtgta aaacaaatag agaaaaatgt
421 tatttgtaga atctaggtga tgctaaaggc cacacagcca gtggggactg agccaagcta
481 ggaatccagg tccaccaatt caggagag
<210>50
<211>507
<212>DNA
<213>Homo sapiens
<400>50
1 ttttttagaa aagtcctttt tatttcttta aatcagaggc cccctctagc gtttgaaagc
61 agtcacaggc aacacacaat gcaattgaaa gtaaacagtc acagcatttt accgcagacc
121 ttccaaaaca taaggcttgc ttctgttcca ttcaagtggc acaaaaatca atgagagaaa
181 aatagctaac cataattaat attgcaaatt cctgctatga ttcagattca ttatcctctc
241 attcagtaat ttctttatta tttactgctg ggttattttt tactagatta atactctgtt
301 aataaggaaa aaatatataa taaatatagg gactttaatt tccctaataa gaaatatacc
361 tcatttgaaa tgaccgaaaa ttaatgttac catatatcct actctgcttt gatggaagtt
421 attggtaact tcctaaagta tatacttaat tttaacttta ttatatttgg aaatcagaac
481 tttttaaagt acatatattt tgaaatt
<210>51
<211>541
<212>DNA
<213>Homo sapiens
<400>51
1 tttttttttt taaaagaaat ggatgctttt attcaacctg agttaaaacc acaatgaatc
61 atatcagagt tcatgggaat cataataaat gactagtaaa ccactcacaa aatcaaagct
121 atcaacatac taggatcaca cacattaatt agtgctatag ttcaaaacat accctttaac
181 ttatttagca cgcaaaataa tcataaaagt aatgtctata agctctaacc aaaaatttta
241 aaaattaaaa atagcacaat tctacaattc tgattttacc aagaaaataa accttttttg
301 gcacatatta tcctatgaaa atggaaagct gagtcaggct gctctgcttt tcacagcaca
361 aataagcatt catgctatca gacttgggaa attaactcgg tgacaaaaat tcactggaaa
421 atagaatcct tggaaaaatg gggtcaggtg ccatccactg agaggcaatg ataatgtgtg
481 tccttcgtta ttagcacaaa gttaggcagc acactataat tntagctaca tgcaactcta
541 t
<210>52
<211>369
<212>DNA
<213>Homo sapiens
<400>52
1 tttttttttt tttttttttt tttttttttt tttttggcta aaatttacca catttattcc
61 aaattgggat tttataacaa ggagaagtct ttaaaatact aagggggcaa aaaaatacat
121 aatgggagac tgacatattt tcaaacttaa gtcacattgg catgaatcaa aacaaagtta
181 ccccaactaa aatacatata agaaagagcc actgaaggaa aagcatcaca agacacagca
241 acacacttca gttcatttgg aaaatctaaa attaagggtc aaagcagctc acttaattga
301 atctgatttt caaaatacac atttcaggga caagcatttc cacttacctg cttcattatg
361 ctcttaaaa
<210>53
<211>298
<212>DNA
<213>Homo sapiens
<400>53
1 tttttttttt tttttttttt gggtttgacc catttttata gaaagccagc taccaaaagt
61 aatgagtccc cagggccaac cacattggag gcctgggtga gggaccaccc agcagggagc
121 aagggaggga tgcagacaaa ggtagcagag accctggcaa cctccttcca gtactcagca
181 gtcacaggtt aactgtggga gaacccagca acacactggg tccccagcac agtcttgggt
241 gggcccttca tggaaggggc agggaggctc tggatgcttc tgcagtcatg ctgaccca
<210>54
<211>520
<212>DNA
<213>Homo sapiens
<400>54
1 ttgtttttta gtataaagag tagtatttta ttgaataaga tttgctcaca gaaaaataag
61 cttacatcta caatgaatgc cagaccctac agcagaaagc cattttctca cttttccaca
121 cacaatggtt cctactaagt gaaaaaaaaa gccataaaat ttcattcaca aatgtactac
181 tctgtctcaa aacatcgcac ataatcatgc actgtactaa agccattaga tcagttcttc
241 agtcaggtta aagaagtatc cctctaataa ctgactttta taatgctatc aatatccact
301 cccaatcagt ctgccattgt taatggtgta cagcattact gaatacaatg gaattgatga
361 cgcccatatc cacggacaaa ccgtgactta tgatggtttg atttacgatt tttcaacgtt
421 atgatgggtt tactggaata ttagatgcgt ttctgagtta cactgggttt atgagtatgc
481 gaccctatac tccaganaca gctgtagaag gaaaaacagg
<210>55
<211>166
<212>DNA
<213>Homo sapiens
<400>55
1 tttttttttt ttttttcagc agcacaaatc ccttttatta accatcttcc ctttctcgag
61 tatacacaag tagaaaagaa acagggaagt accccgatag ggagggtggg gagaggatgg
121 gagggggctc ctgaggttag gacaagaggc cagattccag ctcctt
<210>56
<211>462
<212>DNA
<213>Homo sapiens
<400>56
1 tttggttttt gtgatgctgg aagaatgacc agaatgagtc ataggaagag tatacattcc
61 tgtagtattt tccaggacag aggtgtgacc tcctagagta ctgggaccaa aattcccaag
121 tgtctgcaac cttgctttaa cagtatggga gatcacctct atcacctgga attcccctgg
181 aactctggaa tatacaagag aagtatgaga cttgggtctt cccttggctg tgtttaattc
241 actcttctat ggaataccaa tgattctcac taagactggc cttttcataa gcacaatgtg
301 cattttatgg agaagatttt acactttgct ctatttagaa agaataaata tgagcagtgg
361 tttaggtttt atgccctgga cttaatatgt ttctgattcc tgttttgaga ttaaattctc
421 atgtaaatag aaaaatactt attatttctc ataaggccaa gt
<210>57
<211>424
<212>DNA
<213>Homo sapiens
<400>57
1 tatgaacaga gtggtttaat gaaactactt tttttttgtt tttgtttttg ctttcctttt
61 cccataaatg ccatcgtttt ggtaaatgtt attactacag cctttttttt tttagttctt
121 tttttgagaa ctaccattta ttgagaatgt cttattccag gttcctacat gggttttcat
181 aactactgtt ttcaacaact ttgagacaga cataataaat aatgtagtaa acagcatgga
241 tataccctca gatctctaaa acccatggtt ttggcacaat gccattccaa cctctgccat
301 cttcatcctc ctgctttaac caggaaaaca tatttactgg gcagtagaca tgtcaaaggt
361 gaaggtattc atatccaaat aatactgtgc tgttgttttc aaagcaatcc atgtttcacg
421 agcc
<210>58
<211>515
<212>DNA
<213>Homo sapiens
<400>58
1 tttttttttt tttttagaag ttatgattaa attttattgg ggcataaaac ttgtgataca
61 ttgtaaataa aacagtagca aaacataatg ccagttgtga aacgtctgtg tatacgtgca
121 catgctattc tggttctttg gtttggtttg gttttttttt tgaggcagag tctcaaaaaa
181 aaaaaaaaaa cgaaacaaaa ctccagtgtc ttaatgtcct ggcgtgccat ctcctggtca
241 actggtaaag cagcaatcct ttcctataag ccagtggccc tttggagaag ggcacactga
301 acacaataag catgactttt agttttgtat ttttctccac ctctccctga tgctaattca
361 tacaatatta aatttcaaaa ttcagcattc ttgttcttcc agggagcatg aataggaatg
421 aaccacacct aatgaagtag gccctatcat tttattgatt ccttcccaaa gatcataatg
481 aaaggcctgt aagaaaagca taaaatcaac agatc
<210>59
<211>604
<212>DNA
<213>Homo sapiens
<400>59
1 tcagataatc aaaataattt ttatttggtt gtagaatgtt gtagtacata aagcaaaatg
61 tgtctgcagg atactccaaa tttaccaact tcaaacaaaa taatgcacag tgaagaatta
121 gaaaattatc aaccttactc aatacatgga agtattcctt aatcttcatc attttcatta
181 aacaaaataa tattttggat gagttcaaca tttctatcct atgctttatg tttagtgcct
241 tccctgaatt ggaaaaaatg aggtgtttta tttaaattta acactggtct aagggagata
301 tagaattaaa ctaaacaaga atcggttatc acaacctcac aacatatatc aaagtattga
361 gattgttggt ttactgactg tcacttttca ctggatatta aatatcctga gggctacaac
421 tgtatttttt aacctgtccc agtacctcaa caaaattttc actgaatgta tgaataaatt
481 atgttaacaa aaagttaaca ataaaagtat aatgaagatc cctttaatta attntaaaat
541 ctgaaaggaa aactggagtc acaaaacata tgttcaaatc ttaattcatc acaaattttg
601 tgat
<210>60
<211>792
<212>DNA
<213>Homo sapiens
<400>60
1 tttttttttt tgtttgtaaa aatcggcatg ctcctttatt tgttcagaag agcagccagc
61 atcaccctcg ccactcaaac ctggcacata cgcttcggag acaatggcct cgggaccctc
121 atgctgctgg gcccaggaga gacagttctg aggcagaaac tcggcgtcca aggggggccg
181 cgggtcaggc actgtggtga agggaacgcc ggggagtccg gccccacctt gcagctgggg
241 acacgggggc gcaaacagag ggggcaggct agtgtcccgc tgccccagga acagacctca
301 gggccccagg agggtctgca ggcagctagg agcctgccaa gtgctggtgg aagtagaggc
