Method to beneficially influence & alter the development & life cycle of agronomic and horticultural crops with certain imidazolinyl nicotinic acids and derivatives thereof
专利汇可以提供Method to beneficially influence & alter the development & life cycle of agronomic and horticultural crops with certain imidazolinyl nicotinic acids and derivatives thereof专利检索，专利查询，专利分析的服务。并且There is provided a method for increasing axillary branching, tillering, flowering, and yield of agronomic and horticultural crops, by applying to said crop plants or to soil containing the seeds or other propagating organs of said plants, an effective amount of a 2-(2-imidazolin-2-yl)pyridine or 2-(2-imidazolin-2-yl)quinoline derivative.，下面是Method to beneficially influence & alter the development & life cycle of agronomic and horticultural crops with certain imidazolinyl nicotinic acids and derivatives thereof专利的具体信息内容。
This invention relates to a method for increasing axillary branching, tillering, flowering and yield of agronomic and horticultural crops by applying to the foliage of said crops or to soil containing seeds or other propagating organs thereof, an axillary branching, tillering, flowering or yield increasing amount of a compound having the structure:
- R1 is C1-C4alkyl;
- R2 is Cl-C4 alkyl or C3-C6 cycloalkyl; and when R1 and R2 are taken together they may represent C3-C6 cycloalkyl optionally substituted with methyl;
- A is COOR3, CONHR6, CHO, CH20H, COCH3, COC6H5, CN, CH3, CH=NOH, CH2COOH, CONHOH, CH2CH2COOH, CHR8OH,
- R3 is hydrogen, diloweralkylimino
- C1-C12 alkyl optionally substituted with one of the following groups: C1-C3 alkoxy, halogen, hydroxyl, C3-C6 cycloalkyl, benzyloxy, furyl, phenyl, halophenyl, loweralkylphenyl, loweralkoxyphenyl, nitrophenyl, carboxyl, loweralkoxycarbonyl, cyano or triloweralkylammonium;
- C3-C12 alkenyl optionally substituted with one of the following groups: C1-C3 alkoxy, phenyl, halogen or loweralkoxycarbonyl or with two C1-C3 alkoxy groups or two halogen groups;
- C3-C6 cycloalkyl optionally substituted with one or two Cl-C3 alkyl groups;
- C3-C10 alkynyl optionally substituted with one or two Cl-C3 alkyl groups; or,
- A cation selected from the group consisting of alkali metals, alkaline earth metals, manganese, copper, iron, zinc, cobalt, lead, silver, nickel, ammonium and organic ammonium;
- R6 is hydrogen, hydroxyl, C3-alkenyl, C3-alkynyl or C1-C4 alkyl optionally substituted with one hydroxyl or one chlorine group;
- B is H, COR4 or SO2R5, provided that when B is COR4 or SO2R5; A is COOR3 in which R3 is other than H, or a salt-forming cation, CH3 or CN; W is 0; and Y and Z are not alkylamino, hydroxyl, or hydroxyloweralkyl;
- R4 is C1-C11 alkyl, chloromethyl or phenyl optionally substituted with one.chloro, one nitro or one methoxy group;
- R5 is C1-C4 alkyl or phenyl optionally substituted with one methyl group;
- W is 0 or S;
- Ra is C1-C4-alkyl or phenyl;
- X is hydrogen, halogen, hydroxyl or methyl, with the proviso that when Y and Z are taken together to form a ring and YZ is represented by the structure: -(CH2)n-, where n is 3 or 4, X is hydrogen;
- Y and Z each represent members selected from the group consisting of hydrogen, halogen, C1-C6 alkyl, hydroxy-loweralkyl, C1-C6 alkoxy, C1-C4 alkylthio, phenoxy, C1-C4-haloalkyl, nitro, cyano, C1-C4 alkylamino, diloweralkylamino or Cl-C4 alkylsulfonyl group, or phenyl optionally substituted with one Cl-C4 alkyl, C1-C4 alkoxy or halogen; and, when taken together, Y and Z may form a ring in which YZ are represented by the structure: -(CH2)n-, where n is an integer selected from 3 and 4, provided that X is hydrogen; or
and when W is O and A is CN, CH3 or COOR3, provided that R3 cannot be unsaturated alkyl and Y and Z cannot be alkylamino, dialkylamino or alkylthio, and the N-oxides thereof, and when R1 and R2 are not the same, the optical isomers thereof, and, except when R3 is a salt-forming cation, the acid addition salts thereof.
This invention also relates to a method for effectively inducing and/or accelerating the maturation of cereal crops by applying to the foliage of cereal plants and/or to soil containing seeds of said plants, a maturation accelerating amount of a 2-(2-imidazolin-2-yl)pyridine or 2-(2-imidazolin-2-yl)quinoline compound of formula (I).
Application of the formula (I) 2-(2-imidazolin-2-yl)pyridines or quinolines to the foliage of a variety legumes or herbaceous ornamentals, or to soil in which said plant species are grown, at rates between about .0001 kg/ha and 0.01 kg/ha, also has the advantage that such treatment frequently induces a mild dwarfing effect on the treated plants. This dwarfing effect is particularly advantageous in the treatment of ornamental plants wherein the aesthetic value of the plants can be measurably improved by reducing their height and increasing their canopy.
A preferred group of 2-(2-imidazolin-2-yl)-pyridine compounds for use in the methods of the present invention has the formula shown as formula (I) above, wherein R1 is methyl; R2 is methyl, ethyl, isopropyl or cyclopropyl; W is oxygen; B is hydrogen, CO-alkyl C1-C6 or CO-phenyl optionally substituted with chloro, nitro or methoxy; A is COOR3, CH20H or CHO where R3 is as described in formula (I) above, X is hydrogen, Y and Z are each selected from the group consisting of hydrogen, C1-C6 alkyl, Cl-C6 alkoxy, halo, phenyl, nitro, cyano, trifluoromethyl or methylsulfonyl; and when Y and Z are taken together, YZ is -(CH2)4'
A more preferred group of these 2-(2-imidazolin-2-yl)pyridines may be illustrated by the formula (Ia):
The most preferred formula (Ia), 2-(2-imidazolin-2-yl)pyridine compounds are those wherein B, X, Y and Z are each hydrogen; A is COOR3 and R3 is as described in formula (I) above.
A preferred group of 2-(2-imidazolin-2-yl)-quinoline compounds useful in the methods of the present invention is illustrated by formula (II) below:
A more preferred group of formula (II) 2-(2-imidazo- lin- 2-yl)quinoline compounds are those wherein X, L and R7 are each hydrogen; R1 is methyl; R2 is methyl, ethyl, isopropyl or cyclopropyl; B is hydrogen or COCH3; A is COOR3, CH2OH or CHO and R3 is as defined in formula (I); W is oxygen and M and Q each represent a member selected from hydrogen, halogen, methyl, methoxy, nitro, CF3, CN, N(CH3)2, NH2, SCH3 or SO2CH3, provided that only one of M or Q may be a substituent other than hydrogen, halogen, methyl or methoxy.
A still more preferred group of formula (II) 2-(2-imidazolin-2-yl)quinolines are those in which R1 is methyl; R2 is isopropyl; W is oxygen; B, X, L, M, Q and R7 are hydrogen; A is COOR3 where R3 is C1-C8 alkyl, hydrogen, C3-C8 alkenyl, C3-C8 alkynyl, C3-C6 cycloalkyl or a cation selected from alkali metals, alkaline earth metals, manganese, copper, iron, zinc, cobalt, lead, silver, nickel, ammonium and aliphatic ammonium.
In formulas I, Ia and II above, alkali metals include: sodium, potassium and lithium, but sodium is generally preferred. Further, the term "organic ammonium," is defined as a group consisting of a positively charged nitrogen atom joined to from one to four aliphatic groups, each containing from one to 20 carbon atoms. Among the organic ammonium groups which are illustrative for the preparation of the aliphatic ammonium salts of the formula (I) imidazolinyl nicotinic acids and esters herein are: monoalkylammonium, dialkylammonium, trialkylammonium, tetraalkylammonium, mono- alkenylammonium, dialkenylammonium, trialkenylammonium, monoalkynylammonium, dialkynylammonium, trialkynyl- ammonium monoalkanolammonium, dialkanolammonium, tri- alkanolammonium, C5-C6-cycloalkylammonium, piperidinium, morpholinium, pyrrolidinium, benzylammonium and equivalents thereof. Exemplary of halogen hereinabove are chlorine, fluorine, bromine, and iodine, but chlorine and bromine are preferred.
In accordance with the process of the present invention, formula (I), 2-(2-imidazolin-2-yl)pyridine esters, wherein A is CoOR3 and R3 represents a substituent other than hydrogen or a salt-forming cation, and R1, R2' X, Y and Z are as described above, can be prepared by reacting an imidazopyrrolopyridinedione, represented by formula (III), hereinbelow, with an appropriate alcohol and corresponding alkali metal alkoxide at a temperature ranging between about 20°C and about 50°C.
In these reactions, the alcohol can function both as reactant and solvent. As such, a secondary solvent is not required. However, when an expensive alcohol is employed in the reaction, a less expensive secondary solvent, such as dioxane, tetrahydrofuran or other non-protic solvent, may be added to the reaction mixture. The amount of non-protic solvent added to the reaction mixture may be widely varied.