361 cgaaaaggct ggcgagcagc tggcaggcag ccgtccacca gatgaggtag gccaggacgc
421 cacggaggaa gaggggagta agcaggccac ccagggcccc ggcaatccgc gctgcagtct
481 gctggacttc gtcctcccca gagccgatgg gggcagcgct ggctgaggat gggtcggggg
541 atagtagagg agtgggccca ggccccagga atagcctccc agcgtcttga gagagtgtgc
601 gtttgaggtg agatgagcgc gtcagtactg cagctcacca ggtcacatag agagactcga
661 ccacctctgc tgattcacgg gctcatgcgc cagcctctcg tccgaatctt gcctgaggca
721 aatcccctat gaagcgataa atcgaatcag ncaagtggnt ccgtggaatg tatcgtacat
781 tccccacata ca
<210>61
<211>558
<212>DNA
<213>Homo sapiens
<400>61
1 tttttttttg gttacaggtt ttactgtctt acagtgtgaa taccccatga aatcgtagaa
61 ctctttgcaa cttatgtcac acctgtatct tcaagggcaa ggattatttg ttgctatagt
121 ttttatgtca ctaaagtacc ttttagtgtc atatacatag tagataacac tggataataa
181 taataaaaaa gactaagaat gctaaagtag tctaggaatt cctaaatctt aataaaaact
241 gagaaataaa gttgtgatac tatataacac ttaaggcacg agtaattttt ttttcaccgt
301 ttcccctcca tggctatgaa ggaactggga gaaggatgct cctttcatta attaaaattc
361 ttcactttga aaatttaagt ttattaggga aggaaggaag aaaaatatga agatgtaagt
421 atacatacct tggagtaagt atttccttat attggagttt ctctaattta gctggggcta
481 ctaatgacat gggaatcaca atgttatcta tgtcataaga gctctcactt ctcaatctcc
541 gtcgtgcagt attctgca
<210>62
<211>478
<212>DNA
<213>Homo sapiens
<400>62
1 ttatgcaatg ccttgcgttt atttcggaga cgtgattggt gcctagactt ctaaagtaac
61 agaatttgat atggagtgtt gaaagctgaa ctttatcaat tatcacagca aaaatctaag
121 taagcgcccc gtgagaaccc taaaattaat aaaagctatc ttgtatgggg cacttacagt
181 tccacaggca cttgtaggcg ctttattcac aataatcgca gaagagcagt ataaagagca
241 tgcactctgt taccaaacca catccctgct ctacaggtgc tcccttagcc catctgtgcc
301 tcaattccct aagccgtaaa acggaggcct tgttctgacg ctgtgataat ttagtgcggt
361 aataggaatg atgcactgaa cacaaccctg gagcatagta actgattaat attagttaga
421 atattcatta tgatgataat gattggcaga gccctcgctc ttcacatgta ttcccaaa
<210>63
<211>211
<212>DNA
<213>Homo sapiens
<400>63
1 tttttttttt tttttttttt tttagatagg aattgggttg caaaaagccc cttttgttta
61 ttttattttg gaaaaaaatt ttattgggaa aattcacata tatatataaa aaattcaatc
121 aatacaaaag gatagacaat gaacaaatga attccccttc cactccagat ccccacccca
181 gatccagacc tcctgagccc actttcccca t
实施例1
人肝脏组织的mRNA的分离
组织分离(Tissue isolation)：肝脏来源于5个成年男性，在肝脏切除手术后， 将肝脏组织立即置于液氮中冷冻保存。
mRNA的分离(mRNA isolation)：取出肝脏组织，用研钵研碎，加入盛有裂解液 的50ml管，充分振荡后，再移入玻璃匀浆器内，匀浆后移至50ml新管，抽提总RNA (TRIzol Reagents，Gibco，NY，USA)。用甲醛变性胶电泳鉴定总RNA质量。用带Oligo d(T)的纤维素柱分离总RNA中的mRNA，定量。
实施例2
cDNA文库的构建(Constuction of cDNA library)
以mRNA为模板，合成双链cDNA。补平末端后，加含EcoRI切点的接头。磷酸化 EcoRI末端后，用XhoI限制性内切酶消化1.5小时，再进行片断分离。过柱筛选长 度＞500bp的片段，用酚-氯仿抽提，乙醇沉淀，无菌水溶解，连接至Uni-ZAP XR载体 (Strategene，CA9203，USA)，以ZAP-cDNA Gigapack III Gold Cloning Kit (Strategene，CA9203，USA)进行包装，宿主菌使用XL 1 Blue MRF’ (Strategene，CA9203，USA)细菌。涂板并测定滴度。
实施例3
测序及数据库建立(Seqencing and Database Constructing)
挑选文库中有外源片段插入的克隆，扩增后抽提质粒(Qiagen Germany)，用T3 和T7作为3’和5’端的通用引物，采用终止物荧光标记 (Big-Dye，Perkin-Elmer，USA)的方法，在ABI 377测序仪(Perkin-Elmer，USA)上 进行EST大规模测序。测序结果用FACTURA软件去除载体序列，传输到SUN Ultra 450 Server上进行下一步的处理。所有的序列信息再用GCG软件包(Wisconsin group，USA) 中的BLAST和FASTA软件搜索已有的数据库(Genebank+EMBL)，将无同源性或同源性 低于95％的序列视为新基因建立数据库。
实施例4
基因的全长克隆(Cloning of Full-length cDNA)
在得到的新基因片段序列信息基础上，进行cDNA全长克隆，分两阶段进行：
(1)“电子克隆”(Electronic Cloning)
以新基因片段序列作为探针搜寻dbEST数据库，将重叠序列＞50bp，同源性在98％ 以上的表达序列标签(Expressed Sequence Tag，简称“EST”)序列认为同一序列 (Consensus Sequence)，取出并用AUTOASSEMBLER软件进行连接，部分EST可以延 伸探针序列。再用STRIDER软件分析被延伸的序列是否具有完整的开放阅读框架(Open Reading Frame，ORF)，用BLAST搜寻Genbank或SwissProt以确定该序列的核苷酸 和氨基酸水平上是否与其他物种有同源性，以帮助判别所得到的基因全长完整性如 何。通过电子克隆的方法，通常可获取人肝脏相关基因的全长序列。
(2)cDNA末端快速扩增(Rapid Amplification of cDNA Ends，RACE)
如果通过“电子克隆”方法仍未得到完整的cDNA全长，则在已有序列5’或3’ 端设计引物，在人类肝脏Marathon-Ready cDNA文库(Clontech Lab，Inc，USA)中 进行长距离PCR反应。然后对PCR产物克隆、测序。用AUTOASSEMBLER及STRIDER软 件分析被延长的序列有无完整的ORF，如无，重复上述过程直至获得全长。
(3)RT-PCR
对于5’和3’端的已知的序列，如果中间有一段间隙(gap)无法从已有的公共 数据库或自身数据库获得，可考虑采用RT-PCR的方法。在序列5’端设计引物，3’ 端引物采用Oligo-dT，在肝脏总RNA库中进行扩增。然后对产物进行克隆、测序。最 后拼接便获得全长。
通过组合使用上述3种方法，可获得人肝脏相关蛋白的全长编码序列。
序列表
<110>上海人类基因组研究中心
<120>人类肝脏中表达的表达序列标签G组
<130>NP-10043
<160>63
<210>1
<211>566
<212>DNA
<213>Homo sapiens
<400>1
1 tgtcgtagag ctttttattt tgcatctgga gtgtatttgg acacgtgggc tgggtggggt
61 aacaggataa tgagcagttt gggaaagagg caggctccaa gcaggctggt tcactcactt
121 ggcagtcact gggcggctat tcttgtgcct ggatgtgtta caatgcaggg gacacagtgg
181 tgagcaaggc tgctccaagc cctggctggg atgggggatg cacagacagg ctgatcaggg
241 ctgggatggt ggacacgcat cctctttacc ctgatcactg gttagaaata ctgcactgtg
301 gccgggctca gtcggctcac acctgtaatc ccagcacttt gggaggccga ggcgggttgg
361 attactcaag gtcaggagtt cgagaccagc ctggccaaca tggcaaagcc ccgtctctac
421 taaaaataca aaaaattagc caggcatggt ggtgcatgcc tgtaatccca gttacttggg
481 ggggctgagg caggagaatc acttgaacct gggaagtgga ggctggagtg agctgagatc
541 acgccaccgc actccagctt ggacga
<210>2
<211>492
<212>DNA
<213>Homo sapiens
<400>2
1 gcatgtacaa agctattggt ttattcagct gccaantggt ggaaaggctt cctttcgaag
61 tgaatagcaa aaatgcattc atgagcaagg cacttttata cacacacaga gagaaaaagg
121 aacgtattat gaacacatga acataaacat attttaatat tcagacctct tccctacctc
181 ctcccacctc cacttaaaca aacaaacana aaaaaaggaa acagtttcct gtaacatttt
241 agaaaccagt ttcacagaat acctacatag gaacaatttc tgatacattc agttacacta
301 acnntatgat atggtttcag gtcaataatt tccactaaca aatactttta ttctacaatt
361 ccctctnttt ttccttttag acaagacaac ttnaggctca gcngaaatat ttcactaggt
421 taataaggca ttcttctctc ttcttaaagt tgctatatat ttacncaatt ggtgnttcct
481 aacctttttt tt
<210>3
<211>683
<212>DNA
<213>Homo sapiens
<400>3
1 gttgttgcca attgtttttg ttgtcttcca gaggtaatac aaggctttgc tcctttagca
61 ggattcccag ttggaccctc tccagagagg attcatattt gaattcccat ctgaatacca
121 acccaaatgt tgatacagaa cactcctgta ttaaaattaa tatccatccc agataaacct
181 actctgtgac taagacaatt gagatcttct aggtgaagat gctataattc aaaatattac
241 atggaaaacc atgtcttact taaaacgggt acttgttttc cggccataat tattccagtc
301 tcttccacag aactgcttct gcaaacagtt tttttaatgt atcaaagaga gtctctcgcc
361 aacatttaat acagtcaaat ctattccaac ttcagagttc ttatatgtct tatttagcag
421 acactatgat tctatcttct tattctctgg aaatccatca gatgtgtgtt ccaacacaga
481 agtgccttcc ttccttctca tggtggcaaa agcatatcca tcatccccaa ggttntaaca
541 atcccttggt aatcaactct tttctacaag ccctgaagag taaacnttta tcactacagg
601 gctgaccatt caggggaatt nccataattt tcagcgctga taacaggnaa ctgcggaaat
661 ccccggcgat ttgccgggna atc
<210>4
<211>592
<212>DNA
<213>Homo sapiens
<400>4
1 gcactaatgc tgcttttaat ggcggcgcaa tagagaagta caaggtttta ctagcttgag
61 ctactagaag gtgtaaaaga tgcccgctga cactaccaag tagcctctta atagaaatgt
121 agaaaatata caggacaggg tggagatgag ctcttgaatt tagtagggtg aaagagttaa
181 ctgccaaccc ggcactatca ttgtacttgt ttggagaaaa tctgttttgt tctggtgatg
241 atggttttat cttccctttt agttgggtgg gaagtaacag aaaatttgtt tccccaaaag
301 tttgacattt tcttgttaat acacgtttca tttcagtacg atgccaactg ttaagcctgc
361 aggaatcctc ctgggtattt ctgaattgtg ctgtgtgcat gtgtgtttga aagctcaaac
421 agcttgtctt cttacagcat cgagttgttt gctttattgt tagacacaat attagcagtg
481 tatcctttcc gtattcccta ccatcgaacc cattaagact aaaatccttg tcaagtactg
541 gcttaaaagt tgggagatgg tggtttanaa aaaaaaagcc ttgcaaaacc at
<210>5
<211>664
<212>DNA
<213>Homo sapiens
<400>5
1 aaatggtatt catttttatt tcaacatgtc aactgtgcat ttccaaaaca gcaggctttt
61 caaaggaata aatcagaact gtaaacacaa gatacagtac aagtttttga cttcctacag
121 tcagtttcac aaatccacat actgtacatt cataggtgag gttaagcctg tcacccattt
181 ctttatttct ataattacac aagcataata aatacatctg cttttaaagg tcacttaaaa
241 tgagtcataa tttacagtac agtacgtttc agttcaagtg caaaaaataa ctatttgctg
301 aattctattt ctttcagtta ttttattttt aagctgtgtt ttattgtgaa gcgagacatc
361 caagtgtaga atttcttatc ccaaatgcag tattgggcag aactatggtg tctggagaag
421 ccctcacttt acaattgatc cgtctaatcg ggtcagaggg taaggtgagt tgctcattcg
481 actgttcttt cttcctctct ctccgtgctc atccacagct attataaatt atataattca
541 ctttattcag ctaaaacagt ttccctttcc acactncccc caggtttccc gttaaccctg
601 cttgatttta gtggcagctt acgtggatca nccaccatgt ctggngcatg gacggtgcgg
661 tgca
<210>6
<211>449
<212>DNA
<213>Homo sapiens
<400>6
1 tacatttggt tatgtgttta attacaagtg tttcaatagc tataaacatt gtaaaattca
61 gtatcaagtg aaatatttgg agagccccta tggcaacttg gaaaaaccct gggattctga
121 ggaacaggga gtagcgcacc agtcccagta aaatccagaa ctttctagaa ggagataaat
181 gcaggatgac tgcctgcttg ccctgggcca gctcagccag aaaaccatgt ccctgaaaga
241 tgggaaactg gttccttgat acagcagaaa aacactgatt ctaccccaaa tgtactcaca
301 agacagaaga acgtgcatat gtcaaggagg aatgttcaat ggctgggcac caggcttaaa
361 atatccgctt gacccagtgg cttaaaagca gttccttctg aattgaacag agaatccctg
421 cggagatcaa aggagcctca gctcgtttn
<210>7
<211>428
<212>DNA
<213>Homo sapiens
<400>7
1 gaggtggttt atgtgcccca tagccaagag aggtgngcac caaaggagga tatcatcaaa
61 tctgacaatc tggaaagcct ttgaaactgt tcttttccta agcacagtat tcagctgtgt
121 cctcttgaac ccatatctat caggtcaaca gctttagccc attccacatg atattggctg
181 tgggtttgtc atatatagct cttattattt tgagaaacgt tcnatcaata cctagtttat
241 tgagagtttt tagcatgaag ggcttttgaa ttttgtcgac ggccttttct gcatcnattg
301 agataatcaa gcggtttttg tcattggttc tgtttctgtg atggattatg tttantgatt
361 tgcatatgnt ggaaacagcc ttgcatccca gggatgaagc ccancctcgt gccgaattcc
421 tgggcctc
<210>8
<211>332
<212>DNA
<213>Homo sapiens
<400>8
1 tttatcagtt gaaatacttt atttaactta ttttaaaaat tgattcaaaa ctttttatct
61 tctttattag gtattctacc cttagattta acactcattt ttcccgagta tattttctct
121 tcaatataca aaggaatata ttttcataga gtgtgttgaa tattaggaaa atcaagagtt
181 ctggttctgt caggtgcagt atttagagtg ttattttgat aaaatccagt tgctctgata
241 tggcttgaaa atacttgctc ctttggagta taattgcagg ggaaaaactg tggaataggt
301 agaaattcac cataatgact tgaattggtt tt
<210>9
<211>428
<212>DNA
<213>Homo sapiens
<400>9
1 tttcaggcaa ctgcttctct tttattatga atatgatttg aatctaccca ctgcacactg
61 aggctactgc ctctctttat aaaaaggaag gaagaaattt ccgtttcccg cagatgtgat
121 gtaggaagct acaactgcca actcctgagc atgagggagg ggacaggaga atcactgagg
181 ccctgacccc aaggcaaccg agacttagct gtgtgcccac agctgccctc gccaggcttg
241 ttttgtgagg gaataatcct tagtgcttaa tccattactg ggggagtttt gctattgtta
301 agaaatgtca gcattaaaaa actaggagac ttcacataaa aatacaggga tgtggatccc
361 ctgtactcta tctagggtaa gcctgcgcaa cagtggacta acaatgggga tcccagtgga
421 gagagggc
<210>10
<211>304
<212>DNA
<213>Homo sapiens
<400>10
1 gagagcttag atagagtgta tttttattct tttttttcca tttccttttc tcttttcatc
61 ttttttcttt ctttctttct ttcctttttt tcctttgtat ttttatctta acatgcctag
121 acagagaaga acactggatt gtacataatg ggctttcttt gactctcttt tccagagtgg
181 aaataggatt ctgtgctcca tcattctgag ccatcaccag gggccgtcca taatcaacta
241 tgatgatgag agtgggaaga catgtgtaca tatcgcagcg gcaggtgctt cagcgatatt
301 attc
<210>11
<211>487
<212>DNA
<213>Homo sapiens
<400>11
1 taaaacttcg aaatatttat tattaagata taaatacata ttttaaaaat gtacaagtta
61 aaaatacctt caaagttaaa tgtcagttct ttcacaatca ttgtgccggg gtaatacgta
121 caatatcttg gcttactagt aattttatac cagtcacttg aaacaacatc cttaaacttt
181 aataatatgt tgtaataatt ccatttttca ctttttagag tttgatagaa gacagtcaat
241 atcgtgaagg atttcagagg tttttttcaa tgctattgat actctatttt cattaacttc
301 tcctagcaag gtttctgaag atcccgggtt tataatgatc aaaagactgt tgttcatttt
361 tcttgttaga aatctggttt aggataccac tattttctag tagcaagtta aacttctgta
421 tcttttcaag ttcagaagca acagaccaat tttcttggag aacagaagat gacgaatgag
481 tgggcat
<210>12
<211>591
<212>DNA
<213>Homo sapiens
<400>12
1 tttaggctga tcatactaga gtatatttat taaatttcac cattatgatc actttgttat
61 aactaaatta accctgacat aaactttgac attttaaaaa cagagaacat actgattttt
121 caactatttt acttacatgt gttttttaat tctcatgcag tgctcataat taggagatac
181 tacatattca ccctcacatt atgcttatta agaaccaagc tcagagggta gaaaagtgcc
241 aacttcattt tccataaact tcccatacat atattaagcc actgttcttc taattatata
301 cttatggtac attcttctgt aaaatgtata actgtagtca cataaccatt gttatgacaa
361 ggcggcaaca tcatttcaga ttacaacaaa tgagaccaaa agttgaagag accacagcat
421 ataaaagtca tgagaacacc acacacattt caatattttc cactttccag tcaaaaaaac
481 atgcacaaag ttttcttagc agatagttgt cagaagtgca taaaaaaact ggctttcagg
541 tcatatatgt tgagtgagga tcaatgccat tgcgtgaaga gagctgtgtt c
<210>13
<211>755
<212>DNA
<213>Homo sapiens
<400>13
1 gcaccaaagt gacttgctta ttttttttat taaatgatac attgaaagta taaaaagttg
61 ccgcagtaca aacaaattac agaataatgc atataaaagc gctatggact acacgtgaca
121 attctgctta acttccatct tgatgatttt atatcagtat ccaaaattat atactccagc
181 attttttccg aatatagtct tacatgattg caattcctga ggtaaactaa gtgtccactg
241 ccaacatcct tggacgaaaa ccaagtcccc cgggagaatt cagatattta tattcacaca
301 tacaatacaa cggaattcat caatgtaatc agatattgca gatttaaaaa tggaaaatgg
361 actcttgcaa tacttctgca tccatatata attttaccag cagggcctcc cagaatggtg
421 tgttagttta aatggatttc agtaataggt ttctgcattt agatagacat tcttggctaa
481 tctgnntaga gtgacactga ctagcattgc gaacagaaat caaagttttc acatttgcaa
541 gcagcacaga ttgtccctta aagaggagca atttgctact acagtcctac catcttctca
601 tgaaaacttt agcaaaggtg tcatgttagg ttgcggcaaa ttaattcgaa atccacgact
661 ttccatcagg gcgtcacaca agtcgtgttc cagaagagcg tgtcctcata aggctcttaa
721 attcgagtgc gatggagcca tctattcgtc cagaa
<210>14
<211>555
<212>DNA
<213>Homo sapiens
<400>14
1 tttaccataa ttgacttttt atttaaaaaa ttacacggag caatttccag cttatctttt