The overall reaction can be graphically illustrated as follows:
Advantageously, the formula (Ib) 2-(2-imidazolin-2-yl)pyridine esters can also be prepared from a formula (IV) dioxopyrrolopyridine acetamide, wherein R1, R2, X, Y and Z are as described above, by cyclization thereof with a strong base, such as 1,5-diazabicyclo [5.4.0] undec-5-ene (DBU), in the presence of an inert organic solvent such as xylene or toluene to give the crude imidazopyrrolopyridine of formula (III). The reaction mixture is heated to a temperature between 100°C and 150°C, and water is removed from the reaction mixture during the reaction using any convenient means, e.g., a Dean-Stark water separator. At least one equivalent of alcohol, represented by the formula (V) R3OH. wherein R3 represents a member other than hydrogen or a salt-forming cation, and R1, R2' X, Y and Z are as hereinabove described, is then added to the reaction mixture and the thus prepared mixture heated to reflux at a temperature between 100°C and 150°C to yield the formula (Ib)2-(2-imidazolin-2-yl)pyridine ester. The overall reaction can be graphically illustrated as follows:
In still another embodiment relating to the preparation of the formula (Ib)2-(2-imidazolin-2-yl)pyridine esters, the cyclization of a carbamoyl nicotinic acid ester, represented by formula (VI), with phosphorus pentachloride at an elevated temperature, generally between about 600C and 100°C occurs. The reaction is preferably conducted in the presence of an inert organic solvent, such as toluene or benzene. Good yields of the hydrochloride salt of the desired formula (Ib)ester are attained. The hydrochloride salt is then readily converted to the formula (Ib)ester by dissolution of the acid addition salt in water and neutralization of the thus-prepared solution with base, such as sodium or potassium carbonate. The overall reactions can be illustrated as follows:
In still another embodiment for the preparation of the formula (Ib)2-(2-imidazolin-2-yl)pyridines esters of the present invention, the cyclization of a carbamoyl nicotinic acid ester represented by formula (VI) using a mixture of phosphorus pentachloride and phosphorus oxychloride is accomplished. The reaction mixture is stirred at room temperature from about four to eight hours and then the POC13 removed in vacuo. The remaining residue is dispersed in an organic solvent such as toluene. The solvent is removed and the residue dispersed in water and heated to between 80°C and 100°C. After cooling, the pH of the aqueous mixture is adjusted to 5-6 with sodium bicarbonate, and the product extracted into methylene chloride to give the desired formula (Ib) 2-(2-imidazolin-2-yl)pyridine ester. The reaction can be graphically illustrated as follows:
The formula (Ib)2-(2-imidazolin-2-yl)pyridine ester in which A is COOR3 and R3 is alkyl C1-C12, alkenyl C3-C12, alkynyl C3-C10, cycloalkyl C3-C6 or the substituted derivatives of these groups and X, Y, Z, R1 and R2 are as described above, may be converted to the corresponding amide where A is CONH2 by reaction with ammonia under superatmospheric pressure at a temperature between about 25°C and 125°C. This reaction can be conducted in a protic solvent such as a lower alkanol or an aprotic solvent such as tetrahydrofuran, dioxane or the like. Likewise, using similar conditions but substituting hydroxylamine for ammonia in the above reaction yields the hydroxamic acid. These reactions may be graphically illustrated as follows:
Treatment of the thus prepared primary amide described above with titanium tetrachloride and triethylamine, preferably in the presence of an inert aprotic solvent, such as tetrahydrofuran yields the corresponding nitrile. The reaction is generally conducted under a blanket of inert gas, such as nitrogen, at a temperature between about 0°C and 10°C. The reaction may be illustrated as follows:
Preparation of the formula (VIII) N-substituted imidazolinone derivatives, wherein B is COR4 or SO2R5; and A is CH3, CN or COOR3; and W is 0; and R1, R2, R3, X, Y and Z are as described above, excepting that Y and Z cannot be alkylamino, hydroxy or hydroxyloweralkyl; can be achieved by reaction of the appropriately substituted formula (I) 2-(2-imidazolin-2-yl)pyridine with an excess of acyl halide, acyl anhydride, or sulfonyl halide, alone or in a solvent such as pyridine or toluene at an elevated temperature between about 50°C and 125°C. The reaction can be graphically illustrated as follows:
Reaction of either the formula (I) 2-(2-imidazolin-2-yl)pyridine or the formula (VIII) N-substituted imidazolinone derivatives, described and illustrated immediately above, wherein A is CH3, CN or COOR3 provided that R3 is as described above, excepting that it cannot be an unsaturated alkyl group, B is R4CO or R5SO2 and Y or Z cannot be alkylamino, alkylthio or dialkylamino; with an excess of m-chloroperbenzoic acid in the presence of an inert solvent such as methylene chloride, at refluxing temperature, yields the N-oxide corresponding to the pyridine derivative utilized as starting material. The reaction may be illustrated as follows:
Hydrolysis of the thus prepared N-oxide using a strong base such as sodium hydroxide in a lower alcohol yields the corresponding N-oxide in which B is H.
Advantageously, formula (I) esters in which B is hydrogen; W is oxygen and A is COOR3 wherein R3 is a saturated C1-C12 alkyl, C3-C6 cycloalkyl or benzyl substituent, and R1, R21 X, Y and Z are as defined above; can be prepared by reaction of the corresponding acid, i. e., where A is COOH, with an appropriate alcohol in the presence of a catalytic amount of a strong mineral acid such as hydrochloric acid, sulfuric acid or the like; at a temperature between about 50°C and 100°C. The reaction may be illustrated as follows:
The formula (I) acid as shown immediately above where A is COOH; B is hydrogen; W is oxygen and R1, R2, X, Y and Z are as defined above, is also readily converted to the corresponding methyl ester by reaction with diazomethane at a temperature between about 0°C and 25°C. The thus prepared methyl ester may then be reacted with an alkali metal alkoxide such as a sodium or potassium alkoxide, for convenience shown as R30Na, and an appropriate alcohol represented by the structure R30H, wherein R3 is Cl-C12 alkyl optionally substituted with one C1-C3 alkoxy, C3-C6 cycloalkyl, benzyloxy, furyl, phenyl, halophenyl, loweralkylphenyl, loweralkoxyphenyl, nitrophenyl or cyano; C3-C12 alkenyl optionally substituted with one or two C1-C3 alkoxy, phenyl or halogen groups; C3-C6 cycloalkyl optionally substituted with one or two C1-C3 alkyl groups or with C3-C10 alkynyl optionally substituted with one or two C1-C3 alkyl groups. The above reactions may be illustrated as follows:
Conversion of the above-identified formula (I) esters to their corresponding acid addition salts is readily achieved by treatment of said esters with strong acids, particularly strong mineral acids such as hydrochloric acid, sulfuric acid or hydrobromic acid.
Where the hydrohalide acid addition salts are desired, the formula (I) ester, wherein A is COOR3 and R3 is other than hydrogen or a salt-forming cation, and R1, R2, X, Y and Z are as described above, is dissolved in an organic solvent such as methylene chloride, chloroform, ether or the like. Addition of at least one equivalent of acid to the thus-prepared solution then yields the desired acid addition salt. The reaction may be illustrated as follows:
When the sulfuric acid salt of the ester is desired, the formula (I) ester is generally dissolved in a lower aliphatic alcohol such as methanol, ethanol, isopropanol or the like or mixtures of the above with water. Treatment of the mixture with at least one equivalent of sulfuric acid yields the sulfuric acid addition salt of the formula (I) ester.
In a further embodiment of the invention, the formula (I) compounds, wherein A is COOR3 and R3 is hydrogen and R1, R2, X, Y and Z, are as defined above, except that X, Y and Z cannot be N02 or halogen, can be prepared by hydrogenolysis of the benzyl ester of the imidazolinyl pyridine shown in formula (XV), wherein R1, R2, X, Y and Z, are as above-defined employing a palladium or platinum catalyst. In this reaction, the formula (XV) benzyl ester is dissolved or dispersed in an organic solvent, such as lower alcohol, an ether such as dioxane, tetrahydrofuran or the like, toluene or xylene. The catalyst, preferably palladium on a carbon carrier, is added to the mixture and the mixture heated to between 20°C and 50°C. The heated mixture is then treated with hydrogen gas to yield the desired acid. The reaction may be graphically illustrated as follows:
Alternatively, the formula (I) acids where A is COOH may be prepared by treatment of an aqueous solution of the formula (I) ester with a strong base. In practice the formula (I) ester is generally treated with one equivalent of base in aqueous solution, and the mixture heated to between 20°C and 50°C. The mixture is then cooled and adjusted to pH 6.5 to 7.5 and preferably pH 7, with a strong mineral acid. Such treatment yields the desired acid. The reaction can be illustrated as follows:
The formula (I) acids wherein A is COOH; B is hydrogen; W is oxygen and X, Y, Z, R1 and R2 are as described, can be prepared by reaction of the appropriately substituted formula (XVIII) imidazolinone with alkyl lithium, preferably in the presence of an inert solvent such as tetrahydrofuran under a blanket of nitrogen at a temperature between about -70°C and -80°C. The thus-formed mixture is then treated with hexamethylphosphoramide and carbon dioxide preferably in an inert solvent such as tetrahydrofuran, to yield the desired product. Where it is desirable to obtain the formula (I) pyridine derivatives in which A is CH3 and X, Y, Z, R1 and R2 are as stated above, the formula (XVIII) imidazolinone is treated in the same manner as described for the preparation of the acid, excepting that methyl iodide is substituted for the carbon dioxide. If dimethylformamide is substituted for the methyl iodide, the corresponding formyl derivatives are obtained. These reactions may be illustrated as follows:
Advantageously, the formula (I) acids may be converted to the formula (VII) 5-H-imidazo[1',2':1,2)-pyrrolo[3,4-b]pyridine-3(2H),5-diones by reaction with dicyclohexylcarbodiimide (DCC). The reaction is preferably conducted using approximately an equimolar amount of the carbodiimide in the presence of a chlorinated hydrocarbon solvent at a temperature between about 20°C to 32°C. The reaction may be graphically illustrated as follows:
The formula (VII) 5H-imidazo[1',2':1,2]pyrrolo-[3,4-b]pyridine-3(2H),5-diones are isomers of the formula (III) imidazopyrrolopyridinediones referred to above, and are especially useful in the preparation of a variety of the formula (I) 2-(2-imidazolin-2-yl)-pyridine derivatives of the present invention, as will become apparent from the following discussion.