61 tttataaaag tactgcctat atcaaacatt ttatatcacg ttaattccat tgaagagctg
121 cctttttctg ttaaggtact gattccaatt gatgggatac atgcccttaa tacagaaagt
181 ttccattatt tattcaaata tcaaaattaa gattattgag aagtttattg ctttatggct
241 gggcaagatg ctactagcac attttaggta aataatattc tttattaaaa actatgaggt
301 cattctgttt aaaactttca agataattca cggaaaacag atatatctat tcaaattaaa
361 cattaaaggg ccaggctact agctatagac atttatatgt gaagcagctc tttttttttc
421 ttttctgaga tcgagtctca ctctgtcgcc cagggtggag tgcagtgggc caatctcagc
481 tcactgcaac ctccggctcc aggggttcag caattctcct gcctgggccc ccaagtagct
541 cggaatacag gcgcg
<210>15
<211>476
<212>DNA
<213>Homo sapiens
<400>15
1 tttttttttc acggacagac agggtcgttt gtcacagcag agaagcacct cacggctcct
61 acccgcactc atcgcggaca gtgcctgcag cgggacgacg cagagcaccc tccccagatg
121 aaaacaccag caccaggagg tgggccgtag ccagagctga gggaggaggc tgggggctgg
181 ggctcagggc cccccccggc cacagcgcca ccctgagtgg ccctgaaaat agtgcacagt
241 gctgggtact gccccggccg gacgacacta gttgttgagc attccggcca caggcacacc
301 gctggccctt ccttggtgtg gcacgagacc acgggcactt gcaggagctc cctgcatgct
361 gttttgtgct ttggtctcag ggagcaccct cctacctcgg ggtcccagag tgggcagccg
421 ggcaggtgtg aacagtgtga caagggtacc gtggggcacc tggtagtgca cccaga
<210>16
<211>390
<212>DNA
<213>Homo sapiens
<400>16
1 ttatattcgc ggccgggcgg cgtgtttttt tttttttgct gcatatatag ctgtggtaca
61 ttttaatgtg aaatgatgac tgcatcagct tatatccatg gagcagattt tagcattcag
121 cttgggtctc ccagtcaata tctacgagtc tcttcttaag gagatcgatg acacagatac
181 atacagacta acaaatgtga taccaataat caagaattca ctcagttaag attttgccca
241 ctgatttcca cacaagaaac ctagaattta ctagattctt gtgcctgtga ggctccactc
301 atttccctga atcacaaaag ctacagagta tttagataga aatataccta ctcttaacat
361 gaaccatttt aaatatatgt attactgtgt
<210>17
<211>514
<212>DNA
<213>Homo sapiens
<400>17
1 tttttttttt tcctttaata cagaaacttc attgaaaaaa cctagccctg tacagctgcc
61 cttttaaaga ggtggccatt gcagggtctc tgcctcccca gcaggtggca gatgctcccg
121 gccactcgtt taaataactg tcccctggag gggnccgggg tttgaaggca gggccctgcc
181 accctcctgg gggacacccg tgcatgttta cgccttttct ggttgtgtca gtggccggag
241 catggcagct gggcatcaat aaacacctcc gaggaagctg gactcgtgtg tgctggcacg
301 gggagggcgc cgggcggggc cgtggtgggc atggcgtgat gtcacggtca ctccgggatg
361 tcgatcacca ggtcatcgtc tccatccaag gcatcgtccc cgctacctgc atcgctgaac
421 atctgccgag ggaccgtgct caggatcgtg ggggtctcac aaaccctcct agagagctct
481 gtgggtctct ctgcagtccc gtgctttgtc tgtg
<210>18
<211>502
<212>DNA
<213>Homo sapiens
<400>18
1 ttttttctcc ctcggcagga tatcccacag caagggcatc ttgaaaagca tgattttctc
61 ggtaatgttt gccaacactg ttcactttcc acatggtcac gactgaaaac acatttacca
121 atacctttca agcgatatga ctaccagaaa tagatcttct ttactaccct ctctgaaatg
181 agtacacaag aaataaattc agaaggtagg cttttgaaag aaaaagaaaa aaaattgctt
241 gcggcttcac agtgaaaaaa attggagtgt ttgtgccggt taagatttca atgttttctt
301 aatcaaaatt ctctgaagtc attctcaatt tacaaggtta gaactccttc aaagacaaga
361 catacaagac tctagcttta attatcattc taaatgtcca ctagccctgt atctttggtc
421 atgaaagcag caaaacattc acattttcat catactacat tcaattgcct tccaagttta
481 gaccattcta cattttaaaa aa
<210>19
<211>507
<212>DNA
<213>Homo sapiens
<400>19
1 tttttttttc agggcaggcg acctaatgta ggaaacttaa taaacccttg cattgatata
61 atgatctgtc aggttttcat ttgaacagtt ctccaacatt ctctgaactg ccaaataaac
121 tgaatctaag ccctattttt tcttatgcta aagtgtgaat gattctggac tatcttactg
181 ggaaaacatt tcctaacaat gtttttaaaa gtcataaggt caaaactgca gttataagaa
241 agaagggtga atgcagtggc tcatgcctgt agttccagtg ctttggaagg ctgaggcggg
301 aggactactt gaggccagaa gttcaagacc atcctaggca acacagcaag acctgtctct
361 acaaaaaaaa attttaaaat tagccaggtg tgacagtgca gaaaggtgag gtgagaggag
421 cacttcagtt caggagtttc aaggctgcag tgagctatga ctgtgccact gcattcctgc
481 ctgggtgaca aaatgagact ctatctc
<210>20
<211>557
<212>DNA
<213>Homo sapiens
<400>20
1 tttttttttt ttagcagccc ccacataatg actttatttg cttaatcccc aaattaaacg
61 ccgtttcaag cgagggtcct gtgttattac tctcatcatg tcgagaacat tttcctgccg
121 agaccaattt gaataaaaca aggggccttc cttgaactca atcaaggagc cataatgtgg
181 tgggtaatag aggttgctga tagatttgca ggcaaaagtg ttatacctaa tgatgggata
241 tggataatag tcctcctgga ggtcctgatg cgattccagt tggctgccag atgcttggct
301 cgaatgcgaa aattctccag gtgtctctga cgtgattcct ttagtatctt ctttagtcga
361 gtcttatggc aatataacag ccaagcaatg gcaatcacac ctgatgccca cttctgctgg
421 cgataaaaga ggaagaattt tcttgctttg tagcatttcc atgtggcttg gatcttcatg
481 gcggcctccg aatttccatc ttggcccttg tacctttgcc ctggaagatt tatcagcatt
541 tggacatgag ctgggtc
<210>21
<211>222
<212>DNA
<213>Homo sapiens
<400>21
1 tttttttttt tttttttttt tttttttttt tttttttttc ctttgacagt gaccatttat
61 tacacacatc agactggggg aagaccattg acatagctga gcaccaggta gggtgtcctg
121 ggtattcggg catgagagca gcaggggcct cctgacgcgg gcaggagaaa catggcaccc
181 accaggagga ggaagaggag gaagaggata aggaggaagg tc
<210>22
<211>534
<212>DNA
<213>Homo sapiens
<400>22
1 aaaaggattt aaaagtcttt atttcctctg aaaacttact ggtaaagtat acaatttatg
61 aataatcata tattcatgct ttttgaaata tatctgaata aaagacaaat tattcaatat
121 caatagcagt aaataactta tttgcaagaa aattattaca attcctactg taaagaatca
181 ccaaccacta tttagcaatt aaatttcaac acattttttt tggaactctt taggacttta
241 gacaaaggct accaaggctg acaatccagg taactatggc agtgacagaa gtcaatcatt
301 aatactgcca ttattttaga caaagggctg acaatctcag aaactagttc tagaaattat
361 catcaatgtg gccattttac tatggacaaa taaatatggg agatagggaa cttgttactc
421 cattttccta tagaaaaata tataaattga ccttttatat atacacaggt atgtatacac
481 atacagtata tgtttttctg agcaggcaca ccaactagga aaaaatacaa tagc
<210>23
<211>497
<212>DNA
<213>Homo sapiens
<400>23
1 ttttcacttg tttaaaacat ataatgcgat ggttttggta tttacagagt tgtgcaatat
61 cactacaatc taactttaga acattttcct taacccctaa ataaacctca tcctcatcag
121 cagtcacttc ccatccatcc caccccaact ccaaggccca aggatccact ttctgtctct
181 gtggatgtgc ctacggaatc tgtttctcaa cacacttctg ggtgattctg ctcctgcagg
241 tccacggtcg agcattcagg aaccagtgga ccagactgtt ccaagatcct ttcctagtct
301 aagctttcat agccggaaca atgaataccg acgaatangc aggtgctttg cttggtgctg
361 gggttgtaaa agaaatatgg tacaccctac tcttgagaac ctacagtcag tgggaaaaat
421 ggaatcataa atgactaact ttaatacaat gtgagaagcg ctctaacagc atgtgcattc
481 aaaagaggcc caggatg
<210>24
<211>304
<212>DNA
<213>Homo sapiens
<400>24
1 tttttgctat tcaaatgcac atttatttaa atgtttggaa ttaagagcaa cctagaaaca
61 gaaaacgtgt tcaaatattt attttaacag catcttttgg aacatgttta tttcccttaa
121 aaacgacaca gaggaaacat gtacactgta acaacacctt cccctctgtt tctccagaag
181 aaaaatgttt ctgcatgcct gataacagat ggtgcaacca acagtaaacc tggctctcta
241 caccagtgaa gaaccattct ccaaatgccc agtgtgcctc agaggaaata tacaatttaa
301 aagt
<210>25
<211>459
<212>DNA
<213>Homo sapiens
<400>25
1 tttggtttta taatgtatat attttaagta ggacaggttt tttttttttt aaacttttgg
61 atgttttata aatttgtcca atttagtcat ccaactgaca aagcaaagca accccgcgct
121 tgatccagtg ccgagtaggt tggtctgttt ttaacaacat tgcaatgaca aagttagtag
181 aatgtcccgt agtggaaaga gttcctttac gttcacttgt cttgtagagg ggacagacat
241 aggcatccga ctttataatc cgagattttt gagttggttt tatccatatg atgggcatca
301 ggtcaaacag aagtttggga tattgtcagc aagcaatcca ctttctcggt cccagcgtgc
361 gccatcgaga tacagtccgt ggatataaac accatcttct ggtgatgtgt cagatgtatc
421 agatgggata acctcaaatt catatcctag caaatcaat
<210>26
<211>459
<212>DNA
<213>Homo sapiens
<400>26
1 gcaggggatg gtgaggcact aacatcacag ggataattta tacaagatgg atgatattgt
61 agattcattt gcattgaagc caaatcctca atctcttctt catacatatt gctgctaaat
121 tgtattactc taattccaag ccagtactct tggctttttt gaaccataca ggaaggattt
181 agatttattc ctattactta tcaccctgtt agattcatca accatttgcc cctggccaag
241 gatgttttcc acttattgct gcagaatgct tctagtctct cccatgttta tgaaatccat
301 gatttgctct ttaggtactc taaatgctca caaaccatct gaataatact ttgggactga
361 taccaaactt tcagtctata atttacagca cacgtttccc tttaacttgc tcctgcttcc
421 cagaattcca cactgatcat aatcagtgat tactgatat
<210>27
<211>549
<212>DNA
<213>Homo sapiens
<400>27
1 tttatggcta atggtgacac actttgttaa tttaaaaaca cgcccttccc acatagtgcg
61 tgaggcatgt gcacattttc ctagaaggac atgaatagtg atgtggaggt acggtggagg
121 tcaggcatct acagggtcat tcgaggagga acagattcaa gctttcggac gatcagtgtt
181 ttgtaaatag cagcatcatc agatctaaga caacattgga cctggcaggg ccttttcttt
241 gggtggcatt aattactcca gattcagacc aggccatcct ctcccggccc aggatctgcc
301 tctccctcct ctgtccccac acctagacat gaaagccata acccttgggc ccctcagagg
361 gaaaacacaa ggggtgcgag gggtgggggg gcggcgatgg ggggcattgt tgagcagtcc
421 tctcacggct gggagcctct gctgcttcca gttaactgcc tttctggaaa gcgattctat
481 tacatgatgg aggcagcgga atgaaacatt gaattacacg gcacgagatc caagctagtt
541 gttctcatc
<210>28
<211>742
<212>DNA
<213>Homo sapiens
<400>28
1 ttttttaaca tgattgcctt ttatttatta tgcaggttgt tgatttacat agggagttgg
61 agatgctaac caagcatgga gttttcacat ggtctatttc tgctgagttc agggacttgg
121 agacagcctt taacttctgg caaaaagaca atttcacaaa ggtgtttaaa accatccttt
181 ggtttttgat cctgagtcag agacggacat gtgcttatga aagaaggtag agtttcaacc
241 cttaggtaac cttaaaagag caggaactat gttgtgtgta agtcatgtgc agtatacaaa
301 cttgatatta aatgacaaat tggaacaatc tttctctagg aatgcctctc tttcatagag
361 gcatcacagt gagtctctta aagccttgat ctaggtgtgt tacagatggg cttacagagt
421 atgaatgcac gataagaagg aaattggata gggagtgagg atatgaaatt taaaagaagg
481 aagaactgga acaaggcata ggcctctttc aatttttcgt ggtgtgcagc tgccaattct
541 tggacattca agaaacgctg gttgacatta tcttttttct tcacaatctg atcaacgttg
601 aaagtcccgc tggagagcaa atcttcagcc aatgtattca ggtccttgag tgcatnctct
661 cgagccaaca tctctctctt ncatagctga tgcttcttga gtaggtttct gctgaagcca
721 agtcctgggc tgatctttat gg
<210>29
<211>458
<212>DNA
<213>Homo sapiens
<400>29
1 ttttttttac catcactttt ttatttggca gtaatagtaa tacatccatg ttacagtgct
61 tattttggct aaagagccag tgcaattttt tctgctactg tagtgtttta cttttacaca
121 aattaatgag tacagtcctt acatttagga gacagaaaca tttactgaca tcagctaagt
181 aaagctaata catggttgtt cacaaactaa acaaaacaat aacataatat actcattagg
241 aacaaagtag ggatgttctt atttccacta atcacagatt atataaattc ttaaatttca
301 cttacatatt tctaaatcta agcaaaaaaa ttagaattca gcttaattcc tataatcatt
361 ttgaaattta catcaaataa tttctgaata tatattcaca ttaaaataaa agtaaatggg
421 ttatataaac ataatttaaa atttctgatt atgaaaag
<210>30
<211>542
<212>DNA
<213>Homo sapiens
<400>30
1 ttgagaaatt tttttattgt atttgttttc aagtgattct ggtaaccaaa gtattacagt
61 tacagggcaa gcaaacattt aagccaaaaa acaatcatat ggcaaaggcc tctgctttaa
121 tctactacat gagaggtcat tgttactcat ttccaaagct gttatagact tggaatgtgg
181 aattggagag gcttttcgaa tgtagttagt ggtgttcaca aaagaaccac tgcctttcaa
241 cttggaccct aagaatgaac ttatcacttc catttagaac ttatataagc aaaaatatct
301 gaaggcaaat atcaagccat gtttccacac tcccaaagta tctcatttgt catgagccat
361 gagtgtcact ttttaaactc cttccctgaa tgcccaatca acaggaaaag agcttgagag
421 caagggcaga agagtctaga ccaggtgttg ggaactatag ctcacagact ggccacttgt
481 ttttgtttgt ttgtttgttt gtttgttttt gagacagtct tactctgtcg cccaggctgg
541 ag
<210>31
<211>558
<212>DNA
<213>Homo sapiens
<400>31
1 gctttttttt tttttttttt ttctttatat aatttaatat ataccctatt aacaccagtg
61 tgcagatgta cagtactgac agtatactaa atagttagta ttaaaaatta ctttaaaaat
121 gttttcaaca ttaggtactg gtggaaccag agctgttatt ttgtacatca tatcatttat
181 gtgttgcaaa ggaaaagtaa aatttccagt agcattagaa aaaaatgtac tgatatttta
241 tgtaaatata caaaatctca tgacatctat tgaaaacctc acaattctat tgtgcactaa
301 taaaaacacc ttttcaaaca acttgcactt atactgagtt cataaaggct actataaaaa
361 tattgcaaaa gcaataaaaa cacaatatcg tacaaagtct tttgtctttt aaaagtcaaa
421 ttttaaaagg actgtattta tataaatgtg caanactttt tagtgaccct gactaacatt
481 tttgtttagg attatctgat tttagaaaac taagttaaat gtttttcctt attttctgtt
541 tcagaatgag aagaccta
<210>32
<211>528
<212>DNA
<213>Homo sapiens
<400>32
1 ttttttttac tttgagaagc catttatttt gcagccttca gtccaaaaaa agtcaacatt
61 ttcagaattt ttttatataa gttgtaggtc atttttataa caataaactt tctattatct
121 atttatctct cacatacatt tcatgtatcc tgagtattat gttacaacaa tctgctcttg
181 atagtaatgt tcctgataga ttaaaagatt gagaaatact tgaagaacga tcaaagatac
241 aatgagcatg gtatactttt gggttaaaat gtattctttg ataactgatg tcatatagat
301 ccctaagtaa atcaaatatt taatttccta catctgtcta ccttagttaa ctggcccatt
361 cctataagct taacaaaaac tcactttaca attatttaaa aaaaacattt aagagatttt
421 tagaagttaa atggaaagat acttttggac attacaatat ttttaatgag ttattatttt
481 aaaattagag atttcacata aaaaccagga ttctttgttt tctcttta
<210>33
<211>449
<212>DNA
<213>Homo sapiens
<400>33
1 aagatacagg gtctcaccct gtcgctcagt ctggaggtgt ggcatacagc cttgaactac
61 tggcctcaag ctagtctcct gctgcagcct cccaaagcac tgagattaca ggtgtcagcc
121 attgcacctg gcctttctct atccttcttt aagaatggta atttactgct ccagggaatt
181 ttgggaaaaa caggtgattt ttggtgagtt acggatcact gggaagagac cctaaacaca
241 tatacacgca tgcattcgag cacacacacg cacacatgtg catagacaaa taaaattagg
301 caaggagaca aaatattatt gaaaacaaag tacccacaca gacaataaca gatgcagcac
361 atctttgtgt ggctttccat gctaccaagt ccccaatggg gacttcacaa ctgctgatga
421 cacaagccca aggaagtgac atgccagat
<210>34
<211>536
<212>DNA
<213>Homo sapiens
<400>34
1 ttgcatgctg ctgcatggtt ttattcctca tgggtagatg aacacacact ggtatatggg
61 gaaatcctca cccgccctcc gctgctcttc tggaggtctg tcctcttgct ttcctcctct