In practice, it is found that the formula (VII) 3(2H),5-diones can be reacted with at least one equivalent of an appropriate formula (V) R30H alcohol in the presence of triethylamine as catalyst to yield the formula (I) pyridine ester corresponding to the alcohol used. The reaction is preferably conducted at a temperature between about 20°C and 50°C in the presence of an inert aprotic solvent, such as tetrahydrofuran, dioxane or the like. The reaction may be illustrated as follows:
The formula (VII) 3(2H),5-diones are also readily converted to the formula (Ib) 2-(2-imidazolin-2-yl)-pyridine derivatives wherein R1, R2' X, Y and Z, are as described above; W is oxygen; B is hydrogen, and A is acetyl, benzoyl, trimethylphosphonoacetate or hydroxymethyl; by reaction thereof with methyl magnesium bromide, phenyl lithium, sodium trimethyl phosphonoacetate or sodium borohydride; respectively. The methyl magnesium bromide, phenyl lithium and sodium trimethylphosphonoacetate reactions are preferably carried out at a temperature between about -50°C and -80°C, in the presence of an inert solvent such as tetrahydrofuran or dioxane under a blanket of inert gas, such as nitrogen. Reaction of the above-said formula (VII) diones with sodium borohydride is relatively mild. The reaction does not require a blanket of inert gas and can be conducted at temperatures between about -10°C and +15°C.
Reaction of the formula (VII) diones with at least one equivalent of acetone oxime yields the acetone oxime ester of the formula (I) 2-(2-imidazolin-2-yl)pyridine where A is COON=C(CH3)2, B is hydrogen and R1, R2, X, Y and Z, are as described for formula (I) pyridine derivatives. The above reaction is generally conducted in the presence of an inert organic solvent such as toluene, benzene, xylene or the like at a temperature between about 40°C and 80°C.
The above reactions are graphically illustrated below:
Formula (I) compounds, wherein A is COOR3 and R3 represents a salt-forming cation such as an alkali metal, alkaline earth metal, ammonium or aliphatic ammonium and Rl, R2, X, Y and Z are as described above, can be prepared by dissolving the formula (I) 2-(2-imidazolin-2-yl)pyridine acid in an appropriate solvent and, thereafter, treating the solution of the acid with one equivalent of salt-forming cation. For compounds in which the salt-forming cation is an inorganic salt such as sodium, potassium, calcium, barium or the like, the formula (I) acid may be dissolved or dispersed in water or a lower alcohol or mixtures thereof. One equivalent of the salt-forming cation generally in the form of the hydroxide, carbonate, bicarbonate or the like, but preferably as the hydroxide, is admixed with the solution of the formula (I) acid. After several minutes, the formula (I) compound, wherein R3 is the inorganic salt-forming cation, generally precipitates and can be recovered from the mixture by either filtration or through azeotropic distillation with an organic solvent such as dioxane.
To prepare the formula (I) compound in which A is COOR3 and R3 is ammonium or organic ammonium, the formula (I) acid is dissolved or dispersed in an organic solvent such as dioxane, tetrahydrofuran or the like, and the mixture treated with one equivalent of ammonia or the amine or the tetralkylammonium hydroxide. Among the amines which may be used in the above-said reaction are: methylamine, ethylamine, n-propylamine, isopropylamine, n-butylamine, isobutylamine, sec-butylamine, n-amylamine, iso-amylamine, hexylamine,. heptylamine, octylamine, nonylamine, decylamine, undecylamine, dodecylamine, tridecylamine, tetradecylamine, pentadecylamine, hexadecylamine, hepta- decylamine, octadecylamine, methylethylamine, methyl- isopropylamine, methylhexylamine, methylnonylamine, methylpentadecylamine, methyloctadecylamine, ethylbutylamine, ethylheptylamine, ethyloctylamine, hexylheptyl- amine, hexyloctylamine, dimethylamine, diethylamine, di-n-propylamine, diisopropylamine, di-n-amylamine, diisoamylamine, dihexylamine, diheptylamine, dioctylamine, trimethylamine, triethylamine, tri-n-propylamine, triisopropylamine, tri-n-butylamine, triisobutylamine, tri-sec-butylamine, tri-n-amylamine, ethanolamine, n-propanolamine, isopropanolamine, diethanolamine, N,N- I diethylethanolamine, N-ethylpropanolamine, N-butylethanolamine, allylamine, n-utenyl-2-amine, n-pentenyl-2-amine, 2,3- dimethylbutenyl-2-amine, dibutenyl-2-amine, n-hexenyl- 2-amine, propylenediamine, tallowamine, cyclopentylamine, cyclohexylamine, dicyclohexylamine, piperidine, morpholine, and pyrrolidine. Among tetralkylammonium hydroxides contemplated methyl, tetraethyl, trimethylbenzylammonium hydroxides. In practice, after several minutes, the ammonium or organic ammonium salt precipitates and can be separated from the solution by any convenient means, as by filtration or centrifugation. Additionally, the reaction mixture may be concentrated, and the remaining solvent removed with hexane, and the residue then dried to recover the ammonium or organic ammonium formula (I) salt. The above reactions may be graphically illustrated as follows:
When R1 and R2 represent different substituents, the carbon to which R1 and R2 are attached is an asymmetric center, and the products (as well as their intermediates) exist in d- and I- forms as well as d1-forms.
It should also be understood that the 2-(2-imi- dazolin-2-yl)pyridines and quinolines represented by formula (I) in which B = H may be tautomeric, while for convenience, they are depicted by a single structure identified as formula (I), they may exist in either of the isomeric forms illustrated as follows:
One general method for the preparation of the formula (I) compounds involves the reaction of a quinolinic anhydride of formula (XVI) hereinbelow, with an appropriately substituted a-aminocarbonitrile of formula (XVII), hereinbelow, to yield a mixture of the monoamides of quinolinic acid of formula (IX) and formula (X).
This reaction is carried out at a temperature between about 20°C and 70°C and preferably between about 35°C and 40°C in an inert solvent, such as tetrahydrofuran, methylene chloride, ether, chloroform, toluene or the like. The thus-formed acids are then cyclized to the corresponding pyrrolopyridine acetonitrile, depicted by formula (XI), by heating the reaction mixture with an excess of acetic anhydride in the presence of a catalytic amount of sodium acetate or potassium acetate.
In general, the above reaction is carried out by treating the reaction mixture with acetic anhydride, acetyl chloride, thionyl chloride or the like and heating said mixture to a temperature between about 20°C and 100°C. Hydration of the thus-formed pyrrolopyridine acetonitrile formula (XI) is carried out by treating said acetonitrile with a strong acid such as sulfuric acid. This reaction yields the formula (XII) pyrrolopyridine acetamide. Although the addition of a non-miscible solvent such as methylene chloride, chloroform or the like is not essential to the conduct of the above described reaction, addition of such a solvent to the reaction mixture is generally preferred. Said reaction is usually carried out at a temperature between about 10°C to 70°C.
The cyclization of the formula (XII), hereinbelow; pyrrolopyridine acetamide yields the tricyclic formula (III) imidazopyrrolopyridinediones which are intermediates for the imidazolinyl nicotinic acids and esters of the present invention referred to above and represented by formula (Ib).
The product of this reaction is predominantly the desired imidazopyrrolopyridinedione (85%) together with the isomer of formula (IIIa). Mixtures of this ratio of the two isomers generally give substantially isomerically pure nicotinate product.
The cyclization reaction is preferably conducted at a temperature of from 800C to 150°C in the presence of a base such as sodium or potassium hydride or an acid such as an aromatic sulfonic acid and a solvent which will form an azeotropic mixture with water, permitting virtually immediate removal thereof from the reaction mixture as it is formed. Among the solvents which may be employed are toluene, benzene, xylenes and cyclohexane. Bases which may be used include alkali metal hydroxides, alkali metal hydrides, alkali metal oxides, tertiary amines such as diisopropyl ethylamine, 1,5-diazabicyclo[3.4]nonene-5,1,5-diazobicyclo[5.4.0]-undecene-5,l,4-diazabicyclo[2.2.2]octane, tetramethylguanidine, potassium fluoride and quaternary ammonium hydroxide, such as trimethylbenzyl ammonium hydroxide and strongly basic ion exchange resins.
Finally, acidic reagents which can be employed herein include aromatic sulfonic acids, such as p-toluenesulfonic acid, B-naphthalenesulfonic acid, naphthalenedisulfonic acid, and the like.
The mixture of compounds of formula (III) and formula (IIIa) is then converted to formula (Ib), as discussed above, with an alkali metal alkoxide and alcohol.
The above reactions are graphically illustrated on Flow Diagram I below, when X, Y, Z, R1, R2 and R3 are as defined above.
Another general method for the preparation of the formula (I) pyridine derivatives involves the reaction of a quinolinic anhydride of formula (XVI), with an appropriately substituted a-aminocarboxylic acid such as a-methylvaline represented by formula (XIX), preferably in a ketonic solvent such as acetone under a blanket of nitrogen to yield an isomeric mixture of the formula (XX) and formula (XXI) acids. The mixture is then treated with acetic anhydride and a catalytic amount of sodium acetate at an elevated temperature to give the dihydrodioxopyrrolopyridine acid formula (XXII). Reaction of the thus-formed acid with a thionyl halide, such as thionyl chloride or thionyl bromide, in the presence of an organic solvent such as toluene, xylene, benzene or the like, at an elevated temperature, . i.e., 800C to 150°C, gives the formula (XXIII) acid halide corresponding to the formula (XXII) acid. Treatment of this acid halide with excess ammonia then yields the formula (IV) dihydrodioxopyrrolopyridine acetamide. The reaction is preferably carried out in the presence of an aprotic solvent.
Reaction of the formula (IV) acetamide with 1,8-diazabicyclo[5,4,0]undec-7-ene in an inert organic solvent such as toluene or xylene at an elevated temperature between about 80°C and 125°C gives the formula (III) imidazopyrrolopyridinedione which may be heated with morpholine or an appropriate NH2R6 amine to yield the 2-(2-imidazolin-2-yl)nicotinamides. These reactions are illustrated as Flow Diagram II.
In another general procedure, the 2-(2-imidazolin-2-yl)pyridine acids and esters of formula (I), can be prepared by reacting the 2-carboalkoxynicotinoyl chloride of formula (XIV), hereinbelow, preferably as the methyl ester and preferably in the form of the hydrochloride salt, with the appropriate aminocarboxamide depicted by formula (XIII). The reaction yields the carbamoyl picolinate, formula (XV), and is preferably carried out in an inert blanket of gas such as nitrogen. The reaction mixture is generally maintained at a temperature below 30°C during the reaction period.
The thus-formed carbamoyl picolinate, formula (XV), hereinbelow, can then be dispersed in an inert non-protic solvent such as xylene or toluene and heated to about 50°C to 130oC with l,5-diazabicyclo-[5.4.0]undec-5-ene. The reaction yields a mixture of the formula (III) and formula (IIIa), imidazopyrrolopyridinedione isomers which can be used without separation in the following reaction, wherein the reaction mixture is treated with an alkali metal alkoxide, in the presence of an alcohol to yield a mixture of the imidazolinyl nicotinate and the imidazolinyl picolinate. The desired formula (Ib) nicotinate can be readily separated from the picolinate by neutralizing the reaction mixture, preferably with glacial acetic acid, concentrating the neutralized solution and chromatographing the resulting residue on silica gel in ether.
Conversion of the imidazolinyl nicotinate esters to the corresponding acids or acid addition salts can be readily achieved by the methods previously described. Similarly, the imidazolinyl nicotinic acids can be converted to the corresponding alkali metal, ammonium, or organic ammonium salts by the methods previously described.
Preparation of the formula (I) acids and esters, by the route described above, is graphically illustrated in Flow Diagram III below.
Advantageously, many of the formula (II) 2-(2-imidazolin-2-yl)quinoline derivatives of the present invention can be prepared by the procedures described above for the preparation of the formula (I) 2-(2-imidazolin-2-yl)pyridine compounds. For example, the formula (XXXVI) 2-(2-imidazolin-2-yl)quinolinecarboxylate esters, wherein R3 represents a substituent other than hydrogen or a salt-forming cation, and R1, R2, X, L, M, Q and R7 are as described above, can be prepared by reacting the formula (XXXVII) dione, with an appropriate alcohol and alkali metal alkoxide at a temperature between about 20°C and 50°C. In these reactions, as ; with similar reactions in which the formula (I) pyridines are prepared, the alcohol can function both as a reactant and a solvent. As such, a secondary solvent is not required, but can be employed if so desired. When a secondary solvent is used, it is preferable to employ a non-protic solvent such as tetrahydrofuran or dioxane. The reaction may be illustrated as follows:
The formula (XXXVI) 2-(2-imidazolin-2-yl)quinolinecarboxylate esters can also be prepared from a formula (XXXVIII) dioxopyrroloquinoline acetamide, wherein R1, R2, X, L, M, Q and R7 are as described above, by cyclization thereof with a strong base, such as 1,5- diazabicyclo[5.4.0]undec-5-ene (DBU) in the presence of an inert organic solvent such as xylene or toluene to give the crude imidazopyrroloquinolinedione of formula (XXXVII). The reaction mixture is heated to a temperature between 100oC and 150°C, and water removed from the reaction mixture using a Dean-Stark water separator. At least one equivalent of alcohol R3OH represented by formula (V); wherein R3 is a substituent as described above, but excluding hydrogen and salt-forming cations, is then added to the reaction mixture, and the thus prepared mixture heated to reflux at a temperature between 100°C and 150°C to yield the formula (XXXVI) ester. The reaction may be graphically illustrated as follows:
The formula (XXXVI) 2-(2-imidazolin-2-yl)quinolinecarboxylate esters can also be prepared by cyclization of a carbamoyl quinolinecarboxylate ester, represented by formula (XXXIX), with phosphorus pentachloride at an elevated temperature between about 600C and 100°C. The reaction is generally conducted in the presence of an inert organic solvent, such as toluene or benzene, and yields the hydrochloride salt of the formula (XXXVI) 2-(2-imidazolin-2-yl)quinolinecarboxylate ester. Treatment of the thus formed hydrohalide salt with base, , such as sodium or potassium carbonate, then yields the formula (XXXVI) 2-(2-imidazolin-2-yl)quinolinecarboxylate ester. The carbamoyl quinolinecarboxylate ester, formula (XXXIX), used in the above reaction may be shown as follows:
The 2-(2-imidazolin-2-yl)quinolinecarboxylate esters of formula (XXXVI) are also formed by cylization of the carbamoyl quinolinecarboxylate esters represented by formula (XXXIX), and having the structure:
The formula (XXXVI) quinoline ester in which R3 is as described above, but excluding hydrogen or salt-forming cations, and R1, R2, X, L, M, Q and R7 are as described above, is readily converted to its corresponding acid addition salt by reaction of said ester with at least one equivalent of a strong acid. Strong mineral acids such as hydrochloric acid, sulfuric acid, and hydrobromic acid are employed although organic acids may also be used. In practice, it is also found that the reaction proceeds most satisfactorily when conducted in the presence of an inert organic solvent such as ether, chloroform, methylene, chloride, or mixtures I thereof. Sulfuric acid salts are generally prepared by this procedure, but substituting a lower aliphatic alcohol for the above-mentioned solvents.
Preparation of the formula (II) 2-(2-imidazolin-2-yl)quinoline derivatives wherein A is COOH: B is hydrogen; W is oxygen and R1, R2, X, L, M, Q and R7 are as defined, with the proviso that X, L, M, Q and R7 are not halogen or nitro, can be achieved by hydrogenolysis of the benzyl ester of the formula (XXXVI) 2-(2-imidazolin-2-yl)quinolinecarboxylate. The reaction involves dispersion of the benzyl ester in an organic solvent, such as described above for the hydrogenolysis of the formula (XV) benzyl ester in the 2-(2-imidazolin-2-yl)pyridine series, and treatment of the thus-prepared reaction mixture with hydrogen gas in the presence of a catalyst such as palladium or platinum on a carbon carrier. The hydrogenolysis is generally carried out at a temperature between about 20°C and 50°C.
The acids of the formula (II) 2-(2-imidazolin-2-yl)quinoline derivatives in which A is COOH; B is hydrogen; W is oxygen and R1, R2, X, L, M, Q and R7 are as described above, are also prepared by reaction of a formula (XXXVI) ester, in which R3 is a substituent as described above, but excluding hydrogen and salt-forming cations and R1, R2, X, L, M, Q and R7 are as described, with at least one equivalent of strong aqueous base, such as an aqueous solution of an alkali metal hydroxide, at a temperature between 20°C and 50°C. The mixture is cooled and then adjusted to pH 6.5 to 7.5 with a strong mineral acid. Such treatment yields the desired acid.
Preparation of the formula (II) 2-(2-imidazolin-2-yl)quinoline derivatives in which A is COOR3; R3 is a salt-forming cation; B is hydrogen; W is oxygen and R1, R2, L, M, Q and R7 are as described above, can be prepared by dissolving an acid, represented by formula (II) wherein A is COOH; B is hydrogen; W is oxygen and R1, R2, L, M, Q and R7 are as described above, in an appropriate solvent and treating the thus prepared mixture with at least one equivalent of a salt-forming cation. The reaction is essentially the same as described for the preparation of the formula (I) pyridines in which A is COOR3 and R3 is a salt-forming cation.
It should also be understood that the imidazolinyl quinolinecarboxylic acids and esters depicted by formula (II) in which B is H may be tautomeric.
It should also be understood that when R1 and R2 represent different substituents on the formula (II) 2-(2-imidazolin-2-yl)quinoline derivatives and the formula (XXXVII) imidazopyrroloquinolinediones, the carbon to which R1 and R2 are attached is an asymmetric center, and the products (as well as their intermediates) exist in d- and 1- forms as well as dl forms.
Cyclization of the formula (XXXVIII) imidazopyrrolo- quinoline acetamide yields the tetracyclic formula (XXXVII) and formula (XXXVIIa) imidazopyrroloquinolinediones which are intermediates for the formula (II) 2-(2-, imidazolin-2-yl)quinolinecarboxylate acids and esters.
The product of this reaction is predominately the desired imidazopyrroloquinolinedione together with a small amount of the formula (XXXVIIa) isomer. Treatment of the isomeric mixture with an alkali metal alkoxide gives substantially isomerically pure quinolinecarboxylate product.
The cyclization reaction is preferably conducted at a temperature of from 80°C to 150°C in the presence of a base such as sodium or potassium hydride or an acid such as an aromatic sulfonic acid and a solvent which will form an azeotropic mixture with water, permitting virtually -immediate removal thereof from the reaction mixture as it is formed. Among the solvents which may be employed are toluene, benzene, xylenes and cyclohexane. Bases which may be used include alkali metal hydroxides, alkali metal hydrides, alkali metal oxides, tertiary amines such as diisopropyl ethylamine, 1,5-diazabicyclo-[3.4]nonene-5,1,5-diazobicyclo[5.4.0]undecene-5,1,4-diazabicyclo[2.2.2]octane, tetramethylguanidine, potassium fluoride and quaternary ammonium hydroxide, such as trimethylbenzyl ammonium hydroxide and strongly basic ion exchange resins.
Finally, acidic reagents which can be employed herein include aromatic sulfonic acids, such as p-toluenesulfonic acid, β-naphthalenesulfonic acid, naphthalenedisulfonic acid, and the like.
The reactions are graphically illustrated in Flow Diagram IV.
Several routes are employed to prepare pyrroloquinoline acetamides represented by formula (XXXVIII). Preferably hydration of the pyrroloquinoline acetonitrile of formula (XXXX) is accomplished by treatment with a strong acid such as sulfuric acid. This reaction yields the formula (XXXVIII) pyrroloquinoline acetamide. Although the addition of a non-miscible solvent such as methylene chloride, chloroform or the like is not essential to the conduct of the above described reaction, addition of such a solvent to the reaction mixture may be preferred. Said reaction is usually carried out at a temperature between 10°C to 70°C.
Alternatively, the pyrroloquinoline acetamide of formula (XXXVIII) may be obtained by the Diels-Alder cycloaddition reaction between substituted anthranils of formula (XXXXI) and the dioxopyrroline acetamide of formula (XXXXII). These reactions are carried out in a wide temperature range: below 130°C significant amounts of the intermediate aldehyde of formula (XXXXIII) are obtained, but at 130°C - 200°C the pyrroloquinoline acetamide of formula (XXXVIII) is formed. Alternatively, the intermediate aldehyde of formula (XXXXIII) may be isolated and cyclized in refluxing xylene in the presence of an acid catalst, such as E-toluene sulfonic acid. This reaction yields the desired formula (XXXVIII) pyrroloquinoline acetamide. These reactions may be. graphically illustrated as shown below on Flow Diagram V.
A variety of routes may also be employed to obtain the formula (XXXX) pyrroloquinoline acetonitriles depending on the nature of the L, M, Q and R7 substituents.