121 gccccagcct ggtgctgccc caagccctgc cctggcaaag agagaactgt gcacagcggg
181 gaggctctcc aagccagagg cctcgcacgc agctgaggaa tgacgcaggg gcctgcaggc
241 agctcacgcg gagacaggtc tgtggggccc cgcgtcagaa cccacttcac atcccaaagc
301 cccacctgct ttgatcttca atgctgggct caatgtcaga tctggctgac cagaggggct
361 caatgaggct cactggctct tcatgcaccc tgctgtggcc tgagcacgtc cccccaaaac
421 ccacctgctt aagtcctcac ctgcaagggg atggtattag gtggggcatt tgggaggtca
481 tccggtcaca gggcaggggc acagcctcat catgggacca tcacccttac aaaaga
<210>35
<211>737
<212>DNA
<213>Homo sapiens
<400>35
1 aagaatcatc tgcttattta ttttacagag taatgattta aattgagtac atttgggcac
61 agtagatatt tgacacgaaa aatagttgta tcagaatgag catcagaaaa atagcaactc
121 atcttacaca taataactag aaaatattat tctgtctgat catttaagtg ttacaaatcc
181 tgagaatgtt atacattgaa caaattaggt actaccttat aattaaatct cgcttggaaa
241 gaatccttta aaaaatgctc tgaggtacaa atcacctatg tattaaacat gagtctctga
301 cattatacaa agagtttaaa caaatatatc aaacaattac aggccagtgg ctcttaaaac
361 agctatcagt caggttcaag gacacactgt tccacaattt cccgtaagtt ggggttttcc
421 attgcagctg ccactcgctc tttgtcattt atctgcttat tgatgtcttc ttctgttgag
481 tgggttcctt cagaaatgta gatttccaac ttatgtttaa atggtaaaca tcgctgaagt
541 tttactctta agcacagccc aataagagtc gccaaagagc aatgaggtac tggtggcgtg
601 aacctgataa taaccagata ttcttcttca tttattctcc tgaacttcca cacacctttt
661 ccgagaccac ttccagttct tcctaaagaa ttgggccttt ctggggcccg ggatagtcta
721 atcaaatcat aaacctc
<210>36
<211>582
<212>DNA
<213>Homo sapiens
<400>36
1 gatcaatacc acagtttatt tgtaaacatg ttatatgtgt caataatcaa attgacaaca
61 ctttatagat ttcattgtat aatattaaca tcctaacaga aaacgatcca ctgtactcag
121 ttacagtttg gtattttaaa atccttaaat acaaattgta tttgaaacac tgaacacaaa
181 aaagaaacat gaatggcaga gaaaactgaa aacaacaagt aaaagaaacc aatattccgc
241 tccccttgaa aaaaaacata aaatcatctg attacataat ttaaaaaaga aaccaaggga
301 attcagatga cattttttga tttaaagttg catttcttca aatccatttt tagaggtgaa
361 attgtatcaa tattaaaatt ctaatgtctt tttgngatat ttgttcttct ttcccctctg
421 gttcttaaaa gatggtgggc caggtttaaa ttactttaag ggaagggaaa aaagaaggga
481 ggaacttgga aaaagcccag gaaattctat tttcgcaaaa attgttggcg gaattttcga
541 gatctttgtg ggaaaaaaaa aggcgcttat taaaaacctt ct
<210>37
<211>451
<212>DNA
<213>Homo sapiens
<400>37
1 tccaatgaaa gtctatttat ttgctcgtaa tgagacagaa cgctacaatc tgttcaacac
61 tgggctggac actgcagtga ttaggggcag gtgtggggca gaaatagaaa tcacatgatc
121 gccactgatt cgcagtggag agggcgctga gctgggcccg gcaggcaggc agcctcagca
181 gagattcagg cagtcagcat ggtgcggccc tcccgccagc actgtcaggt cagcagaggt
241 tcaagcagtc agcatggcgc ggccgctagg accctctgcc atggctttgt gacactgcag
301 ctatgctggg cgggactccg ccccccaccc cacccctgca tcggagggca gggcaggcag
361 caagcagcat ttggaatgca gggtggagag gatgctagaa gtatgtgatg cacctgcggc
421 actgacagca tctctgccct cagaggctga g
<210>38
<211>532
<212>DNA
<213>Homo sapiens
<400>38
1 gattgggtaa aatatataca agataatgct atacttttaa aagagaaaaa gcactttaca
61 ttggttaggt ctttggatct gcatatgtcc ctgaggtaag tattccagcc atgggaaact
121 gaagcacaga gaggtagtca cccaccaaag ctcagggctg gcctccagag cttgaaccct
181 tgcctgagct ggggtcacca gcctgggtgg gccacggagc gatcatcatt tctccatctg
241 atcggaaagt cacccaccac tctgttctgg ggcccagccc gcctggctcc aggcaggctc
301 ggaggccgtg ctctcctgga cctggtgcat ctggctgcag ccagcactgg gcaggaaaat
361 accgctggag cagtgccagg tgcaggacgt ccccactacc tgcttcctcc tgggtctgga
421 gggccaccat gagcccacaa tcccggccag gcccaagccg gtggctgaag tgggcagctg
481 tgtctagaag taaggcaatg aggtaggcgg cttcctgggg ctctgggtct tc
<210>39
<211>334
<212>DNA
<213>Homo sapiens
<400>39
1 tttggtatag aaatgtttaa tacattaaat cttatgaggt aaaatacaaa aggtagtatt
61 cataaaagag ctattttgtt ttatttcact tgaagttctt aagagaaaaa cataagagga
121 gatatagaca gacctcatct gtaaactcat gttgctcttt acaaaggaag agacccagca
181 gcttttaagc cagttagtgg tagagacagg caagagtcac caaaaaaggt cattttagta
241 ttctgtttaa tggaacttag aaagagacta catcgtgggg ttgggggagg aggggggagg
301 gcantangga tntacctaat gtaaatgacg agtt
<210>40
<211>562
<212>DNA
<213>Homo sapiens
<400>40
1 tgcttaacaa gcagttttat tcctaacaga caaaaataag aagcaataac atatccataa
61 gcagatgaat tcgtatacag tgatttccat agcatgtcat attctaagat aaaaagaatg
121 aactactcat atatcatgat tgatgaatgt caaaatatgc atccctgcta tgtgaaataa
181 aaataataat aaagaaaaaa gaagaacatc taaataaata gggctcagga gagtactcag
241 gtatgaaaag aggaaacatg ttatatcagt catataaaat tccagaaaat tgacattaat
301 ctactgttaa gcaaatattg taattcagtg attgtctgga ggatggagag gccagagaga
361 aagatagtat ttatttttta agagacaggg tcttactatg ggacccaggc tagtctcaaa
421 cttcttacct caagcaatac tcccaccctg gactcccaaa gtgttgggat aacaggcatg
481 agtcaccatg cccagtctta ttacagcttt aaaaatttaa tttgaaagaa ctatctggat
541 ttaacattat ttttgacaaa ta
<210>41
<211>542
<212>DNA
<213>Homo sapiens
<400>41
1 gcggccgcgg gacgcgctgg ggccgggcag ccgggaagcg gggcgctgac ggtgagtggg
61 cctggccccg ggccggtggg tccccgcggg gcggccgggc gaggagagag gcagcttccc
121 ttcccggagc ccgcctgggg cgtggaggct cagcgtccct atcctccatc ccgccccggg
181 acggcggaac cgggcctcag ctggggacct caccaggggg cggggcctcc cgcggtcccc
241 ggcgctgctc ccaggactgg aggggcgtcc gcgcccggga gaggggttcc ccccgacagg
301 gccccgggcc gccacgaagt tagggaagcg ctctctccag ggtggaacct gggcccgcgg
361 ctcccgggag aggagctgcc cgcggaaggg gtcggngtgg gctccaggct gggagaagga
421 tctgactntg ctgacctcgc aggctggtag gggaagctgc cccccagagc agcatggatg
481 ccccgcgaag ggacantgga gttgctcagc aacagcctgg ctgcctacgc gcacatccgc
541 gg
<210>42
<211>561
<212>DNA
<213>Homo sapiens
<400>42
1 tttttgagcc aagaagtgca gtaggctgcg gggattttat ttctgccccc agaccaaggc
61 aacggagccc tcacctgcct gccaccccca cactgggccc aagcaagctc aggtgagcgc
121 acatacatcc accgtggcgg gctcctctcg ggtgaagcca tcaccgaagc cctcatcgtc
181 caccaggtcg ggctcacggc agcacatggg acagaggctg ttgcgcacag cagaggctcg
241 gagccagacg acaaggttcc aacgctcacc agtgcccaag ggccgggctc catgcagctg
301 gccgccacgg tggaggacac cctggcccac cacgtgctcc acctccaggg gctccgtcag
361 ggctgtgggt gcctggaaga ggcccccaaa atacagggcg ccccctgtga agaccttgcc
421 caaggccaca ttgagggtga gctcggcatt atcatagtgg cagcccagct ccaggtcctg
481 gcccggtgcg tatttgacca caaaggcccg gtggctgtcg agccggcccc cgccacagtc
541 agggtacagc agggccatca g
<210>43
<211>484
<212>DNA
<213>Homo sapiens
<400>43
1 tatgatccaa ttacaaaaaa gttatctgat tttatttctt atatatttgg cacaaacagc
61 agagactgaa agctactaca catgttgaac tgctgacagg aaaagaaccc agtagataaa
121 tctaggcttt ccactccata tattagctgg ggctgccata acaaaacatc atagactggg
181 tggcttaaac aacaatttac ttctcacagt tctgaagtct ggaagtctga gaacaggtcg
241 ccagcatggt taggttctcg tagggttctc ttcctgttct gtagacagct ggcttcttcc