The formula (XXXX) pyrroloquinoline acetonitriles may be prepared by reacting the appropriately substituted anhydride (XXXXIV) with an appropriately substituted α-aminocarbonitrile of formula (XVII), to yield a mixture of the monoamides of the formula (XXXXVa) and formula (XXXXV b) acids.
This-reaction is carried out at a temperature between 20°C and 70°C and preferably between about 35°C and 40°C in an inert solvent, such as tetrahydrofuran, methylene chloride, ether, chloroform, toluene or the like. The thus-formed acids are then cyclized to the corresponding pyrroloquinoline acetonitrile, depicted by formula (XXXX). This is accomplished by heating the reaction mixture to a temperature between about 75°C and 150°C with an excess of acetic anhydride in the presence of a catalytic amount of sodium acetate or potassium acetate.
In general, the above reaction is carried out by treating the reaction mixture with acetic anhydride, acetyl chloride, thionyl chloride or the like, and heating said mixture to a temperature between about 20°C and 100°C. The above reactions are illustrated graphically on Flow Diagram VI below, wherein R1, R2' X, L, M, Q and R7, are as defined above.
A preferred route to pyrroloquinoline acetonitrile of formula (XXXX) is the Diels-Alder thermal cycloaddition reaction of an appropriately substituted maleimide of formula (XXXXVI) with an appropriately substituted anthranil. In this route X must be hydrogen.
The outcome of this reaction is dependent upon the reaction temperature. Intermediate of formula (XXXXVII) is obtained at -55°C. As the reaction mixture is heated further, to temperatures between 55°C and 130°C, the intermediate of formula (XXXXIII) aldehyde is obtained., If the reaction is conducted in the presence of an aprotic solvent such as o-dichlorobenzene, and the reaction mixture heated to between 140°C and 200°C, the reaction yields the pyrroloquinoline acetonitrile of formula (XXXX). The reaction is illustrated on Flow Diagram VII below.
The above route is particularly effective when L, M, Q and R7 groups are electronegative, e.g., halogens, nitro, CF3, SO2CH3, CN.
A variation of this procedure involves the reaction of an o-aminoacetal of formula (XXXXVIII) with an appropriately substituted formula (XXXXX) maleimide or dioxopyrroline acetamide, in the presence of an aprotic solvent such as xylene or toluene at a temperature between about 50°C and 130°C. These reactions are graphically illustrated below.
Yet a further variation, useful for synthesis from anilines bearing electron donor substitution in the L, M, Q and R7, positions or one halogen or CF3 function, is the interaction of o-alkyl or arylthiomethylanilines formula (XXXXIX) with a bromomaleimide of formula (XXXXX) to give the maleimide of formula (XXXXXI) which is oxidized to the maleimide of formula (XXXXXII). The maleimide of formula (XXXXXII) is then cyclized in an acid catalyzed reaction to yield the pyrroloquinoline acetonitrile of formula (XXXX). The reactions are graphically illustrated below. R1, R2, L, M, Q and R7 are as defined above.
Compounds of formula (XXXX) bearing electron donor substitution on the L, M, Q and R7 positions, such as alkyl, alkoxy, alkylthio, dialkylamino, hydroxy and a single halogen may be prepared by reaction of an appropriately substituted o-aminobenzyl alcohol of formula (XXXXXIII) or anthranilic acid of formula (XXXXXIV) with a bromo(or chloro)maleimide of formula (XXXXX).
This reaction is conducted in the presence of a protic solvent, such as isopropyl or t-butylalcohol at 0°C to 30°C to yield respectively, the hydroxy- methylanilinomaleimide of formula (XXXXXV) or dioxo- pyrrolinyl anthranilic acid of formula (XXXXXVI). A variety of base acceptors can be employed in the above reactions, e.g., alkaline earth metal hydroxides, such as Ba(OH)2' BaO or sodium acetate. However, the reactions in many instances proceed satisfactorily without the aid of the base acceptor.
Oxidation of the formula (XXXXXV) alcohol to the formula (XXXXIII) aldehyde may be accomplished using a wide variety of oxidizing agents exemplified by pyridinium- chlorochromate in methylene chloride or activated manganese dioxide in t-butanol. Cyclization of the aldehyde of formula (XXXXIII) to the pyrroloquinoline acetonitrile of formula (XXXX) is achieved by one of the methods described above, such as heating said aldehyde to between 140°C and 200°C in the presence of an aprotic solvent.
Cyclization of the o-anilinocarboxylic acid of formula (XXXXXVI) to the acetoxyquinoline of formula (XXXXXVII) is accomplished with acetic anhydride, triethylamine and 4-dimethylaminopyridine at ambient temperatures. Mild reductive elimination gives the pyrroloquinoline acetonitrile of formula (XXXX). Hydrolysis in warm aqueous acetic acid gives formula (XXXX) where X = OH, further reaction with phosphorus oxychloride and pyridine, affords compounds where X is chlorine. These reactions are illustrated in Flow Diagram VIII below.
Another route to the 2-(2-imidazolin-2-yl)quinoline compounds of formula (II), particularly useful in synthe-. sizing analogs in which the A group is varied, employs the 2-(2-imidazolin-2-yl)quinoline of formula (XXXXXIX). This intermediate is prepared from quinolinecarboxylic acid of formula (XXXXXVIII) which is converted to the acid chloride or anhydride and then reacted with either an appropriately substituted a-aminocarbonitrile of formula (XVII) to give the formula (XXXXXX) nitrile, or with the aminoamide of formula (XXXXXXI) to give the carboxamido- amide of formula (XXXXXXII). Cyclization of said carbox- amidoamide is accomplished by the previously discussed procedures, although cyclization with sodium hydride in the presence of xylene is preferred. Introduction of a variety of groups, A, is made possible by treatment of the 2-(2-imidazolin-2-yl)quinoline of formula (XXXXXIX) with metallating reagents. Most organo metallic agents have some effect when two moles are used with the formation of a dianion, but the yield and reaction composition is dependent upon the nature of the organometallic reagent, reaction solvent, reaction temperature and the electrophile used to quench the reaction. In practice organometallic reagents such as alkyl lithiums are preferred'and anion formation is obtained with methyl, n-butyl, sec-butyl and tert-butyl lithium. Phenyl lithium and lithium diisopropylamide may also be employed. Solvents must be aprotic and diethylether is preferred. Reaction temperatures employed to form the dianion range from -78°C to 0°C with -30°C to -10°C preferred. Quenching with an electrophile is usually accomplished at -780C to +20°C. If necessary, this is followed by acid treatment. All reactions are run under an inert atmosphere. Examples of reactive electrophiles include: CO2, ClCO2CH3, (CH3)2NCHO, CH3HCO, C6H5CHO, CH3I. The corresponding values for A in formula (IIa) are COOH, COOCH3, CHO, CH(OH)CH3, CH(OH)C6H5, CH3. Furthermore, following the quenching of the electrophile, the products may be modified. Thus the aldehyde (A=CHO) prepared from a DMF quench, reacts with hydroxylamine to afford an oxime. The above route is also useful for the synthesis of A=COOH compounds by treatment of the dianion with carbon dioxide.
Not all X, L, M, Q and R7, substituents are compatible with this organometallic process. Thus, if the above mentioned substituents are Br, I or sometimes fluorine, competitive loss of these groups occurs as well as replacement of the 3-proton of quinoline. Chlorine, however, is compatible with this process. When L, M, Q or R7, is methoxy, competitive anion formation can occur ortho to the methoxy group.
The above-described reactions are graphically illustrated on Flow Diagram IX below.
Several functional groups, A, are prepared by an alternative approach. Thus reaction of the imidazopyrroloquinolinedione of formula (XXXVII) with R3OM+ affords the 2-(2-imidazolin-2-yl)quinoline esters of formula (XXXVI). In the case of formula (II) compounds in which A is the CONHCH2CH20H group, this may be cyclized to the oxazoline group via reaction of the above-identified compound with triethylphosphite in refluxing xylene. Formula (II) derivatives, in which A is -CONH2, may be converted to the corresponding cyano derivative, i.e., A=CN, by dehydration of the -CONH2 function.
The majority of compounds containing L, M, Q and , R71 substituents may be prepared by the procedures previously outlined; however, amino, alkylamino, and dialkylamino compounds are conveniently formed by reductive alkylation of the appropriate nitro substituent in the L, M, Q or R7 position. Alkylsulfonyl compounds are most readily formed by mild oxidation of the alkylsulfonyl group in the L, M, Q or R7 position at 0°C - 20°C using m-chloroperbenzoic acid. Under these conditions, N- oxidation of the quinoline nitrogen is minimized.
Oxidation of the quinoline to the N-oxide can be accomplished by prior N-protection of the imidazolone ring with, e.g., a COCH3 group; N-oxidation can now be accomplished by peracetic acid or trifluoroperacetic acid, at elevated temperatures, if necessary.
The formula (I) 2-(2-imidazolin-2-yl)pyridine and quinoline compounds as hereinabove defined are surprisingly effective for inducing and/or accelerating the maturation of cereal crops such as wheat, barley, rye, oats, . rice and corn, without adversely affecting the quality of seeds and the yields thereof, when applied to said plants and/or to the soil containing the seeds of said plants in amounts of from 0.0001 kg/ha to 0.01 kg/ha and preferably 0.0002 kg/ha to 0.005 kg/ha.
The method of using formula (I) compounds to accelerate and/or induce maturing, and thus allow the earlier than usual harvesting of cereal crops is, or could be, quite important to the users thereof. Thus, for instance, in any given location the so treated cereal crops may be harvested well.ahead of their usual time of harvest, and the land so freed is immediately available for the planting of a second crop which otherwise would not have sufficient time to grow and reach a stage or, size suitable to harvest.
Such a method, would also make it possible to allow a cereal crop to be harvested on time even though its planting may have been delayed due to adverse weather conditions.
Furthermore, the use of formula (I) compounds to accelerate the maturing of cereal crops may make possible the planting and harvesting of such crops in certain regions of the world where the growing season is normally of shorter duration than the time needed to allow said crops to mature.
Interestingly, formula (I) 2-(2-imidazolin-2-yl)-pyridines and quinolines, when applied to legumes such as soybeans, beans, peas and lentils and/or to soil containing the seeds of said plants, in amounts of from about 0.0001 kg/ha to about 0.01 kg/ha and preferably 0.002 kg/ha to 0.005 kg/ha, will improve the axillary branching and tillering of said plants. The thus- treated legumes also show increased flowering, increased pod set, and increased yields.
The formula (I) compounds, as defined above, are also quite effective for inducing branching and increasing flowering of herbaceous ornamentals such as colei, dahlias, tulips, daffodils, crocuses, crysanthemums and roses when applied to said plants and/or to the soil containing the seeds or other propagating organs of said plants in amounts of from about 0.0001 kg/ha to 0.01 kg/ha preferably 0.0002 kg/ha to 0.005 kg/ha.
The compounds of formula (I), (Ia) and (II), are disclosed and claimed as herbicides in copending application Serial NO. , filed , which is a continuation in part of application Serial No. 155,909, filed June 2, 1980.
Advantageously, the compounds of this invention can be formulated as solid or liquid compositions which may be dispersed in a liquid or solid diluent for application to vegetative matter.
Since the formula (I) derivatives, wherein R3 is a salt forming cation, are water soluble, these compounds can simply be dispersed in water and applied as a dilute aqueous spray to the foliage of agronomic and horticultural plants or to soil containing the seeds or other propagating organs thereof. These salts also lend themselves to formulation as flowable concentrates.
The 2-(a-imidazolin-2-yl)pyridines and quinolines of formula (I) can be formulated as wettable powders, flowable concentrates, emusifiable concentrates and granular formulations.
A typical flowable liquid can be prepared by admixing about 40% by weight of the active ingredient with about 2% by weight of a gelling agent such as bentonite, 3% by weight of a dispersing agent such as sodium lignosulfonate, 1% by weight of polyethylene glycol and 54% by weight of water.
A typical emulsifiable concentrate can be prepared by dissolving about 25% by weight of the active ingredient in about 65% by weight of N-methylpyrrolidone, isophorone, butyl cellosolve, methylacetate or the like and dispersing therein about 10% by weight of a nonionic surfactant such as alkylphenoxypolyethoxy alcohol. This concentrate is dispersed in water for application as a liquid spray.
When the above compounds are to be used where soil treatments are involved, the compounds may be prepared and applied as granular products. Preparation of the granular product can be achieved by dissolving the active compound in a solvent such as methylene chloride, N-methylpyrrolidone or the like and spraying the thus prepared solution on a granular carrier such as corncob grits, sand, attapulgite, kaolin or the like.
Wettable powders can be prepared by grinding together about 20% to 45% by weight of a finely divided carrier such as kaolin, bentonite, diatomaceous earth, attapulgite, or the like, 45% to 80% by weight of the active compound, 2% to 5% by weight of a dispersing agent such as sodium lignosulfonate, and 2% to 5% by weight of a nonionic surfactant, such as octylphenoxy polyethoxy ethanol, nonylphenoxy polyethoxy ethanol or the like.
A typical flowable liquid can be prepared by admixing about 40% by weight of the active ingredient with about 2% by weight of a gelling agent such as bentonite, 3% by weight of a dispersing agent such as sodium lignosulfonate, 1% by weight of polyethylene glycol and 54% by weight of water.
A typical emulsifiable concentrate can be prepared by dissolving about 5% to 25% by weight of the active ingredient in about 65% to 90% by weight of N-methylpyrrolidone, isophorone, butyl cellosolve, methylacetate or the like and dispersing therein about 5% to 10% by weight of a nonionic surfactant such as an alkylphenoxy polyethoxy alcohol. This concentrate is dispersed in water for application as a liquid spray.
When the compounds of the invention are to be used
where soil treatents are involved, the compounds may be prepared and applied as granular products. Preparation of the granular product can be achieved by dissolving the active compound in a solvent such as methylene chloride, N-methylpyrrolidone or the like and spraying the thus prepared solution on a granular carrier such as corncob grits, sand, attapulgite, kaolin or the like.
The granular product thus prepared generally comprises about 3% to 20% by weight of the active ingredient and about 97% to 80% by weight of the granular carrier.
A composition for the treatment of a one hectare plot, to increase the crop yield of cereal grains or legumes grown thereon, comprises from 100 to 500 liters of water; from 0.01 to 0.001 kg of a compound having the structure:
In order to facilitate a further understanding of the invention, the following examples are presented primarily for the purpose of illustrating certain more specific details thereof. The invention is not be be deemed limited thereby except as defined in the claims. Unless otherwise noted, all parts are by weight.
To a stirred solution containing 212 g quinolinic anhydride in 950 ml methylene chloride is added at a moderate rate 167 g of 2-amino-2,3-dimethylbutyronitrile. The mixture had reached the boiling point of the solution after about one quarter of the aminonitrile had been added and the rate of addition is adjusted to maintain this temperature. After the addition the solution is heated under reflux for a further 4 hours. The solution is cooled, filtered and concentrated to a thick oil. This oil is dissolved in 950 ml acetic anhydride, 6 g anhydrous sodium acetate added and the mixture distilled until the vapor temperature reached 1180C when the heating was continued under reflux for 3 hours. The mixture is concentrated in vacuo the residue dissolved in 500 ml toluene and again concentrated. This is repeated. The residue is slurried with a mixture of ether and hexane and the crude product which crystallizes collected (349 g). This is dissolved in 700 ml methylene chloride and filtered through a column containing 700 g silica gel and the product eluted with methylene chloride. Concentration of the eluant gave 258 g of the desired product. An analytically pure sample with mp 95-96°C can be obtained by the recrystallization of the product from ether-methylene chloride.
Using the appropriate amino nitrile and quinolinic anhydride in the above procedure, the following pyrrolopyridines are prepared:
To 330 ml concentrated sulfuric acid is added portion wise with thorough stirring 298 g finely divided nitrile so that the temperature did not go about 720C. After the addition the temperature is adjusted to 60-65oC and maintained there for 1 1/2 hours. The mixture is cooled, quenched with ice and finally diluted to approximately 4 liters. After adding 454 g sodium acetate and cooling at 0°C for 2 hours the mixture is filtered, the solids collected and washed twice with 500 ml water containing sodium acetate followed by water to remove all the sulfuric acid. The solid is dried to give 289 g of product, mp 176-178°C. Material made in a similar way and analytically pure had mp 188-1900C.
Employing the appropriate pyrrolopyridine- acetonitrile in the above procedure, the following pyrrolopyridineacetamides are prepared.
A mixture of 50 g amide and 450 ml toluene is heated under a Dean-Stark water separator to remove traces of water. To the cooled mixture is added 10.1 g of a 50% suspension of sodium hydride in mineral oil and the mixture heated under reflux for 23 hours. The hot solution is filtered, concentrated in vacuo where upon the residue is crystallized. The mineral oil is removed by decantation and the solid washed with hexanes and dried in vacuo to give 45.5 g product which, by nmr analysis, is approximately 90% the desired isomer II and 10% the undesired isomer IIa.
A pure sample of isomer II can be obtained by recrystallizing the crude product from hexane- methylene chloride mp 107-1150C.
The cyclisation can be achieved by either the basic reagent sodium and potassium hydroxide, or the acidic reagent p-toluenesulfonic acid in a toluene solvent. It should be understood that a mixture of products corresponding to Structures II and IIa above is obtained and in general these are -not purified but used directly for the preparation of the derived nicotinic acid esters.
Employing the appropriate pyrrolopyridine carboxamide, the following imidazopyrrolopyridines are prepared.
A mixture containing 52 g of 3-[(1-Carbamoyl-1,2-dimethylpropyl)picolinate, 1.77 ml 1,5-diazabicyclo-[5.4.0]-undec-5-ene(DBU) in 400 ml xylene is heated under reflux under a Dean-Stark water separator for 2 hours. The mixture is concentrated in vacuo and the residue is chromatographed on 400 g basic alumina. The mixture of desired products is eluted with methylene chloride and used without further purification.
To 20 ml dry methanol in which 10 mg sodium hydride had reacted is added 2.0 g of a mixture of the imidazopyrrolopyridines. After stirring for 16 hours, 0.03 g glacial acetic acid is added (to neutralize the base), the solution concentrated in vacuo and the residue chromatographed on silica gel in ether. The faster moving material, the desired ester, is obtained in several fractions, combined, concentrated and crystallized from acetonitrile to give the imidazolinyl nicotinate, mp 121-123.5°C. An analytically pure sample crystallized from methylene chloride hexane exhibits a melting point of from 121-122°C.
This method involves the formation of the tricyclic compounds of Example 3 and 4, without isolation, directly forming the nicotinic acid esters:
A mixture of 25 g amide and 1 ml 1,5-diaza-- bicyclo-[5.4.0]undec-5-ene(DBU) in 500 ml xylene is heated under reflux for 1 hour under a Dean-Stark water separator. The mixture is cooled somewhat, the water separator removed, 100 ml anhydrous methanol added and the mixture heated under reflux for 1 hour. The solvents are then removed in vacuo and the product isolated by chromatography as described in Example 5 above to give 13.65 g product mp 120-122°C identical to that described in Example 5 above.
A mixture of 13.65 g of the nicotinate and 9.69 g phosphorus pentachloride in 110 ml dry toluene is heated with stirring to 80°C. After 1 1/2 hours, the thick mixture is cooled, filtered and the solid washed with ether and dried. This is the hydrochloride salt of the desired product.
This salt is dissolved in 60 ml water; ' neutralized with sodium bicarbonate, the resulting precipitate removed by filtration, washed with water and air-dried to give the product identical to that prepared by the procedure of Example 5.
A mixture of 5.0 g nicotinate and 7.1 g phosphorus pentachloride in 40 ml phosphorus oxychloride is stirred at room temperature overnight. The phosphorous oxychloride is removed in vacuo, the residue suspended in 40 ml toluene and again concentrated. This is repeated. Water (40 ml) is added to the residue and the mixture heated to reflux and held there for 1 hour. After cooling, the mixture is extracted with methylene chloride, the extract dried and concentrated to give 1.05 g of the desired product. The pH of the aqueous phase from the methylene chloride extraction is adjusted to 5-6 with sodium bicarbonate solution and the mixture extracted again with methylene chloride. The dried extract was concentrated and the residue crystallized to give a further 2.65 g of the desired product identical to that described in Example 5.