301 tgcatcctca catggtgcag agagcaagca ggctttctga tgtctttttg agacgagtct
361 cgctctgtcg cccaggggtg agtacagntg gcggatcctg gctcactgca acctccgcct
421 cctggttcaa gcgattcttc tccttcagcc ctccgagtag ctgggactac aggcacatgc
481 cact
<210>44
<211>544
<212>DNA
<213>Homo sapiens
<400>44
1 tttaaaaaag attacaaaac tgaatttatt gagattcaca caagatgcac ttataaaatt
61 agtactgaat gccattaaaa cagaagaaat gaacataatc ccgaactccc aacagcatct
121 gcaaaggaat ggaaatcttc tgaaaatgac agcgcagtaa cagaataatt caagcggaac
181 tgaagatcta tccaaaccat gttcctgctc tgaaatcagg gttgtgtttg gcaaagcttt
241 ccccaaacta ttccattcac ggtggcattg ttccttttgt tggtaaaggg agacggaaaa
301 aataactcta aaatggggct ccattaaaac acacacaaag caattaaaag ctttttcatt
361 tttaacttaa aatacttcct ggtttcacaa aggcatgtcc atacaatttc ctcaacatta
421 atcttcagat ttctttagtt aatgcaaaac attaaaattc acttactttt ttgtatttca
481 aatgaacaag tcagaacatt aaagcttgct ttatatttca tccaagagac agacaaatca
541 ccta
<210>45
<211>587
<212>DNA
<213>Homo sapiens
<400>45
1 tttttttcac aggtttgtct tttaatcagc atttttgaaa attcattaaa aggtctgata
61 cgaaaagcac caggctaaca aatcagtcct gaattcaatt ttacaaaaac accctctcct
121 cttccatctc atccctaata ttcctacgtt atctcgaggt gcagagaaat ataaaaggga
181 tccttgacat aaaaacgtat taaaaagcca ccatgagtag aatgtacggt aacagcgtct
241 gactcggccg gcaggacagg aaacctacag aaccaccatg ccagggcttt gtgtgagcag
301 gcgggcactc cacatgggca cgtgtgtgag caggcaggca ctccacatgg gcacgtgtga
361 gcaggcgggc actccacatg ggcacgtgtg tgagcaggcg ggcactccac atgggcacgt
421 gtgagcaagc gggcactcca cattggcacg tgggagcagg cgggcactcc acatcggcat
481 gtgtgtgagc aggcgggcac tccacaatgg cacgtgtgag caggcgggca ctccatatgg
541 gcacgtgagc aggcgggcac tccacgtcgg cacgtgtgag caggcgg
<210>46
<211>535
<212>DNA
<213>Homo sapiens
<400>46
1 tgtagtggca agtgatgtat ttggcttaaa gaagcttgtg ttactgaagt taacgcagaa
61 gtctggcctc cgtttgtggg attattaaat atatatccta cgggatcaga gtcatctatt
121 agaaacagtg atagccctgg gtataaataa acatttcttt agagtttggc gtcagttttt
181 caatgtccat cctcaaatca gacagtctgt ttgaggggtc agttttcagg gtgcatctgc
241 accgagaggc ttgaggcagc aatgacccct gggctgcagt tcggttgcag gcgctggtgg
301 cgggcagtcc aggttggagg gtggagatgg gccaaggtca cagatgctgc agggcttcct
361 gtccttgctc tgtgtaaccg tcatcgcggg ggggggttgg gcacttgtcc aaagggaccc
421 ggggatgcgc acgttccttc tgtgtgtggg ccaggcgctt cttatctcgc aggctgccca
481 tctcctgctc cttggtctgc aggtctgcct ccagctccac cttctgttcg atcag
<210>47
<211>469
<212>DNA
<213>Homo sapiens
<400>47
1 ggcatgtcat cattttaatg atgtgatctt tggtgtttcc ctcattagct gtagactatc
61 ccctctcctc ccaccacaat gtttctatga tgagttacaa acagaaagga aatcacattt
121 tcatactaaa aacaaaatga tcagagcctt gatttctcca ctagaaacta cacgtacagt
181 taagagtcca catgcaacac cttaaatcac agactgagac ctcacattct gacctggagt
241 ctcctcccct tccccagcct tgggctagct ttggcctagg ctcaggtaat actgacaccc
301 acaggcgctg ctctgagggc cttgcgggga gaagactggt ggacaagccc tgggggctgg
361 ccagcctaca ccccccactc ctgagtgagg acctgcccac tgtcccttga ccccatcctg
421 tatcaggcaa gcaggcgccc ccttacccca cagtantccc ccattagta
<210>48
<211>427
<212>DNA
<213>Homo sapiens
<400>48
1 tttttttttt tttttttttt tttttttttt aaacagggtt tttaattgaa aataaagcta
61 aagcatgctt ttcaacaggg caaatttcca taattttgat ttactggttc catcatcaca
121 acacagtaca ccagcatggc ccttacccag ggctagaccc tagcatttgc atgttgaact
181 actttgattt taagggcaaa tataaatagt cacctctgat tgtcacttgc agtaaaaact
241 ctcctgcttt atacacagga tggtctgaaa acacaagttt tagagagatg caaaaaaagt
301 ttaaggtcat aaaatccaag ggcctgagtt ctggtcccaa aaagccctga aaaaactggt
361 atttttcatt tggggtattg caactctgac ccctcatttt ctttttcttg gggggattta
421 ctgattt
<210>49
<211>508
<212>DNA
<213>Homo sapiens
<400>49
1 tttttaatag tgtcttgctg ttactcagac tgaagtttcg ccatcatagc tcactatagc
61 ctcaaactcc tgggctcaag ctatcctcct tcttcaccct cccccaccat gctcggctaa
121 tttttaaaat ttttagtaga aatgaggtct tgctgttttc caggctcgtc tcaaactccc
181 aatctcatgc aatcctcctg cctcagcctc ccaaagtgct gggattacag gtgtgagcca
241 ctgtacccag ctgagagctt aagtttcttg cataaaactc agctgtatgc tgaagtgttt
301 agaagtgaaa tgtaatgatg tctgcaactt actttgaaat acattaataa aatggataaa
361 tgaatggata gagggatgga taggcagatt gacatgtgta aaacaaatag agaaaaatgt
421 tatttgtaga atctaggtga tgctaaaggc cacacagcca gtggggactg agccaagcta
481 ggaatccagg tccaccaatt caggagag
<210>50
<211>507
<212>DNA
<213>Homo sapiens
<400>50
1 ttttttagaa aagtcctttt tatttcttta aatcagaggc cccctctagc gtttgaaagc
61 agtcacaggc aacacacaat gcaattgaaa gtaaacagtc acagcatttt accgcagacc
121 ttccaaaaca taaggcttgc ttctgttcca ttcaagtggc acaaaaatca atgagagaaa
181 aatagctaac cataattaat attgcaaatt cctgctatga ttcagattca ttatcctctc
241 attcagtaat ttctttatta tttactgctg ggttattttt tactagatta atactctgtt
301 aataaggaaa aaatatataa taaatatagg gactttaatt tccctaataa gaaatatacc
361 tcatttgaaa tgaccgaaaa ttaatgttac catatatcct actctgcttt gatggaagtt
421 attggtaact tcctaaagta tatacttaat tttaacttta ttatatttgg aaatcagaac
481 tttttaaagt acatatattt tgaaatt
<210>51
<211>541
<212>DNA
<213>Homo sapiens
<400>51
1 tttttttttt taaaagaaat ggatgctttt attcaacctg agttaaaacc acaatgaatc
61 atatcagagt tcatgggaat cataataaat gactagtaaa ccactcacaa aatcaaagct
121 atcaacatac taggatcaca cacattaatt agtgctatag ttcaaaacat accctttaac
181 ttatttagca cgcaaaataa tcataaaagt aatgtctata agctctaacc aaaaatttta
241 aaaattaaaa atagcacaat tctacaattc tgattttacc aagaaaataa accttttttg
301 gcacatatta tcctatgaaa atggaaagct gagtcaggct gctctgcttt tcacagcaca
361 aataagcatt catgctatca gacttgggaa attaactcgg tgacaaaaat tcactggaaa
421 atagaatcct tggaaaaatg gggtcaggtg ccatccactg agaggcaatg ataatgtgtg
481 tccttcgtta ttagcacaaa gttaggcagc acactataat tntagctaca tgcaactcta
541 t
<210>52
<211>369
<212>DNA
<213>Homo sapiens
<400>52
1 tttttttttt tttttttttt tttttttttt tttttggcta aaatttacca catttattcc
61 aaattgggat tttataacaa ggagaagtct ttaaaatact aagggggcaa aaaaatacat
121 aatgggagac tgacatattt tcaaacttaa gtcacattgg catgaatcaa aacaaagtta
181 ccccaactaa aatacatata agaaagagcc actgaaggaa aagcatcaca agacacagca
241 acacacttca gttcatttgg aaaatctaaa attaagggtc aaagcagctc acttaattga
301 atctgatttt caaaatacac atttcaggga caagcatttc cacttacctg cttcattatg
361 ctcttaaaa
<210>53
<211>298
<212>DNA
<213>Homo sapiens
<400>53
1 tttttttttt tttttttttt gggtttgacc catttttata gaaagccagc taccaaaagt
61 aatgagtccc cagggccaac cacattggag gcctgggtga gggaccaccc agcagggagc
121 aagggaggga tgcagacaaa ggtagcagag accctggcaa cctccttcca gtactcagca
181 gtcacaggtt aactgtggga gaacccagca acacactggg tccccagcac agtcttgggt
241 gggcccttca tggaaggggc agggaggctc tggatgcttc tgcagtcatg ctgaccca
<210>54
<211>520
<212>DNA
<213>Homo sapiens
<400>54
1 ttgtttttta gtataaagag tagtatttta ttgaataaga tttgctcaca gaaaaataag
61 cttacatcta caatgaatgc cagaccctac agcagaaagc cattttctca cttttccaca
121 cacaatggtt cctactaagt gaaaaaaaaa gccataaaat ttcattcaca aatgtactac
181 tctgtctcaa aacatcgcac ataatcatgc actgtactaa agccattaga tcagttcttc
241 agtcaggtta aagaagtatc cctctaataa ctgactttta taatgctatc aatatccact
301 cccaatcagt ctgccattgt taatggtgta cagcattact gaatacaatg gaattgatga
361 cgcccatatc cacggacaaa ccgtgactta tgatggtttg atttacgatt tttcaacgtt
421 atgatgggtt tactggaata ttagatgcgt ttctgagtta cactgggttt atgagtatgc
481 gaccctatac tccaganaca gctgtagaag gaaaaacagg
<210>55
<211>166
<212>DNA
<213>Homo sapiens
<400>55
1 tttttttttt ttttttcagc agcacaaatc ccttttatta accatcttcc ctttctcgag
61 tatacacaag tagaaaagaa acagggaagt accccgatag ggagggtggg gagaggatgg
121 gagggggctc ctgaggttag gacaagaggc cagattccag ctcctt
<210>56
<211>462
<212>DNA
<213>Homo sapiens
<400>56
1 tttggttttt gtgatgctgg aagaatgacc agaatgagtc ataggaagag tatacattcc
61 tgtagtattt tccaggacag aggtgtgacc tcctagagta ctgggaccaa aattcccaag
121 tgtctgcaac cttgctttaa cagtatggga gatcacctct atcacctgga attcccctgg
181 aactctggaa tatacaagag aagtatgaga cttgggtctt cccttggctg tgtttaattc
241 actcttctat ggaataccaa tgattctcac taagactggc cttttcataa gcacaatgtg
301 cattttatgg agaagatttt acactttgct ctatttagaa agaataaata tgagcagtgg
361 tttaggtttt atgccctgga cttaatatgt ttctgattcc tgttttgaga ttaaattctc
421 atgtaaatag aaaaatactt attatttctc ataaggccaa gt
<210>57
<211>424
<212>DNA
<213>Homo sapiens
<400>57
1 tatgaacaga gtggtttaat gaaactactt tttttttgtt tttgtttttg ctttcctttt
61 cccataaatg ccatcgtttt ggtaaatgtt attactacag cctttttttt tttagttctt
121 tttttgagaa ctaccattta ttgagaatgt cttattccag gttcctacat gggttttcat
181 aactactgtt ttcaacaact ttgagacaga cataataaat aatgtagtaa acagcatgga
241 tataccctca gatctctaaa acccatggtt ttggcacaat gccattccaa cctctgccat
301 cttcatcctc ctgctttaac caggaaaaca tatttactgg gcagtagaca tgtcaaaggt
361 gaaggtattc atatccaaat aatactgtgc tgttgttttc aaagcaatcc atgtttcacg
421 agcc
<210>58
<211>515
<212>DNA
<213>Homo sapiens
<400>58
1 tttttttttt tttttagaag ttatgattaa attttattgg ggcataaaac ttgtgataca
61 ttgtaaataa aacagtagca aaacataatg ccagttgtga aacgtctgtg tatacgtgca
121 catgctattc tggttctttg gtttggtttg gttttttttt tgaggcagag tctcaaaaaa
181 aaaaaaaaaa cgaaacaaaa ctccagtgtc ttaatgtcct ggcgtgccat ctcctggtca
241 actggtaaag cagcaatcct ttcctataag ccagtggccc tttggagaag ggcacactga
301 acacaataag catgactttt agttttgtat ttttctccac ctctccctga tgctaattca
361 tacaatatta aatttcaaaa ttcagcattc ttgttcttcc agggagcatg aataggaatg
421 aaccacacct aatgaagtag gccctatcat tttattgatt ccttcccaaa gatcataatg
481 aaaggcctgt aagaaaagca taaaatcaac agatc
<210>59
<211>604
<212>DNA
<213>Homo sapiens
<400>59
1 tcagataatc aaaataattt ttatttggtt gtagaatgtt gtagtacata aagcaaaatg
61 tgtctgcagg atactccaaa tttaccaact tcaaacaaaa taatgcacag tgaagaatta
121 gaaaattatc aaccttactc aatacatgga agtattcctt aatcttcatc attttcatta
181 aacaaaataa tattttggat gagttcaaca tttctatcct atgctttatg tttagtgcct
241 tccctgaatt ggaaaaaatg aggtgtttta tttaaattta acactggtct aagggagata
301 tagaattaaa ctaaacaaga atcggttatc acaacctcac aacatatatc aaagtattga
361 gattgttggt ttactgactg tcacttttca ctggatatta aatatcctga gggctacaac
421 tgtatttttt aacctgtccc agtacctcaa caaaattttc actgaatgta tgaataaatt
481 atgttaacaa aaagttaaca ataaaagtat aatgaagatc cctttaatta attntaaaat
541 ctgaaaggaa aactggagtc acaaaacata tgttcaaatc ttaattcatc acaaattttg
601 tgat
<210>60
<211>792
<212>DNA
<213>Homo sapiens
<400>60
1 tttttttttt tgtttgtaaa aatcggcatg ctcctttatt tgttcagaag agcagccagc
61 atcaccctcg ccactcaaac ctggcacata cgcttcggag acaatggcct cgggaccctc
121 atgctgctgg gcccaggaga gacagttctg aggcagaaac tcggcgtcca aggggggccg
181 cgggtcaggc actgtggtga agggaacgcc ggggagtccg gccccacctt gcagctgggg
241 acacgggggc gcaaacagag ggggcaggct agtgtcccgc tgccccagga acagacctca
301 gggccccagg agggtctgca ggcagctagg agcctgccaa gtgctggtgg aagtagaggc
361 cgaaaaggct ggcgagcagc tggcaggcag ccgtccacca gatgaggtag gccaggacgc
421 cacggaggaa gaggggagta agcaggccac ccagggcccc ggcaatccgc gctgcagtct
481 gctggacttc gtcctcccca gagccgatgg gggcagcgct ggctgaggat gggtcggggg
541 atagtagagg agtgggccca ggccccagga atagcctccc agcgtcttga gagagtgtgc
601 gtttgaggtg agatgagcgc gtcagtactg cagctcacca ggtcacatag agagactcga
661 ccacctctgc tgattcacgg gctcatgcgc cagcctctcg tccgaatctt gcctgaggca
721 aatcccctat gaagcgataa atcgaatcag ncaagtggnt ccgtggaatg tatcgtacat
781 tccccacata ca
<210>61
<211>558
<212>DNA
<213>Homo sapiens
<400>61
1 tttttttttg gttacaggtt ttactgtctt acagtgtgaa taccccatga aatcgtagaa
61 ctctttgcaa cttatgtcac acctgtatct tcaagggcaa ggattatttg ttgctatagt
121 ttttatgtca ctaaagtacc ttttagtgtc atatacatag tagataacac tggataataa
181 taataaaaaa gactaagaat gctaaagtag tctaggaatt cctaaatctt aataaaaact
241 gagaaataaa gttgtgatac tatataacac ttaaggcacg agtaattttt ttttcaccgt
301 ttcccctcca tggctatgaa ggaactggga gaaggatgct cctttcatta attaaaattc
361 ttcactttga aaatttaagt ttattaggga aggaaggaag aaaaatatga agatgtaagt
421 atacatacct tggagtaagt atttccttat attggagttt ctctaattta gctggggcta
481 ctaatgacat gggaatcaca atgttatcta tgtcataaga gctctcactt ctcaatctcc
541 gtcgtgcagt attctgca
<210>62
<211>478
<212>DNA
<213>Homo sapiens
<400>62
1 ttatgcaatg ccttgcgttt atttcggaga cgtgattggt gcctagactt ctaaagtaac
61 agaatttgat atggagtgtt gaaagctgaa ctttatcaat tatcacagca aaaatctaag
121 taagcgcccc gtgagaaccc taaaattaat aaaagctatc ttgtatgggg cacttacagt
181 tccacaggca cttgtaggcg ctttattcac aataatcgca gaagagcagt ataaagagca
241 tgcactctgt taccaaacca catccctgct ctacaggtgc tcccttagcc catctgtgcc
301 tcaattccct aagccgtaaa acggaggcct tgttctgacg ctgtgataat ttagtgcggt
361 aataggaatg atgcactgaa cacaaccctg gagcatagta actgattaat attagttaga
421 atattcatta tgatgataat gattggcaga gccctcgctc ttcacatgta ttcccaaa
<210>63
<211>211
<212>DNA
<213>Homo sapiens
<400>63
1 tttttttttt tttttttttt tttagatagg aattgggttg caaaaagccc cttttgttta
61 ttttattttg gaaaaaaatt ttattgggaa aattcacata tatatataaa aaattcaatc
121 aatacaaaag gatagacaat gaacaaatga attccccttc cactccagat ccccacccca
181 gatccagacc tcctgagccc actttcccca t
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| 表达序列 | 2020-05-11 | 388 |
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| 时间序列数据的多表示存储 | 2020-05-12 | 327 |
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