The following nicotinic acid esters are prepared by one or more of the methods described above:
To a stirred suspension of 3.0 g of the ester of Example 5 in 40 ml ether is added enough methylene chloride to obtain a solution. Dry hydrogen chloride is then passed into the solution for about 20 minutes. After 1 hour the mixture is filtered to remove the product which is washed with ether and dried to give 1.90 g of analytically pure hydrochloride salt and melting point equal to 195-196°C.
To 22.63 g ester of Example 5 in 100 ml water is added a solution containing 3.29 g sodium hydroxide in 25 ml water and the mixture heated under reflux with stirring for 1.5 hours. After standing at room temperature overnight, 6.8 ml concentrated hydrochloric acid is added causing a heavy precipitate to form. This is removed by filtration, washed with 20 ml water, followed by 30 ml ether and dried to give 19.27 g acid, , mp 168-170°C. This material is dissolved in 350 ml methylene chloride, filtered (to remove a small amount of the isomeric 2-acid) and concentrated to give 17.91 g of pure acid, mp 170-172°C. The analytically pure sample is prepared by recrystallization of the material from acetone-hexane, mp 170-172.5°C.
To 1.0 g of the benzyl ester in 20 ml ethanol is added 50 mg 5% palladium on carbon catalyst and the mixture shaken in an atmosphere of hydrogen until one equivalent of hydrogen has been absorbed. The catalyst is removed by filtration, the solvent removed in vacuo and the residue crystallized from acetone-hexane to give the acid as described in Example 9.
The following acids are made by the above methods:,
To 0.98 g of the acid of Example 9 partially dissolved in 10 ml water is added, with stirring 0.18 g calcium carbonate. After 10 minutes, the solution is filtered, the filtrate concentrated and the residue treated with ether to give a crystalline product which is dried at 40°C and 25 mm pressure to give 0.88 g of the calcium salt mp 265°C.
The sodium, diisopropylammonium, and triethylammonium salts are prepared in a similar manner.
The following salts can be prepared by the above procedure using the appropriate acid and the oxide, carbonate, bicarbonate or hydroxide of the selected metal, alkali metal, alkaline earth metal, ammonia or aliphatic amine.
To a stirred mixture containing 25.5 g acid chloride [(Helv. Chem. Acta, 34, 488 (1951)] and 29.7 ml triethylamine in 200 ml methylene chloride is added, under nitrogen, dropwise a solution containing 13.93 g amino amide as disclosed in (U.S. 4,017,510) at such a rate that the temperature of the reaction mixture remains below 30°C. After 1 hour, the mixture is filtered, the solid washed with methylene chloride and dried to give 19.8 g product, mp 176-177°C (decomp). A sample recrystallized from nitromethane had mp 196-196.5°C (decomp) and analytically pure.
To a stirred suspension of 18.4 g of the anhydride in 760 ml of dry acetone is added, under nitrogen, 16.2 g of (+) -methylvaline. After stirring at room temperature for 48 hours, the mixture is filtered and the filtrate concentrated to give the crude intermediate. This material is dissolved in 500 ml acetic anhydride, a catalytic quantity of sodium acetate added and the mixture stirred at room temperature for 5 hours. After heating under reflux for 1.5 hours, the mixture is concentrated. The residue is dissolved in ethyl acetate and washed with water. The dried extract is concentrated to give a dark syrup. A sample is dissolved in ethyl acetate, treated with charcoal, filtered and concentrated. The residue is crystallized from methylene chloride to give the product, mp 122 - 125°C
By essentially the same procedure and using the appropriate starting quinolinic anhydride and amino acid the following amides are prepared
By essentially the same procedure and using the appropriate acid, the following amides are prepared.
Using essentially the same procedures described above the following picolinic acids are prepared.
The residue is recrystallized from hexane to give the pure product mp 60-62°C.
- Using essentially the same procedure, the following imidazolinones are prepared.
Using essentially the same procedure as described above but substituting the appropriate imidazolinone for 2-(5-butyl-2-pyridyl)-5-isopropyl-5-methyl-2-imidazolinone, and using dimethylformamide and methyl iodide as well as carbon dioxide as electrophiles, the following imidazolinones are prepared:
A solution of the acid in ether is treated with excess diazomethane. After a few minutes excess diazomethane is removed by warming. The solvent is removed and the residue crystallized from ether hexane to give the desired methyl ester mp 128-13°C.
Using essentially the same conditions as those described above, the following methyl esters are prepared starting with the appropriate acid.
The cyclization of the amide is accomplished by heating 7.83 g of amide in 150 ml of toluene and 0.45 ml of 1,8-diazabicyclo[5,4,0]undec-7-ene under a Dean-Stark water Separator for 2 hours as described in Example 4. The separator is removed, 4 ml of morpholine is added and heating continued for 3 hours. The mixture is concentrated and the residue chromatographed on silica gel in ethyl acetate. The product is eluted first and this material is recrystallized from ether-hexane to give pure amide mp 143-145.5°C.
By substituting the appropriate amine for morpholine, the following amides are prepared.
A solution containing 10.0 g of ester in , 50 ml of tetrahydrofuran is added to 100 ml of liquid ammonia in a glass bomb. The bomb is sealed and the contents heated at 1000C for 16 hours. After cooling, the ammonia is evaporated and the residue concentrated. This residue is combined with material from similar reactions using 5 g and 7 g of the ester. These are crystallized from ethyl acetate to give 5 g of product. The filtrate is concentrated after treatment with charcoal to give a further 15.7 g of product.
Two recrystallizations of a sample from ethyl acetate gives the pure nicotinamide as a white crystalline solid mp 178-182°C.
To 75 ml of ice-cold tetrahydrofuran under nitrogen is added with stirring 12 ml of titanium tetrachloride in 20 ml of carbon tetrachloride at such a rate that the temperature does not exceed 50C. This is followed by the addition of 5.2 g of the amide in 75 ml of tetrahydrofuran again maintaining a temperature of <5°C. Finally, 17 ml of triethylamine in 5 ml of tetrahydrofuran is added to the mixture under the same conditions. After 1.5 hours at 50C, the mixture is stirred overnight at room temperature. Water (100 ml) is cautiously added at 0°C, the upper organic phase is separated and the aqueous phase extracted with methylene chloride (4x100 ml). The combined extracts are washed with brine, dried and concentrated. The solid residue is recrystallized from hexane-methylene-chloride to give the nicotinonitrile as a tan solid, mp 144-1480C. The analytically pure compound has mp 148-150°C.
To a stirred slurry of 23 g lithium aluminum hydride in 250 ml tetrahydrofuran under nitrogen at -70oC is added dropwise 46.8 g of the ester in 350 ml tetrahydrofuran. The mixture is warmed to room temperature, 73 ml of a saturated ammonium chloride solution added cautiously with vigorous stirring, the mixture filtered and the solid washed with tetrahydrofuran. The filtrate is concentrated to leave a gum. This is chromato- 'graphed on silica gel and the product eluted by ethyl acetate, mp 101-104°C.
To a stirred solution containing 2.03 g alcohol in 3.5 ml dimethylformamide under nitrogen is added 0.68 g imidazole followed by 3.1 g t-butyldimethylsilyl chloride. The mixture is kept at 35°C for 10 hours and room temperature for 10 hours. Saturated sodium sulfate is added and the aqueous mixture extracted with ether. The extract is washed with brine, dried and evaporated. The pure product is isolated as a gum by chromatography of the crude product on silica gel and elution with methylene chloride followed by ether.
Using essentially the same conditions as those described above, the following N-substituted imidazolinones are prepared by reacting the appropriate imidazolinyl nicotinate with the appropriate acyl anhydride, acyl halide, sulfonyl halide, alkyl halide or sulfate either alone or in a solvent such as pyridine or toluene.
To a solution of 30 g of the N-acetyl compound in 200 ml methanol is added approximately 0.5 g sodium methoxide. After stirring for two hours, the product is removed by filtration and air dried, mp 197-201°C. The analytically pure sample which had been recrystallized from acetone-hexane has mp 200-201°C.
A solution containing 22.0 g N-oxide in 135 ml phosphorus oxychloride is heated under reflux for four hours. After standing at room temperature overnight, excess phosphorus oxychloride is removed in vacuo and the residue treated with xylene and again concentrated. The residue is dissolved in methylene chloride and treated with water, the pH adjusted to 5 with sodium carbonate and ether added to make the organic layer the upper layer. The layers are separated and the aqueous phase reextracted twice with ether. The combined organic extract$ are washed with brine, dried and concentrated. The residue is chromatographed on 250 g of silica gel in a mixture of ether and hexane to give 10.6 g of the desired product. This is recrystallized from ether-hexane to give 8.95 g of the 6-chloro derivative, mp 104-106°C. The analytically pure sample melted at 102.5-104.5°C.
Following the above procedure but substituting the 5-bromo ester for the 6-chloro ester yields 5-bromo-2-(5-isopropyl-5-methyl-4-oxo-2-imidazolin-2-yl)nicotinic acid, mp 211-213°C.
After cooling, the mixture is diluted with water, the pH thereof adjusted to 1 with 1N hydrochloric acid and then back to pH 8 with saturated sodium bicarbonate. The mixture is extracted twice with ether and the ether discarded. The pH of the aqueous phase is adjusted to 5 and extracted several times with methylene chloride. The extracts are combined, dried and concentrated. Crystallization from ether-hexane gives the 6-benzyloxy derivative mp 205 - 207°C.
Using essentially the same conditions as described above, and using the appropriate 4- or 6-chloro-2-(5-isopropyl-5-methyl-4-oxo-2-imidazolin-2-yl)nicotinic acid and appropriate sodium alkoxide, phenoxide or thioalkoxide, the following imidazolinyl nicotinic acids are prepared.
To a solution containing 50.9 g of dicyclohexylcarbodiimide in 600 ml of dry methylene chloride is added, while stirring, 60 g of the acid at such a rate that the temperature does not exceed 320C. After stirring at room temperature for 2.5 hours, the mixture is filtered and the filtrate concentrated to give a white solid. This solid is recrystallized from methylene chloride to give 57.4 g of the dione, mp 125-128.5°C. The analytically pure dione melts at 132-134°C.
Using essentially the same procedure as described above but substituting phenyl lithium or - sodium trimethyl phosphonoacetate for methyl magnesium bromide, the following imidazolinones are prepared.
To a stirred solution of 0.32 g sodium borohydride in 25 ml absolute ethanol at 0°C is added during 10 minutes with stirring a solution containing 2.0 g dione in 25 ml dry tetrahydrofuran. The mixture is then stirred a further three hours at room temperature. The mixture is poured into 200 ml ice water, extracted with methylene chloride, the extract dried and concentrated. The residue is crystallized from methylene-chloride-hexane to give the desired product. The analytically pure sample has mp 145-149°C.
Employing similar conditions the compounds of Table I are prepared.
Other compounds prepared by this procedure are listed in Table I.
The following compounds are prepared in the same manner as described above.
This example is used to prepare the following examples. However to avoid ester formation, rather than filter off the insolubles and strip down the xylene one may simply add methanol followed by water (caution hydrogen may be evolved) to the xylene layer.
To 2-isopropyl-2-methyl-5-H-imidazo[1',2':1,2] pyrazolo [3,4-b]quinoline-3H(2H),5-dione (2 g, 0.0068 mol) in absolute ethanol (40 ml) under nitrogen is added, 50% sodium hydride (0.34 g, 0.00716 mol) with icecooling. Gas evolution is observed. After 10 minutes the reaction is neutralized with aqueous ammonium chloride, stripped and partitioned between water and ethyl acetate. The organic layer is separated and dried over anhydrous magnesium sulfate, filtered, stripped and the residue crystallized from ethyl acetate-hexane to give 1.38 g (60%) of a white solid, mp 146-147.5°C.
In a similar manner, the following esters in Table IV may be prepared by Procedure A.
A 50% sodium hydride oil dispersion (1.4 g, 0.0292 mol) is added to azeotropically dried 1,3-dihydro- a-isopropyl-a-methyl-l,3-dioxo-2-H-pyrrolo [3,4-b ] quinoline-2-acetamide (6 g, 0.0193 mol) under nitrogen. The mixture is heated and stirred under reflux for 6 hours, cooled and slowly quenched with a solution of sodium methoxide (0.1 g) in methanol (20 ml). After heating at 60°C for 3 hours the mixture is filtered and the filtrate stripped to give a white solid, which is dissolved in a methylene chloride-water mixture. Separation of the organic layer and stripping afforded a solid of 0.48 g, which is purified by passing through a silica gel pad with ethyl acetate as solvent. After removal of the solvent, the solid residue is crystallized from ethyl acetate-hexane to give white needles of the required ester, 0.4 g, mp 145-154°C. Anal. calcd. for C18H19N3O3: C, 66.44; H, 5.89; N, 12.92. Found: C, 66.35; H, 5.93; N, 12.83.
A solution of the ester is dissolved in an ether-methylene chloride solution and dry hydrogen chloride is passed through the solution until all of the solid hydrochloride salt forms and is filtered off, ether washed and vacuum dried, with mp 226-270°C. The following salts may be prepared in a similar manner, substituting the appropriate acid HX, although some are hygroscopic oils, and employing ethyl acetate as a preferred solvent for acid salts.
Substituting for sodium hydroxide the following salts are prepared in a similar manner. Compounds prepared in this manner are described in Table VI.
Other tricycles are obtained by procedures similar to Procedures A and B above.
Examples of Tricycles:
Pyridinium chlorochromate (4.4 g, 0.0204 mol) in methylene dichloride (20 ml) is added rapidly to a methylene chloride (20 ml) solution of N-(l-cyano-1,2-dimethylpropyl)-2-(5-chloro-2-hydroxymethylanilino)maleimide (4.75 g, 0.0136 mol). After 2 hours the dark reaction mixture is diluted with ether (20 ml) and a yellow precipitate is formed and is filtered off. This solid is redissolved in ethyl acetate:methylene chloride (1:1) and is passed through a-silica gel column to give a yellow solid 4.31 g, (92%) with mp 80°C (dec.).
The following aldehydes are prepared according to Procedures A or B as shown in Table VII.
To o-aminobenzyl alcohol, (2 g, 0.0125 mol) and 3-bromo- -isopropyl- -methyl-2,5-dioxo-3-pyrroline-1-acetonitrile (2.7 g, 0.01 mol) is added absolute ethanoL (100 ml) containing 3 g of 5 A pulverized sieves. The mixture is stirred for 20 hours at room temperature. The solvent is removed and the residue is purified through a silica gel dry column, eluant ether-hexane (2:1). Starting bromomaleimide is first recovered, followed by a bright yellow solid 1.89 g (60%), mp 39-45°C. Anal. calcd. for C17H19N303: C, 65.16; H, 6.11; N, 13.41. Found: C, 65.94; H, 6.21; N, 12.87.
Other compounds are prepared by the above procedure with variously substituted o-aminobenzyl-alcohols. Employing i-propanol or t-butanol for ethanol generally improves the product yield and bases as acid acceptors may also be employed.
Other bromomaleimides are prepared in a similar manner.
In a similar manner the following compounds are prepared.
In a similar manner other maleimides may be prepared.
In a similar manner other quinolinecarboxamides may be prepared.
In a similar manner other imideamides are prepared.
Other compounds prepared in a similar manner are shown in Table IX..
*Dilution of the 95% ethanol with 5-10 ml of water gives a new solid which is filtered off. Washing the solid with 95% ethanol removes the yellow color and gives a material mp 168-169°C. An m+1 confirms either tricyclic structure A or B. e
In a similar manner other A groups are prepared.
Powdered sodium borohydride (0.5 g, 0.013 mol) is added to 2-(5-isopropyl-5-methyl-4-oxo-2-imidazolin-2-yl)-3-quinolinecarboxaldehyde (0.78 g, 0.00264 mol) which is suspended in ethanol (150 ml) under nitrogen. A clear yellow solution results. After 20 minutes the reaction is concentrated to 40 ml, and is diluted with water (75 ml). Methylene chloride extraction and evaporation gives a solid which is crystallized from hexane-ethyl acetate to give pale yellow crystals, mp 138-149°C, M/e 298.
Other compounds may be prepared by the above procedure using the appropriately substituted quinoline- carboxaldehyde. Such compounds are shown in Table X.
In the following tests, the appropriate compounds are dissolved or dispersed in acetone-water (1:1) mixtures at the final concentration corresponding to the kg/ha rates indicated in the tables below. The solutions also contain 0.1% to 0.25% v/v colloidal BIOFILM® product of Colloidal Products Corp.) which is a mixture of alkyl aryl polyethoxyethanol, free and combined fatty acids, glycol ethers, dialkylbenzene carboxylate and 2-propanol.
. The plant species used in these tests is , barley (Hordeum vulgare, var. Mexico).
The solution or dispersion of the compound under test is sprayed at a rate of 40 ml per pot applied to the foliage.
In the'postemergence tests, the plants are seedlings at the two leaf stage (19 plants per pot; pot size; 8.9 cm x 6.3 cm x 6.3 cm).
The pots were watered immediately before treatment and placed on benches in a random arrangement in the greenhouse. Normal watering and fertilizing practices are followed (pesticides are applied to the plants as needed).- Minimum day and night temperatures of 18. 3° C are maintained during cooler periods of the year. Normal daily fluctuations occur during the summer season.
Plants are sprayed to provide the kg/ha rates indicated in the tables below. Each treatment is replicated 6 times.
Periodic observations are made after treatment and morphological changes are noted. From these observations as compared to the untreated controls, the maturation effect of the instant compounds on barley can be determined.
The data thus obtained are averaged and summarized in Tables Ia to Ic inclusive.
Evaluation of the increased branching and flowering effect of the compounds or in the invention. ,
By the method of Example 60, the effect of the compounds of the invention is evaluated on soybeans (Glycine max, var. ADELPHI). Each test is replicated six times. There is one plant per pot and the plants are treated at the 2nd to 3rd trifoliate stage.
The data obtained are averaged and summarized in Tables IIa to IIc inclusive.
By the method of Example 60, the effect of the compounds of the invention is evaluated on soybeans (Glycine max. var. ADELPHI). Each test is replicated 6 times. There is one plant per pot and the plants are treated at the 6th trifoliate stage. The data obtained are averaged and summarized in Table XI below.
By the method of Example 60, wheat (Triticum aestivum, var. Era) is treated at the early tillering stage at the kg/ha rates indicated in Table XII below. Each treatment is replicated 6 times.
The plants are harvested five weeks post treatment, the number of heads, fresh and dry head weight and straw weight are determined and the % changes (+) indicated. The data obtained are averaged and summarized in Table XII below.
The seeds of wheat (Triticum aestivum, var. Era) are mixed with potting soil and planted on top of approximately 2.5 cm of soil in 12.5 cm diameter fiber pots. After planting, the pots are sprayed with the aqueous acetone solution containing the test compound at the kg/ha rates given in Table V below. The treated cups are then placed on greenhouse benches, watered and cared for in accordance with conventional greenhouse procedures. Each treatment is replicated six times.
The plants are harvested 11 weeks post treatment, the number of heads, fresh and dry head weight and straw weight are determined and the % changes (+) indicated. The data obtained are averaged and summarized in Table XII below.
In the following tests, seeds of either Era variety wheat or Adelphi variety soybeans, are planted in 13 cm fiber pots. The test compounds are then dispersed in acetone-water (1:1) mixtures containing 0.1% to 0.25% v/v colloidal BIOFILM which is a mixture of alkyl aryl polyethoxyethanol, free and combined fatty acids, glycol ethers, dialkylbenzene carboxylate and 2-propanol. The planted seeds are sprayed with a sufficient amount of the mixture to provide each pot with 0.1, 0.01, or 0.001 kg/ha of test compound. The treated cups are then placed on greenhouse benches and cared for in accordance with greenhouse procedures. Three months after treatment the soybeans are harvested, dried and weighed. Six replicates per treatment are used and averaged. Data obtained are reported below.
The following tests were conducted using the procedure of Example 60, excepting that the plant species used is Era wheat and the crop is harvested nine weeks after treatment, the following results are obtained.
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